Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_758 and Pf6N2E2_759 are separated by 8 nucleotides Pf6N2E2_759 and Pf6N2E2_760 are separated by 163 nucleotides Pf6N2E2_760 and Pf6N2E2_761 are separated by 95 nucleotides
Pf6N2E2_758 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 867,937 to 869,457
_758
Pf6N2E2_759 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 869,466 to 870,275
_759
Pf6N2E2_760 - Na+/H+ antiporter NhaB, at 870,439 to 871,941
_760
Pf6N2E2_761 - Hemolysin-type calcium-binding region, at 872,037 to 873,326
_761
Position (kb)
870
871
872 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 869.508 kb on - strand at 869.718 kb on + strand, within Pf6N2E2_759 at 869.719 kb on - strand, within Pf6N2E2_759 at 869.719 kb on - strand, within Pf6N2E2_759 at 869.788 kb on + strand, within Pf6N2E2_759 at 869.788 kb on + strand, within Pf6N2E2_759 at 869.789 kb on - strand, within Pf6N2E2_759 at 869.789 kb on - strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.895 kb on + strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 869.896 kb on - strand, within Pf6N2E2_759 at 870.018 kb on + strand, within Pf6N2E2_759 at 870.258 kb on + strand at 870.258 kb on + strand at 870.335 kb on - strand at 870.335 kb on - strand at 870.335 kb on - strand at 870.434 kb on + strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.435 kb on - strand at 870.440 kb on + strand at 870.440 kb on + strand at 870.440 kb on + strand at 870.441 kb on - strand at 870.449 kb on + strand at 870.450 kb on - strand at 870.450 kb on - strand at 870.450 kb on - strand at 870.462 kb on - strand at 870.492 kb on - strand at 870.522 kb on - strand at 870.693 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.698 kb on + strand, within Pf6N2E2_760 at 870.699 kb on - strand, within Pf6N2E2_760 at 871.023 kb on + strand, within Pf6N2E2_760 at 871.024 kb on - strand, within Pf6N2E2_760 at 871.024 kb on - strand, within Pf6N2E2_760 at 871.085 kb on + strand, within Pf6N2E2_760 at 871.085 kb on + strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.106 kb on - strand, within Pf6N2E2_760 at 871.336 kb on + strand, within Pf6N2E2_760 at 871.337 kb on - strand, within Pf6N2E2_760 at 871.446 kb on - strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.454 kb on + strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.455 kb on - strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.619 kb on + strand, within Pf6N2E2_760 at 871.620 kb on - strand, within Pf6N2E2_760 at 871.620 kb on - strand, within Pf6N2E2_760 at 871.682 kb on + strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.683 kb on - strand, within Pf6N2E2_760 at 871.761 kb on + strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.762 kb on - strand, within Pf6N2E2_760 at 871.880 kb on + strand at 871.880 kb on + strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.881 kb on - strand at 871.908 kb on + strand at 871.908 kb on + strand at 871.955 kb on + strand at 871.993 kb on + strand at 872.008 kb on + strand at 872.008 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.012 kb on + strand at 872.120 kb on + strand at 872.214 kb on + strand, within Pf6N2E2_761 at 872.215 kb on - strand, within Pf6N2E2_761 at 872.406 kb on - strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.606 kb on + strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.607 kb on - strand, within Pf6N2E2_761 at 872.646 kb on - strand, within Pf6N2E2_761 at 872.646 kb on - strand, within Pf6N2E2_761 at 872.756 kb on - strand, within Pf6N2E2_761 at 872.796 kb on + strand, within Pf6N2E2_761
