Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4012 and Pf6N2E2_4013 are separated by 138 nucleotides Pf6N2E2_4013 and Pf6N2E2_4014 are separated by 173 nucleotides
Pf6N2E2_4012 - response regulator, at 4,683,332 to 4,684,549
_4012
Pf6N2E2_4013 - 5-aminovalerate transaminase (EC 2.6.1.48) (from data) , at 4,684,688 to 4,685,965
_4013
Pf6N2E2_4014 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 4,686,139 to 4,687,581
_4014
Position (kb)
4684
4685
4686 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4683.696 kb on + strand, within Pf6N2E2_4012 at 4683.697 kb on - strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.383 kb on + strand, within Pf6N2E2_4012 at 4684.384 kb on - strand, within Pf6N2E2_4012 at 4684.384 kb on - strand, within Pf6N2E2_4012 at 4684.524 kb on - strand at 4684.569 kb on + strand at 4684.569 kb on + strand at 4684.569 kb on + strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.570 kb on - strand at 4684.580 kb on + strand at 4684.581 kb on - strand at 4684.634 kb on + strand at 4684.635 kb on - strand at 4684.785 kb on + strand at 4684.785 kb on + strand at 4684.786 kb on - strand at 4684.786 kb on - strand at 4684.786 kb on - strand at 4684.893 kb on - strand, within Pf6N2E2_4013 at 4684.938 kb on + strand, within Pf6N2E2_4013 at 4684.939 kb on - strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.070 kb on + strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.071 kb on - strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.109 kb on + strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.110 kb on - strand, within Pf6N2E2_4013 at 4685.315 kb on + strand, within Pf6N2E2_4013 at 4685.315 kb on + strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.316 kb on - strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.319 kb on + strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.320 kb on - strand, within Pf6N2E2_4013 at 4685.339 kb on + strand, within Pf6N2E2_4013 at 4685.451 kb on + strand, within Pf6N2E2_4013 at 4685.488 kb on + strand, within Pf6N2E2_4013 at 4685.500 kb on - strand, within Pf6N2E2_4013 at 4685.500 kb on - strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.706 kb on + strand, within Pf6N2E2_4013 at 4685.715 kb on + strand, within Pf6N2E2_4013 at 4685.929 kb on + strand at 4685.930 kb on - strand at 4685.966 kb on + strand at 4685.967 kb on - strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.012 kb on + strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.013 kb on - strand at 4686.158 kb on + strand at 4686.158 kb on + strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.159 kb on - strand at 4686.174 kb on - strand at 4686.188 kb on + strand at 4686.188 kb on + strand at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.332 kb on + strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.333 kb on - strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.338 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.562 kb on + strand, within Pf6N2E2_4014 at 4686.563 kb on - strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.677 kb on + strand, within Pf6N2E2_4014 at 4686.695 kb on + strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.696 kb on - strand, within Pf6N2E2_4014 at 4686.768 kb on + strand, within Pf6N2E2_4014 at 4686.796 kb on - strand, within Pf6N2E2_4014
