Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4190 and Pf6N2E2_4191 are separated by 10 nucleotides Pf6N2E2_4191 and Pf6N2E2_4192 are separated by 80 nucleotides Pf6N2E2_4192 and Pf6N2E2_4193 are separated by 177 nucleotides
Pf6N2E2_4190 - Glycyl-tRNA synthetase beta chain (EC 6.1.1.14), at 4,874,822 to 4,876,873
_4190
Pf6N2E2_4191 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-); Histidinol-phosphatase (EC 3.1.3.15), at 4,876,884 to 4,877,417
_4191
Pf6N2E2_4192 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 4,877,498 to 4,878,277
_4192
Pf6N2E2_4193 - Signal transduction histidine kinase, at 4,878,455 to 4,879,972
_4193
Position (kb)
4877
4878
4879 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 4877.415 kb on + strand at 4877.415 kb on + strand at 4877.497 kb on + strand at 4877.497 kb on + strand at 4877.633 kb on + strand at 4878.138 kb on - strand at 4878.389 kb on - strand at 4878.389 kb on - strand at 4878.389 kb on - strand at 4878.484 kb on + strand at 4878.511 kb on + strand at 4878.512 kb on - strand at 4878.620 kb on - strand, within Pf6N2E2_4193 at 4878.620 kb on - strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.632 kb on + strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.633 kb on - strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.735 kb on + strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.736 kb on - strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.818 kb on + strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.819 kb on - strand, within Pf6N2E2_4193 at 4878.918 kb on + strand, within Pf6N2E2_4193 at 4878.918 kb on + strand, within Pf6N2E2_4193 at 4878.980 kb on + strand, within Pf6N2E2_4193 at 4878.980 kb on + strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4878.981 kb on - strand, within Pf6N2E2_4193 at 4879.013 kb on - strand, within Pf6N2E2_4193
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 4,877,415 + -1.1 4,877,415 + -3.4 4,877,497 + -5.1 4,877,497 + -4.8 4,877,633 + -2.8 4,878,138 - -2.3 4,878,389 - -0.8 4,878,389 - -1.2 4,878,389 - -0.8 4,878,484 + -2.2 4,878,511 + -0.5 4,878,512 - +1.2 4,878,620 - Pf6N2E2_4193 0.11 -1.0 4,878,620 - Pf6N2E2_4193 0.11 +0.9 4,878,632 + Pf6N2E2_4193 0.12 -0.2 4,878,632 + Pf6N2E2_4193 0.12 +1.1 4,878,632 + Pf6N2E2_4193 0.12 -0.1 4,878,632 + Pf6N2E2_4193 0.12 +0.3 4,878,633 - Pf6N2E2_4193 0.12 +0.2 4,878,633 - Pf6N2E2_4193 0.12 +0.7 4,878,633 - Pf6N2E2_4193 0.12 +0.2 4,878,633 - Pf6N2E2_4193 0.12 +1.5 4,878,633 - Pf6N2E2_4193 0.12 +1.0 4,878,735 + Pf6N2E2_4193 0.18 +0.7 4,878,735 + Pf6N2E2_4193 0.18 -0.8 4,878,735 + Pf6N2E2_4193 0.18 +1.6 4,878,735 + Pf6N2E2_4193 0.18 -0.0 4,878,736 - Pf6N2E2_4193 0.19 +0.8 4,878,736 - Pf6N2E2_4193 0.19 -0.5 4,878,736 - Pf6N2E2_4193 0.19 +0.1 4,878,736 - Pf6N2E2_4193 0.19 -1.0 4,878,736 - Pf6N2E2_4193 0.19 -0.3 4,878,736 - Pf6N2E2_4193 0.19 +0.4 4,878,736 - Pf6N2E2_4193 0.19 -1.2 4,878,736 - Pf6N2E2_4193 0.19 -0.3 4,878,736 - Pf6N2E2_4193 0.19 +0.4 4,878,736 - Pf6N2E2_4193 0.19 -0.2 4,878,736 - Pf6N2E2_4193 0.19 +0.2 4,878,736 - Pf6N2E2_4193 0.19 -1.3 4,878,818 + Pf6N2E2_4193 0.24 +1.5 4,878,818 + Pf6N2E2_4193 0.24 +0.2 4,878,818 + Pf6N2E2_4193 0.24 -1.2 4,878,818 + Pf6N2E2_4193 0.24 -0.1 4,878,818 + Pf6N2E2_4193 0.24 +0.4 4,878,819 - Pf6N2E2_4193 0.24 +0.1 4,878,819 - Pf6N2E2_4193 0.24 +1.9 4,878,819 - Pf6N2E2_4193 0.24 -1.0 4,878,918 + Pf6N2E2_4193 0.31 +1.6 4,878,918 + Pf6N2E2_4193 0.31 +0.3 4,878,980 + Pf6N2E2_4193 0.35 +0.2 4,878,980 + Pf6N2E2_4193 0.35 +0.1 4,878,981 - Pf6N2E2_4193 0.35 -0.2 4,878,981 - Pf6N2E2_4193 0.35 -0.7 4,878,981 - Pf6N2E2_4193 0.35 +0.0 4,879,013 - Pf6N2E2_4193 0.37 +1.2
Or see this region's nucleotide sequence