Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4813 and Pf6N2E2_4814 are separated by 125 nucleotides Pf6N2E2_4814 and Pf6N2E2_4815 are separated by 348 nucleotides
Pf6N2E2_4813 - Probable two-component sensor, near polyamine transporter, at 5,564,329 to 5,566,725
_4813
Pf6N2E2_4814 - DNA-binding response regulator, LuxR family, near polyamine transporter, at 5,566,851 to 5,567,480
_4814
Pf6N2E2_4815 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 5,567,829 to 5,568,953
_4815
Position (kb) 5566
5567
5568 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5566.006 kb on + strand, within Pf6N2E2_4813 at 5566.006 kb on + strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.007 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.191 kb on - strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.251 kb on + strand, within Pf6N2E2_4813 at 5566.252 kb on - strand, within Pf6N2E2_4813 at 5566.252 kb on - strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.254 kb on + strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.255 kb on - strand, within Pf6N2E2_4813 at 5566.383 kb on + strand, within Pf6N2E2_4813 at 5566.509 kb on + strand at 5566.509 kb on + strand at 5566.510 kb on - strand at 5566.510 kb on - strand at 5566.514 kb on + strand at 5566.514 kb on + strand at 5566.515 kb on - strand at 5566.515 kb on - strand at 5566.515 kb on - strand at 5566.574 kb on + strand at 5566.575 kb on - strand at 5566.582 kb on - strand at 5566.590 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.788 kb on + strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.789 kb on - strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.794 kb on + strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.795 kb on - strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.861 kb on + strand at 5566.939 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.220 kb on + strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.221 kb on - strand, within Pf6N2E2_4814 at 5567.399 kb on + strand, within Pf6N2E2_4814 at 5567.399 kb on + strand, within Pf6N2E2_4814 at 5567.400 kb on - strand, within Pf6N2E2_4814 at 5567.454 kb on + strand at 5567.454 kb on + strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.455 kb on - strand at 5567.478 kb on + strand at 5567.479 kb on - strand at 5567.491 kb on + strand at 5567.492 kb on - strand at 5567.541 kb on + strand at 5567.541 kb on + strand at 5567.558 kb on - strand at 5567.606 kb on + strand at 5567.682 kb on + strand at 5567.682 kb on + strand at 5567.683 kb on - strand at 5567.704 kb on - strand at 5567.786 kb on + strand at 5567.786 kb on + strand at 5567.787 kb on - strand at 5567.813 kb on + strand at 5567.813 kb on + strand at 5567.813 kb on + strand at 5567.829 kb on - strand at 5567.898 kb on + strand at 5567.899 kb on - strand at 5567.899 kb on - strand at 5567.899 kb on - strand at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.114 kb on + strand, within Pf6N2E2_4815 at 5568.115 kb on - strand, within Pf6N2E2_4815 at 5568.296 kb on - strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.429 kb on + strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815 at 5568.430 kb on - strand, within Pf6N2E2_4815
