Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3151 and Pf6N2E2_3152 are separated by 12 nucleotides Pf6N2E2_3152 and Pf6N2E2_3153 are separated by 430 nucleotides
Pf6N2E2_3151 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 3,643,453 to 3,644,265
_3151
Pf6N2E2_3152 - Magnesium and cobalt efflux protein CorC, at 3,644,278 to 3,645,519
_3152
Pf6N2E2_3153 - Permeases of the major facilitator superfamily, at 3,645,950 to 3,647,263
_3153
Position (kb)
3644
3645
3646 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 3643.346 kb on - strand at 3643.365 kb on + strand at 3643.365 kb on + strand at 3643.366 kb on - strand at 3643.366 kb on - strand at 3643.366 kb on - strand at 3643.366 kb on - strand at 3643.412 kb on - strand at 3643.412 kb on - strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.452 kb on + strand at 3643.504 kb on + strand at 3643.505 kb on - strand at 3643.505 kb on - strand at 3643.522 kb on + strand at 3643.618 kb on + strand, within Pf6N2E2_3151 at 3643.618 kb on + strand, within Pf6N2E2_3151 at 3643.618 kb on + strand, within Pf6N2E2_3151 at 3643.620 kb on + strand, within Pf6N2E2_3151 at 3643.803 kb on + strand, within Pf6N2E2_3151 at 3643.804 kb on - strand, within Pf6N2E2_3151 at 3643.867 kb on + strand, within Pf6N2E2_3151 at 3644.222 kb on - strand at 3644.248 kb on + strand at 3644.248 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.283 kb on + strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.284 kb on - strand at 3644.581 kb on + strand, within Pf6N2E2_3152 at 3644.581 kb on + strand, within Pf6N2E2_3152 at 3644.582 kb on - strand, within Pf6N2E2_3152 at 3644.651 kb on + strand, within Pf6N2E2_3152 at 3644.652 kb on - strand, within Pf6N2E2_3152 at 3644.668 kb on + strand, within Pf6N2E2_3152 at 3644.691 kb on + strand, within Pf6N2E2_3152 at 3644.692 kb on - strand, within Pf6N2E2_3152 at 3644.692 kb on - strand, within Pf6N2E2_3152 at 3645.079 kb on + strand, within Pf6N2E2_3152 at 3645.079 kb on + strand, within Pf6N2E2_3152 at 3645.080 kb on - strand, within Pf6N2E2_3152 at 3645.080 kb on - strand, within Pf6N2E2_3152 at 3645.080 kb on - strand, within Pf6N2E2_3152 at 3645.080 kb on - strand, within Pf6N2E2_3152 at 3645.080 kb on - strand, within Pf6N2E2_3152 at 3645.167 kb on - strand, within Pf6N2E2_3152 at 3645.492 kb on + strand at 3645.492 kb on + strand at 3645.493 kb on - strand at 3645.493 kb on - strand at 3645.606 kb on - strand at 3645.732 kb on - strand at 3645.732 kb on - strand at 3645.732 kb on - strand at 3645.732 kb on - strand at 3645.747 kb on + strand at 3645.748 kb on - strand at 3645.748 kb on - strand at 3645.749 kb on + strand at 3645.750 kb on - strand at 3645.750 kb on - strand at 3645.750 kb on - strand at 3645.750 kb on - strand at 3645.750 kb on - strand at 3645.905 kb on + strand at 3645.905 kb on + strand at 3645.906 kb on - strand at 3645.908 kb on + strand at 3645.908 kb on + strand at 3645.908 kb on + strand at 3645.908 