Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5723 and Pf6N2E2_5724 are separated by 113 nucleotides Pf6N2E2_5724 and Pf6N2E2_5725 are separated by 26 nucleotides Pf6N2E2_5725 and Pf6N2E2_5726 are separated by 130 nucleotides
Pf6N2E2_5723 - GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2), at 6,539,922 to 6,542,168
_5723
Pf6N2E2_5724 - Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8), at 6,542,282 to 6,543,115
_5724
Pf6N2E2_5725 - nucleoprotein/polynucleotide-associated enzyme, at 6,543,142 to 6,543,681
_5725
Pf6N2E2_5726 - FIG00953102: hypothetical protein, at 6,543,812 to 6,544,006
_5726
Position (kb)
6542
6543
6544 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 6541.308 kb on + strand, within Pf6N2E2_5723 at 6541.309 kb on - strand, within Pf6N2E2_5723 at 6541.309 kb on - strand, within Pf6N2E2_5723 at 6541.396 kb on - strand, within Pf6N2E2_5723 at 6541.696 kb on - strand, within Pf6N2E2_5723 at 6541.800 kb on + strand, within Pf6N2E2_5723 at 6541.800 kb on + strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.801 kb on - strand, within Pf6N2E2_5723 at 6541.923 kb on + strand, within Pf6N2E2_5723 at 6542.167 kb on - strand at 6542.167 kb on - strand at 6542.260 kb on + strand at 6542.261 kb on - strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.285 kb on + strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.286 kb on - strand at 6542.336 kb on + strand at 6542.366 kb on + strand, within Pf6N2E2_5724 at 6542.366 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.386 kb on + strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.387 kb on - strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.402 kb on + strand, within Pf6N2E2_5724 at 6542.403 kb on - strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.483 kb on + strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.484 kb on - strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.486 kb on + strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.487 kb on - strand, within Pf6N2E2_5724 at 6542.598 kb on - strand, within Pf6N2E2_5724 at 6542.850 kb on - strand, within Pf6N2E2_5724 at 6542.999 kb on - strand, within Pf6N2E2_5724 at 6543.126 kb on + strand at 6543.126 kb on + strand at 6543.127 kb on - strand at 6543.430 kb on + strand, within Pf6N2E2_5725 at 6543.430 kb on + strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.434 kb on - strand, within Pf6N2E2_5725 at 6543.652 kb on + strand at 6543.762 kb on + strand at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.874 kb on + strand, within Pf6N2E2_5726 at 6543.874 kb on + strand, within Pf6N2E2_5726 at 6543.875 kb on - strand, within Pf6N2E2_5726 at 6543.875 kb on - strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.973 kb on - strand, within Pf6N2E2_5726 at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.029 kb on - strand at 6544.029 kb on - strand at 6544.062 kb on - strand at 6544.080 kb on - strand at 6544.083 kb on + strand at 6544.084 kb on - strand at 6544.084 kb on - strand at 6544.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 6,541,308 + Pf6N2E2_5723 0.62 -0.1 6,541,309 - Pf6N2E2_5723 0.62 -1.4 6,541,309 - Pf6N2E2_5723 0.62 -2.4 6,541,396 - Pf6N2E2_5723 0.66 -1.1 6,541,696 - Pf6N2E2_5723 0.79 -2.8 6,541,800 + Pf6N2E2_5723 0.84 -3.1 6,541,800 + Pf6N2E2_5723 0.84 -4.3 6,541,801 - Pf6N2E2_5723 0.84 -2.7 6,541,801 - Pf6N2E2_5723 0.84 -2.2 6,541,801 - Pf6N2E2_5723 0.84 -3.6 6,541,923 + Pf6N2E2_5723 0.89 -2.9 6,542,167 - -0.1 6,542,167 - -0.5 6,542,260 + +0.2 6,542,261 - +0.4 6,542,285 + +0.6 6,542,285 + +0.5 6,542,285 + +0.1 6,542,285 + +0.7 6,542,285 + -0.7 6,542,286 - -0.6 