Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4177 and Pf6N2E2_4178 are separated by 148 nucleotides Pf6N2E2_4178 and Pf6N2E2_4179 are separated by 150 nucleotides Pf6N2E2_4179 and Pf6N2E2_4180 are separated by 208 nucleotides
Pf6N2E2_4177 - hypothetical protein, at 4,862,475 to 4,862,732
_4177
Pf6N2E2_4178 - Glutathione S-transferase (EC 2.5.1.18), at 4,862,881 to 4,863,576
_4178
Pf6N2E2_4179 - Transcriptional regulator, TetR family, at 4,863,727 to 4,864,290
_4179
Pf6N2E2_4180 - FIG00955126: hypothetical protein, at 4,864,499 to 4,865,623
_4180
Position (kb)
4863
4864
4865 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4862.738 kb on - strand at 4862.882 kb on + strand at 4862.882 kb on + strand at 4862.883 kb on - strand at 4862.883 kb on - strand at 4863.023 kb on - strand, within Pf6N2E2_4178 at 4863.092 kb on + strand, within Pf6N2E2_4178 at 4863.093 kb on - strand, within Pf6N2E2_4178 at 4863.093 kb on - strand, within Pf6N2E2_4178 at 4863.093 kb on - strand, within Pf6N2E2_4178 at 4863.093 kb on - strand, within Pf6N2E2_4178 at 4863.093 kb on - strand, within Pf6N2E2_4178 at 4863.149 kb on + strand, within Pf6N2E2_4178 at 4863.149 kb on + strand, within Pf6N2E2_4178 at 4863.150 kb on - strand, within Pf6N2E2_4178 at 4863.194 kb on + strand, within Pf6N2E2_4178 at 4863.243 kb on + strand, within Pf6N2E2_4178 at 4863.243 kb on + strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.244 kb on - strand, within Pf6N2E2_4178 at 4863.311 kb on + strand, within Pf6N2E2_4178 at 4863.311 kb on + strand, within Pf6N2E2_4178 at 4863.383 kb on + strand, within Pf6N2E2_4178 at 4863.384 kb on - strand, within Pf6N2E2_4178 at 4863.384 kb on - strand, within Pf6N2E2_4178 at 4863.384 kb on - strand, within Pf6N2E2_4178 at 4863.384 kb on - strand, within Pf6N2E2_4178 at 4863.522 kb on - strand at 4863.522 kb on - strand at 4863.534 kb on + strand at 4863.535 kb on - strand at 4863.535 kb on - strand at 4863.677 kb on + strand at 4863.702 kb on - strand at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.850 kb on + strand, within Pf6N2E2_4179 at 4863.851 kb on - strand, within Pf6N2E2_4179 at 4863.851 kb on - strand, within Pf6N2E2_4179 at 4863.853 kb on + strand, within Pf6N2E2_4179 at 4863.936 kb on - strand, within Pf6N2E2_4179 at 4863.976 kb on + strand, within Pf6N2E2_4179 at 4863.976 kb on + strand, within Pf6N2E2_4179 at 4863.977 kb on - strand, within Pf6N2E2_4179 at 4863.977 kb on - strand, within Pf6N2E2_4179 at 4864.048 kb on + strand, within Pf6N2E2_4179 at 4864.048 kb on + strand, within Pf6N2E2_4179 at 4864.048 kb on + strand, within Pf6N2E2_4179 at 4864.070 kb on + strand, within Pf6N2E2_4179 at 4864.071 kb on - strand, within Pf6N2E2_4179 at 4864.071 kb on - strand, within Pf6N2E2_4179 at 4864.071 kb on - strand, within Pf6N2E2_4179 at 4864.124 kb on + strand, within Pf6N2E2_4179 at 4864.147 kb on - strand, within Pf6N2E2_4179 at 4864.147 kb on - strand, within Pf6N2E2_4179 at 4864.183 kb on + strand, within Pf6N2E2_4179 