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 869,508 - +1.9 869,718 + Pf6N2E2_759 0.31 -0.1 869,719 - Pf6N2E2_759 0.31 -2.4 869,719 - Pf6N2E2_759 0.31 -0.4 869,788 + Pf6N2E2_759 0.40 -1.8 869,788 + Pf6N2E2_759 0.40 -0.4 869,789 - Pf6N2E2_759 0.40 +0.6 869,789 - Pf6N2E2_759 0.40 +0.7 869,895 + Pf6N2E2_759 0.53 +0.8 869,895 + Pf6N2E2_759 0.53 -1.6 869,895 + Pf6N2E2_759 0.53 -0.7 869,895 + Pf6N2E2_759 0.53 -1.0 869,895 + Pf6N2E2_759 0.53 +1.0 869,896 - Pf6N2E2_759 0.53 +0.6 869,896 - Pf6N2E2_759 0.53 -2.8 869,896 - Pf6N2E2_759 0.53 -0.7 870,018 + Pf6N2E2_759 0.68 -0.1 870,258 + +0.2 870,258 + +1.0 870,335 - +0.6 870,335 - +1.7 870,335 - -0.4 870,434 + -0.4 870,435 - +0.3 870,435 - -2.6 870,435 - -1.2 870,435 - -0.1 870,435 - -1.4 870,440 + -0.4 870,440 + -0.2 870,440 + +0.1 870,441 - +0.2 870,449 + +1.2 870,450 - +0.7 870,450 - -3.6 870,450 - -0.4 870,462 - -1.4 870,492 - -3.1 870,522 - -1.8 870,693 + Pf6N2E2_760 0.17 -3.6 870,698 + Pf6N2E2_760 0.17 -2.0 870,698 + Pf6N2E2_760 0.17 -3.9 870,698 + Pf6N2E2_760 0.17 -1.8 870,698 + Pf6N2E2_760 0.17 -1.3 870,698 + Pf6N2E2_760 0.17 -1.7 870,698 + Pf6N2E2_760 0.17 -4.1 870,698 + Pf6N2E2_760 0.17 +0.6 870,698 + Pf6N2E2_760 0.17 -3.4 870,698 + Pf6N2E2_760 0.17 +1.6 870,699 - Pf6N2E2_760 0.17 -1.5 871,023 + Pf6N2E2_760 0.39 +0.6 871,024 - Pf6N2E2_760 0.39 -1.6 871,024 - Pf6N2E2_760 0.39 -2.0 871,085 + Pf6N2E2_760 0.43 -3.0 871,085 + Pf6N2E2_760 0.43 -0.8 871,106 - Pf6N2E2_760 0.44 -3.3 871,106 - Pf6N2E2_760 0.44 -1.8 871,106 - Pf6N2E2_760 0.44 +0.6 871,106 - Pf6N2E2_760 0.44 -1.0 871,106 - Pf6N2E2_760 0.44 -1.4 871,106 - Pf6N2E2_760 0.44 -1.9 871,106 - Pf6N2E2_760 0.44 -0.5 871,106 - Pf6N2E2_760 0.44 -0.4 871,106 - Pf6N2E2_760 0.44 -0.7 871,336 + Pf6N2E2_760 0.60 -3.1 871,337 - Pf6N2E2_760 0.60 -2.3 871,446 - Pf6N2E2_760 0.67 -0.2 871,454 + Pf6N2E2_760 0.68 -1.4 871,454 + Pf6N2E2_760 0.68 -1.2 871,454 + Pf6N2E2_760 0.68 -0.7 871,454 + Pf6N2E2_760 0.68 -1.6 871,455 - Pf6N2E2_760 0.68 -3.2 871,455 - Pf6N2E2_760 0.68 -0.8 871,455 - Pf6N2E2_760 0.68 -1.0 871,619 + Pf6N2E2_760 0.79 -1.9 871,619 + Pf6N2E2_760 0.79 -1.2 871,619 + Pf6N2E2_760 0.79 +1.6 871,620 - Pf6N2E2_760 0.79 -1.3 871,620 - Pf6N2E2_760 0.79 -1.8 871,682 + Pf6N2E2_760 0.83 -2.0 871,683 - Pf6N2E2_760 0.83 -4.1 871,683 - Pf6N2E2_760 0.83 -3.4 871,683 - Pf6N2E2_760 0.83 -1.9 871,683 - Pf6N2E2_760 0.83 -3.7 871,683 - Pf6N2E2_760 0.83 -0.7 871,683 - Pf6N2E2_760 0.83 -3.2 871,761 + Pf6N2E2_760 0.88 -2.9 871,762 - Pf6N2E2_760 0.88 -3.2 871,762 - Pf6N2E2_760 0.88 +0.4 871,762 - Pf6N2E2_760 0.88 -1.8 871,762 - Pf6N2E2_760 0.88 -0.9 871,762 - Pf6N2E2_760 0.88 -1.9 871,762 - Pf6N2E2_760 0.88 -1.4 871,880 + -1.6 871,880 + -0.4 871,881 - -1.4 871,881 - -0.4 871,881 - -0.9 871,881 - -4.5 871,908 + -1.6 871,908 + +0.6 871,955 + -2.8 871,993 + -1.0 872,008 + -2.0 872,008 + -2.0 872,012 + -0.3 872,012 + -0.4 872,012 + -1.8 872,012 + -1.0 872,120 + -1.6 872,214 + Pf6N2E2_761 0.14 -0.4 872,215 - Pf6N2E2_761 0.14 +0.2 872,406 - Pf6N2E2_761 0.29 +1.2 872,606 + Pf6N2E2_761 0.44 -2.0 872,606 + Pf6N2E2_761 0.44 +0.8 872,606 + Pf6N2E2_761 0.44 +0.6 872,606 + Pf6N2E2_761 0.44 +0.8 872,606 + Pf6N2E2_761 0.44 -1.0 872,606 + Pf6N2E2_761 0.44 -0.8 872,606 + Pf6N2E2_761 0.44 +1.5 872,607 - Pf6N2E2_761 0.44 +1.9 872,607 - Pf6N2E2_761 0.44 +0.1 872,607 - Pf6N2E2_761 0.44 +0.6 872,646 - Pf6N2E2_761 0.47 -1.0 872,646 - Pf6N2E2_761 0.47 +0.6 872,756 - Pf6N2E2_761 0.56 -3.3 872,796 + Pf6N2E2_761 0.59 +0.6
Or see this region's nucleotide sequence