Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5680 and Pf6N2E2_5681 are separated by 116 nucleotides Pf6N2E2_5681 and Pf6N2E2_5682 are separated by 79 nucleotides
Pf6N2E2_5680 - Mg/Co/Ni transporter MgtE / CBS domain, at 6,493,015 to 6,494,457
_5680
Pf6N2E2_5681 - RhtB family transporter, at 6,494,574 to 6,495,188
_5681
Pf6N2E2_5682 - Transcriptional regulator, AraC family, at 6,495,268 to 6,496,080
_5682
Position (kb)
6494
6495
6496 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 6494.489 kb on + strand at 6494.489 kb on + strand at 6494.489 kb on + strand at 6494.490 kb on - strand at 6494.490 kb on - strand at 6494.490 kb on - strand at 6494.492 kb on + strand at 6494.492 kb on + strand at 6494.492 kb on + strand at 6494.492 kb on + strand at 6494.492 kb on + strand at 6494.493 kb on - strand at 6494.493 kb on - strand at 6494.493 kb on - strand at 6494.493 kb on - strand at 6494.503 kb on + strand at 6494.503 kb on + strand at 6494.503 kb on + strand at 6494.504 kb on - strand at 6494.589 kb on + strand at 6494.611 kb on - strand at 6494.705 kb on - strand, within Pf6N2E2_5681 at 6494.705 kb on - strand, within Pf6N2E2_5681 at 6494.705 kb on - strand, within Pf6N2E2_5681 at 6494.705 kb on - strand, within Pf6N2E2_5681 at 6494.705 kb on - strand, within Pf6N2E2_5681 at 6494.802 kb on + strand, within Pf6N2E2_5681 at 6494.906 kb on + strand, within Pf6N2E2_5681 at 6494.906 kb on + strand, within Pf6N2E2_5681 at 6494.907 kb on - strand, within Pf6N2E2_5681 at 6494.907 kb on - strand, within Pf6N2E2_5681 at 6494.907 kb on - strand, within Pf6N2E2_5681 at 6494.907 kb on - strand, within Pf6N2E2_5681 at 6494.956 kb on + strand, within Pf6N2E2_5681 at 6494.956 kb on + strand, within Pf6N2E2_5681 at 6494.957 kb on - strand, within Pf6N2E2_5681 at 6494.957 kb on - strand, within Pf6N2E2_5681 at 6494.971 kb on + strand, within Pf6N2E2_5681 at 6494.971 kb on + strand, within Pf6N2E2_5681 at 6494.971 kb on + strand, within Pf6N2E2_5681 at 6494.971 kb on + strand, within Pf6N2E2_5681 at 6494.972 kb on - strand, within Pf6N2E2_5681 at 6494.972 kb on - strand, within Pf6N2E2_5681 at 6495.062 kb on + strand, within Pf6N2E2_5681 at 6495.062 kb on + strand, within Pf6N2E2_5681 at 6495.063 kb on - strand, within Pf6N2E2_5681 at 6495.063 kb on - strand, within Pf6N2E2_5681 at 6495.063 kb on - strand, within Pf6N2E2_5681 at 6495.063 kb on - strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.122 kb on + strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.123 kb on - strand, within Pf6N2E2_5681 at 6495.124 kb on + strand, within Pf6N2E2_5681 at 6495.124 kb on + strand, within Pf6N2E2_5681 at 6495.124 kb on + strand, within Pf6N2E2_5681 at 6495.124 kb on + strand, within