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 5,566,006 + Pf6N2E2_4813 0.70 +0.7 5,566,006 + Pf6N2E2_4813 0.70 +0.6 5,566,007 - Pf6N2E2_4813 0.70 -1.7 5,566,007 - Pf6N2E2_4813 0.70 +1.3 5,566,007 - Pf6N2E2_4813 0.70 -0.3 5,566,007 - Pf6N2E2_4813 0.70 +1.6 5,566,007 - Pf6N2E2_4813 0.70 -1.3 5,566,007 - Pf6N2E2_4813 0.70 -0.3 5,566,007 - Pf6N2E2_4813 0.70 -2.8 5,566,191 - Pf6N2E2_4813 0.78 -0.4 5,566,191 - Pf6N2E2_4813 0.78 +1.3 5,566,191 - Pf6N2E2_4813 0.78 +0.6 5,566,191 - Pf6N2E2_4813 0.78 +0.7 5,566,191 - Pf6N2E2_4813 0.78 -0.0 5,566,251 + Pf6N2E2_4813 0.80 +0.0 5,566,251 + Pf6N2E2_4813 0.80 -3.2 5,566,251 + Pf6N2E2_4813 0.80 -0.7 5,566,251 + Pf6N2E2_4813 0.80 -0.9 5,566,252 - Pf6N2E2_4813 0.80 -0.0 5,566,252 - Pf6N2E2_4813 0.80 +0.1 5,566,254 + Pf6N2E2_4813 0.80 -2.5 5,566,254 + Pf6N2E2_4813 0.80 +0.0 5,566,254 + Pf6N2E2_4813 0.80 -0.8 5,566,255 - Pf6N2E2_4813 0.80 +0.6 5,566,255 - Pf6N2E2_4813 0.80 +0.1 5,566,255 - Pf6N2E2_4813 0.80 -2.1 5,566,255 - Pf6N2E2_4813 0.80 -1.9 5,566,255 - Pf6N2E2_4813 0.80 -1.0 5,566,255 - Pf6N2E2_4813 0.80 +1.4 5,566,383 + Pf6N2E2_4813 0.86 -0.3 5,566,509 + -0.4 5,566,509 + -1.5 5,566,510 - -1.2 5,566,510 - +1.1 5,566,514 + -1.8 5,566,514 + -1.3 5,566,515 - -1.3 5,566,515 - -0.9 5,566,515 - -1.3 5,566,574 + -0.5 5,566,575 - +0.5 5,566,582 - -1.9 5,566,590 + -2.5 5,566,788 + -0.2 5,566,788 + +1.0 5,566,788 + -1.0 5,566,788 + -1.1 5,566,788 + +0.7 5,566,788 + +0.5 5,566,788 + -1.4 5,566,788 + +0.8 5,566,788 + -1.7 5,566,789 - -0.4 5,566,789 - -1.4 5,566,789 - -1.0 5,566,789 - -1.9 5,566,789 - +0.3 5,566,789 - -1.7 5,566,789 - +0.7 5,566,789 - +1.6 5,566,789 - +1.2 5,566,789 - +0.7 5,566,789 - -1.3 5,566,789 - +0.8 5,566,789 - -0.6 5,566,789 - +0.6 5,566,789 - -1.1 5,566,794 + -0.4 5,566,794 + +0.6 5,566,794 + -1.2 5,566,794 + +0.4 5,566,794 + -0.6 5,566,794 + +0.0 5,566,794 + -0.8 5,566,794 + -0.2 5,566,794 + +2.4 5,566,794 + -0.0 5,566,794 + +0.4 5,566,794 + +1.2 5,566,794 + +0.5 5,566,794 + +0.3 5,566,794 + -0.4 5,566,795 - +0.0 5,566,795 - +0.4 5,566,795 - +0.6 5,566,795 - +0.6 5,566,795 - +0.7 5,566,795 - +1.5 5,566,795 - +2.2 5,566,795 - +0.9 5,566,795 - +1.3 5,566,795 - +2.0 5,566,795 - -1.3 5,566,861 + -0.8 5,566,861 + +0.0 5,566,861 + +0.3 5,566,861 + +0.1 5,566,939 + Pf6N2E2_4814 0.14 +0.1 5,567,220 + Pf6N2E2_4814 0.59 +0.9 5,567,220 + Pf6N2E2_4814 0.59 +0.4 5,567,220 + Pf6N2E2_4814 0.59 +0.1 5,567,220 + Pf6N2E2_4814 0.59 +0.2 5,567,220 + Pf6N2E2_4814 0.59 +0.1 5,567,221 - Pf6N2E2_4814 0.59 +0.9 5,567,221 - Pf6N2E2_4814 0.59 -2.3 5,567,221 - Pf6N2E2_4814 0.59 -0.4 5,567,221 - Pf6N2E2_4814 0.59 +1.8 5,567,221 - Pf6N2E2_4814 0.59 +0.7 5,567,399 + Pf6N2E2_4814 0.87 -1.0 5,567,399 + Pf6N2E2_4814 0.87 -2.6 5,567,400 - Pf6N2E2_4814 0.87 -2.4 5,567,454 + -0.8 5,567,454 + -0.3 5,567,455 - +0.2 5,567,455 - +1.2 5,567,455 - +0.3 5,567,455 - +2.6 5,567,455 - +0.6 5,567,455 - +1.0 5,567,478 + +0.7 5,567,479 - -3.4 5,567,491 + -2.5 5,567,492 - +0.2 5,567,541 + +1.4 5,567,541 + +1.8 5,567,558 - +0.6 5,567,606 + +0.1 5,567,682 + -0.8 5,567,682 + -1.4 5,567,683 - -0.6 5,567,704 - +0.2 5,567,786 + -0.8 5,567,786 + +0.4 5,567,787 - +0.4 5,567,813 + +0.0 5,567,813 + -1.8 5,567,813 + -1.6 5,567,829 - +1.7 5,567,898 + -0.0 5,567,899 - +0.1 5,567,899 - +0.1 5,567,899 - +0.8 5,568,114 + Pf6N2E2_4815 0.25 -1.3 5,568,114 + Pf6N2E2_4815 0.25 -1.8 5,568,114 + Pf6N2E2_4815 0.25 +1.6 5,568,115 - Pf6N2E2_4815 0.25 -1.7 5,568,296 - Pf6N2E2_4815 0.42 -1.9 5,568,429 + Pf6N2E2_4815 0.53 -0.9 5,568,429 + Pf6N2E2_4815 0.53 -1.4 5,568,429 + Pf6N2E2_4815 0.53 -0.4 5,568,429 + Pf6N2E2_4815 0.53 -2.3 5,568,429 + Pf6N2E2_4815 0.53 -0.3 5,568,429 + Pf6N2E2_4815 0.53 -2.0 5,568,429 + Pf6N2E2_4815 0.53 -0.6 5,568,429 + Pf6N2E2_4815 0.53 -1.6 5,568,430 - Pf6N2E2_4815 0.53 -0.4 5,568,430 - Pf6N2E2_4815 0.53 -1.9 5,568,430 - Pf6N2E2_4815 0.53 -0.0
Or see this region's nucleotide sequence