Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2880 and Pf6N2E2_2881 are separated by 149 nucleotides Pf6N2E2_2881 and Pf6N2E2_2882 are separated by 17 nucleotides Pf6N2E2_2882 and Pf6N2E2_2883 are separated by 233 nucleotides Pf6N2E2_2883 and Pf6N2E2_2884 are separated by 17 nucleotides
Pf6N2E2_2880 - FIG00959696: hypothetical protein, at 3,368,589 to 3,369,053
_2880
Pf6N2E2_2881 - Putative cytoplasmic protein, at 3,369,203 to 3,369,496
_2881
Pf6N2E2_2882 - Isopenicillin N epimerase (EC 5.1.1.17), at 3,369,514 to 3,370,701
_2882
Pf6N2E2_2883 - 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16) @ 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14), at 3,370,935 to 3,371,600
_2883
Pf6N2E2_2884 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 3,371,618 to 3,372,331
_2884
Position (kb)
3369
3370
3371 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.590 kb on + strand at 3368.591 kb on - strand at 3368.609 kb on + strand at 3368.654 kb on - strand, within Pf6N2E2_2880 at 3368.679 kb on - strand, within Pf6N2E2_2880 at 3368.722 kb on + strand, within Pf6N2E2_2880 at 3368.875 kb on - strand, within Pf6N2E2_2880 at 3369.227 kb on - strand at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.314 kb on + strand, within Pf6N2E2_2881 at 3369.315 kb on - strand, within Pf6N2E2_2881 at 3369.315 kb on - strand, within Pf6N2E2_2881 at 3369.531 kb on - strand at 3369.575 kb on - strand at 3369.627 kb on + strand at 3369.627 kb on + strand at 3369.627 kb on + strand at 3369.628 kb on - strand at 3369.628 kb on - strand at 3369.628 kb on - strand at 3369.652 kb on + strand, within Pf6N2E2_2882 at 3369.653 kb on - strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.694 kb on + strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.695 kb on - strand, within Pf6N2E2_2882 at 3369.829 kb on + strand, within Pf6N2E2_2882 at 3369.829 kb on + strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.830 kb on - strand, within Pf6N2E2_2882 at 3369.878 kb on - strand, within Pf6N2E2_2882 at 3369.878 kb on - strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.036 kb on + strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.037 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.235 kb on - strand, within Pf6N2E2_2882 at 3370.288 kb on + strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.289 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.421 kb on - strand, within Pf6N2E2_2882 at 3370.498 kb on + strand, within Pf6N2E2_2882 at 3370.498 kb on + strand, within Pf6N2E2_2882 at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.699 kb on + strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.700 kb on - strand at 3370.852 kb on + strand at 3370.852 kb on + strand at 3370.853 kb on - strand at 3370.853 kb on - strand at 3370.898 kb on - strand at 3370.905 kb on + strand at 3370.906 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.632 kb on - strand at 3371.645 kb on - strand at 3371.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 3,368,590 + -1.6 3,368,590 + +0.6 3,368,590 + -1.3 3,368,590 + +0.8 3,368,590 + -0.4 3,368,591 - -0.4 3,368,609 + +1.0 3,368,654 - Pf6N2E2_2880 0.14 -1.2 3,368,679 - Pf6N2E2_2880 0.19 -1.0 3,368,722 + Pf6N2E2_2880 0.29 +1.0 3,368,875 - Pf6N2E2_2880 0.62 -1.8 3,369,227 - +1.0 3,369,314 + Pf6N2E2_2881 0.38 -0.8 3,369,314 + Pf6N2E2_2881 0.38 +1.4 3,369,314 + Pf6N2E2_2881 0.38 +1.2 3,369,314 + Pf6N2E2_2881 0.38 +0.1 3,369,315 - Pf6N2E2_2881 0.38 -1.2 3,369,315 - Pf6N2E2_2881 0.38 +0.5 3,369,531 - +0.8 3,369,575 - +1.4 3,369,627 + +0.1 3,369,627 + +0.2 3,369,627 + +1.6 3,369,628 - +0.0 3,369,628 - +0.8 3,369,628 - +1.6 3,369,652 + Pf6N2E2_2882 0.12 +0.9 3,369,653 - Pf6N2E2_2882 0.12 -1.3 3,369,694 + Pf6N2E2_2882 0.15 +0.6 3,369,694 + Pf6N2E2_2882 0.15 +0.1 3,369,694 + Pf6N2E2_2882 0.15 +0.4 3,369,695 - Pf6N2E2_2882 0.15 +0.7 3,369,695 - Pf6N2E2_2882 0.15 -1.3 3,369,695 - Pf6N2E2_2882 0.15 -1.0 3,369,829 + Pf6N2E2_2882 0.27 +1.1 3,369,829 + Pf6N2E2_2882 0.27 +1.8 3,369,830 - Pf6N2E2_2882 0.27 -0.7 3,369,830 - Pf6N2E2_2882 0.27 +0.9 3,369,830 - Pf6N2E2_2882 0.27 +0.0 3,369,830 - Pf6N2E2_2882 0.27 -0.6 3,369,830 - Pf6N2E2_2882 0.27 -0.2 3,369,830 - Pf6N2E2_2882 0.27 +0.8 3,369,830 - Pf6N2E2_2882 0.27 -1.0 3,369,878 - Pf6N2E2_2882 0.31 +1.0 3,369,878 - Pf6N2E2_2882 0.31 -0.2 3,370,036 + Pf6N2E2_2882 0.44 -1.0 3,370,036 + Pf6N2E2_2882 0.44 -0.5 3,370,036 + Pf6N2E2_2882 0.44 -0.6 3,370,036 + Pf6N2E2_2882 0.44 +1.3 3,370,037 - Pf6N2E2_2882 0.44 -2.3 3,370,037 - Pf6N2E2_2882 0.44 +0.1 3,370,235 - Pf6N2E2_2882 0.61 -2.5 3,370,235 - Pf6N2E2_2882 0.61 -0.2 3,370,288 + Pf6N2E2_2882 0.65 +1.3 3,370,289 - Pf6N2E2_2882 0.65 -0.6 3,370,289 - Pf6N2E2_2882 0.65 -1.3 3,370,421 - Pf6N2E2_2882 0.76 +1.8 3,370,421 - Pf6N2E2_2882 0.76 -0.7 3,370,421 - Pf6N2E2_2882 0.76 -2.2 3,370,498 + Pf6N2E2_2882 0.83 -3.1 3,370,498 + Pf6N2E2_2882 0.83 -0.2 3,370,699 + -0.3 3,370,699 + +1.4 3,370,699 + +1.4 3,370,699 + -0.2 3,370,699 + +0.2 3,370,699 + -0.2 3,370,699 + -0.7 3,370,699 + -0.0 3,370,699 + +2.1 3,370,699 + -0.8 3,370,700 - -0.1 3,370,700 - -0.0 3,370,700 - -0.7 3,370,700 - -0.6 3,370,700 - -0.0 3,370,700 - +0.2 3,370,700 - -0.0 3,370,700 - +0.6 3,370,700 - +0.6 3,370,700 - +0.1 3,370,700 - -0.6 3,370,700 - -1.0 3,370,700 - -0.0 3,370,700 - -0.8 3,370,852 + -0.0 3,370,852 + +0.5 3,370,853 - +0.4 3,370,853 - -1.6 3,370,898 - +0.1 3,370,905 + +0.7 3,370,906 - +1.1 3,371,632 - -1.1 3,371,632 - -1.3 3,371,632 - -1.9 3,371,645 - -0.2 3,371,645 - +0.5
Or see this region's nucleotide sequence