Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4815 and Pf6N2E2_4816 are separated by 49 nucleotides Pf6N2E2_4816 and Pf6N2E2_4817 are separated by 226 nucleotides
Pf6N2E2_4815 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 5,567,829 to 5,568,953
_4815
Pf6N2E2_4816 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 5,569,003 to 5,570,046
_4816
Pf6N2E2_4817 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 5,570,273 to 5,571,520
_4817
Position (kb)
5569
5570
5571 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.115 kb on - strand, within Pf6N2E2_4815 at 5568.296 kb on - strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.481 kb on + strand, within Pf6N2E2_4815 at 5568.528 kb on + strand, within Pf6N2E2_4815 at 5568.529 kb on - strand, within Pf6N2E2_4815 at 5568.529 kb on - strand, within Pf6N2E2_4815 at 5568.529 kb on - strand, within Pf6N2E2_4815 at 5568.529 kb on - strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.783 kb on + strand, within Pf6N2E2_4815 at 5568.784 kb on - strand, within Pf6N2E2_4815 at 5568.784 kb on - strand, within Pf6N2E2_4815 at 5568.784 kb on - strand, within Pf6N2E2_4815 at 5568.784 kb on - strand, within Pf6N2E2_4815 at 5568.940 kb on + strand at 5568.941 kb on - strand at 5568.941 kb on - strand at 5568.967 kb on + strand at 5568.967 kb on + strand at 5568.968 kb on - strand at 5568.968 kb on - strand at 5568.968 kb on - strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.000 kb on + strand at 5569.001 kb on - strand at 5569.001 kb on - strand at 5569.001 kb on - strand at 5569.001 kb on - strand at 5569.001 kb on - strand at 5569.001 kb on - strand at 5569.135 kb on + strand, within Pf6N2E2_4816 at 5569.135 kb on + strand, within Pf6N2E2_4816 at 5569.136 kb on - strand, within Pf6N2E2_4816 at 5569.136 kb on - strand, within Pf6N2E2_4816 at 5569.136 kb on - strand, within Pf6N2E2_4816 at 5569.183 kb on + strand, within Pf6N2E2_4816 at 5569.184 kb on - strand, within Pf6N2E2_4816 at 5569.184 kb on - strand, within Pf6N2E2_4816 at 5569.336 kb on + strand, within Pf6N2E2_4816 at 5569.337 kb on - strand, within Pf6N2E2_4816 at 5569.337 kb on - strand, within Pf6N2E2_4816 at 5569.399 kb on + strand, within Pf6N2E2_4816 at 5569.400 kb on - strand, within Pf6N2E2_4816 at 5569.498 kb on + strand, within Pf6N2E2_4816 at 5569.498 kb on + strand, within Pf6N2E2_4816 at 5569.498 kb on + strand, within Pf6N2E2_4816 at 5569.498 kb on + strand, within Pf6N2E2_4816 at 5569.498 kb on + strand, within Pf6N2E2_4816 at 5569.499 kb on - strand, within Pf6N2E2_4816 at 5569.549 kb on + strand, within Pf6N2E2_4816 at 5569.549 kb on + strand, within Pf6N2E2_4816 at 5569.549 kb on + strand, within Pf6N2E2_4816 at 5569.549 kb on + strand, within Pf6N2E2_4816 at 5569.550 kb on - strand, within Pf6N2E2_4816 at 5569.556 kb on + strand, within Pf6N2E2_4816 at 5569.752 kb on + strand, within Pf6N2E2_4816 at 5569.753 kb on - strand, within Pf6N2E2_4816 at 5569.756 kb on + strand, within Pf6N2E2_4816 at 5569.756 kb on + strand, within Pf6N2E2_4816 