Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_872 and Pf6N2E2_873 are separated by 199 nucleotides Pf6N2E2_873 and Pf6N2E2_874 are separated by 115 nucleotides Pf6N2E2_874 and Pf6N2E2_875 are separated by 60 nucleotides
Pf6N2E2_872 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 1,020,896 to 1,022,863
_872
Pf6N2E2_873 - Methyl-accepting chemotaxis protein, at 1,023,063 to 1,024,352
_873
Pf6N2E2_874 - Predicted cobalt transporter CbtC, at 1,024,468 to 1,025,145
_874
Pf6N2E2_875 - putative metal chaperone, involved in Fe-nitrile hydratase activation, GTPase of COG0523 family, at 1,025,206 to 1,025,646
_875
Position (kb)
1023
1024
1025 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1022.212 kb on - strand, within Pf6N2E2_872 at 1022.266 kb on - strand, within Pf6N2E2_872 at 1022.317 kb on - strand, within Pf6N2E2_872 at 1022.544 kb on + strand, within Pf6N2E2_872 at 1022.544 kb on + strand, within Pf6N2E2_872 at 1022.875 kb on + strand at 1022.875 kb on + strand at 1022.875 kb on + strand at 1022.876 kb on - strand at 1022.876 kb on - strand at 1022.876 kb on - strand at 1022.970 kb on + strand at 1022.971 kb on - strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.041 kb on - strand at 1023.041 kb on - strand at 1023.041 kb on - strand at 1023.067 kb on + strand at 1023.067 kb on + strand at 1023.068 kb on - strand at 1023.186 kb on + strand at 1023.186 kb on + strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.207 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.360 kb on - strand, within Pf6N2E2_873 at 1023.360 kb on - strand, within Pf6N2E2_873 at 1023.418 kb on + strand, within Pf6N2E2_873 at 1023.419 kb on - strand, within Pf6N2E2_873 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873 at 1023.969 kb on + strand, within Pf6N2E2_873 at 1023.970 kb on - strand, within Pf6N2E2_873 at 1023.973 kb on + strand, within Pf6N2E2_873 at 1023.973 kb on + strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand, within Pf6N2E2_873 at 1023.974 kb on - strand at 1024.020 kb on + strand, within Pf6N2E2_873 at 1024.021 kb on - strand, within Pf6N2E2_873 at 1024.021 kb on - strand, within Pf6N2E2_873 at 1024.275 kb on + strand at 1024.275 kb on + strand at 1024.276 kb on - strand at 1024.276 kb on - strand at 1024.337 kb on + strand at 1024.338 kb on - strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.357 kb on + strand at 1024.482 kb on - strand at 1024.586 kb on + strand, within Pf6N2E2_874 at 1024.586 kb on + strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.587 kb on - strand, within Pf6N2E2_874 at 1024.643 kb on + strand, within Pf6N2E2_874 at 1024.644 kb on - strand, within Pf6N2E2_874 at 1024.713 kb on + strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.714 kb on - strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.715 kb on + strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.716 kb on - strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.730 kb on + strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.731 kb on - strand, within Pf6N2E2_874 at 1024.814 kb on + strand, within Pf6N2E2_874 at 1024.815 kb on - strand, within Pf6N2E2_874 at 1024.963 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.984 kb on + strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1024.985 kb on - strand, within Pf6N2E2_874 at 1025.069 kb on + strand, within Pf6N2E2_874 at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.096 kb on + strand at 1025.097 kb on - strand at 1025.097 kb on - strand at 1025.130 kb on + strand at 1025.322 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.330 kb on + strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875 at 1025.331 kb on - strand, within Pf6N2E2_875