at 4864.183 kb on + strand, within Pf6N2E2_4179 at 4864.183 kb on + strand, within Pf6N2E2_4179 at 4864.184 kb on - strand, within Pf6N2E2_4179 at 4864.184 kb on - strand, within Pf6N2E2_4179 at 4864.185 kb on + strand, within Pf6N2E2_4179 at 4864.208 kb on + strand, within Pf6N2E2_4179 at 4864.209 kb on - strand, within Pf6N2E2_4179 at 4864.209 kb on - strand, within Pf6N2E2_4179 at 4864.209 kb on - strand, within Pf6N2E2_4179 at 4864.253 kb on + strand at 4864.253 kb on + strand at 4864.261 kb on + strand at 4864.396 kb on + strand at 4864.409 kb on + strand at 4864.410 kb on - strand at 4864.427 kb on + strand at 4864.428 kb on - strand at 4864.502 kb on + strand at 4864.502 kb on + strand at 4864.502 kb on + strand at 4864.503 kb on - strand at 4864.503 kb on - strand at 4864.536 kb on + strand at 4864.536 kb on + strand at 4864.536 kb on + strand at 4864.536 kb on + strand at 4864.536 kb on + strand at 4864.606 kb on + strand at 4864.607 kb on - strand at 4864.607 kb on - strand at 4864.607 kb on - strand at 4864.607 kb on - strand at 4864.610 kb on + strand at 4864.610 kb on + strand at 4864.611 kb on - strand at 4864.611 kb on - strand at 4864.611 kb on - strand at 4864.611 kb on - strand at 4864.611 kb on - strand at 4864.611 kb on - strand at 4864.781 kb on + strand, within Pf6N2E2_4180 at 4864.782 kb on - strand, within Pf6N2E2_4180 at 4864.801 kb on - strand, within Pf6N2E2_4180 at 4864.810 kb on + strand, within Pf6N2E2_4180 at 4864.810 kb on + strand, within Pf6N2E2_4180 at 4864.810 kb on + strand, within Pf6N2E2_4180 at 4864.882 kb on + strand, within Pf6N2E2_4180 at 4864.882 kb on + strand, within Pf6N2E2_4180 at 4864.883 kb on - strand, within Pf6N2E2_4180 at 4864.883 kb on - strand, within Pf6N2E2_4180 at 4864.934 kb on - strand, within Pf6N2E2_4180 at 4865.210 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.221 kb on + strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180 at 4865.222 kb on - strand, within Pf6N2E2_4180
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 4,862,738 - -0.2 4,862,882 + -0.9 4,862,882 + +0.8 4,862,883 - -1.2 4,862,883 - -0.3 4,863,023 - Pf6N2E2_4178 0.20 -0.6 4,863,092 + Pf6N2E2_4178 0.30 +0.6 4,863,093 - Pf6N2E2_4178 0.30 -1.2 4,863,093 - Pf6N2E2_4178 0.30 -2.8 4,863,093 - Pf6N2E2_4178 0.30 -1.0 4,863,093 - Pf6N2E2_4178 0.30 +0.7 4,863,093 - Pf6N2E2_4178 0.30 +1.0 4,863,149 + Pf6N2E2_4178 0.39 +0.0 4,863,149 + Pf6N2E2_4178 0.39 -3.7 4,863,150 - Pf6N2E2_4178 0.39 +0.2 4,863,194 + Pf6N2E2_4178 0.45 +0.1 4,863,243 + Pf6N2E2_4178 0.52 +0.7 4,863,243 + Pf6N2E2_4178 0.52 +0.1 4,863,244 - Pf6N2E2_4178 0.52 -0.7 4,863,244 - Pf6N2E2_4178 0.52 -1.1 4,863,244 - Pf6N2E2_4178 0.52 +0.5 4,863,244 - Pf6N2E2_4178 0.52 -0.0 4,863,244 - Pf6N2E2_4178 0.52 +0.2 4,863,244 - Pf6N2E2_4178 0.52 -0.3 4,863,244 - Pf6N2E2_4178 0.52 -0.2 4,863,244 - Pf6N2E2_4178 0.52 +1.0 4,863,311 + Pf6N2E2_4178 0.62 +0.2 4,863,311 + Pf6N2E2_4178 0.62 -0.9 4,863,383 + Pf6N2E2_4178 0.72 +1.3 4,863,384 - Pf6N2E2_4178 0.72 -0.5 