Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5958 and Pf6N2E2_5959 are separated by 125 nucleotides Pf6N2E2_5959 and Pf6N2E2_5960 are separated by 11 nucleotides Pf6N2E2_5960 and Pf6N2E2_5961 are separated by 383 nucleotides
Pf6N2E2_5958 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 6,777,043 to 6,778,029
_5958
Pf6N2E2_5959 - Type IV pilus biogenesis protein PilZ, at 6,778,155 to 6,778,511
_5959
Pf6N2E2_5960 - Putative deoxyribonuclease YcfH, at 6,778,523 to 6,779,317
_5960
Pf6N2E2_5961 - Transcriptional regulator, TetR family, at 6,779,701 to 6,780,339
_5961
Position (kb)
6778
6779
6780 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6778.060 kb on + strand at 6778.061 kb on - strand at 6778.105 kb on + strand at 6778.105 kb on + strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.106 kb on - strand at 6778.124 kb on + strand at 6778.205 kb on + strand, within Pf6N2E2_5959 at 6778.228 kb on - strand, within Pf6N2E2_5959 at 6778.236 kb on + strand, within Pf6N2E2_5959 at 6778.236 kb on + strand, within Pf6N2E2_5959 at 6778.236 kb on + strand, within Pf6N2E2_5959 at 6778.236 kb on + strand, within Pf6N2E2_5959 at 6778.237 kb on - strand, within Pf6N2E2_5959 at 6778.237 kb on - strand, within Pf6N2E2_5959 at 6778.237 kb on - strand, within Pf6N2E2_5959 at 6778.237 kb on - strand, within Pf6N2E2_5959 at 6778.287 kb on + strand, within Pf6N2E2_5959 at 6778.287 kb on + strand, within Pf6N2E2_5959 at 6778.287 kb on + strand, within Pf6N2E2_5959 at 6778.288 kb on - strand, within Pf6N2E2_5959 at 6778.288 kb on - strand, within Pf6N2E2_5959 at 6778.502 kb on + strand at 6778.502 kb on + strand at 6778.502 kb on + strand at 6778.502 kb on + strand at 6778.502 kb on + strand at 6778.503 kb on - strand at 6778.503 kb on - strand at 6778.509 kb on + strand at 6778.510 kb on - strand at 6778.522 kb on + strand at 6778.522 kb on + strand at 6778.522 kb on + strand at 6778.522 kb on + strand at 6778.522 kb on + strand at 6778.522 kb on + strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.523 kb on - strand at 6778.530 kb on + strand at 6778.530 kb on + strand at 6778.530 kb on + strand at 6778.530 kb on + strand at 6778.531 kb on - strand at 6778.531 kb on - strand at 6778.531 kb on - strand at 6778.531 kb on - strand at 6778.820 kb on + strand, within Pf6N2E2_5960 at 6778.820 kb on + strand, within Pf6N2E2_5960 at 6778.820 kb on + strand, within Pf6N2E2_5960 at 6778.820 kb on + strand, within Pf6N2E2_5960 at 6778.821 kb on - strand, within Pf6N2E2_5960 at 6778.827 kb on - strand, within Pf6N2E2_5960 at 6778.827 kb on - strand, within Pf6N2E2_5960 at 6778.827 kb on - strand, within Pf6N2E2_5960 at 6779.036 kb on + strand, within Pf6N2E2_5960 at 6779.036 kb on + strand, within Pf6N2E2_5960 at 6779.037 kb on - strand, within Pf6N2E2_5960 at 6779.039 kb on + strand, within Pf6N2E2_5960 at 6779.039 kb on + strand, within Pf6N2E2_5960 at 6779.039 kb on + strand, within Pf6N2E2_5960 at 6779.040 kb on - strand, within Pf6N2E2_5960 at 6779.040 kb on - strand, within Pf6N2E2_5960 at 6779.144 kb on + strand, within Pf6N2E2_5960 at 6779.144 kb on + strand, within Pf6N2E2_5960 at 6779.145 kb on - strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.189 kb on + strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.190 kb on - strand, within Pf6N2E2_5960 at 6779.193 kb on + strand, within Pf6N2E2_5960 at 6779.193 kb on + strand, within Pf6N2E2_5960 at 6779.193 kb on + strand, within Pf6N2E2_5960 at 6779.194 kb on - strand, within Pf6N2E2_5960 at 6779.237 kb on + strand, within Pf6N2E2_5960 at 6779.237 kb on + strand, within Pf6N2E2_5960 at 6779.238 kb on - strand at 6779.378 kb on - strand at 6779.378 kb on - strand at 6779.378 kb on - strand at 6779.403 kb on + strand at 6779.404 kb on - strand at 6779.453 kb on + strand at 6779.453 kb on + strand at 6779.454 kb on - strand at 6779.454 kb on - strand at 6779.454 kb on - strand at 6779.454 kb on - strand at 6779.454 kb on - strand at 6779.463 kb on + strand at 6779.463 kb on + strand at 6779.463 kb on + strand at 6779.463 kb on + strand at 6779.463 kb on + strand at 6779.464 kb on - strand at 6779.659 kb on + strand at 6779.851 kb on + strand, within Pf6N2E2_5961 at 6779.851 kb on + strand, within Pf6N2E2_5961 at 6779.851 kb on + strand, within Pf6N2E2_5961 at 6779.851 kb on + strand, within Pf6N2E2_5961 at 6779.851 kb on + strand, within Pf6N2E2_5961 at 6779.852 kb on - strand, within Pf6N2E2_5961 at 6779.853 kb on + strand, within Pf6N2E2_5961 at 6779.853 kb on + strand, within Pf6N2E2_5961 at 6779.881 kb on + strand, within Pf6N2E2_5961 at 6779.881 kb on + strand, within Pf6N2E2_5961 at 6779.881 kb on + strand, within Pf6N2E2_5961 at 6779.881 kb on + strand, within Pf6N2E2_5961 at 6779.881 kb on + strand, within Pf6N2E2_5961 at 6779.882 kb on - strand, within Pf6N2E2_5961 at 6779.882 kb on - strand, within Pf6N2E2_5961 at 6779.882 kb on - strand, within Pf6N2E2_5961 at 6779.902 kb on + strand, within Pf6N2E2_5961 at 6779.902 kb on + strand, within Pf6N2E2_5961 at 6779.974 kb on + strand, within Pf6N2E2_5961 at 6779.974 kb on + strand, within Pf6N2E2_5961 at 6780.166 kb on + strand, within Pf6N2E2_5961 at 6780.175 kb on + strand, within Pf6N2E2_5961 at 6780.176 kb on - strand, within Pf6N2E2_5961 at 6780.217 kb on + strand, within Pf6N2E2_5961 at 6780.217 kb on + strand, within Pf6N2E2_5961 at 6780.218 kb on - strand, within Pf6N2E2_5961 at 6780.218 kb on - strand, within Pf6N2E2_5961
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,778,060 + -0.1 6,778,061 - -0.0 6,778,105 + -1.6 6,778,105 + +1.0 6,778,106 - -2.1 6,778,106 - +1.0 6,778,106 - +0.8 6,778,106 - -0.3 6,778,106 - -0.0 6,778,106 - -1.8 6,778,106 - +1.3 6,778,106 - -0.2 6,778,124 + +0.2 6,778,205 + Pf6N2E2_5959 0.14 +0.5 6,778,228 - Pf6N2E2_5959 0.20 -2.6 6,778,236 + Pf6N2E2_5959 0.23 +0.4 6,778,236 + Pf6N2E2_5959 0.23 -0.3 6,778,236 + Pf6N2E2_5959 0.23 -1.6 6,778,236 + Pf6N2E2_5959 0.23 -0.6 6,778,237 - Pf6N2E2_5959 0.23 +0.0 6,778,237 - Pf6N2E2_5959 0.23 +1.6 6,778,237 - Pf6N2E2_5959 0.23 -2.5 6,778,237 - Pf6N2E2_5959 0.23 +0.5 6,778,287 + Pf6N2E2_5959 0.37 -0.8 6,778,287 + Pf6N2E2_5959 0.37 -0.7 6,778,287 + Pf6N2E2_5959 0.37 +0.2 6,778,288 - Pf6N2E2_5959 0.37 +1.0 6,778,288 - Pf6N2E2_5959 0.37 -0.6 6,778,502 + -1.0 6,778,502 + -1.0 6,778,502 + +0.2 6,778,502 + -3.1 6,778,502 + -0.4 6,778,503 - -1.2 6,778,503 - -0.1 6,778,509 + -0.5 6,778,510 - -0.6 6,778,522 + -2.4 6,778,522 + -0.1 6,778,522 + -1.0 6,778,522 + -1.8 6,778,522 + -1.0 6,778,522 + -2.0 6,778,523 - -0.9 6,778,523 - -2.0 6,778,523 - -2.2 6,778,523 - +0.9 6,778,523 - +1.0 6,778,523 - -1.6 6,778,523 - -1.3 6,778,523 - -0.7 6,778,523 - +0.7 6,778,523 - -2.7 6,778,530 + -0.6 6,778,530 + +0.5 6,778,530 + +0.3 6,778,530 + -2.0 6,778,531 - +0.3 6,778,531 - +0.9 6,778,531 - +0.5 6,778,531 - -1.6 6,778,820 + Pf6N2E2_5960 0.37 -2.0 6,778,820 + Pf6N2E2_5960 0.37 -3.4 6,778,820 + Pf6N2E2_5960 0.37 -1.2 6,778,820 + Pf6N2E2_5960 0.37 -1.0 6,778,821 - Pf6N2E2_5960 0.37 -1.6 6,778,827 - Pf6N2E2_5960 0.38 -2.2 6,778,827 - Pf6N2E2_5960 0.38 +0.4 6,778,827 - Pf6N2E2_5960 0.38 -1.8 6,779,036 + Pf6N2E2_5960 0.65 +2.8 6,779,036 + Pf6N2E2_5960 0.65 -1.6 6,779,037 - Pf6N2E2_5960 0.65 -0.6 6,779,039 + Pf6N2E2_5960 0.65 -0.6 6,779,039 + Pf6N2E2_5960 0.65 -0.5 6,779,039 + Pf6N2E2_5960 0.65 -1.2 6,779,040 - Pf6N2E2_5960 0.65 +0.5 6,779,040 - Pf6N2E2_5960 0.65 -0.5 6,779,144 + Pf6N2E2_5960 0.78 -0.3 6,779,144 + Pf6N2E2_5960 0.78 +0.2 6,779,145 - Pf6N2E2_5960 0.78 -2.6 6,779,189 + Pf6N2E2_5960 0.84 -1.2 6,779,189 + Pf6N2E2_5960 0.84 -1.8 6,779,189 + Pf6N2E2_5960 0.84 -1.3 6,779,189 + Pf6N2E2_5960 0.84 -2.4 6,779,189 + Pf6N2E2_5960 0.84 -0.7 6,779,189 + Pf6N2E2_5960 0.84 -2.5 6,779,189 + Pf6N2E2_5960 0.84 -2.0 6,779,189 + Pf6N2E2_5960 0.84 -0.5 6,779,189 + Pf6N2E2_5960 0.84 -2.8 6,779,190 - Pf6N2E2_5960 0.84 -1.0 6,779,190 - Pf6N2E2_5960 0.84 +1.4 6,779,190 - Pf6N2E2_5960 0.84 -3.3 6,779,190 - Pf6N2E2_5960 0.84 -0.0 6,779,190 - Pf6N2E2_5960 0.84 -1.4 6,779,190 - Pf6N2E2_5960 0.84 -2.0 6,779,193 + Pf6N2E2_5960 0.84 -0.2 6,779,193 + Pf6N2E2_5960 0.84 -1.4 6,779,193 + Pf6N2E2_5960 0.84 -0.0 6,779,194 - Pf6N2E2_5960 0.84 -2.8 6,779,237 + Pf6N2E2_5960 0.90 -0.8 6,779,237 + Pf6N2E2_5960 0.90 -2.6 6,779,238 - +1.1 6,779,378 - +0.8 6,779,378 - +0.2 6,779,378 - +0.5 6,779,403 + -0.6 6,779,404 - -1.4 6,779,453 + -0.3 6,779,453 + -0.6 6,779,454 - -2.4 6,779,454 - +1.1 6,779,454 - -1.6 6,779,454 - +1.5 6,779,454 - +1.0 6,779,463 + -2.7 6,779,463 + -1.4 6,779,463 + -2.0 6,779,463 + -0.6 6,779,463 + -0.0 6,779,464 - -0.0 6,779,659 + +0.4 6,779,851 + Pf6N2E2_5961 0.23 -0.0 6,779,851 + Pf6N2E2_5961 0.23 -0.6 6,779,851 + Pf6N2E2_5961 0.23 -2.5 6,779,851 + Pf6N2E2_5961 0.23 +1.0 6,779,851 + Pf6N2E2_5961 0.23 -1.4 6,779,852 - Pf6N2E2_5961 0.24 -0.6 6,779,853 + Pf6N2E2_5961 0.24 -0.4 6,779,853 + Pf6N2E2_5961 0.24 -0.9 6,779,881 + Pf6N2E2_5961 0.28 -2.5 6,779,881 + Pf6N2E2_5961 0.28 -1.2 6,779,881 + Pf6N2E2_5961 0.28 -0.2 6,779,881 + Pf6N2E2_5961 0.28 -2.6 6,779,881 + Pf6N2E2_5961 0.28 -0.6 6,779,882 - Pf6N2E2_5961 0.28 +1.0 6,779,882 - Pf6N2E2_5961 0.28 +1.0 6,779,882 - Pf6N2E2_5961 0.28 +2.0 6,779,902 + Pf6N2E2_5961 0.31 -2.5 6,779,902 + Pf6N2E2_5961 0.31 -1.4 6,779,974 + Pf6N2E2_5961 0.43 +0.6 6,779,974 + Pf6N2E2_5961 0.43 +1.3 6,780,166 + Pf6N2E2_5961 0.73 -0.6 6,780,175 + Pf6N2E2_5961 0.74 -1.6 6,780,176 - Pf6N2E2_5961 0.74 +1.0 6,780,217 + Pf6N2E2_5961 0.81 +0.6 6,780,217 + Pf6N2E2_5961 0.81 -1.6 6,780,218 - Pf6N2E2_5961 0.81 -1.6 6,780,218 - Pf6N2E2_5961 0.81 +1.4
Or see this region's nucleotide sequence