Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6053 and Pf6N2E2_6054 are separated by 450 nucleotides Pf6N2E2_6054 and Pf6N2E2_6055 are separated by 174 nucleotides Pf6N2E2_6055 and Pf6N2E2_6056 are separated by 142 nucleotides
Pf6N2E2_6053 - Hydantoin permease, at 6,871,523 to 6,873,052
_6053
Pf6N2E2_6054 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) / Adenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.10), at 6,873,503 to 6,874,246
_6054
Pf6N2E2_6055 - Homoserine kinase (EC 2.7.1.39), at 6,874,421 to 6,875,038
_6055
Pf6N2E2_6056 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 6,875,181 to 6,876,524
_6056
Position (kb)
6873
6874
6875 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6872.616 kb on + strand, within Pf6N2E2_6053 at 6872.616 kb on + strand, within Pf6N2E2_6053 at 6872.617 kb on - strand, within Pf6N2E2_6053 at 6872.617 kb on - strand, within Pf6N2E2_6053 at 6872.743 kb on - strand, within Pf6N2E2_6053 at 6872.766 kb on + strand, within Pf6N2E2_6053 at 6872.767 kb on - strand, within Pf6N2E2_6053 at 6872.767 kb on - strand, within Pf6N2E2_6053 at 6872.829 kb on + strand, within Pf6N2E2_6053 at 6872.829 kb on + strand, within Pf6N2E2_6053 at 6872.830 kb on - strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.850 kb on + strand, within Pf6N2E2_6053 at 6872.851 kb on - strand, within Pf6N2E2_6053 at 6872.851 kb on - strand, within Pf6N2E2_6053 at 6872.851 kb on - strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.863 kb on + strand, within Pf6N2E2_6053 at 6872.864 kb on - strand, within Pf6N2E2_6053 at 6872.898 kb on + strand, within Pf6N2E2_6053 at 6873.032 kb on - strand at 6873.032 kb on - strand at 6873.046 kb on + strand at 6873.046 kb on + strand at 6873.047 kb on - strand at 6873.047 kb on - strand at 6873.266 kb on - strand at 6873.360 kb on + strand at 6873.744 kb on + strand, within Pf6N2E2_6054 at 6874.285 kb on + strand at 6874.286 kb on - strand at 6874.286 kb on - strand at 6874.286 kb on - strand at 6874.288 kb on - strand at 6874.301 kb on + strand at 6874.393 kb on + strand at 6874.394 kb on - strand at 6874.536 kb on - strand, within Pf6N2E2_6055 at 6874.701 kb on - strand, within Pf6N2E2_6055 at 6874.701 kb on - strand, within Pf6N2E2_6055 at 6874.850 kb on + strand, within Pf6N2E2_6055 at 6874.850 kb on + strand, within Pf6N2E2_6055 at 6874.851 kb on - strand, within Pf6N2E2_6055 at 6874.851 kb on - strand, within Pf6N2E2_6055 at 6874.853 kb on + strand, within Pf6N2E2_6055 at 6874.880 kb on + strand, within Pf6N2E2_6055 at 6874.880 kb on + strand, within Pf6N2E2_6055 at 6874.880 kb on + strand, within Pf6N2E2_6055 at 6874.880 