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,022,212 - Pf6N2E2_872 0.67 -2.2 1,022,266 - Pf6N2E2_872 0.70 +0.7 1,022,317 - Pf6N2E2_872 0.72 -0.4 1,022,544 + Pf6N2E2_872 0.84 +0.6 1,022,544 + Pf6N2E2_872 0.84 -0.0 1,022,875 + -0.1 1,022,875 + -2.8 1,022,875 + +1.0 1,022,876 - +0.3 1,022,876 - -1.3 1,022,876 - +0.8 1,022,970 + -0.4 1,022,971 - +0.6 1,023,040 + -0.8 1,023,040 + +0.2 1,023,040 + -1.8 1,023,040 + +0.1 1,023,041 - -1.2 1,023,041 - -0.5 1,023,041 - +1.1 1,023,067 + +0.3 1,023,067 + +0.3 1,023,068 - -2.0 1,023,186 + -1.2 1,023,186 + -2.0 1,023,187 - -0.0 1,023,187 - +2.6 1,023,187 - +2.6 1,023,187 - -2.0 1,023,207 + Pf6N2E2_873 0.11 -1.2 1,023,359 + Pf6N2E2_873 0.23 +1.9 1,023,359 + Pf6N2E2_873 0.23 -2.5 1,023,359 + Pf6N2E2_873 0.23 -1.1 1,023,359 + Pf6N2E2_873 0.23 +0.6 1,023,360 - Pf6N2E2_873 0.23 -2.2 1,023,360 - Pf6N2E2_873 0.23 +1.6 1,023,418 + Pf6N2E2_873 0.28 +0.6 1,023,419 - Pf6N2E2_873 0.28 -0.4 1,023,656 + Pf6N2E2_873 0.46 -2.2 1,023,656 + Pf6N2E2_873 0.46 +1.0 1,023,657 - Pf6N2E2_873 0.46 +0.6 1,023,657 - Pf6N2E2_873 0.46 +0.7 1,023,657 - Pf6N2E2_873 0.46 +0.6 1,023,657 - Pf6N2E2_873 0.46 -0.8 1,023,835 + Pf6N2E2_873 0.60 +0.6 1,023,835 + Pf6N2E2_873 0.60 -0.7 1,023,969 + Pf6N2E2_873 0.70 +0.6 1,023,970 - Pf6N2E2_873 0.70 -0.9 1,023,973 + Pf6N2E2_873 0.71 -0.6 1,023,973 + Pf6N2E2_873 0.71 -0.4 1,023,974 - Pf6N2E2_873 0.71 -0.1 1,023,974 - Pf6N2E2_873 0.71 -0.8 1,023,974 - Pf6N2E2_873 0.71 +0.6 1,023,974 - Pf6N2E2_873 0.71 -0.4 1,023,974 - Pf6N2E2_873 0.71 -1.8 1,023,974 - Pf6N2E2_873 0.71 -0.3 1,023,974 - +0.2 1,024,020 + Pf6N2E2_873 0.74 -0.4 1,024,021 - Pf6N2E2_873 0.74 -1.0 1,024,021 - Pf6N2E2_873 0.74 -0.8 1,024,275 + -0.3 1,024,275 + +0.2 1,024,276 - +0.6 1,024,276 - +1.3 1,024,337 + +0.3 1,024,338 - +3.2 1,024,357 + -0.1 1,024,357 + +1.1 1,024,357 + +1.3 1,024,357 + -1.8 1,024,482 - +0.5 1,024,586 + Pf6N2E2_874 0.17 +1.0 1,024,586 + Pf6N2E2_874 0.17 -1.0 1,024,587 - Pf6N2E2_874 0.18 +0.8 1,024,587 - Pf6N2E2_874 0.18 -0.6 1,024,587 - Pf6N2E2_874 0.18 +0.2 1,024,587 - Pf6N2E2_874 0.18 -2.2 1,024,587 - Pf6N2E2_874 0.18 +1.3 1,024,643 + Pf6N2E2_874 0.26 +0.6 1,024,644 - Pf6N2E2_874 0.26 -0.4 1,024,713 + Pf6N2E2_874 0.36 -0.4 1,024,714 - Pf6N2E2_874 0.36 +1.6 1,024,714 - Pf6N2E2_874 0.36 -2.6 1,024,715 + Pf6N2E2_874 0.36 +0.6 1,024,715 + Pf6N2E2_874 0.36 +0.7 1,024,715 + Pf6N2E2_874 0.36 +0.6 1,024,716 - Pf6N2E2_874 0.37 +0.6 1,024,716 - Pf6N2E2_874 0.37 -1.1 1,024,716 - Pf6N2E2_874 0.37 -1.4 1,024,730 + Pf6N2E2_874 0.39 -1.0 1,024,730 + Pf6N2E2_874 0.39 -0.4 1,024,730 + Pf6N2E2_874 0.39 -1.4 1,024,730 + Pf6N2E2_874 0.39 +1.0 1,024,730 + Pf6N2E2_874 0.39 +1.0 1,024,730 + Pf6N2E2_874 0.39 +0.7 1,024,731 - Pf6N2E2_874 0.39 +0.4 1,024,731 - Pf6N2E2_874 0.39 +0.6 1,024,814 + Pf6N2E2_874 0.51 -0.4 1,024,815 - Pf6N2E2_874 0.51 -0.2 1,024,963 + Pf6N2E2_874 0.73 +1.9 1,024,984 + Pf6N2E2_874 0.76 +0.0 1,024,984 + Pf6N2E2_874 0.76 +0.6 1,024,985 - Pf6N2E2_874 0.76 -2.8 1,024,985 - Pf6N2E2_874 0.76 +0.6 1,025,069 + Pf6N2E2_874 0.89 +0.8 1,025,096 + -0.8 1,025,096 + +0.1 1,025,096 + +1.4 1,025,096 + -0.4 1,025,096 + +0.4 1,025,096 + +0.6 1,025,097 - +0.7 1,025,097 - -0.3 1,025,130 + -2.0 1,025,322 + Pf6N2E2_875 0.26 +0.6 1,025,330 + Pf6N2E2_875 0.28 -1.4 1,025,330 + Pf6N2E2_875 0.28 +0.6 1,025,331 - Pf6N2E2_875 0.28 +0.4 1,025,331 - Pf6N2E2_875 0.28 +1.1 1,025,331 - Pf6N2E2_875 0.28 -3.0 1,025,331 - Pf6N2E2_875 0.28 -0.3
Or see this region's nucleotide sequence