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 4,683,696 + Pf6N2E2_4012 0.30 +0.9 4,683,697 - Pf6N2E2_4012 0.30 -0.2 4,684,383 + Pf6N2E2_4012 0.86 +0.1 4,684,383 + Pf6N2E2_4012 0.86 +0.4 4,684,383 + Pf6N2E2_4012 0.86 -0.6 4,684,383 + Pf6N2E2_4012 0.86 +1.2 4,684,384 - Pf6N2E2_4012 0.86 -1.5 4,684,384 - Pf6N2E2_4012 0.86 +2.3 4,684,524 - -1.0 4,684,569 + +0.4 4,684,569 + +0.8 4,684,569 + +1.0 4,684,570 - -1.6 4,684,570 - +0.6 4,684,570 - -0.8 4,684,570 - -0.2 4,684,580 + -0.4 4,684,581 - +0.2 4,684,634 + +0.3 4,684,635 - -2.6 4,684,785 + -0.0 4,684,785 + -1.2 4,684,786 - -0.0 4,684,786 - +0.3 4,684,786 - +0.7 4,684,893 - Pf6N2E2_4013 0.16 -2.1 4,684,938 + Pf6N2E2_4013 0.20 +0.2 4,684,939 - Pf6N2E2_4013 0.20 -2.0 4,685,070 + Pf6N2E2_4013 0.30 +0.6 4,685,070 + Pf6N2E2_4013 0.30 -1.0 4,685,070 + Pf6N2E2_4013 0.30 -1.2 4,685,071 - Pf6N2E2_4013 0.30 +0.6 4,685,071 - Pf6N2E2_4013 0.30 +1.6 4,685,071 - Pf6N2E2_4013 0.30 -2.5 4,685,071 - Pf6N2E2_4013 0.30 -0.4 4,685,071 - Pf6N2E2_4013 0.30 -1.0 4,685,109 + Pf6N2E2_4013 0.33 -1.2 4,685,109 + Pf6N2E2_4013 0.33 -1.6 4,685,109 + Pf6N2E2_4013 0.33 +0.6 4,685,110 - Pf6N2E2_4013 0.33 -1.6 4,685,110 - Pf6N2E2_4013 0.33 -2.2 4,685,110 - Pf6N2E2_4013 0.33 -2.1 4,685,110 - Pf6N2E2_4013 0.33 -0.0 4,685,315 + Pf6N2E2_4013 0.49 +1.6 4,685,315 + Pf6N2E2_4013 0.49 -0.0 4,685,316 - Pf6N2E2_4013 0.49 +1.0 4,685,316 - Pf6N2E2_4013 0.49 -0.8 4,685,316 - Pf6N2E2_4013 0.49 -2.1 4,685,316 - Pf6N2E2_4013 0.49 -2.3 4,685,316 - Pf6N2E2_4013 0.49 +0.1 4,685,316 - Pf6N2E2_4013 0.49 -1.2 4,685,316 - Pf6N2E2_4013 0.49 +0.6 4,685,316 - Pf6N2E2_4013 0.49 -1.2 4,685,319 + Pf6N2E2_4013 0.49 -0.8 4,685,319 + Pf6N2E2_4013 0.49 +0.6 4,685,319 + Pf6N2E2_4013 0.49 +0.2 4,685,319 + Pf6N2E2_4013 0.49 -0.8 4,685,319 + Pf6N2E2_4013 0.49 -1.4 4,685,319 + Pf6N2E2_4013 0.49 -0.4 4,685,319 + Pf6N2E2_4013 0.49 -1.6 4,685,319 + Pf6N2E2_4013 0.49 -1.6 4,685,319 + Pf6N2E2_4013 0.49 -3.1 4,685,320 - Pf6N2E2_4013 0.49 +0.6 4,685,320 - Pf6N2E2_4013 0.49 -1.8 4,685,320 - Pf6N2E2_4013 0.49 -0.8 4,685,320 - Pf6N2E2_4013 0.49 -0.8 4,685,320 - Pf6N2E2_4013 0.49 -1.8 4,685,320 - Pf6N2E2_4013 0.49 -1.1 4,685,339 + Pf6N2E2_4013 0.51 -0.4 4,685,451 + Pf6N2E2_4013 0.60 -0.0 4,685,488 + Pf6N2E2_4013 0.63 +1.6 4,685,500 - Pf6N2E2_4013 0.64 -1.4 4,685,500 - Pf6N2E2_4013 0.64 -1.4 4,685,706 + Pf6N2E2_4013 0.80 -1.2 4,685,706 + Pf6N2E2_4013 0.80 -0.8 4,685,706 + Pf6N2E2_4013 0.80 -0.0 4,685,715 + Pf6N2E2_4013 0.80 -1.0 4,685,929 + +2.6 4,685,930 - +1.6 4,685,966 + -1.8 4,685,967 - +0.8 4,686,012 + -1.9 4,686,012 + -2.3 4,686,012 + -1.2 4,686,012 + -0.4 4,686,012 + +0.7 4,686,013 - -0.4 4,686,013 - +0.0 4,686,013 - +0.2 4,686,013 - -0.6 4,686,013 - -1.3 4,686,013 - +0.6 4,686,013 - -0.4 4,686,013 - +0.1 4,686,013 - -0.8 4,686,013 - +0.6 4,686,013 - -0.8 4,686,158 + +0.6 4,686,158 + +1.6 4,686,159 - -0.6 4,686,159 - -1.4 4,686,159 - -3.4 4,686,159 - -3.0 4,686,159 - +0.6 4,686,174 - -2.2 4,686,188 + -1.7 4,686,188 + +1.6 4,686,332 + Pf6N2E2_4014 0.13 +0.6 4,686,332 + Pf6N2E2_4014 0.13 -2.6 4,686,332 + Pf6N2E2_4014 0.13 -0.4 4,686,332 + Pf6N2E2_4014 0.13 -0.0 4,686,332 + Pf6N2E2_4014 0.13 -1.9 4,686,333 - Pf6N2E2_4014 0.13 -0.0 4,686,333 - Pf6N2E2_4014 0.13 -1.0 4,686,333 - Pf6N2E2_4014 0.13 -3.6 4,686,333 - Pf6N2E2_4014 0.13 -1.4 4,686,333 - Pf6N2E2_4014 0.13 -2.0 4,686,333 - Pf6N2E2_4014 0.13 -0.4 4,686,338 + Pf6N2E2_4014 0.14 -1.0 4,686,338 + Pf6N2E2_4014 0.14 -0.6 4,686,338 + Pf6N2E2_4014 0.14 -1.6 4,686,338 + Pf6N2E2_4014 0.14 -0.8 4,686,338 + Pf6N2E2_4014 0.14 -0.0 4,686,338 + Pf6N2E2_4014 0.14 -2.5 4,686,562 + Pf6N2E2_4014 0.29 -2.6 4,686,562 + Pf6N2E2_4014 0.29 -1.0 4,686,562 + Pf6N2E2_4014 0.29 -0.4 4,686,562 + Pf6N2E2_4014 0.29 -0.0 4,686,563 - Pf6N2E2_4014 0.29 -0.6 4,686,677 + Pf6N2E2_4014 0.37 -1.0 4,686,677 + Pf6N2E2_4014 0.37 -1.1 4,686,677 + Pf6N2E2_4014 0.37 -0.4 4,686,695 + Pf6N2E2_4014 0.39 -0.8 4,686,696 - Pf6N2E2_4014 0.39 -0.4 4,686,696 - Pf6N2E2_4014 0.39 -1.6 4,686,696 - Pf6N2E2_4014 0.39 -0.2 4,686,696 - Pf6N2E2_4014 0.39 -1.0 4,686,768 + Pf6N2E2_4014 0.44 -2.4 4,686,796 - Pf6N2E2_4014 0.46 -0.8
Or see this region's nucleotide sequence