6,542,286 - +0.1 6,542,286 - +0.1 6,542,286 - +0.4 6,542,286 - -1.2 6,542,286 - -0.4 6,542,286 - -0.8 6,542,336 + -0.4 6,542,366 + Pf6N2E2_5724 0.10 -1.7 6,542,366 + Pf6N2E2_5724 0.10 -1.1 6,542,386 + Pf6N2E2_5724 0.12 -2.3 6,542,386 + Pf6N2E2_5724 0.12 +0.8 6,542,386 + Pf6N2E2_5724 0.12 -1.1 6,542,387 - Pf6N2E2_5724 0.13 +0.8 6,542,387 - Pf6N2E2_5724 0.13 -2.3 6,542,387 - Pf6N2E2_5724 0.13 -0.4 6,542,387 - Pf6N2E2_5724 0.13 +0.9 6,542,402 + Pf6N2E2_5724 0.14 -0.4 6,542,402 + Pf6N2E2_5724 0.14 -0.9 6,542,402 + Pf6N2E2_5724 0.14 -0.3 6,542,403 - Pf6N2E2_5724 0.15 +0.5 6,542,483 + Pf6N2E2_5724 0.24 +0.2 6,542,483 + Pf6N2E2_5724 0.24 -0.3 6,542,483 + Pf6N2E2_5724 0.24 -0.5 6,542,483 + Pf6N2E2_5724 0.24 -1.2 6,542,483 + Pf6N2E2_5724 0.24 -0.1 6,542,484 - Pf6N2E2_5724 0.24 -0.0 6,542,484 - Pf6N2E2_5724 0.24 +0.5 6,542,484 - Pf6N2E2_5724 0.24 -2.6 6,542,486 + Pf6N2E2_5724 0.24 -1.1 6,542,486 + Pf6N2E2_5724 0.24 -0.4 6,542,486 + Pf6N2E2_5724 0.24 -1.4 6,542,486 + Pf6N2E2_5724 0.24 -2.1 6,542,487 - Pf6N2E2_5724 0.25 +2.5 6,542,487 - Pf6N2E2_5724 0.25 -1.4 6,542,487 - Pf6N2E2_5724 0.25 -0.2 6,542,487 - Pf6N2E2_5724 0.25 -1.1 6,542,487 - Pf6N2E2_5724 0.25 +0.1 6,542,487 - Pf6N2E2_5724 0.25 -0.5 6,542,487 - Pf6N2E2_5724 0.25 -0.3 6,542,598 - Pf6N2E2_5724 0.38 +1.6 6,542,850 - Pf6N2E2_5724 0.68 -3.0 6,542,999 - Pf6N2E2_5724 0.86 +0.7 6,543,126 + -0.1 6,543,126 + -2.0 6,543,127 - -0.1 6,543,430 + Pf6N2E2_5725 0.53 +0.7 6,543,430 + Pf6N2E2_5725 0.53 -1.1 6,543,431 - Pf6N2E2_5725 0.54 -3.3 6,543,431 - Pf6N2E2_5725 0.54 +0.6 6,543,431 - Pf6N2E2_5725 0.54 +0.0 6,543,433 + Pf6N2E2_5725 0.54 -0.6 6,543,433 + Pf6N2E2_5725 0.54 +0.6 6,543,433 + Pf6N2E2_5725 0.54 -1.1 6,543,433 + Pf6N2E2_5725 0.54 -0.8 6,543,433 + Pf6N2E2_5725 0.54 +0.9 6,543,433 + Pf6N2E2_5725 0.54 +0.6 6,543,433 + Pf6N2E2_5725 0.54 -0.4 6,543,434 - Pf6N2E2_5725 0.54 -1.0 6,543,652 + -0.1 6,543,762 + -0.6 6,543,870 + Pf6N2E2_5726 0.30 -0.4 6,543,870 + Pf6N2E2_5726 0.30 -2.8 6,543,870 + Pf6N2E2_5726 0.30 +0.8 6,543,870 + Pf6N2E2_5726 0.30 +0.1 6,543,870 + Pf6N2E2_5726 0.30 -0.5 6,543,870 + Pf6N2E2_5726 0.30 -0.2 6,543,870 + Pf6N2E2_5726 0.30 +0.3 6,543,870 + Pf6N2E2_5726 0.30 +0.9 6,543,871 - Pf6N2E2_5726 0.30 -0.4 6,543,871 - Pf6N2E2_5726 0.30 -0.2 6,543,871 - Pf6N2E2_5726 0.30 +1.4 6,543,871 - Pf6N2E2_5726 0.30 -0.5 6,543,871 - Pf6N2E2_5726 0.30 +0.6 6,543,871 - Pf6N2E2_5726 0.30 -0.3 6,543,871 - Pf6N2E2_5726 0.30 +0.3 6,543,871 - Pf6N2E2_5726 0.30 -1.6 6,543,871 - Pf6N2E2_5726 0.30 +1.5 6,543,871 - Pf6N2E2_5726 0.30 +0.0 6,543,871 - Pf6N2E2_5726 0.30 +0.1 6,543,874 + Pf6N2E2_5726 0.32 +0.5 6,543,874 + Pf6N2E2_5726 0.32 -1.4 6,543,875 - Pf6N2E2_5726 0.32 -0.9 6,543,875 - Pf6N2E2_5726 0.32 +0.7 6,543,970 + Pf6N2E2_5726 0.81 +0.9 6,543,970 + Pf6N2E2_5726 0.81 -0.6 6,543,970 + Pf6N2E2_5726 0.81 +0.6 6,543,970 + Pf6N2E2_5726 0.81 +1.3 6,543,970 + Pf6N2E2_5726 0.81 -0.4 6,543,970 + Pf6N2E2_5726 0.81 -1.2 6,543,970 + Pf6N2E2_5726 0.81 +0.0 6,543,970 + Pf6N2E2_5726 0.81 -1.9 6,543,970 + Pf6N2E2_5726 0.81 +0.1 6,543,970 + Pf6N2E2_5726 0.81 -1.1 6,543,970 + Pf6N2E2_5726 0.81 -0.3 6,543,970 + Pf6N2E2_5726 0.81 +0.4 6,543,971 - Pf6N2E2_5726 0.82 -0.1 6,543,971 - Pf6N2E2_5726 0.82 +0.4 6,543,971 - Pf6N2E2_5726 0.82 +1.5 6,543,971 - Pf6N2E2_5726 0.82 -1.3 6,543,971 - Pf6N2E2_5726 0.82 -0.1 6,543,973 - Pf6N2E2_5726 0.83 -1.9 6,543,993 - +0.9 6,543,993 - +0.5 6,543,993 - -0.2 6,543,993 - -2.4 6,543,993 - -0.8 6,544,028 + +1.3 6,544,028 + -0.7 6,544,028 + +0.5 6,544,028 + -1.4 6,544,029 - +0.9 6,544,029 - +1.5 6,544,062 - +2.5 6,544,080 - -1.1 6,544,083 + -0.2 6,544,084 - +0.2 6,544,084 - +0.9 6,544,084 - -0.6
Or see this region's nucleotide sequence