4,863,384 - Pf6N2E2_4178 0.72 -0.5 4,863,384 - Pf6N2E2_4178 0.72 -0.4 4,863,384 - Pf6N2E2_4178 0.72 +0.4 4,863,522 - +0.6 4,863,522 - -1.9 4,863,534 + -1.5 4,863,535 - -1.1 4,863,535 - -0.0 4,863,677 + +1.6 4,863,702 - +0.8 4,863,850 + Pf6N2E2_4179 0.22 -1.5 4,863,850 + Pf6N2E2_4179 0.22 -2.7 4,863,850 + Pf6N2E2_4179 0.22 -0.2 4,863,850 + Pf6N2E2_4179 0.22 -0.1 4,863,850 + Pf6N2E2_4179 0.22 -0.9 4,863,850 + Pf6N2E2_4179 0.22 -1.8 4,863,850 + Pf6N2E2_4179 0.22 +0.3 4,863,851 - Pf6N2E2_4179 0.22 +1.8 4,863,851 - Pf6N2E2_4179 0.22 +0.8 4,863,853 + Pf6N2E2_4179 0.22 -0.9 4,863,936 - Pf6N2E2_4179 0.37 -0.5 4,863,976 + Pf6N2E2_4179 0.44 -0.8 4,863,976 + Pf6N2E2_4179 0.44 -0.0 4,863,977 - Pf6N2E2_4179 0.44 +0.6 4,863,977 - Pf6N2E2_4179 0.44 +0.3 4,864,048 + Pf6N2E2_4179 0.57 +0.1 4,864,048 + Pf6N2E2_4179 0.57 -0.7 4,864,048 + Pf6N2E2_4179 0.57 -2.1 4,864,070 + Pf6N2E2_4179 0.61 -1.7 4,864,071 - Pf6N2E2_4179 0.61 +0.6 4,864,071 - Pf6N2E2_4179 0.61 +0.8 4,864,071 - Pf6N2E2_4179 0.61 -0.1 4,864,124 + Pf6N2E2_4179 0.70 -2.2 4,864,147 - Pf6N2E2_4179 0.74 -1.1 4,864,147 - Pf6N2E2_4179 0.74 -1.7 4,864,183 + Pf6N2E2_4179 0.81 -0.7 4,864,183 + Pf6N2E2_4179 0.81 +2.1 4,864,183 + Pf6N2E2_4179 0.81 -0.3 4,864,184 - Pf6N2E2_4179 0.81 -1.4 4,864,184 - Pf6N2E2_4179 0.81 +0.1 4,864,185 + Pf6N2E2_4179 0.81 -3.0 4,864,208 + Pf6N2E2_4179 0.85 -0.3 4,864,209 - Pf6N2E2_4179 0.85 -0.5 4,864,209 - Pf6N2E2_4179 0.85 -1.2 4,864,209 - Pf6N2E2_4179 0.85 +1.4 4,864,253 + -1.7 4,864,253 + -1.1 4,864,261 + +0.8 4,864,396 + +2.8 4,864,409 + +1.5 4,864,410 - +0.9 4,864,427 + +0.6 4,864,428 - -1.9 4,864,502 + -0.2 4,864,502 + +0.5 4,864,502 + -0.9 4,864,503 - +1.2 4,864,503 - +0.6 4,864,536 + +0.9 4,864,536 + -0.6 4,864,536 + -0.2 4,864,536 + +1.8 4,864,536 + +0.8 4,864,606 + -2.4 4,864,607 - +1.0 4,864,607 - -0.2 4,864,607 - -0.0 4,864,607 - +0.1 4,864,610 + -1.5 4,864,610 + +2.8 4,864,611 - -0.2 4,864,611 - +1.3 4,864,611 - -1.2 4,864,611 - +1.8 4,864,611 - -0.3 4,864,611 - +0.5 4,864,781 + Pf6N2E2_4180 0.25 -0.9 4,864,782 - Pf6N2E2_4180 0.25 -1.4 4,864,801 - Pf6N2E2_4180 0.27 +0.5 4,864,810 + Pf6N2E2_4180 0.28 -0.7 4,864,810 + Pf6N2E2_4180 0.28 +0.6 4,864,810 + Pf6N2E2_4180 0.28 +1.3 4,864,882 + Pf6N2E2_4180 0.34 -3.5 4,864,882 + Pf6N2E2_4180 0.34 +0.4 4,864,883 - Pf6N2E2_4180 0.34 +1.2 4,864,883 - Pf6N2E2_4180 0.34 +0.2 4,864,934 - Pf6N2E2_4180 0.39 +1.1 4,865,210 + Pf6N2E2_4180 0.63 -0.1 4,865,221 + Pf6N2E2_4180 0.64 +1.8 4,865,221 + Pf6N2E2_4180 0.64 -0.7 4,865,221 + Pf6N2E2_4180 0.64 +0.0 4,865,221 + Pf6N2E2_4180 0.64 -1.8 4,865,221 + Pf6N2E2_4180 0.64 +0.4 4,865,221 + Pf6N2E2_4180 0.64 +0.9 4,865,221 + Pf6N2E2_4180 0.64 -0.6 4,865,221 + Pf6N2E2_4180 0.64 +0.5 4,865,221 + Pf6N2E2_4180 0.64 +2.5 4,865,222 - Pf6N2E2_4180 0.64 +2.6 4,865,222 - Pf6N2E2_4180 0.64 +0.8 4,865,222 - Pf6N2E2_4180 0.64 -1.2 4,865,222 - Pf6N2E2_4180 0.64 +0.8 4,865,222 - Pf6N2E2_4180 0.64 +0.8 4,865,222 - Pf6N2E2_4180 0.64 +0.5 4,865,222 - Pf6N2E2_4180 0.64 -0.3
Or see this region's nucleotide sequence