at 5569.757 kb on - strand, within Pf6N2E2_4816 at 5569.861 kb on + strand, within Pf6N2E2_4816 at 5569.861 kb on + strand, within Pf6N2E2_4816 at 5569.861 kb on + strand, within Pf6N2E2_4816 at 5569.861 kb on + strand, within Pf6N2E2_4816 at 5569.862 kb on - strand, within Pf6N2E2_4816 at 5569.862 kb on - strand, within Pf6N2E2_4816 at 5569.862 kb on - strand, within Pf6N2E2_4816 at 5569.879 kb on + strand, within Pf6N2E2_4816 at 5570.045 kb on - strand at 5570.045 kb on - strand at 5570.045 kb on - strand at 5570.243 kb on + strand at 5570.243 kb on + strand at 5570.531 kb on + strand, within Pf6N2E2_4817 at 5570.532 kb on - strand, within Pf6N2E2_4817 at 5570.681 kb on + strand, within Pf6N2E2_4817 at 5570.681 kb on + strand, within Pf6N2E2_4817 at 5570.681 kb on + strand, within Pf6N2E2_4817 at 5570.682 kb on - strand, within Pf6N2E2_4817 at 5570.682 kb on - strand, within Pf6N2E2_4817 at 5570.682 kb on - strand, within Pf6N2E2_4817 at 5570.682 kb on - strand, within Pf6N2E2_4817 at 5570.729 kb on + strand, within Pf6N2E2_4817 at 5570.771 kb on + strand, within Pf6N2E2_4817 at 5570.772 kb on - strand, within Pf6N2E2_4817 at 5570.774 kb on + strand, within Pf6N2E2_4817 at 5570.774 kb on + strand, within Pf6N2E2_4817 at 5570.774 kb on + strand, within Pf6N2E2_4817 at 5570.774 kb on + strand, within Pf6N2E2_4817 at 5570.775 kb on - strand, within Pf6N2E2_4817 at 5570.775 kb on - strand, within Pf6N2E2_4817 at 5570.831 kb on - strand, within Pf6N2E2_4817 at 5570.849 kb on + strand, within Pf6N2E2_4817 at 5570.849 kb on + strand, within Pf6N2E2_4817 at 5570.849 kb on + strand, within Pf6N2E2_4817 at 5570.849 kb on + strand, within Pf6N2E2_4817 at 5570.850 kb on - strand, within Pf6N2E2_4817 at 5570.850 kb on - strand, within Pf6N2E2_4817 at 5570.850 kb on - strand, within Pf6N2E2_4817 at 5570.850 kb on - strand, within Pf6N2E2_4817 at 5570.858 kb on - strand, within Pf6N2E2_4817 at 5570.918 kb on + strand, within Pf6N2E2_4817 at 5570.930 kb on + strand, within Pf6N2E2_4817
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 5,568,114 + Pf6N2E2_4815 0.25 -1.3 5,568,114 + Pf6N2E2_4815 0.25 -1.8 5,568,114 + Pf6N2E2_4815 0.25 +1.6 5,568,115 - Pf6N2E2_4815 0.25 -1.7 5,568,296 - Pf6N2E2_4815 0.42 -1.9 5,568,429 + Pf6N2E2_4815 0.53 -0.9 5,568,429 + Pf6N2E2_4815 0.53 -1.4 5,568,429 + Pf6N2E2_4815 0.53 -0.4 5,568,429 + Pf6N2E2_4815 0.53 -2.3 5,568,429 + Pf6N2E2_4815 0.53 -0.3 5,568,429 + Pf6N2E2_4815 0.53 -2.0 5,568,429 + Pf6N2E2_4815 0.53 -0.6 5,568,429 + Pf6N2E2_4815 0.53 -1.6 5,568,430 - Pf6N2E2_4815 0.53 -0.4 5,568,430 - Pf6N2E2_4815 0.53 -1.9 5,568,430 - Pf6N2E2_4815 0.53 -0.0 5,568,481 + Pf6N2E2_4815 0.58 -1.6 5,568,528 + Pf6N2E2_4815 0.62 -0.4 5,568,529 - Pf6N2E2_4815 0.62 -0.8 5,568,529 - Pf6N2E2_4815 0.62 -1.0 5,568,529 - Pf6N2E2_4815 0.62 +0.6 5,568,529 - Pf6N2E2_4815 0.62 -1.8 5,568,783 + Pf6N2E2_4815 0.85 -2.8 5,568,783 + Pf6N2E2_4815 0.85 -0.1 5,568,783 + Pf6N2E2_4815 0.85 +0.3 5,568,783 + Pf6N2E2_4815 0.85 -1.2 5,568,783 + Pf6N2E2_4815 0.85 -0.8 5,568,783 + Pf6N2E2_4815 0.85 +0.6 