Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4599 and Pf6N2E2_4600 are separated by 10 nucleotides Pf6N2E2_4600 and Pf6N2E2_4601 are separated by 35 nucleotides Pf6N2E2_4601 and Pf6N2E2_4602 are separated by 53 nucleotides
Pf6N2E2_4599 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 5,325,694 to 5,326,284
_4599
Pf6N2E2_4600 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 5,326,295 to 5,327,113
_4600
Pf6N2E2_4601 - Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC, at 5,327,149 to 5,327,835
_4601
Pf6N2E2_4602 - Twitching motility protein PilT, at 5,327,889 to 5,328,923
_4602
Position (kb) 5327
5328 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.175 kb on + strand, within Pf6N2E2_4599 at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.288 kb on + strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.289 kb on - strand at 5326.296 kb on + strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.125 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.127 kb on - strand at 5327.663 kb on + strand, within Pf6N2E2_4601 at 5327.664 kb on - strand, within Pf6N2E2_4601 at 5327.664 kb on - strand, within Pf6N2E2_4601 at 5327.803 kb on - strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.835 kb on + strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.836 kb on - strand at 5327.869 kb on + strand at 5327.905 kb on + strand at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.996 kb on + strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5327.997 kb on - strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.042 kb on + strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.043 kb on - strand, within Pf6N2E2_4602 at 5328.070 kb on + strand, within Pf6N2E2_4602 at 5328.071 kb on - strand, within Pf6N2E2_4602 at 5328.075 kb on + strand, within Pf6N2E2_4602 at 5328.229 kb on + strand, within Pf6N2E2_4602 at 5328.229 kb on + strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.230 kb on - strand, within Pf6N2E2_4602 at 5328.325 kb on - strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.792 kb on + strand, within Pf6N2E2_4602 at 5328.793 kb on - strand, within Pf6N2E2_4602 at 5328.793 kb on - strand, within Pf6N2E2_4602
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 5,326,175 + Pf6N2E2_4599 0.81 -1.1 5,326,175 + Pf6N2E2_4599 0.81 -0.8 5,326,175 + Pf6N2E2_4599 0.81 +0.5 5,326,175 + Pf6N2E2_4599 0.81 -0.0 5,326,288 + +0.5 5,326,288 + -1.3 5,326,288 + +0.5 5,326,288 + -1.6 5,326,289 - -1.9 5,326,289 - -1.3 5,326,289 - -1.5 5,326,289 - -2.0 5,326,296 + -2.9 5,327,125 - -0.7 5,327,125 - +0.5 5,327,125 - -0.4 5,327,125 - -0.7 5,327,125 - -2.0 5,327,127 - +0.4 5,327,127 - +0.3 5,327,127 - -0.3 5,327,127 - -0.9 5,327,663 + Pf6N2E2_4601 0.75 -1.8 5,327,664 - Pf6N2E2_4601 0.75 -2.0 5,327,664 - Pf6N2E2_4601 0.75 +0.5 5,327,803 - +0.5 5,327,835 + -2.2 5,327,835 + -0.8 5,327,835 + -0.0 5,327,835 + -2.3 5,327,835 + -1.3 5,327,836 - +0.1 5,327,836 - +0.2 5,327,836 - -1.5 5,327,836 - +0.5 5,327,836 - +0.3 5,327,836 - +0.8 5,327,836 - +1.1 5,327,869 + +0.8 5,327,905 + -1.6 5,327,996 + Pf6N2E2_4602 0.10 -1.8 5,327,996 + Pf6N2E2_4602 0.10 +0.3 5,327,996 + Pf6N2E2_4602 0.10 +0.2 5,327,997 - Pf6N2E2_4602 0.10 -0.5 5,327,997 - Pf6N2E2_4602 0.10 -0.0 5,327,997 - Pf6N2E2_4602 0.10 -0.2 5,327,997 - Pf6N2E2_4602 0.10 -1.0 5,328,042 + Pf6N2E2_4602 0.15 -1.5 5,328,042 + Pf6N2E2_4602 0.15 +1.1 5,328,042 + Pf6N2E2_4602 0.15 +0.6 5,328,042 + Pf6N2E2_4602 0.15 -0.0 5,328,043 - Pf6N2E2_4602 0.15 +1.5 5,328,043 - Pf6N2E2_4602 0.15 +0.5 5,328,043 - Pf6N2E2_4602 0.15 -0.1 5,328,043 - Pf6N2E2_4602 0.15 +0.4 5,328,070 + Pf6N2E2_4602 0.17 +0.5 5,328,071 - Pf6N2E2_4602 0.18 -0.3 5,328,075 + Pf6N2E2_4602 0.18 +1.0 5,328,229 + Pf6N2E2_4602 0.33 +0.4 5,328,229 + Pf6N2E2_4602 0.33 +1.5 5,328,230 - Pf6N2E2_4602 0.33 +0.8 5,328,230 - Pf6N2E2_4602 0.33 -1.9 5,328,230 - Pf6N2E2_4602 0.33 +0.2 5,328,325 - Pf6N2E2_4602 0.42 -0.5 5,328,792 + Pf6N2E2_4602 0.87 +0.3 5,328,792 + Pf6N2E2_4602 0.87 +0.1 5,328,792 + Pf6N2E2_4602 0.87 -0.3 5,328,792 + Pf6N2E2_4602 0.87 -0.4 5,328,792 + Pf6N2E2_4602 0.87 +1.0 5,328,793 - Pf6N2E2_4602 0.87 -0.2 5,328,793 - Pf6N2E2_4602 0.87 +0.5
Or see this region's nucleotide sequence