Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5724 and Pf6N2E2_5725 are separated by 26 nucleotides Pf6N2E2_5725 and Pf6N2E2_5726 are separated by 130 nucleotides Pf6N2E2_5726 and Pf6N2E2_5727 are separated by 276 nucleotides Pf6N2E2_5727 and Pf6N2E2_5728 are separated by 8 nucleotides
Pf6N2E2_5724 - Nucleoside triphosphate pyrophosphohydrolase MazG (EC 3.6.1.8), at 6,542,282 to 6,543,115
_5724
Pf6N2E2_5725 - nucleoprotein/polynucleotide-associated enzyme, at 6,543,142 to 6,543,681
_5725
Pf6N2E2_5726 - FIG00953102: hypothetical protein, at 6,543,812 to 6,544,006
_5726
Pf6N2E2_5727 - FIG00955360: hypothetical protein, at 6,544,283 to 6,544,996
_5727
Pf6N2E2_5728 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 6,545,005 to 6,545,655
_5728
Position (kb)
6543
6544
6545 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6542.850 kb on - strand, within Pf6N2E2_5724 at 6542.999 kb on - strand, within Pf6N2E2_5724 at 6543.126 kb on + strand at 6543.126 kb on + strand at 6543.127 kb on - strand at 6543.430 kb on + strand, within Pf6N2E2_5725 at 6543.430 kb on + strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.431 kb on - strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.433 kb on + strand, within Pf6N2E2_5725 at 6543.434 kb on - strand, within Pf6N2E2_5725 at 6543.652 kb on + strand at 6543.762 kb on + strand at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.870 kb on + strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.871 kb on - strand, within Pf6N2E2_5726 at 6543.874 kb on + strand, within Pf6N2E2_5726 at 6543.874 kb on + strand, within Pf6N2E2_5726 at 6543.875 kb on - strand, within Pf6N2E2_5726 at 6543.875 kb on - strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.970 kb on + strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.971 kb on - strand, within Pf6N2E2_5726 at 6543.973 kb on - strand, within Pf6N2E2_5726 at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6543.993 kb on - strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.028 kb on + strand at 6544.029 kb on - strand at 6544.029 kb on - strand at 6544.062 kb on - strand at 6544.080 kb on - strand at 6544.083 kb on + strand at 6544.084 kb on - strand at 6544.084 kb on - strand at 6544.084 kb on - strand at 6544.125 kb on + strand at 6544.208 kb on + strand at 6544.208 kb on + strand at 6544.208 kb on + strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.209 kb on - strand at 6544.241 kb on + strand at 6544.241 kb on + strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.242 kb on - strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.338 kb on + strand at 6544.339 kb on - strand at 6544.383 kb on + strand, within Pf6N2E2_5727 at 6544.384 kb on - strand, within Pf6N2E2_5727 at 6544.518 kb on + strand, within Pf6N2E2_5727 at 6544.518 kb on + strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.519 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.523 kb on - strand, within Pf6N2E2_5727 at 6544.551 kb on + strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.552 kb on - strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.602 kb on + strand, within Pf6N2E2_5727 at 6544.603 kb on - strand, within Pf6N2E2_5727 at 6544.603 kb on - strand, within Pf6N2E2_5727 at 6544.743 kb on + strand, within Pf6N2E2_5727 at 6544.744 kb on - strand, within Pf6N2E2_5727 at 6544.840 kb on + strand, within Pf6N2E2_5727 at 6544.911 kb on + strand, within Pf6N2E2_5727 at 6544.912 kb on - strand, within Pf6N2E2_5727 at 6544.931 kb on + strand at 6544.932 kb on - strand at 6544.932 kb on - strand at 6544.932 kb on - strand at 6544.997 kb on - strand at 6545.