kb on + strand, within Pf6N2E2_6055 at 6874.881 kb on - strand, within Pf6N2E2_6055 at 6874.978 kb on + strand at 6874.978 kb on + strand at 6874.979 kb on - strand at 6874.979 kb on - strand at 6875.006 kb on - strand at 6875.006 kb on - strand at 6875.036 kb on + strand at 6875.036 kb on + strand at 6875.037 kb on - strand at 6875.087 kb on + strand at 6875.087 kb on + strand at 6875.087 kb on + strand at 6875.087 kb on + strand at 6875.088 kb on - strand at 6875.088 kb on - strand at 6875.171 kb on + strand at 6875.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,872,616 + Pf6N2E2_6053 0.71 -1.4 6,872,616 + Pf6N2E2_6053 0.71 -1.4 6,872,617 - Pf6N2E2_6053 0.72 -0.6 6,872,617 - Pf6N2E2_6053 0.72 -3.6 6,872,743 - Pf6N2E2_6053 0.80 +0.4 6,872,766 + Pf6N2E2_6053 0.81 -0.5 6,872,767 - Pf6N2E2_6053 0.81 -2.5 6,872,767 - Pf6N2E2_6053 0.81 -1.4 6,872,829 + Pf6N2E2_6053 0.85 -0.0 6,872,829 + Pf6N2E2_6053 0.85 +1.0 6,872,830 - Pf6N2E2_6053 0.85 -2.5 6,872,850 + Pf6N2E2_6053 0.87 -0.0 6,872,850 + Pf6N2E2_6053 0.87 +0.1 6,872,850 + Pf6N2E2_6053 0.87 +0.4 6,872,850 + Pf6N2E2_6053 0.87 -1.8 6,872,850 + Pf6N2E2_6053 0.87 -1.2 6,872,850 + Pf6N2E2_6053 0.87 -2.5 6,872,851 - Pf6N2E2_6053 0.87 -0.1 6,872,851 - Pf6N2E2_6053 0.87 -0.3 6,872,851 - Pf6N2E2_6053 0.87 -0.3 6,872,863 + Pf6N2E2_6053 0.88 -0.2 6,872,863 + Pf6N2E2_6053 0.88 +0.4 6,872,863 + Pf6N2E2_6053 0.88 -0.6 6,872,863 + Pf6N2E2_6053 0.88 -2.1 6,872,863 + Pf6N2E2_6053 0.88 -0.6 6,872,863 + Pf6N2E2_6053 0.88 +0.3 6,872,864 - Pf6N2E2_6053 0.88 +0.0 6,872,898 + Pf6N2E2_6053 0.90 -0.8 6,873,032 - -0.0 6,873,032 - +0.7 6,873,046 + +1.8 6,873,046 + -0.5 6,873,047 - +0.6 6,873,047 - -1.4 6,873,266 - -1.0 6,873,360 + -0.0 6,873,744 + Pf6N2E2_6054 0.32 -1.4 6,874,285 + +1.0 6,874,286 - +1.0 6,874,286 - +0.4 6,874,286 - +0.8 6,874,288 - +0.7 6,874,301 + -1.8 6,874,393 + -2.2 6,874,394 - +0.2 6,874,536 - Pf6N2E2_6055 0.19 +0.0 6,874,701 - Pf6N2E2_6055 0.45 +1.0 6,874,701 - Pf6N2E2_6055 0.45 -0.0 6,874,850 + Pf6N2E2_6055 0.69 -1.4 6,874,850 + Pf6N2E2_6055 0.69 +0.6 6,874,851 - Pf6N2E2_6055 0.70 +0.3 6,874,851 - Pf6N2E2_6055 0.70 -0.0 6,874,853 + Pf6N2E2_6055 0.70 +1.5 6,874,880 + Pf6N2E2_6055 0.74 -1.1 6,874,880 + Pf6N2E2_6055 0.74 +0.9 6,874,880 + Pf6N2E2_6055 0.74 +0.2 6,874,880 + Pf6N2E2_6055 0.74 -1.4 6,874,881 - Pf6N2E2_6055 0.74 -1.4 6,874,978 + +0.1 6,874,978 + -1.2 6,874,979 - +1.5 6,874,979 - -1.0 6,875,006 - -2.5 6,875,006 - +1.0 6,875,036 + -1.0 6,875,036 + +1.5 6,875,037 - -0.5 6,875,087 + +0.9 6,875,087 + +0.0 6,875,087 + +1.2 6,875,087 + +0.2 6,875,088 - -1.8 6,875,088 - +1.0 6,875,171 + +0.1 6,875,172 - -0.1
Or see this region's nucleotide sequence