Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5924 and Pf6N2E2_5925 are separated by 310 nucleotides Pf6N2E2_5925 and Pf6N2E2_5926 are separated by 45 nucleotides Pf6N2E2_5926 and Pf6N2E2_5927 are separated by 136 nucleotides
Pf6N2E2_5924 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 6,743,026 to 6,745,473
_5924
Pf6N2E2_5925 - Transglutaminase-like enzymes, putative cysteine proteases, at 6,745,784 to 6,746,536
_5925
Pf6N2E2_5926 - Uncharacterized glutathione S-transferase-like protein, at 6,746,582 to 6,747,205
_5926
Pf6N2E2_5927 - ABC-type multidrug transport system, ATPase component, at 6,747,342 to 6,748,274
_5927
Position (kb)
6745
6746
6747 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 6744.791 kb on + strand, within Pf6N2E2_5924 at 6744.792 kb on - strand, within Pf6N2E2_5924 at 6744.923 kb on + strand, within Pf6N2E2_5924 at 6744.948 kb on - strand, within Pf6N2E2_5924 at 6744.948 kb on - strand, within Pf6N2E2_5924 at 6745.376 kb on + strand at 6745.376 kb on + strand at 6745.377 kb on - strand at 6745.377 kb on - strand at 6745.377 kb on - strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.473 kb on + strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.474 kb on - strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.477 kb on + strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.478 kb on - strand at 6745.518 kb on - strand at 6745.779 kb on + strand at 6745.779 kb on + strand at 6745.841 kb on - strand at 6745.841 kb on - strand at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.874 kb on + strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.875 kb on - strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.876 kb on + strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.877 kb on - strand, within Pf6N2E2_5925 at 6745.887 kb on - strand, within Pf6N2E2_5925 at 6745.887 kb on - strand, within Pf6N2E2_5925 at 6745.940 kb on - strand, within Pf6N2E2_5925 at 6745.994 kb on + strand, within Pf6N2E2_5925 at 6745.994 kb on + strand, within Pf6N2E2_5925 at 6745.995 kb on - strand, within Pf6N2E2_5925 at 6745.997 kb on + strand, within Pf6N2E2_5925 at 6745.998 kb on - strand, within Pf6N2E2_5925 at 6745.998 kb on - strand, within Pf6N2E2_5925 at 6745.998 kb on - strand, within Pf6N2E2_5925 at 6746.021 kb on + strand, within Pf6N2E2_5925 at 6746.021 kb on + strand, within Pf6N2E2_5925 at 6746.022 kb on - strand, within Pf6N2E2_5925 at 6746.030 kb on + strand, within Pf6N2E2_5925 at 6746.030 kb on + strand, within Pf6N2E2_5925 at 6746.031 kb on - strand, within Pf6N2E2_5925 at 6746.031 kb on - strand, within Pf6N2E2_5925 at 6746.069 kb on + strand, within Pf6N2E2_5925 at 6746.093 kb on + strand, within Pf6N2E2_5925 at 6746.093 kb on + strand, within Pf6N2E2_5925 at 6746.093 kb on + strand, within Pf6N2E2_5925 at 6746.093 kb on + strand, within Pf6N2E2_5925 at 6746.094 kb on - strand, within Pf6N2E2_5925 at 6746.094 kb on - strand, within Pf6N2E2_5925 at 6746.242 kb on + strand, within Pf6N2E2_5925 at 6746.243 kb on - strand, within Pf6N2E2_5925 at 6746.243 kb on - strand, within Pf6N2E2_5925 at 6746.253 kb on - strand, within Pf6N2E2_5925 at 6746.253 kb on - strand, within Pf6N2E2_5925 at 6746.396 kb on + strand, within Pf6N2E2_5925 at 6746.396 kb on + strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.397 