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,542,850 - Pf6N2E2_5724 0.68 -2.9 6,542,999 - Pf6N2E2_5724 0.86 +0.1 6,543,126 + -0.9 6,543,126 + -0.3 6,543,127 - +0.1 6,543,430 + Pf6N2E2_5725 0.53 -1.8 6,543,430 + Pf6N2E2_5725 0.53 -0.9 6,543,431 - Pf6N2E2_5725 0.54 -0.0 6,543,431 - Pf6N2E2_5725 0.54 -1.4 6,543,431 - Pf6N2E2_5725 0.54 -0.1 6,543,433 + Pf6N2E2_5725 0.54 -1.8 6,543,433 + Pf6N2E2_5725 0.54 +0.8 6,543,433 + Pf6N2E2_5725 0.54 -0.9 6,543,433 + Pf6N2E2_5725 0.54 -1.7 6,543,433 + Pf6N2E2_5725 0.54 +1.2 6,543,433 + Pf6N2E2_5725 0.54 +0.9 6,543,433 + Pf6N2E2_5725 0.54 -0.9 6,543,434 - Pf6N2E2_5725 0.54 -1.3 6,543,652 + +0.1 6,543,762 + +0.2 6,543,870 + Pf6N2E2_5726 0.30 -2.3 6,543,870 + Pf6N2E2_5726 0.30 -0.1 6,543,870 + Pf6N2E2_5726 0.30 +0.6 6,543,870 + Pf6N2E2_5726 0.30 -0.2 6,543,870 + Pf6N2E2_5726 0.30 +0.2 6,543,870 + Pf6N2E2_5726 0.30 -0.1 6,543,870 + Pf6N2E2_5726 0.30 -2.1 6,543,870 + Pf6N2E2_5726 0.30 +0.6 6,543,871 - Pf6N2E2_5726 0.30 -1.9 6,543,871 - Pf6N2E2_5726 0.30 -0.1 6,543,871 - Pf6N2E2_5726 0.30 +0.0 6,543,871 - Pf6N2E2_5726 0.30 -1.0 6,543,871 - Pf6N2E2_5726 0.30 +0.0 6,543,871 - Pf6N2E2_5726 0.30 +0.1 6,543,871 - Pf6N2E2_5726 0.30 -1.0 6,543,871 - Pf6N2E2_5726 0.30 -2.4 6,543,871 - Pf6N2E2_5726 0.30 +2.0 6,543,871 - Pf6N2E2_5726 0.30 -0.1 6,543,871 - Pf6N2E2_5726 0.30 -0.3 6,543,874 + Pf6N2E2_5726 0.32 +1.2 6,543,874 + Pf6N2E2_5726 0.32 -0.5 6,543,875 - Pf6N2E2_5726 0.32 -0.4 6,543,875 - Pf6N2E2_5726 0.32 +1.4 6,543,970 + Pf6N2E2_5726 0.81 +1.0 6,543,970 + Pf6N2E2_5726 0.81 -0.7 6,543,970 + Pf6N2E2_5726 0.81 -0.4 6,543,970 + Pf6N2E2_5726 0.81 -0.5 6,543,970 + Pf6N2E2_5726 0.81 +0.5 6,543,970 + Pf6N2E2_5726 0.81 +0.1 6,543,970 + Pf6N2E2_5726 0.81 -0.1 6,543,970 + Pf6N2E2_5726 0.81 -2.8 6,543,970 + Pf6N2E2_5726 0.81 -0.5 6,543,970 + Pf6N2E2_5726 0.81 -2.0 6,543,970 + Pf6N2E2_5726 0.81 -3.0 6,543,970 + Pf6N2E2_5726 0.81 -1.1 6,543,971 - Pf6N2E2_5726 0.82 -1.3 6,543,971 - Pf6N2E2_5726 0.82 +1.3 6,543,971 - Pf6N2E2_5726 0.82 -3.7 6,543,971 - Pf6N2E2_5726 0.82 -2.1 6,543,971 - Pf6N2E2_5726 0.82 +0.3 6,543,973 - Pf6N2E2_5726 0.83 +0.7 6,543,993 - +0.0 6,543,993 - +0.3 6,543,993 - +0.1 6,543,993 - -2.3 6,543,993 - -0.3 6,544,028 + -0.8 6,544,028 + -0.5 6,544,028 + +0.0 6,544,028 + +0.4 6,544,029 - +0.6 6,544,029 - -1.5 6,544,062 - +0.0 6,544,080 - -1.0 6,544,083 + -0.8 6,544,084 - -1.2 6,544,084 - -1.0 6,544,084 - -0.7 6,544,125 + -0.8 6,544,208 + -1.6 6,544,208 + -0.9 6,544,208 + +0.5 6,544,209 - +0.0 6,544,209 - -0.9 6,544,209 - +0.3 6,544,209 - +1.2 6,544,241 + +0.0 6,544,241 + -0.8 6,544,242 - +1.9 6,544,242 - -0.2 6,544,242 - -0.1 6,544,242 - +1.0 6,544,338 + +2.1 6,544,338 + -0.5 6,544,338 + -1.9 6,544,338 + -1.1 6,544,338 + -2.5 6,544,339 - +0.5 6,544,383 + Pf6N2E2_5727 0.14 +1.4 6,544,384 - Pf6N2E2_5727 0.14 +0.8 6,544,518 + Pf6N2E2_5727 0.33 +0.6 6,544,518 + Pf6N2E2_5727 0.33 +1.3 6,544,519 - Pf6N2E2_5727 0.33 +0.9 6,544,519 - Pf6N2E2_5727 0.33 -2.2 6,544,519 - Pf6N2E2_5727 0.33 +1.0 6,544,523 - Pf6N2E2_5727 0.34 +0.5 6,544,523 - Pf6N2E2_5727 0.34 +0.8 6,544,523 - Pf6N2E2_5727 0.34 +0.9 6,544,551 + Pf6N2E2_5727 0.38 -0.5 6,544,552 - Pf6N2E2_5727 0.38 +0.2 6,544,552 - Pf6N2E2_5727 0.38 +0.6 6,544,552 - Pf6N2E2_5727 0.38 +0.1 6,544,602 + Pf6N2E2_5727 0.45 +1.5 6,544,602 + Pf6N2E2_5727 0.45 +0.7 6,544,602 + Pf6N2E2_5727 0.45 -0.8 6,544,603 - Pf6N2E2_5727 0.45 +1.9 6,544,603 - Pf6N2E2_5727 0.45 -0.2 6,544,743 + Pf6N2E2_5727 0.64 -0.5 6,544,744 - Pf6N2E2_5727 0.65 -0.3 6,544,840 + Pf6N2E2_5727 0.78 +0.1 6,544,911 + Pf6N2E2_5727 0.88 +0.5 6,544,912 - Pf6N2E2_5727 0.88 +1.0 6,544,931 + -2.4 6,544,932 - +0.4 6,544,932 - +0.8 6,544,932 - +2.4 6,544,997 - +0.6 6,545,006 + +1.1
Or see this region's nucleotide sequence