Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1060 and Pf6N2E2_1061 overlap by 26 nucleotides Pf6N2E2_1061 and Pf6N2E2_1062 are separated by 130 nucleotides Pf6N2E2_1062 and Pf6N2E2_1063 are separated by 23 nucleotides Pf6N2E2_1063 and Pf6N2E2_1064 are separated by 159 nucleotides
Pf6N2E2_1060 - Pirin, at 1,237,290 to 1,237,985
_1060
Pf6N2E2_1061 - hypothetical protein, at 1,237,960 to 1,238,091
_1061
Pf6N2E2_1062 - UvrD/REP helicase family protein, at 1,238,222 to 1,240,708
_1062
Pf6N2E2_1063 - hypothetical protein, at 1,240,732 to 1,240,860
_1063
Pf6N2E2_1064 - hypothetical protein, at 1,241,020 to 1,242,369
_1064
Position (kb)
1238
1239
1240
1241 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1237.344 kb on + strand at 1237.456 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.638 kb on + strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.639 kb on - strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.675 kb on + strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.676 kb on - strand, within Pf6N2E2_1060 at 1237.716 kb on + strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.717 kb on - strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.786 kb on + strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.787 kb on - strand, within Pf6N2E2_1060 at 1237.900 kb on + strand, within Pf6N2E2_1060 at 1237.991 kb on + strand, within Pf6N2E2_1061 at 1238.031 kb on + strand, within Pf6N2E2_1061 at 1238.131 kb on + strand at 1238.186 kb on + strand at 1238.186 kb on + strand at 1238.187 kb on - strand at 1238.259 kb on + strand at 1238.260 kb on - strand at 1238.390 kb on - strand at 1238.390 kb on - strand at 1238.405 kb on + strand at 1238.405 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.411 kb on + strand at 1238.412 kb on - strand at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.824 kb on + strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062 at 1238.825 kb on - strand, within Pf6N2E2_1062 at 1239.029 kb on + strand, within Pf6N2E2_1062 at 1239.030 kb on - strand, within Pf6N2E2_1062 at 1239.030 kb on - strand, within Pf6N2E2_1062 at 1239.030 kb on - strand, within Pf6N2E2_1062 at 1239.032 kb on + strand, within Pf6N2E2_1062 at 1239.032 kb on + strand, within Pf6N2E2_1062 at 1239.032 kb on + strand, within Pf6N2E2_1062 at 1239.033 kb on - strand, within Pf6N2E2_1062 at 1239.132 kb on + strand, within Pf6N2E2_1062 at 1239.132 kb on + strand, within Pf6N2E2_1062 at 1239.133 kb on - strand, within Pf6N2E2_1062 at 1239.325 kb on - strand, within Pf6N2E2_1062 at 1239.325 kb on - strand, within Pf6N2E2_1062 at 1239.478 kb on - strand, within Pf6N2E2_1062 at 1239.524 kb on + strand, within Pf6N2E2_1062 at 1239.525 kb on - strand, within Pf6N2E2_1062 at 1239.525 