5,568,783 + Pf6N2E2_4815 0.85 -2.4 5,568,784 - Pf6N2E2_4815 0.85 +0.7 5,568,784 - Pf6N2E2_4815 0.85 -1.7 5,568,784 - Pf6N2E2_4815 0.85 +2.0 5,568,784 - Pf6N2E2_4815 0.85 -0.8 5,568,940 + -1.3 5,568,941 - -0.4 5,568,941 - +0.8 5,568,967 + +1.4 5,568,967 + +0.8 5,568,968 - +1.3 5,568,968 - +1.3 5,568,968 - +1.8 5,569,000 + +0.0 5,569,000 + -0.0 5,569,000 + -1.6 5,569,000 + -1.9 5,569,000 + +1.4 5,569,000 + -1.0 5,569,000 + +2.3 5,569,001 - -0.3 5,569,001 - +0.2 5,569,001 - -1.8 5,569,001 - +1.6 5,569,001 - -0.5 5,569,001 - -1.1 5,569,135 + Pf6N2E2_4816 0.13 -0.9 5,569,135 + Pf6N2E2_4816 0.13 +1.0 5,569,136 - Pf6N2E2_4816 0.13 -2.4 5,569,136 - Pf6N2E2_4816 0.13 -2.4 5,569,136 - Pf6N2E2_4816 0.13 +1.1 5,569,183 + Pf6N2E2_4816 0.17 +0.6 5,569,184 - Pf6N2E2_4816 0.17 -0.3 5,569,184 - Pf6N2E2_4816 0.17 -2.1 5,569,336 + Pf6N2E2_4816 0.32 +1.0 5,569,337 - Pf6N2E2_4816 0.32 -0.8 5,569,337 - Pf6N2E2_4816 0.32 -2.1 5,569,399 + Pf6N2E2_4816 0.38 +0.6 5,569,400 - Pf6N2E2_4816 0.38 +0.2 5,569,498 + Pf6N2E2_4816 0.47 +0.8 5,569,498 + Pf6N2E2_4816 0.47 -1.4 5,569,498 + Pf6N2E2_4816 0.47 -0.2 5,569,498 + Pf6N2E2_4816 0.47 +1.9 5,569,498 + Pf6N2E2_4816 0.47 -0.0 5,569,499 - Pf6N2E2_4816 0.48 -1.9 5,569,549 + Pf6N2E2_4816 0.52 +0.3 5,569,549 + Pf6N2E2_4816 0.52 +0.6 5,569,549 + Pf6N2E2_4816 0.52 -0.0 5,569,549 + Pf6N2E2_4816 0.52 +2.2 5,569,550 - Pf6N2E2_4816 0.52 +0.2 5,569,556 + Pf6N2E2_4816 0.53 -1.0 5,569,752 + Pf6N2E2_4816 0.72 +1.2 5,569,753 - Pf6N2E2_4816 0.72 -0.8 5,569,756 + Pf6N2E2_4816 0.72 +2.1 5,569,756 + Pf6N2E2_4816 0.72 -2.4 5,569,757 - Pf6N2E2_4816 0.72 -0.6 5,569,861 + Pf6N2E2_4816 0.82 +1.6 5,569,861 + Pf6N2E2_4816 0.82 -1.7 5,569,861 + Pf6N2E2_4816 0.82 +1.5 5,569,861 + Pf6N2E2_4816 0.82 +0.9 5,569,862 - Pf6N2E2_4816 0.82 +0.0 5,569,862 - Pf6N2E2_4816 0.82 +0.6 5,569,862 - Pf6N2E2_4816 0.82 -0.6 5,569,879 + Pf6N2E2_4816 0.84 -2.1 5,570,045 - -0.0 5,570,045 - -0.5 5,570,045 - -1.1 5,570,243 + -0.6 5,570,243 + -0.7 5,570,531 + Pf6N2E2_4817 0.21 -2.6 5,570,532 - Pf6N2E2_4817 0.21 -1.0 5,570,681 + Pf6N2E2_4817 0.33 -2.5 5,570,681 + Pf6N2E2_4817 0.33 -0.8 5,570,681 + Pf6N2E2_4817 0.33 -2.3 5,570,682 - Pf6N2E2_4817 0.33 -4.1 5,570,682 - Pf6N2E2_4817 0.33 -0.5 5,570,682 - Pf6N2E2_4817 0.33 -2.3 5,570,682 - Pf6N2E2_4817 0.33 -3.2 5,570,729 + Pf6N2E2_4817 0.37 -2.8 5,570,771 + Pf6N2E2_4817 0.40 -0.3 5,570,772 - Pf6N2E2_4817 0.40 -2.7 5,570,774 + Pf6N2E2_4817 0.40 -4.1 5,570,774 + Pf6N2E2_4817 0.40 -1.5 5,570,774 + Pf6N2E2_4817 0.40 -1.0 5,570,774 + Pf6N2E2_4817 0.40 -1.8 5,570,775 - Pf6N2E2_4817 0.40 -0.8 5,570,775 - Pf6N2E2_4817 0.40 -2.8 5,570,831 - Pf6N2E2_4817 0.45 -2.6 5,570,849 + Pf6N2E2_4817 0.46 +1.5 5,570,849 + Pf6N2E2_4817 0.46 -1.3 5,570,849 + Pf6N2E2_4817 0.46 -0.8 5,570,849 + Pf6N2E2_4817 0.46 -2.2 5,570,850 - Pf6N2E2_4817 0.46 -2.0 5,570,850 - Pf6N2E2_4817 0.46 +0.5 5,570,850 - Pf6N2E2_4817 0.46 -1.0 5,570,850 - Pf6N2E2_4817 0.46 -0.5 5,570,858 - Pf6N2E2_4817 0.47 -1.0 5,570,918 + Pf6N2E2_4817 0.52 -0.5 5,570,930 + Pf6N2E2_4817 0.53 -1.3
Or see this region's nucleotide sequence