kb on - strand, within Pf6N2E2_5925 at 6746.466 kb on - strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.544 kb on + strand at 6746.545 kb on - strand at 6746.561 kb on + strand at 6746.561 kb on + strand at 6746.562 kb on - strand at 6746.660 kb on + strand, within Pf6N2E2_5926 at 6746.844 kb on + strand, within Pf6N2E2_5926 at 6747.035 kb on + strand, within Pf6N2E2_5926 at 6747.036 kb on - strand, within Pf6N2E2_5926 at 6747.036 kb on - strand, within Pf6N2E2_5926 at 6747.089 kb on + strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.090 kb on - strand, within Pf6N2E2_5926 at 6747.127 kb on - strand, within Pf6N2E2_5926 at 6747.127 kb on - strand, within Pf6N2E2_5926 at 6747.147 kb on - strand at 6747.147 kb on - strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.203 kb on + strand at 6747.204 kb on - strand at 6747.204 kb on - strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.206 kb on + strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.207 kb on - strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.210 kb on + strand at 6747.211 kb on - strand at 6747.211 kb on - strand at 6747.211 kb on - strand at 6747.214 kb on - strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.231 kb on + strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.232 kb on - strand at 6747.299 kb on + strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.300 kb on - strand at 6747.303 kb on + strand at 6747.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,744,791 + Pf6N2E2_5924 0.72 +0.2 6,744,792 - Pf6N2E2_5924 0.72 +1.2 6,744,923 + Pf6N2E2_5924 0.77 +0.5 6,744,948 - Pf6N2E2_5924 0.79 -0.8 6,744,948 - Pf6N2E2_5924 0.79 -2.5 6,745,376 + +0.7 6,745,376 + -1.8 6,745,377 - +1.7 6,745,377 - -0.3 6,745,377 - +2.4 6,745,473 + +0.2 6,745,473 + -1.0 6,745,473 + -0.6 6,745,473 + +1.3 6,745,473 + +1.0 6,745,473 + +1.6 6,745,473 + +0.7 6,745,473 + +0.3 6,745,473 + -3.4 6,745,473 + -0.0 6,745,473 + -0.2 6,745,473 + -0.0 6,745,473 + -0.4 6,745,473 + +1.5 6,745,473 + -0.0 6,745,474 - +0.3 6,745,474 - -0.1 6,745,474 - +0.3 6,745,474 - -1.2 6,745,474 - +1.3 6,745,474 - +0.7 6,745,474 - +0.3 6,745,474 - +0.2 6,745,474 - +0.3 6,745,474 - +0.3 6,745,474 - -0.1 6,745,474 - +1.0 6,745,474 - +0.8 6,745,474 - -0.6 6,745,477 + -0.6 6,745,477 + -2.0 6,745,477 + +1.0 6,745,477 + -1.8 6,745,477 + -1.2 6,745,477 + +0.2 6,745,477 + +0.4 6,745,477 + -1.0 6,745,477 + -0.0 6,745,477 + -1.3 6,745,477 + -0.1 6,745,477 + -1.3 6,745,477 + -2.9 6,745,477 + +1.0 6,745,477 + -1.3 6,745,477 + -1.8 6,745,477 + +0.6 6,745,478 - -0.2 6,745,478 - +1.2 6,745,478 - -0.8 6,745,478 - -2.2 6,745,478 - +1.0 6,745,478 - +1.0 6,745,478 - -0.0 6,745,478 - -0.0 6,745,518 - +1.0 6,745,779 + +0.7 6,745,779 + -0.3 6,745,841 - +1.9 6,745,841 - +0.5 6,745,874 + Pf6N2E2_5925 0.12 -0.6 6,745,874 + Pf6N2E2_5925 0.12 -0.4 6,745,874 + Pf6N2E2_5925 0.12 -2.5 6,745,874 + Pf6N2E2_5925 0.12 +1.0 6,745,874 + Pf6N2E2_5925 0.12 -3.1 6,745,874 + Pf6N2E2_5925 0.12 +0.1 6,745,874 + Pf6N2E2_5925 0.12 -2.2 6,745,874 + Pf6N2E2_5925 0.12 -1.6 6,745,875 - Pf6N2E2_5925 0.12 +1.8 6,745,875 - Pf6N2E2_5925 0.12 -0.3 6,745,875 - Pf6N2E2_5925 0.12 -0.3 6,745,875 - Pf6N2E2_5925 0.12 -1.1 6,745,875 - Pf6N2E2_5925 0.12 +2.6 6,745,875 - Pf6N2E2_5925 0.12 -0.6 6,745,875 - Pf6N2E2_5925 0.12 +0.8 6,745,876 + Pf6N2E2_5925 0.12 -0.5 6,745,876 + Pf6N2E2_5925 0.12 -0.6 6,745,876 + Pf6N2E2_5925 0.12 +1.0 6,745,876 + Pf6N2E2_5925 0.12 -0.9 6,745,876 + Pf6N2E2_5925 0.12 +0.3 6,745,876 + Pf6N2E2_5925 0.12 +1.1 6,745,876 + Pf6N2E2_5925 0.12 -1.3 6,745,876 + Pf6N2E2_5925 0.12 +0.7 6,745,876 + Pf6N2E2_5925 0.12 -0.0 6,745,876 + Pf6N2E2_5925 0.12 +0.1 6,745,876 + Pf6N2E2_5925 0.12 -0.3 6,745,876 + Pf6N2E2_5925 0.12 +0.8 6,745,877 - Pf6N2E2_5925 0.12 +0.9 6,745,877 - Pf6N2E2_5925 0.12 +2.1 6,745,877 - Pf6N2E2_5925 0.12 +0.6 6,745,877 - Pf6N2E2_5925 0.12 +0.4 6,745,877 - Pf6N2E2_5925 0.12 -1.2 6,745,877 - Pf6N2E2_5925 0.12 -1.0 6,745,877 - Pf6N2E2_5925 0.12 +1.5 6,745,877 - Pf6N2E2_5925 0.12 -0.3 6,745,877 - Pf6N2E2_5925 0.12 +0.9 6,745,877 - Pf6N2E2_5925 0.12 +1.3 6,745,877 - Pf6N2E2_5925 0.12 +0.5 6,745,887 - Pf6N2E2_5925 0.14 +1.3 6,745,887 - Pf6N2E2_5925 0.14 +1.4 6,745,940 - Pf6N2E2_5925 0.21 +1.0 6,745,994 + Pf6N2E2_5925 0.28 -0.4 6,745,994 + Pf6N2E2_5925 0.28 +0.6 6,745,995 - Pf6N2E2_5925 0.28 +0.1 6,745,997 + Pf6N2E2_5925 0.28 +0.5 6,745,998 - Pf6N2E2_5925 0.28 +1.9 6,745,998 - Pf6N2E2_5925 0.28 +3.6 6,745,998 - Pf6N2E2_5925 0.28 +2.6 6,746,021 + Pf6N2E2_5925 0.31 +1.0 6,746,021 + Pf6N2E2_5925 0.31 +0.7 6,746,022 - Pf6N2E2_5925 0.32 +0.9 6,746,030 + Pf6N2E2_5925 0.33 -0.8 6,746,030 + Pf6N2E2_5925 0.33 -0.2 6,746,031 - Pf6N2E2_5925 0.33 -1.0 6,746,031 - Pf6N2E2_5925 0.33 -0.2 6,746,069 + Pf6N2E2_5925 0.38 +0.4 6,746,093 + Pf6N2E2_5925 0.41 +1.1 6,746,093 + Pf6N2E2_5925 0.41 -0.0 6,746,093 + Pf6N2E2_5925 0.41 +1.0 6,746,093 + Pf6N2E2_5925 0.41 +0.9 6,746,094 - Pf6N2E2_5925 0.41 +2.0 6,746,094 - Pf6N2E2_5925 0.41 +1.0 6,746,242 + Pf6N2E2_5925 0.61 +1.2 6,746,243 - Pf6N2E2_5925 0.61 +1.0 6,746,243 - Pf6N2E2_5925 0.61 +0.1 6,746,253 - Pf6N2E2_5925 0.62 +0.9 6,746,253 - Pf6N2E2_5925 0.62 +0.8 6,746,396 + Pf6N2E2_5925 0.81 -1.3 6,746,396 + Pf6N2E2_5925 0.81 -3.0 6,746,397 - Pf6N2E2_5925 0.81 -0.2 6,746,397 - Pf6N2E2_5925 0.81 +0.2 6,746,397 - Pf6N2E2_5925 0.81 +0.7 6,746,466 - +0.5 6,746,544 + +0.4 6,746,544 + -1.0 6,746,544 + -1.0 6,746,544 + +1.4 6,746,545 - -0.1 6,746,561 + -1.1 6,746,561 + -2.5 6,746,562 - +0.1 6,746,660 + Pf6N2E2_5926 0.12 -1.5 6,746,844 + Pf6N2E2_5926 0.42 -0.8 6,747,035 + Pf6N2E2_5926 0.73 +0.4 6,747,036 - Pf6N2E2_5926 0.73 +0.9 6,747,036 - Pf6N2E2_5926 0.73 -1.5 6,747,089 + Pf6N2E2_5926 0.81 +0.1 6,747,090 - Pf6N2E2_5926 0.81 -0.6 6,747,090 - Pf6N2E2_5926 0.81 -0.0 6,747,090 - Pf6N2E2_5926 0.81 -0.1 6,747,090 - Pf6N2E2_5926 0.81 -0.6 6,747,127 - Pf6N2E2_5926 0.87 -1.6 6,747,127 - Pf6N2E2_5926 0.87 +3.8 6,747,147 - +2.0 6,747,147 - +0.4 6,747,203 + -1.0 6,747,203 + +1.4 6,747,203 + +0.6 6,747,203 + +0.8 6,747,203 + -0.0 6,747,204 - -0.5 6,747,204 - +0.3 6,747,206 + +0.4 6,747,206 + +1.8 6,747,206 + +1.0 6,747,206 + -0.0 6,747,206 + -2.8 6,747,207 - -2.3 6,747,207 - -1.3 6,747,207 - -2.3 6,747,207 - +0.9 6,747,207 - +0.3 6,747,210 + +0.1 6,747,210 + +0.8 6,747,210 + -0.0 6,747,210 + -0.0 6,747,210 + -1.8 6,747,210 + +0.1 6,747,211 - +0.8 6,747,211 - -0.9 6,747,211 - -0.2 6,747,214 - -0.2 6,747,231 + +0.4 6,747,231 + -0.0 6,747,231 + +0.8 6,747,231 + +1.5 6,747,232 - -1.7 6,747,232 - -0.1 6,747,232 - -3.2 6,747,232 - -2.0 6,747,299 + -1.0 6,747,300 - -0.4 6,747,300 - -0.6 6,747,300 - -0.3 6,747,300 - -0.3 6,747,303 + +2.0 6,747,334 - -2.0
Or see this region's nucleotide sequence