kb on - strand, within Pf6N2E2_1062 at 1239.740 kb on + strand, within Pf6N2E2_1062 at 1239.741 kb on - strand, within Pf6N2E2_1062 at 1239.741 kb on - strand, within Pf6N2E2_1062 at 1239.741 kb on - strand, within Pf6N2E2_1062 at 1239.897 kb on + strand, within Pf6N2E2_1062 at 1239.897 kb on + strand, within Pf6N2E2_1062 at 1239.983 kb on + strand, within Pf6N2E2_1062 at 1239.984 kb on - strand, within Pf6N2E2_1062 at 1239.984 kb on - strand, within Pf6N2E2_1062 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.076 kb on + strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.077 kb on - strand, within Pf6N2E2_1062 at 1240.114 kb on + strand, within Pf6N2E2_1062 at 1240.115 kb on - strand at 1240.115 kb on - strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.376 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.451 kb on + strand, within Pf6N2E2_1062 at 1240.452 kb on - strand, within Pf6N2E2_1062 at 1240.483 kb on - strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.547 kb on + strand at 1240.580 kb on + strand at 1240.587 kb on - strand at 1240.587 kb on - strand at 1240.730 kb on - strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.738 kb on + strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.739 kb on - strand at 1240.744 kb on + strand at 1240.744 kb on + strand at 1240.745 kb on - strand, within Pf6N2E2_1063 at 1240.947 kb on + strand at 1241.051 kb on + strand at 1241.051 kb on + strand at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.263 kb on + strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.264 kb on - strand, within Pf6N2E2_1064 at 1241.277 kb on - strand, within Pf6N2E2_1064 at 1241.277 kb on - strand, within Pf6N2E2_1064 at 1241.284 kb on + strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.285 kb on - strand, within Pf6N2E2_1064 at 1241.348 kb on - strand, within Pf6N2E2_1064 at 1241.368 kb on + strand, within Pf6N2E2_1064 at 1241.391 kb on + strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.468 kb on - strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.482 kb on + strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.483 kb on - strand, within Pf6N2E2_1064 at 1241.484 kb on + strand, within Pf6N2E2_1064 at 1241.492 kb on + strand, within Pf6N2E2_1064 at 1241.500 kb on + strand, within Pf6N2E2_1064 at 1241.500 kb on + strand at 1241.501 kb on - strand, within Pf6N2E2_1064 at 1241.548 kb on + strand, within Pf6N2E2_1064 at 1241.548 kb on + strand, within Pf6N2E2_1064 at 1241.549 kb on - strand, within Pf6N2E2_1064 at 1241.549 kb on - strand, within Pf6N2E2_1064 at 1241.550 kb on + strand, within Pf6N2E2_1064 at 1241.551 kb on - strand, within Pf6N2E2_1064 at 1241.680 kb on + strand, within Pf6N2E2_1064
