Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4455 and Pf6N2E2_4456 overlap by 14 nucleotides Pf6N2E2_4456 and Pf6N2E2_4457 overlap by 4 nucleotides Pf6N2E2_4457 and Pf6N2E2_4458 are separated by 21 nucleotides
Pf6N2E2_4455 - Glutamate transport ATP-binding protein, at 5,188,174 to 5,188,908
_4455
Pf6N2E2_4456 - Putative amino acid ABC transporter, permease protein, at 5,188,895 to 5,189,545
_4456
Pf6N2E2_4457 - amino acid ABC transporter, permease protein, at 5,189,542 to 5,190,222
_4457
Pf6N2E2_4458 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 5,190,244 to 5,191,029
_4458
Position (kb)
5189
5190
5191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.591 kb on + strand, within Pf6N2E2_4455 at 5188.592 kb on - strand, within Pf6N2E2_4455 at 5188.592 kb on - strand, within Pf6N2E2_4455 at 5188.712 kb on + strand, within Pf6N2E2_4455 at 5188.712 kb on + strand, within Pf6N2E2_4455 at 5188.713 kb on - strand, within Pf6N2E2_4455 at 5188.872 kb on - strand at 5188.872 kb on - strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.874 kb on + strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.875 kb on - strand at 5188.878 kb on + strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.879 kb on - strand at 5188.896 kb on + strand at 5188.896 kb on + strand at 5188.896 kb on + strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.897 kb on - strand at 5188.965 kb on + strand, within Pf6N2E2_4456 at 5188.965 kb on + strand, within Pf6N2E2_4456 at 5188.966 kb on - strand, within Pf6N2E2_4456 at 5188.986 kb on + strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5188.987 kb on - strand, within Pf6N2E2_4456 at 5189.149 kb on - strand, within Pf6N2E2_4456 at 5189.248 kb on - strand, within Pf6N2E2_4456 at 5189.258 kb on - strand, within Pf6N2E2_4456 at 5189.258 kb on - strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.379 kb on + strand, within Pf6N2E2_4456 at 5189.380 kb on - strand, within Pf6N2E2_4456 at 5189.380 kb on - strand, within Pf6N2E2_4456 at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.570 kb on + strand at 5189.571 kb on - strand at 5189.630 kb on + strand, within Pf6N2E2_4457 at 5189.630 kb on + strand, within Pf6N2E2_4457 at 5189.631 kb on - strand, within Pf6N2E2_4457 at 5189.651 kb on + strand, within Pf6N2E2_4457 at 5189.652 kb on - strand, within Pf6N2E2_4457 at 5189.652 kb on - strand, within Pf6N2E2_4457 at 5189.673 kb on + strand, within Pf6N2E2_4457 at 5189.673 kb on + strand, within Pf6N2E2_4457 at 5189.674 kb on - strand, within Pf6N2E2_4457 at 5189.912 kb on + strand, within Pf6N2E2_4457 at 5189.912 kb on + strand, within Pf6N2E2_4457 at 5190.044 kb on + strand, within Pf6N2E2_4457 at 5190.045 kb on - strand, within Pf6N2E2_4457 at 5190.045 kb on - strand, within Pf6N2E2_4457 at 5190.129 kb on + strand, within Pf6N2E2_4457 at 5190.129 kb on + strand, within Pf6N2E2_4457 at 5190.130 kb on - strand, within Pf6N2E2_4457 at 5190.130 kb on - strand, within Pf6N2E2_4457 at 5190.130 kb on - strand, within Pf6N2E2_4457 at 5190.215 kb on + strand at 5190.216 kb on - strand at 5190.216 kb on - strand at 5190.377 kb on + strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.378 