Pf6N2E2_5681 at 6495.125 kb on - strand, within Pf6N2E2_5681 at 6495.125 kb on - strand, within Pf6N2E2_5681 at 6495.171 kb on - strand at 6495.171 kb on - strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.290 kb on + strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.291 kb on - strand at 6495.336 kb on + strand at 6495.337 kb on - strand at 6495.337 kb on - strand at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.438 kb on - strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.698 kb on + strand, within Pf6N2E2_5682 at 6495.699 kb on - strand, within Pf6N2E2_5682 at 6495.787 kb on - strand, within Pf6N2E2_5682 at 6495.824 kb on + strand, within Pf6N2E2_5682 at 6495.824 kb on + strand, within Pf6N2E2_5682 at 6495.825 kb on - strand, within Pf6N2E2_5682 at 6495.896 kb on - strand, within Pf6N2E2_5682 at 6495.896 kb on - strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.897 kb on + strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.898 kb on - strand, within Pf6N2E2_5682 at 6495.919 kb on + strand, within Pf6N2E2_5682 at 6495.919 kb on + strand, within Pf6N2E2_5682 at 6495.920 kb on - strand, within Pf6N2E2_5682 at 6495.920 kb on - strand, within Pf6N2E2_5682 at 6495.990 kb on + strand, within Pf6N2E2_5682 at 6495.990 kb on + strand, within Pf6N2E2_5682 at 6495.991 kb on - strand, within Pf6N2E2_5682 at 6495.991 kb on - strand, within Pf6N2E2_5682 at 6496.118 kb on + strand at 6496.119 kb on - strand at 6496.163 kb on + strand at 6496.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,494,489 + +2.7 6,494,489 + +1.5 6,494,489 + +2.5 6,494,490 - +4.9 6,494,490 - +6.2 6,494,490 - +2.8 6,494,492 + +4.1 6,494,492 + +1.5 6,494,492 + -0.9 6,494,492 + +2.7 6,494,492 + +4.0 6,494,493 - -0.9 6,494,493 - -1.4 6,494,493 - -0.5 6,494,493 - +4.3 6,494,503 + +3.7 6,494,503 + +2.7 6,494,503 + +3.4 6,494,504 - +3.4 6,494,589 + +0.3 6,494,611 - +4.4 6,494,705 - Pf6N2E2_5681 0.21 +2.1 6,494,705 - Pf6N2E2_5681 0.21 +1.3 6,494,705 - Pf6N2E2_5681 0.21 -0.9 6,494,705 - Pf6N2E2_5681 0.21 +2.1 6,494,705 - Pf6N2E2_5681 0.21 +1.0 6,494,802 + Pf6N2E2_5681 0.37 +0.7 6,494,906 + Pf6N2E2_5681 0.54 -0.5 6,494,906 + Pf6N2E2_5681 0.54 +1.4 6,494,907 - Pf6N2E2_5681 0.54 +2.9 6,494,907 - Pf6N2E2_5681 0.54 -0.7 6,494,907 - Pf6N2E2_5681 0.54 +2.0 6,494,907 - Pf6N2E2_5681 0.54 -1.7 6,494,956 + Pf6N2E2_5681 0.62 +0.1 6,494,956 + Pf6N2E2_5681 0.62 +1.2 6,494,957 - Pf6N2E2_5681 0.62 +1.4 6,494,957 - Pf6N2E2_5681 0.62 +2.7 6,494,971 + Pf6N2E2_5681 0.65 -0.1 6,494,971 + Pf6N2E2_5681 0.65 +1.0 6,494,971 + Pf6N2E2_5681 0.65 +1.4 6,494,971 + Pf6N2E2_5681 0.65 +2.3 6,494,972 - Pf6N2E2_5681 0.65 +2.1 6,494,972 - Pf6N2E2_5681 0.65 +2.0 6,495,062 + Pf6N2E2_5681 0.79 +0.9 6,495,062 + Pf6N2E2_5681 0.79 +1.7 6,495,063 - Pf6N2E2_5681 0.80 -0.1 6,495,063 - Pf6N2E2_5681 0.80 -0.9 6,495,063 - Pf6N2E2_5681 0.80 -1.2 6,495,063 - Pf6N2E2_5681 0.80 +0.2 6,495,122 + Pf6N2E2_5681 0.89 +2.1 6,495,122 + Pf6N2E2_5681 0.89 +0.9 6,495,122 + Pf6N2E2_5681 0.89 -0.6 6,495,122 + Pf6N2E2_5681 0.89 +1.1 6,495,122 + Pf6N2E2_5681 0.89 -1.9 6,495,122 + Pf6N2E2_5681 0.89 -1.6 6,495,122 + Pf6N2E2_5681 0.89 +0.2 6,495,122 + Pf6N2E2_5681 0.89 +0.1 6,495,122 + Pf6N2E2_5681 0.89 +1.1 6,495,123 - Pf6N2E2_5681 0.89 +0.6 6,495,123 - Pf6N2E2_5681 0.89 +1.7 6,495,123 - Pf6N2E2_5681 0.89 -0.5 6,495,123 - Pf6N2E2_5681 0.89 +1.3 6,495,123 - Pf6N2E2_5681 0.89 +1.9 6,495,123 - Pf6N2E2_5681 0.89 +1.3 6,495,124 + Pf6N2E2_5681 0.89 -0.0 6,495,124 + Pf6N2E2_5681 0.89 +1.1 6,495,124 + Pf6N2E2_5681 0.89 +2.7 6,495,124 + Pf6N2E2_5681 0.89 +0.5 6,495,125 - Pf6N2E2_5681 0.90 +1.7 6,495,125 - Pf6N2E2_5681 0.90 +1.1 6,495,171 - +0.7 6,495,171 - +4.0 6,495,290 + +0.3 6,495,290 + -1.2 6,495,290 + -0.0 6,495,290 + +1.1 6,495,290 + +0.4 6,495,290 + +0.8 6,495,290 + +1.4 6,495,290 + +0.6 6,495,291 - -0.8 6,495,291 - +0.8 6,495,291 - -0.5 6,495,291 - +0.5 6,495,291 - +1.0 6,495,336 + +1.1 6,495,337 - +1.4 6,495,337 - +1.3 6,495,438 - Pf6N2E2_5682 0.21 +1.2 6,495,438 - Pf6N2E2_5682 0.21 +1.1 6,495,438 - Pf6N2E2_5682 0.21 +0.1 6,495,698 + Pf6N2E2_5682 0.53 +1.2 6,495,698 + Pf6N2E2_5682 0.53 -2.4 6,495,698 + Pf6N2E2_5682 0.53 -2.1 6,495,699 - Pf6N2E2_5682 0.53 -0.2 6,495,787 - Pf6N2E2_5682 0.64 -0.1 6,495,824 + Pf6N2E2_5682 0.68 -2.4 6,495,824 + Pf6N2E2_5682 0.68 -0.5 6,495,825 - Pf6N2E2_5682 0.69 +0.8 6,495,896 - Pf6N2E2_5682 0.77 +1.3 6,495,896 - Pf6N2E2_5682 0.77 +1.8 6,495,897 + Pf6N2E2_5682 0.77 +1.1 6,495,897 + Pf6N2E2_5682 0.77 +1.0 6,495,897 + Pf6N2E2_5682 0.77 +0.5 6,495,897 + Pf6N2E2_5682 0.77 +1.9 6,495,897 + Pf6N2E2_5682 0.77 +0.1 6,495,897 + Pf6N2E2_5682 0.77 +1.5 6,495,897 + Pf6N2E2_5682 0.77 +0.8 6,495,897 + Pf6N2E2_5682 0.77 +1.1 6,495,897 + Pf6N2E2_5682 0.77 +0.7 6,495,898 - Pf6N2E2_5682 0.77 -0.4 6,495,898 - Pf6N2E2_5682 0.77 +1.6 6,495,898 - Pf6N2E2_5682 0.77 +1.5 6,495,898 - Pf6N2E2_5682 0.77 +2.1 6,495,898 - Pf6N2E2_5682 0.77 +2.3 6,495,898 - Pf6N2E2_5682 0.77 -0.9 6,495,898 - Pf6N2E2_5682 0.77 +1.5 6,495,898 - Pf6N2E2_5682 0.77 +0.5 6,495,898 - Pf6N2E2_5682 0.77 -0.1 6,495,898 - Pf6N2E2_5682 0.77 +0.1 6,495,919 + Pf6N2E2_5682 0.80 +1.6 6,495,919 + Pf6N2E2_5682 0.80 -0.5 6,495,920 - Pf6N2E2_5682 0.80 +0.8 6,495,920 - Pf6N2E2_5682 0.80 -0.2 6,495,990 + Pf6N2E2_5682 0.89 +0.2 6,495,990 + Pf6N2E2_5682 0.89 +1.1 6,495,991 - Pf6N2E2_5682 0.89 -1.5 6,495,991 - Pf6N2E2_5682 0.89 +0.1 6,496,118 + +0.7 6,496,119 - +4.1 6,496,163 + +4.1 6,496,164 - +2.6
Or see this region's nucleotide sequence