Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4245 and Pf6N2E2_4246 are separated by 95 nucleotides Pf6N2E2_4246 and Pf6N2E2_4247 overlap by 4 nucleotides Pf6N2E2_4247 and Pf6N2E2_4248 overlap by 7 nucleotides Pf6N2E2_4248 and Pf6N2E2_4249 are separated by 153 nucleotides
Pf6N2E2_4245 - Glycosyltransferase (EC 2.4.1.-), at 4,938,475 to 4,939,767
_4245
Pf6N2E2_4246 - SAM-dependent methyltransferases, at 4,939,863 to 4,940,636
_4246
Pf6N2E2_4247 - O-antigen acetylase, at 4,940,633 to 4,941,787
_4247
Pf6N2E2_4248 - SAM-dependent methyltransferases, at 4,941,781 to 4,942,605
_4248
Pf6N2E2_4249 - FIG01055313: hypothetical protein, at 4,942,759 to 4,943,952
_4249
Position (kb)
4940
4941
4942 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4939.713 kb on - strand at 4939.733 kb on + strand at 4939.734 kb on - strand at 4939.734 kb on - strand at 4939.734 kb on - strand at 4939.855 kb on - strand at 4940.159 kb on + strand, within Pf6N2E2_4246 at 4940.160 kb on - strand, within Pf6N2E2_4246 at 4940.160 kb on - strand, within Pf6N2E2_4246 at 4940.220 kb on + strand, within Pf6N2E2_4246 at 4940.220 kb on + strand, within Pf6N2E2_4246 at 4940.221 kb on - strand, within Pf6N2E2_4246 at 4940.221 kb on - strand, within Pf6N2E2_4246 at 4940.221 kb on - strand, within Pf6N2E2_4246 at 4940.221 kb on - strand, within Pf6N2E2_4246 at 4940.221 kb on - strand, within Pf6N2E2_4246 at 4940.284 kb on - strand, within Pf6N2E2_4246 at 4940.603 kb on + strand at 4940.821 kb on + strand, within Pf6N2E2_4247 at 4940.821 kb on + strand, within Pf6N2E2_4247 at 4940.821 kb on + strand, within Pf6N2E2_4247 at 4940.822 kb on - strand, within Pf6N2E2_4247 at 4940.822 kb on - strand, within Pf6N2E2_4247 at 4941.068 kb on + strand, within Pf6N2E2_4247 at 4941.068 kb on + strand, within Pf6N2E2_4247 at 4941.069 kb on - strand, within Pf6N2E2_4247 at 4941.069 kb on - strand, within Pf6N2E2_4247 at 4941.069 kb on - strand, within Pf6N2E2_4247 at 4941.069 kb on - strand, within Pf6N2E2_4247 at 4941.074 kb on + strand, within Pf6N2E2_4247 at 4941.075 kb on - strand, within Pf6N2E2_4247 at 4941.122 kb on + strand, within Pf6N2E2_4247 at 4941.122 kb on + strand, within Pf6N2E2_4247 at 4941.123 kb on - strand, within Pf6N2E2_4247 at 4941.123 kb on - strand, within Pf6N2E2_4247 at 4941.123 kb on - strand, within Pf6N2E2_4247 at 4941.123 kb on - strand, within Pf6N2E2_4247 at 4941.133 kb on - strand, within Pf6N2E2_4247 at 4941.161 kb on + strand, within Pf6N2E2_4247 at 4941.294 kb on - strand, within Pf6N2E2_4247 at 4941.407 kb on + strand, within Pf6N2E2_4247 at 4941.407 kb on + strand, within Pf6N2E2_4247 at 4941.407 kb on + strand, within Pf6N2E2_4247 at 4941.408 kb on - strand, within Pf6N2E2_4247 at 4941.408 kb on - strand, within Pf6N2E2_4247 at 4941.408 kb on - strand, within Pf6N2E2_4247 at 4941.408 kb on - strand, within Pf6N2E2_4247 at 4941.461 kb on + strand, within Pf6N2E2_4247 at 4941.461 kb on + strand, within Pf6N2E2_4247 at 4941.461 kb on + strand, within Pf6N2E2_4247 at 4941.461 kb on + strand, within Pf6N2E2_4247 at 4941.462 kb on - strand, within Pf6N2E2_4247 at 4941.462 kb on - strand, within Pf6N2E2_4247 at 4941.473 kb on + strand, within Pf6N2E2_4247 at 4941.473 kb on + strand, within Pf6N2E2_4247 at 4941.473 kb on + strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.474 kb on - strand, within Pf6N2E2_4247 at 4941.497 kb on - strand, within Pf6N2E2_4247 at 4941.539 kb on + strand, within Pf6N2E2_4247 at 4941.539 kb on + strand, within Pf6N2E2_4247 at 4941.539 kb on + strand, within Pf6N2E2_4247 at 4941.540 kb on - strand, within Pf6N2E2_4247 at 4941.540 kb on - strand, within Pf6N2E2_4247 at 4941.540 kb on - strand, within Pf6N2E2_4247 at 4941.540 kb on - strand, within Pf6N2E2_4247 at 4941.540 kb on - strand, within Pf6N2E2_4247 at 4941.683 kb on + strand at 4941.683 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.856 kb on + strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4941.857 kb on - strand at 4942.212 kb on - strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.237 kb on + strand, within Pf6N2E2_4248 at 4942.238 kb on - strand, within Pf6N2E2_4248 at 4942.301 kb on - strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.374 kb on + strand, within Pf6N2E2_4248 at 4942.375 kb on - strand, within Pf6N2E2_4248 at 4942.396 kb on + strand, within Pf6N2E2_4248 at 4942.406 kb on - strand, within Pf6N2E2_4248 at 4942.406 kb on - strand, within Pf6N2E2_4248 at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.576 kb on - strand at 4942.585 kb on - strand at 4942.604 kb on - strand at 4942.727 kb on + strand at 4942.742 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 4,939,713 - -0.0 4,939,733 + +0.9 4,939,734 - +1.5 4,939,734 - -2.2 4,939,734 - +1.4 4,939,855 - -1.4 4,940,159 + Pf6N2E2_4246 0.38 +0.3 4,940,160 - Pf6N2E2_4246 0.38 -2.3 4,940,160 - Pf6N2E2_4246 0.38 -3.6 4,940,220 + Pf6N2E2_4246 0.46 +0.8 4,940,220 + Pf6N2E2_4246 0.46 +0.7 4,940,221 - Pf6N2E2_4246 0.46 -1.0 4,940,221 - Pf6N2E2_4246 0.46 -0.9 4,940,221 - Pf6N2E2_4246 0.46 -1.9 4,940,221 - Pf6N2E2_4246 0.46 -1.4 4,940,221 - Pf6N2E2_4246 0.46 +0.2 4,940,284 - Pf6N2E2_4246 0.54 -2.6 4,940,603 + +0.4 4,940,821 + Pf6N2E2_4247 0.16 -1.6 4,940,821 + Pf6N2E2_4247 0.16 +0.7 4,940,821 + Pf6N2E2_4247 0.16 +0.2 4,940,822 - Pf6N2E2_4247 0.16 -2.9 4,940,822 - Pf6N2E2_4247 0.16 -1.3 4,941,068 + Pf6N2E2_4247 0.38 -2.1 4,941,068 + Pf6N2E2_4247 0.38 +0.4 4,941,069 - Pf6N2E2_4247 0.38 -2.8 4,941,069 - Pf6N2E2_4247 0.38 -0.1 4,941,069 - Pf6N2E2_4247 0.38 -0.5 4,941,069 - Pf6N2E2_4247 0.38 -0.2 4,941,074 + Pf6N2E2_4247 0.38 +2.6 4,941,075 - Pf6N2E2_4247 0.38 -2.2 4,941,122 + Pf6N2E2_4247 0.42 -1.0 4,941,122 + Pf6N2E2_4247 0.42 +1.8 4,941,123 - Pf6N2E2_4247 0.42 -1.1 4,941,123 - Pf6N2E2_4247 0.42 -1.2 4,941,123 - Pf6N2E2_4247 0.42 +1.8 4,941,123 - Pf6N2E2_4247 0.42 -1.3 4,941,133 - Pf6N2E2_4247 0.43 -1.3 4,941,161 + Pf6N2E2_4247 0.46 -0.8 4,941,294 - Pf6N2E2_4247 0.57 -0.7 4,941,407 + Pf6N2E2_4247 0.67 -2.4 4,941,407 + Pf6N2E2_4247 0.67 +0.3 4,941,407 + Pf6N2E2_4247 0.67 -0.0 4,941,408 - Pf6N2E2_4247 0.67 +1.0 4,941,408 - Pf6N2E2_4247 0.67 +0.7 4,941,408 - Pf6N2E2_4247 0.67 +1.1 4,941,408 - Pf6N2E2_4247 0.67 -0.4 4,941,461 + Pf6N2E2_4247 0.72 +0.2 4,941,461 + Pf6N2E2_4247 0.72 -0.9 4,941,461 + Pf6N2E2_4247 0.72 -0.5 4,941,461 + Pf6N2E2_4247 0.72 -0.2 4,941,462 - Pf6N2E2_4247 0.72 -1.0 4,941,462 - Pf6N2E2_4247 0.72 -0.7 4,941,473 + Pf6N2E2_4247 0.73 -0.0 4,941,473 + Pf6N2E2_4247 0.73 -2.3 4,941,473 + Pf6N2E2_4247 0.73 -1.0 4,941,474 - Pf6N2E2_4247 0.73 -0.7 4,941,474 - Pf6N2E2_4247 0.73 +1.0 4,941,474 - Pf6N2E2_4247 0.73 -0.8 4,941,474 - Pf6N2E2_4247 0.73 -0.2 4,941,474 - Pf6N2E2_4247 0.73 -1.2 4,941,474 - Pf6N2E2_4247 0.73 +0.8 4,941,474 - Pf6N2E2_4247 0.73 -0.8 4,941,497 - Pf6N2E2_4247 0.75 +0.2 4,941,539 + Pf6N2E2_4247 0.78 -1.5 4,941,539 + Pf6N2E2_4247 0.78 -1.2 4,941,539 + Pf6N2E2_4247 0.78 +0.4 4,941,540 - Pf6N2E2_4247 0.79 +1.5 4,941,540 - Pf6N2E2_4247 0.79 +0.2 4,941,540 - Pf6N2E2_4247 0.79 -1.9 4,941,540 - Pf6N2E2_4247 0.79 -0.2 4,941,540 - Pf6N2E2_4247 0.79 +0.8 4,941,683 + +0.1 4,941,683 + +0.0 4,941,856 + -0.8 4,941,856 + +1.5 4,941,856 + +0.5 4,941,856 + +0.0 4,941,856 + -0.9 4,941,856 + -2.0 4,941,857 - +1.1 4,941,857 - -2.1 4,941,857 - +1.8 4,941,857 - +0.6 4,941,857 - -0.8 4,941,857 - +0.8 4,941,857 - +0.7 4,941,857 - -2.3 4,941,857 - -0.1 4,941,857 - -1.0 4,942,212 - Pf6N2E2_4248 0.52 -1.0 4,942,237 + Pf6N2E2_4248 0.55 -0.6 4,942,237 + Pf6N2E2_4248 0.55 -0.2 4,942,237 + Pf6N2E2_4248 0.55 +0.8 4,942,237 + Pf6N2E2_4248 0.55 -2.6 4,942,238 - Pf6N2E2_4248 0.55 -2.1 4,942,301 - Pf6N2E2_4248 0.63 -0.5 4,942,374 + Pf6N2E2_4248 0.72 +0.9 4,942,374 + Pf6N2E2_4248 0.72 -0.4 4,942,374 + Pf6N2E2_4248 0.72 -1.2 4,942,374 + Pf6N2E2_4248 0.72 -0.4 4,942,375 - Pf6N2E2_4248 0.72 +1.8 4,942,396 + Pf6N2E2_4248 0.75 -2.0 4,942,406 - Pf6N2E2_4248 0.76 -0.0 4,942,406 - Pf6N2E2_4248 0.76 +0.2 4,942,576 - -2.2 4,942,576 - +0.2 4,942,576 - -0.1 4,942,576 - -0.8 4,942,585 - +1.8 4,942,604 - +0.8 4,942,727 + -0.0 4,942,742 + -2.1
Or see this region's nucleotide sequence