Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5476 and Pf6N2E2_5477 are separated by 215 nucleotides Pf6N2E2_5477 and Pf6N2E2_5478 are separated by 160 nucleotides
Pf6N2E2_5476 - Aldehyde dehydrogenase (EC 1.2.1.3), at 6,293,403 to 6,294,851
_5476
Pf6N2E2_5477 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 6,295,067 to 6,296,170
_5477
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Position (kb)
6295
6296
6297 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6294.406 kb on - strand, within Pf6N2E2_5476 at 6294.765 kb on + strand at 6294.766 kb on - strand at 6294.766 kb on - strand at 6295.041 kb on + strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.042 kb on - strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.045 kb on + strand at 6295.046 kb on - strand at 6295.053 kb on + strand at 6295.054 kb on - strand at 6295.306 kb on + strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.356 kb on - strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.363 kb on + strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.364 kb on - strand, within Pf6N2E2_5477 at 6295.499 kb on + strand, within Pf6N2E2_5477 at 6295.526 kb on + strand, within Pf6N2E2_5477 at 6295.537 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.562 kb on + strand, within Pf6N2E2_5477 at 6295.563 kb on - strand, within Pf6N2E2_5477 at 6295.799 kb on + strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.800 kb on - strand, within Pf6N2E2_5477 at 6295.841 kb on + strand, within Pf6N2E2_5477 at 6295.842 kb on - strand, within Pf6N2E2_5477 at 6295.881 kb on - strand, within Pf6N2E2_5477 at 6295.940 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.057 kb on + strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.058 kb on - strand, within Pf6N2E2_5477 at 6296.148 kb on - strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.348 kb on + strand at 6296.349 kb on - strand at 6296.491 kb on - strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.511 kb on + strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.512 kb on - strand, within Pf6N2E2_5478 at 6296.514 kb on + strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.515 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.596 kb on - strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.688 kb on + strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.689 kb on - strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.778 kb on + strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.779 kb on - strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.874 kb on + strand, within Pf6N2E2_5478 at 6296.985 kb on + strand, within Pf6N2E2_5478 at 6296.986 kb on - strand, within Pf6N2E2_5478 at 6297.153 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 6,294,406 - Pf6N2E2_5476 0.69 -0.9 6,294,765 + +1.0 6,294,766 - -0.0 6,294,766 - +0.3 6,295,041 + +0.5 6,295,042 - -1.1 6,295,042 - -0.2 6,295,042 - +0.5 6,295,045 + -1.1 6,295,045 + +2.2 6,295,045 + -1.5 6,295,046 - +0.2 6,295,053 + +0.4 6,295,054 - -1.9 6,295,306 + Pf6N2E2_5477 0.22 +1.1 6,295,356 - Pf6N2E2_5477 0.26 +0.7 6,295,356 - Pf6N2E2_5477 0.26 -0.8 6,295,363 + Pf6N2E2_5477 0.27 -3.1 6,295,363 + Pf6N2E2_5477 0.27 -2.4 6,295,364 - Pf6N2E2_5477 0.27 +0.2 6,295,364 - Pf6N2E2_5477 0.27 +1.3 6,295,364 - Pf6N2E2_5477 0.27 +1.4 6,295,499 + Pf6N2E2_5477 0.39 +1.1 6,295,526 + Pf6N2E2_5477 0.42 -0.8 6,295,537 + Pf6N2E2_5477 0.43 +1.2 6,295,562 + Pf6N2E2_5477 0.45 +0.2 6,295,562 + Pf6N2E2_5477 0.45 +2.2 6,295,563 - Pf6N2E2_5477 0.45 -0.0 6,295,799 + Pf6N2E2_5477 0.66 +1.0 6,295,800 - Pf6N2E2_5477 0.66 -1.6 6,295,800 - Pf6N2E2_5477 0.66 +0.2 6,295,841 + Pf6N2E2_5477 0.70 +1.2 6,295,842 - Pf6N2E2_5477 0.70 -0.3 6,295,881 - Pf6N2E2_5477 0.74 -2.9 6,295,940 + Pf6N2E2_5477 0.79 +1.5 6,296,057 + Pf6N2E2_5477 0.90 +0.7 6,296,057 + Pf6N2E2_5477 0.90 +0.2 6,296,057 + Pf6N2E2_5477 0.90 +0.2 6,296,057 + Pf6N2E2_5477 0.90 -0.8 6,296,057 + Pf6N2E2_5477 0.90 +0.0 6,296,057 + Pf6N2E2_5477 0.90 -0.8 6,296,058 - Pf6N2E2_5477 0.90 +0.9 6,296,058 - Pf6N2E2_5477 0.90 -0.8 6,296,058 - Pf6N2E2_5477 0.90 +0.7 6,296,058 - Pf6N2E2_5477 0.90 +0.8 6,296,058 - Pf6N2E2_5477 0.90 +0.7 6,296,148 - +0.8 6,296,348 + -0.6 6,296,348 + +0.5 6,296,348 + -1.6 6,296,348 + -3.4 6,296,349 - -1.1 6,296,491 - Pf6N2E2_5478 0.18 +1.5 6,296,511 + Pf6N2E2_5478 0.21 -0.5 6,296,511 + Pf6N2E2_5478 0.21 +0.4 6,296,511 + Pf6N2E2_5478 0.21 +0.2 6,296,512 - Pf6N2E2_5478 0.21 -0.8 6,296,512 - Pf6N2E2_5478 0.21 -1.1 6,296,512 - Pf6N2E2_5478 0.21 +0.0 6,296,512 - Pf6N2E2_5478 0.21 +0.3 6,296,514 + Pf6N2E2_5478 0.21 +0.1 6,296,515 - Pf6N2E2_5478 0.21 +0.6 6,296,515 - Pf6N2E2_5478 0.21 -1.1 6,296,596 - Pf6N2E2_5478 0.31 +0.2 6,296,596 - Pf6N2E2_5478 0.31 -0.8 6,296,688 + Pf6N2E2_5478 0.41 +0.2 6,296,688 + Pf6N2E2_5478 0.41 +0.1 6,296,689 - Pf6N2E2_5478 0.41 +0.2 6,296,689 - Pf6N2E2_5478 0.41 -0.8 6,296,778 + Pf6N2E2_5478 0.52 -0.1 6,296,778 + Pf6N2E2_5478 0.52 -0.2 6,296,778 + Pf6N2E2_5478 0.52 +0.4 6,296,778 + Pf6N2E2_5478 0.52 -0.9 6,296,778 + Pf6N2E2_5478 0.52 +0.2 6,296,779 - Pf6N2E2_5478 0.52 -0.9 6,296,779 - Pf6N2E2_5478 0.52 -0.5 6,296,779 - Pf6N2E2_5478 0.52 -2.1 6,296,874 + Pf6N2E2_5478 0.63 -0.1 6,296,874 + Pf6N2E2_5478 0.63 -0.7 6,296,874 + Pf6N2E2_5478 0.63 +2.3 6,296,874 + Pf6N2E2_5478 0.63 +0.4 6,296,874 + Pf6N2E2_5478 0.63 +1.2 6,296,874 + Pf6N2E2_5478 0.63 -0.4 6,296,985 + Pf6N2E2_5478 0.75 -2.2 6,296,986 - Pf6N2E2_5478 0.76 -2.2 6,297,153 + +2.2
Or see this region's nucleotide sequence