kb on - strand, within Pf6N2E2_4458 at 5190.494 kb on + strand, within Pf6N2E2_4458 at 5190.495 kb on - strand, within Pf6N2E2_4458 at 5190.665 kb on + strand, within Pf6N2E2_4458 at 5190.666 kb on - strand, within Pf6N2E2_4458 at 5190.666 kb on - strand, within Pf6N2E2_4458 at 5190.666 kb on - strand, within Pf6N2E2_4458 at 5190.677 kb on + strand, within Pf6N2E2_4458 at 5191.190 kb on + strand at 5191.191 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 5,188,591 + Pf6N2E2_4455 0.57 +1.2 5,188,591 + Pf6N2E2_4455 0.57 +1.1 5,188,591 + Pf6N2E2_4455 0.57 +0.7 5,188,592 - Pf6N2E2_4455 0.57 +1.0 5,188,592 - Pf6N2E2_4455 0.57 +2.0 5,188,712 + Pf6N2E2_4455 0.73 -0.6 5,188,712 + Pf6N2E2_4455 0.73 +0.6 5,188,713 - Pf6N2E2_4455 0.73 +1.1 5,188,872 - -1.0 5,188,872 - -2.2 5,188,874 + -0.6 5,188,874 + -0.3 5,188,874 + -3.1 5,188,874 + +0.7 5,188,875 - -2.6 5,188,875 - +0.0 5,188,875 - +0.6 5,188,875 - +0.3 5,188,875 - -1.0 5,188,875 - +0.7 5,188,878 + -0.7 5,188,879 - +1.8 5,188,879 - -2.2 5,188,879 - +0.7 5,188,879 - +1.8 5,188,896 + +1.0 5,188,896 + -1.5 5,188,896 + +0.5 5,188,897 - -2.3 5,188,897 - -0.5 5,188,897 - -0.8 5,188,897 - +0.1 5,188,965 + Pf6N2E2_4456 0.11 +0.5 5,188,965 + Pf6N2E2_4456 0.11 +0.1 5,188,966 - Pf6N2E2_4456 0.11 -1.0 5,188,986 + Pf6N2E2_4456 0.14 +0.5 5,188,987 - Pf6N2E2_4456 0.14 -0.2 5,188,987 - Pf6N2E2_4456 0.14 +0.1 5,188,987 - Pf6N2E2_4456 0.14 -1.0 5,189,149 - Pf6N2E2_4456 0.39 +0.0 5,189,248 - Pf6N2E2_4456 0.54 +0.4 5,189,258 - Pf6N2E2_4456 0.56 -0.2 5,189,258 - Pf6N2E2_4456 0.56 +0.5 5,189,379 + Pf6N2E2_4456 0.74 +0.6 5,189,379 + Pf6N2E2_4456 0.74 +0.9 5,189,379 + Pf6N2E2_4456 0.74 -0.9 5,189,379 + Pf6N2E2_4456 0.74 +1.2 5,189,379 + Pf6N2E2_4456 0.74 -0.3 5,189,380 - Pf6N2E2_4456 0.75 +1.7 5,189,380 - Pf6N2E2_4456 0.75 +0.6 5,189,570 + -0.3 5,189,570 + -1.1 5,189,570 + -0.5 5,189,570 + -2.6 5,189,571 - +0.9 5,189,630 + Pf6N2E2_4457 0.13 -1.2 5,189,630 + Pf6N2E2_4457 0.13 +0.8 5,189,631 - Pf6N2E2_4457 0.13 +0.6 5,189,651 + Pf6N2E2_4457 0.16 +0.6 5,189,652 - Pf6N2E2_4457 0.16 +0.9 5,189,652 - Pf6N2E2_4457 0.16 -1.0 5,189,673 + Pf6N2E2_4457 0.19 -0.3 5,189,673 + Pf6N2E2_4457 0.19 -2.2 5,189,674 - Pf6N2E2_4457 0.19 -3.5 5,189,912 + Pf6N2E2_4457 0.54 +0.6 5,189,912 + Pf6N2E2_4457 0.54 -0.8 5,190,044 + Pf6N2E2_4457 0.74 -0.8 5,190,045 - Pf6N2E2_4457 0.74 -0.9 5,190,045 - Pf6N2E2_4457 0.74 +0.1 5,190,129 + Pf6N2E2_4457 0.86 -1.4 5,190,129 + Pf6N2E2_4457 0.86 -1.0 5,190,130 - Pf6N2E2_4457 0.86 +0.1 5,190,130 - Pf6N2E2_4457 0.86 -1.0 5,190,130 - Pf6N2E2_4457 0.86 -0.6 5,190,215 + +0.7 5,190,216 - -2.3 5,190,216 - +0.1 5,190,377 + Pf6N2E2_4458 0.17 -0.8 5,190,378 - Pf6N2E2_4458 0.17 -1.3 5,190,378 - Pf6N2E2_4458 0.17 -0.6 5,190,378 - Pf6N2E2_4458 0.17 +0.7 5,190,378 - Pf6N2E2_4458 0.17 -1.2 5,190,378 - Pf6N2E2_4458 0.17 -1.5 5,190,378 - Pf6N2E2_4458 0.17 +0.9 5,190,494 + Pf6N2E2_4458 0.32 +0.3 5,190,495 - Pf6N2E2_4458 0.32 -0.2 5,190,665 + Pf6N2E2_4458 0.54 -0.7 5,190,666 - Pf6N2E2_4458 0.54 -0.4 5,190,666 - Pf6N2E2_4458 0.54 -2.5 5,190,666 - Pf6N2E2_4458 0.54 +1.0 5,190,677 + Pf6N2E2_4458 0.55 -2.2 5,191,190 + +0.0 5,191,191 - -0.8
Or see this region's nucleotide sequence