Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3078 and Pf6N2E2_3079 are separated by 174 nucleotides Pf6N2E2_3079 and Pf6N2E2_3080 overlap by 4 nucleotides Pf6N2E2_3080 and Pf6N2E2_3081 are separated by 126 nucleotides
Pf6N2E2_3078 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,566,203 to 3,567,237
_3078
Pf6N2E2_3079 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,567,412 to 3,568,617
_3079
Pf6N2E2_3080 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,568,614 to 3,569,021
_3080
Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966
_3081
Position (kb)
3567
3568
3569 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.235 kb on + strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.236 kb on - strand at 3567.389 kb on + strand at 3567.390 kb on - strand at 3567.481 kb on + strand at 3567.482 kb on - strand at 3567.482 kb on - strand at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.541 kb on + strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.542 kb on - strand, within Pf6N2E2_3079 at 3567.544 kb on + strand, within Pf6N2E2_3079 at 3567.544 kb on + strand, within Pf6N2E2_3079 at 3567.545 kb on - strand, within Pf6N2E2_3079 at 3567.728 kb on + strand, within Pf6N2E2_3079 at 3567.729 kb on - strand, within Pf6N2E2_3079 at 3567.729 kb on - strand, within Pf6N2E2_3079 at 3567.921 kb on - strand, within Pf6N2E2_3079 at 3568.060 kb on + strand, within Pf6N2E2_3079 at 3568.061 kb on - strand, within Pf6N2E2_3079 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.334 kb on - strand, within Pf6N2E2_3079 at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.968 kb on - strand, within Pf6N2E2_3080 at 3569.201 kb on + strand at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,567,235 + -1.6 3,567,235 + +1.0 3,567,235 + -0.7 3,567,235 + -0.5 3,567,235 + -0.1 3,567,236 - -2.9 3,567,236 - -3.0 3,567,236 - -2.9 3,567,236 - -0.8 3,567,236 - -1.8 3,567,236 - -2.4 3,567,389 + -1.4 3,567,390 - -1.1 3,567,481 + -1.8 3,567,482 - -0.7 3,567,482 - -4.9 3,567,541 + Pf6N2E2_3079 0.11 -0.8 3,567,541 + Pf6N2E2_3079 0.11 +1.2 3,567,541 + Pf6N2E2_3079 0.11 +0.4 3,567,541 + Pf6N2E2_3079 0.11 -2.5 3,567,542 - Pf6N2E2_3079 0.11 -1.6 3,567,542 - Pf6N2E2_3079 0.11 -0.4 3,567,542 - Pf6N2E2_3079 0.11 +1.2 3,567,542 - Pf6N2E2_3079 0.11 -1.6 3,567,544 + Pf6N2E2_3079 0.11 -0.8 3,567,544 + Pf6N2E2_3079 0.11 +0.2 3,567,545 - Pf6N2E2_3079 0.11 +1.2 3,567,728 + Pf6N2E2_3079 0.26 -2.3 3,567,729 - Pf6N2E2_3079 0.26 -3.1 3,567,729 - Pf6N2E2_3079 0.26 -2.6 3,567,921 - Pf6N2E2_3079 0.42 -1.8 3,568,060 + Pf6N2E2_3079 0.54 -1.4 3,568,061 - Pf6N2E2_3079 0.54 -1.6 3,568,277 - Pf6N2E2_3079 0.72 -2.7 3,568,277 - Pf6N2E2_3079 0.72 -0.3 3,568,333 + Pf6N2E2_3079 0.76 -2.9 3,568,333 + Pf6N2E2_3079 0.76 -2.2 3,568,333 + Pf6N2E2_3079 0.76 -2.0 3,568,334 - Pf6N2E2_3079 0.76 -1.4 3,568,549 + -0.7 3,568,549 + -1.8 3,568,549 + -4.1 3,568,549 + -2.0 3,568,968 - Pf6N2E2_3080 0.87 +0.2 3,569,201 + -2.6 3,569,380 + Pf6N2E2_3081 0.28 -4.1 3,569,380 + Pf6N2E2_3081 0.28 -3.5 3,569,381 - Pf6N2E2_3081 0.28 -1.6 3,569,381 - Pf6N2E2_3081 0.28 -2.1 3,569,433 + Pf6N2E2_3081 0.35 -0.4 3,569,433 + Pf6N2E2_3081 0.35 -0.4 3,569,433 + Pf6N2E2_3081 0.35 -1.8 3,569,434 - Pf6N2E2_3081 0.35 -0.6 3,569,434 - Pf6N2E2_3081 0.35 -1.8
Or see this region's nucleotide sequence