Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1443 and Pf6N2E2_1444 are separated by 92 nucleotides Pf6N2E2_1444 and Pf6N2E2_1445 are separated by 133 nucleotides Pf6N2E2_1445 and Pf6N2E2_1446 are separated by 238 nucleotides
Pf6N2E2_1443 - putative aldo/keto reductase, at 1,693,084 to 1,694,091
_1443
Pf6N2E2_1444 - Transcriptional regulator, LysR family, at 1,694,184 to 1,695,086
_1444
Pf6N2E2_1445 - Glutathione S-transferase, at 1,695,220 to 1,695,831
_1445
Pf6N2E2_1446 - Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10), at 1,696,070 to 1,696,822
_1446
Position (kb)
1694
1695
1696 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.527 kb on - strand, within Pf6N2E2_1443 at 1693.573 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1694.091 kb on + strand at 1694.092 kb on - strand at 1694.386 kb on + strand, within Pf6N2E2_1444 at 1694.706 kb on + strand, within Pf6N2E2_1444 at 1694.706 kb on + strand, within Pf6N2E2_1444 at 1694.707 kb on - strand, within Pf6N2E2_1444 at 1694.805 kb on + strand, within Pf6N2E2_1444 at 1694.805 kb on + strand, within Pf6N2E2_1444 at 1694.806 kb on - strand, within Pf6N2E2_1444 at 1695.006 kb on + strand at 1695.007 kb on - strand at 1695.007 kb on - strand at 1695.009 kb on + strand at 1695.010 kb on - strand at 1695.133 kb on + strand at 1695.133 kb on + strand at 1695.133 kb on + strand at 1695.134 kb on - strand at 1695.417 kb on - strand, within Pf6N2E2_1445 at 1695.470 kb on - strand, within Pf6N2E2_1445 at 1695.617 kb on - strand, within Pf6N2E2_1445 at 1695.691 kb on + strand, within Pf6N2E2_1445 at 1695.785 kb on - strand at 1695.796 kb on + strand at 1695.796 kb on + strand at 1695.797 kb on - strand at 1695.847 kb on + strand at 1695.860 kb on - strand at 1695.871 kb on + strand at 1695.879 kb on + strand at 1695.879 kb on + strand at 1695.879 kb on + strand at 1695.880 kb on - strand at 1695.898 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.917 kb on - strand at 1695.920 kb on - strand at 1695.920 kb on - strand at 1695.991 kb on - strand at 1695.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 1,693,452 - Pf6N2E2_1443 0.37 +1.3 1,693,452 - Pf6N2E2_1443 0.37 -1.0 1,693,527 - Pf6N2E2_1443 0.44 +1.6 1,693,573 - Pf6N2E2_1443 0.49 +0.3 1,693,911 - Pf6N2E2_1443 0.82 -0.8 1,693,911 - Pf6N2E2_1443 0.82 -0.6 1,693,955 + Pf6N2E2_1443 0.86 +2.4 1,693,955 + Pf6N2E2_1443 0.86 -1.4 1,693,955 + Pf6N2E2_1443 0.86 -1.0 1,694,091 + -0.7 1,694,092 - -1.3 1,694,386 + Pf6N2E2_1444 0.22 -0.7 1,694,706 + Pf6N2E2_1444 0.58 -1.7 1,694,706 + Pf6N2E2_1444 0.58 +0.3 1,694,707 - Pf6N2E2_1444 0.58 -0.3 1,694,805 + Pf6N2E2_1444 0.69 -0.7 1,694,805 + Pf6N2E2_1444 0.69 -2.5 1,694,806 - Pf6N2E2_1444 0.69 -2.1 1,695,006 + +0.9 1,695,007 - -1.5 1,695,007 - -0.6 1,695,009 + +0.8 1,695,010 - -0.7 1,695,133 + +0.6 1,695,133 + +1.1 1,695,133 + -0.3 1,695,134 - +1.1 1,695,417 - Pf6N2E2_1445 0.32 +0.3 1,695,470 - Pf6N2E2_1445 0.41 -0.7 1,695,617 - Pf6N2E2_1445 0.65 +0.3 1,695,691 + Pf6N2E2_1445 0.77 +0.5 1,695,785 - +0.3 1,695,796 + -0.7 1,695,796 + -0.7 1,695,797 - +0.5 1,695,847 + -0.3 1,695,860 - +0.0 1,695,871 + -1.3 1,695,879 + +0.3 1,695,879 + -0.4 1,695,879 + +1.1 1,695,880 - -1.0 1,695,898 + -0.4 1,695,916 + -2.5 1,695,916 + -0.2 1,695,916 + +1.3 1,695,916 + +0.3 1,695,916 + -2.3 1,695,917 - -0.7 1,695,920 - -0.7 1,695,920 - -0.7 1,695,991 - +0.3 1,695,991 - +1.4
Or see this region's nucleotide sequence