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 1,237,344 + +0.6 1,237,456 + Pf6N2E2_1060 0.24 -1.1 1,237,638 + Pf6N2E2_1060 0.50 -1.4 1,237,638 + Pf6N2E2_1060 0.50 -1.3 1,237,639 - Pf6N2E2_1060 0.50 +2.1 1,237,639 - Pf6N2E2_1060 0.50 +1.6 1,237,675 + Pf6N2E2_1060 0.55 -0.4 1,237,675 + Pf6N2E2_1060 0.55 -0.8 1,237,676 - Pf6N2E2_1060 0.55 -0.9 1,237,676 - Pf6N2E2_1060 0.55 -0.7 1,237,676 - Pf6N2E2_1060 0.55 -2.2 1,237,676 - Pf6N2E2_1060 0.55 -1.4 1,237,676 - Pf6N2E2_1060 0.55 -1.0 1,237,716 + Pf6N2E2_1060 0.61 -0.4 1,237,717 - Pf6N2E2_1060 0.61 +0.3 1,237,717 - Pf6N2E2_1060 0.61 -0.7 1,237,717 - Pf6N2E2_1060 0.61 +0.1 1,237,717 - Pf6N2E2_1060 0.61 -0.0 1,237,717 - Pf6N2E2_1060 0.61 +0.1 1,237,717 - Pf6N2E2_1060 0.61 +0.8 1,237,717 - Pf6N2E2_1060 0.61 -1.2 1,237,717 - Pf6N2E2_1060 0.61 -0.4 1,237,786 + Pf6N2E2_1060 0.71 +0.6 1,237,786 + Pf6N2E2_1060 0.71 -1.0 1,237,786 + Pf6N2E2_1060 0.71 -0.3 1,237,786 + Pf6N2E2_1060 0.71 -1.7 1,237,786 + Pf6N2E2_1060 0.71 +1.6 1,237,786 + Pf6N2E2_1060 0.71 +0.3 1,237,786 + Pf6N2E2_1060 0.71 -0.2 1,237,786 + Pf6N2E2_1060 0.71 -0.4 1,237,787 - Pf6N2E2_1060 0.71 -0.8 1,237,787 - Pf6N2E2_1060 0.71 -0.1 1,237,787 - Pf6N2E2_1060 0.71 -0.4 1,237,787 - Pf6N2E2_1060 0.71 -1.0 1,237,787 - Pf6N2E2_1060 0.71 +1.4 1,237,787 - Pf6N2E2_1060 0.71 +0.5 1,237,787 - Pf6N2E2_1060 0.71 +0.3 1,237,787 - Pf6N2E2_1060 0.71 -0.4 1,237,787 - Pf6N2E2_1060 0.71 -0.2 1,237,787 - Pf6N2E2_1060 0.71 -1.6 1,237,787 - Pf6N2E2_1060 0.71 -1.2 1,237,900 + Pf6N2E2_1060 0.88 -2.0 1,237,991 + Pf6N2E2_1061 0.23 +2.2 1,238,031 + Pf6N2E2_1061 0.54 -1.4 1,238,131 + -1.2 1,238,186 + -0.9 1,238,186 + -2.2 1,238,187 - +0.5 1,238,259 + +0.6 1,238,260 - +0.6 1,238,390 - -0.7 1,238,390 - +0.5 1,238,405 + -0.5 1,238,405 + -1.0 1,238,411 + +1.2 1,238,411 + -0.3 1,238,411 + +0.6 1,238,411 + -0.1 1,238,411 + +0.9 1,238,412 - +0.3 1,238,824 + Pf6N2E2_1062 0.24 -1.4 1,238,824 + Pf6N2E2_1062 0.24 +0.6 1,238,825 - Pf6N2E2_1062 0.24 -1.4 1,238,825 - Pf6N2E2_1062 0.24 -1.5 1,239,029 + Pf6N2E2_1062 0.32 +0.9 1,239,030 - Pf6N2E2_1062 0.32 +0.6 1,239,030 - Pf6N2E2_1062 0.32 +0.6 1,239,030 - Pf6N2E2_1062 0.32 -0.4 1,239,032 + Pf6N2E2_1062 0.33 +0.6 1,239,032 + Pf6N2E2_1062 0.33 -0.1 1,239,032 + Pf6N2E2_1062 0.33 +0.0 1,239,033 - Pf6N2E2_1062 0.33 -0.4 1,239,132 + Pf6N2E2_1062 0.37 -1.3 1,239,132 + Pf6N2E2_1062 0.37 +1.1 1,239,133 - Pf6N2E2_1062 0.37 -0.7 1,239,325 - Pf6N2E2_1062 0.44 +0.2 1,239,325 - Pf6N2E2_1062 0.44 +0.6 1,239,478 - Pf6N2E2_1062 0.51 +1.9 1,239,524 + Pf6N2E2_1062 0.52 -0.4 1,239,525 - Pf6N2E2_1062 0.52 +0.6 1,239,525 - Pf6N2E2_1062 0.52 -0.3 1,239,740 + Pf6N2E2_1062 0.61 +0.6 1,239,741 - Pf6N2E2_1062 0.61 -2.2 1,239,741 - Pf6N2E2_1062 0.61 -1.7 1,239,741 - Pf6N2E2_1062 0.61 -0.8 1,239,897 + Pf6N2E2_1062 0.67 +1.2 1,239,897 + Pf6N2E2_1062 0.67 -0.8 1,239,983 + Pf6N2E2_1062 0.71 +0.2 1,239,984 - Pf6N2E2_1062 0.71 +1.6 1,239,984 - Pf6N2E2_1062 0.71 -0.4 1,240,076 + Pf6N2E2_1062 0.75 +1.1 1,240,076 + Pf6N2E2_1062 0.75 -0.4 1,240,076 + Pf6N2E2_1062 0.75 +1.6 1,240,077 - Pf6N2E2_1062 0.75 -1.0 1,240,077 - Pf6N2E2_1062 0.75 +1.2 1,240,077 - Pf6N2E2_1062 0.75 -0.7 1,240,114 + Pf6N2E2_1062 0.76 +1.2 1,240,115 - +3.4 1,240,115 - Pf6N2E2_1062 0.76 -0.8 1,240,376 + Pf6N2E2_1062 0.87 -1.3 1,240,376 + Pf6N2E2_1062 0.87 +0.6 1,240,376 + Pf6N2E2_1062 0.87 -0.4 1,240,376 + Pf6N2E2_1062 0.87 +0.9 1,240,451 + Pf6N2E2_1062 0.90 -0.5 1,240,451 + Pf6N2E2_1062 0.90 -1.4 1,240,451 + Pf6N2E2_1062 0.90 +0.6 1,240,451 + Pf6N2E2_1062 0.90 -1.7 1,240,452 - Pf6N2E2_1062 0.90 -0.4 1,240,483 - -0.7 1,240,547 + -3.6 1,240,547 + +0.9 1,240,547 + +0.3 1,240,547 + -1.7 1,240,547 + -1.0 1,240,580 + +0.5 1,240,587 - +0.2 1,240,587 - +0.1 1,240,730 - +1.4 1,240,738 + +1.0 1,240,738 + -1.1 1,240,738 + +0.6 1,240,738 + +1.6 1,240,738 + +0.5 1,240,738 + -0.5 1,240,738 + -1.0 1,240,739 - +0.3 1,240,739 - +1.2 1,240,739 - +1.0 1,240,739 - +0.0 1,240,744 + -0.2 1,240,744 + +0.6 1,240,745 - Pf6N2E2_1063 0.10 +0.8 1,240,947 + +0.6 1,241,051 + +0.6 1,241,051 + -0.4 1,241,263 + Pf6N2E2_1064 0.18 +1.2 1,241,263 + Pf6N2E2_1064 0.18 +0.3 1,241,263 + Pf6N2E2_1064 0.18 +2.8 1,241,263 + Pf6N2E2_1064 0.18 +0.2 1,241,264 - Pf6N2E2_1064 0.18 +2.0 1,241,264 - Pf6N2E2_1064 0.18 -0.1 1,241,264 - Pf6N2E2_1064 0.18 -0.8 1,241,277 - Pf6N2E2_1064 0.19 +0.3 1,241,277 - Pf6N2E2_1064 0.19 +0.8 1,241,284 + Pf6N2E2_1064 0.20 +0.6 1,241,285 - Pf6N2E2_1064 0.20 +0.6 1,241,285 - Pf6N2E2_1064 0.20 -1.4 1,241,285 - Pf6N2E2_1064 0.20 +1.3 1,241,348 - Pf6N2E2_1064 0.24 +0.6 1,241,368 + Pf6N2E2_1064 0.26 +1.3 1,241,391 + Pf6N2E2_1064 0.27 +0.8 1,241,468 - Pf6N2E2_1064 0.33 +0.5 1,241,468 - Pf6N2E2_1064 0.33 -1.3 1,241,468 - Pf6N2E2_1064 0.33 -0.1 1,241,482 + Pf6N2E2_1064 0.34 +0.6 1,241,482 + Pf6N2E2_1064 0.34 +0.6 1,241,482 + Pf6N2E2_1064 0.34 -2.1 1,241,482 + Pf6N2E2_1064 0.34 -0.3 1,241,482 + Pf6N2E2_1064 0.34 +0.6 1,241,482 + Pf6N2E2_1064 0.34 +0.6 1,241,482 + Pf6N2E2_1064 0.34 +0.9 1,241,482 + Pf6N2E2_1064 0.34 +1.3 1,241,483 - Pf6N2E2_1064 0.34 -2.2 1,241,483 - Pf6N2E2_1064 0.34 +0.3 1,241,483 - Pf6N2E2_1064 0.34 +0.0 1,241,483 - Pf6N2E2_1064 0.34 +0.6 1,241,483 - Pf6N2E2_1064 0.34 +0.6 1,241,484 + Pf6N2E2_1064 0.34 -0.8 1,241,492 + Pf6N2E2_1064 0.35 -1.6 1,241,500 + Pf6N2E2_1064 0.36 -0.7 1,241,500 + +1.6 1,241,501 - Pf6N2E2_1064 0.36 +0.2 1,241,548 + Pf6N2E2_1064 0.39 +0.7 1,241,548 + Pf6N2E2_1064 0.39 +0.6 1,241,549 - Pf6N2E2_1064 0.39 -1.0 1,241,549 - Pf6N2E2_1064 0.39 -0.5 1,241,550 + Pf6N2E2_1064 0.39 +0.6 1,241,551 - Pf6N2E2_1064 0.39 -0.6 1,241,680 + Pf6N2E2_1064 0.49 +0.6
Or see this region's nucleotide sequence