kb on + strand at 3645.908 kb on + strand at 3645.909 kb on - strand at 3645.909 kb on - strand at 3645.909 kb on - strand at 3645.909 kb on - strand at 3645.961 kb on + strand at 3645.961 kb on + strand at 3645.962 kb on - strand at 3646.049 kb on + strand at 3646.050 kb on - strand at 3646.058 kb on + strand at 3646.059 kb on - strand at 3646.059 kb on - strand at 3646.059 kb on - strand at 3646.059 kb on - strand at 3646.064 kb on + strand at 3646.064 kb on + strand at 3646.064 kb on + strand at 3646.064 kb on + strand at 3646.064 kb on + strand at 3646.065 kb on - strand at 3646.065 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,643,346 - +0.9 3,643,365 + +1.2 3,643,365 + -0.3 3,643,366 - +2.5 3,643,366 - +0.8 3,643,366 - +1.9 3,643,366 - -2.2 3,643,412 - +5.2 3,643,412 - +5.1 3,643,452 + +4.0 3,643,452 + +4.2 3,643,452 + +4.0 3,643,452 + +3.6 3,643,452 + +4.1 3,643,452 + +3.4 3,643,452 + +4.0 3,643,504 + +3.8 3,643,505 - +5.9 3,643,505 - +4.6 3,643,522 + +4.2 3,643,618 + Pf6N2E2_3151 0.20 +4.0 3,643,618 + Pf6N2E2_3151 0.20 +3.5 3,643,618 + Pf6N2E2_3151 0.20 +5.9 3,643,620 + Pf6N2E2_3151 0.21 +6.0 3,643,803 + Pf6N2E2_3151 0.43 +4.6 3,643,804 - Pf6N2E2_3151 0.43 +5.7 3,643,867 + Pf6N2E2_3151 0.51 +3.8 3,644,222 - +4.9 3,644,248 + +1.1 3,644,248 + -0.3 3,644,283 + +4.7 3,644,283 + +5.5 3,644,283 + +6.1 3,644,283 + +5.0 3,644,283 + +5.9 3,644,283 + +5.8 3,644,283 + +5.7 3,644,284 - +3.8 3,644,284 - +3.9 3,644,284 - +5.9 3,644,284 - +5.8 3,644,284 - +5.0 3,644,284 - +3.0 3,644,284 - +5.8 3,644,284 - -2.1 3,644,581 + Pf6N2E2_3152 0.24 +3.9 3,644,581 + Pf6N2E2_3152 0.24 -0.9 3,644,582 - Pf6N2E2_3152 0.24 +5.0 3,644,651 + Pf6N2E2_3152 0.30 +5.3 3,644,652 - Pf6N2E2_3152 0.30 +5.6 3,644,668 + Pf6N2E2_3152 0.31 +3.8 3,644,691 + Pf6N2E2_3152 0.33 +3.0 3,644,692 - Pf6N2E2_3152 0.33 +5.9 3,644,692 - Pf6N2E2_3152 0.33 +4.6 3,645,079 + Pf6N2E2_3152 0.64 +5.7 3,645,079 + Pf6N2E2_3152 0.64 +5.9 3,645,080 - Pf6N2E2_3152 0.65 +5.6 3,645,080 - Pf6N2E2_3152 0.65 +3.6 3,645,080 - Pf6N2E2_3152 0.65 +6.3 3,645,080 - Pf6N2E2_3152 0.65 +5.7 3,645,080 - Pf6N2E2_3152 0.65 +4.8 3,645,167 - Pf6N2E2_3152 0.72 +6.0 3,645,492 + +5.0 3,645,492 + +3.5 3,645,493 - +0.2 3,645,493 - +5.7 3,645,606 - +2.1 3,645,732 - +1.2 3,645,732 - -2.7 3,645,732 - -0.4 3,645,732 - +1.4 3,645,747 + -2.4 3,645,748 - +1.2 3,645,748 - -0.8 3,645,749 + -2.2 3,645,750 - +1.1 3,645,750 - +2.0 3,645,750 - +0.7 3,645,750 - +1.2 3,645,750 - +0.7 3,645,905 + +1.2 3,645,905 + +0.4 3,645,906 - +0.8 3,645,908 + +0.2 3,645,908 + -0.4 3,645,908 + +0.2 3,645,908 + -1.9 3,645,908 + +0.2 3,645,909 - +2.0 3,645,909 - +0.6 3,645,909 - +0.9 3,645,909 - +1.6 3,645,961 + +1.4 3,645,961 + +0.5 3,645,962 - +1.5 3,646,049 + +1.4 3,646,050 - +0.2 3,646,058 + +1.0 3,646,059 - +0.5 3,646,059 - +2.2 3,646,059 - +1.5 3,646,059 - -1.5 3,646,064 + +0.2 3,646,064 + +0.6 3,646,064 + +1.8 3,646,064 + -0.5 3,646,064 + +0.1 3,646,065 - +1.7 3,646,065 - +1.6
Or see this region's nucleotide sequence