Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2746 and Pf6N2E2_2747 are separated by 17 nucleotides Pf6N2E2_2747 and Pf6N2E2_2748 are separated by 315 nucleotides Pf6N2E2_2748 and Pf6N2E2_2749 are separated by 10 nucleotides
Pf6N2E2_2746 - Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor, at 3,238,554 to 3,239,669
_2746
Pf6N2E2_2747 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 3,239,687 to 3,240,565
_2747
Pf6N2E2_2748 - Glycine cleavage system transcriptional antiactivator GcvR, at 3,240,881 to 3,241,441
_2748
Pf6N2E2_2749 - Thiol peroxidase, Bcp-type (EC 1.11.1.15), at 3,241,452 to 3,241,925
_2749
Position (kb)
3239
3240
3241 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.790 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3238.793 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.114 kb on - strand, within Pf6N2E2_2746 at 3239.390 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.441 kb on - strand, within Pf6N2E2_2746 at 3239.585 kb on - strand at 3239.631 kb on - strand at 3239.631 kb on - strand at 3239.689 kb on - strand at 3240.633 kb on + strand at 3240.633 kb on + strand at 3240.634 kb on - strand at 3240.634 kb on - strand at 3240.696 kb on + strand at 3240.696 kb on + strand at 3240.696 kb on + strand at 3240.697 kb on - strand at 3240.697 kb on - strand at 3240.697 kb on - strand at 3240.953 kb on - strand, within Pf6N2E2_2748 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.172 kb on + strand, within Pf6N2E2_2748 at 3241.173 kb on - strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.181 kb on + strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.182 kb on - strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.277 kb on + strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.278 kb on - strand, within Pf6N2E2_2748 at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.439 kb on + strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.440 kb on - strand at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.554 kb on + strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.555 kb on - strand, within Pf6N2E2_2749 at 3241.560 kb on + strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749 at 3241.561 kb on - strand, within Pf6N2E2_2749
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,238,790 - Pf6N2E2_2746 0.21 -0.5 3,238,790 - Pf6N2E2_2746 0.21 -1.9 3,238,790 - Pf6N2E2_2746 0.21 -0.4 3,238,790 - Pf6N2E2_2746 0.21 +1.1 3,238,790 - Pf6N2E2_2746 0.21 +0.7 3,238,790 - Pf6N2E2_2746 0.21 -0.4 3,238,793 - Pf6N2E2_2746 0.21 -1.2 3,238,793 - Pf6N2E2_2746 0.21 -0.9 3,238,793 - Pf6N2E2_2746 0.21 +0.1 3,238,793 - Pf6N2E2_2746 0.21 +0.6 3,238,793 - Pf6N2E2_2746 0.21 +0.1 3,238,793 - Pf6N2E2_2746 0.21 -1.2 3,238,793 - Pf6N2E2_2746 0.21 +0.1 3,239,114 - Pf6N2E2_2746 0.50 +1.1 3,239,114 - Pf6N2E2_2746 0.50 +1.1 3,239,114 - Pf6N2E2_2746 0.50 -1.2 3,239,114 - Pf6N2E2_2746 0.50 -2.9 3,239,390 - Pf6N2E2_2746 0.75 -3.4 3,239,441 - Pf6N2E2_2746 0.79 -0.9 3,239,441 - Pf6N2E2_2746 0.79 -0.4 3,239,585 - +0.1 3,239,631 - +1.1 3,239,631 - -0.4 3,239,689 - +1.1 3,240,633 + +1.1 3,240,633 + -1.2 3,240,634 - -0.6 3,240,634 - +1.8 3,240,696 + +0.7 3,240,696 + +0.1 3,240,696 + +1.7 3,240,697 - +1.7 3,240,697 - +2.1 3,240,697 - +1.1 3,240,953 - Pf6N2E2_2748 0.13 +1.1 3,241,172 + Pf6N2E2_2748 0.52 +0.6 3,241,172 + Pf6N2E2_2748 0.52 +1.1 3,241,173 - Pf6N2E2_2748 0.52 -0.5 3,241,181 + Pf6N2E2_2748 0.53 +1.0 3,241,181 + Pf6N2E2_2748 0.53 +0.0 3,241,181 + Pf6N2E2_2748 0.53 +0.6 3,241,181 + Pf6N2E2_2748 0.53 +0.8 3,241,182 - Pf6N2E2_2748 0.54 -1.7 3,241,182 - Pf6N2E2_2748 0.54 -0.4 3,241,182 - Pf6N2E2_2748 0.54 +1.6 3,241,182 - Pf6N2E2_2748 0.54 +2.2 3,241,182 - Pf6N2E2_2748 0.54 -0.0 3,241,182 - Pf6N2E2_2748 0.54 -3.4 3,241,277 + Pf6N2E2_2748 0.71 +2.4 3,241,277 + Pf6N2E2_2748 0.71 +0.8 3,241,277 + Pf6N2E2_2748 0.71 +0.6 3,241,277 + Pf6N2E2_2748 0.71 +0.6 3,241,277 + Pf6N2E2_2748 0.71 +1.5 3,241,277 + Pf6N2E2_2748 0.71 +0.1 3,241,278 - Pf6N2E2_2748 0.71 -0.1 3,241,278 - Pf6N2E2_2748 0.71 +1.1 3,241,278 - Pf6N2E2_2748 0.71 -0.9 3,241,278 - Pf6N2E2_2748 0.71 +1.1 3,241,278 - Pf6N2E2_2748 0.71 +1.4 3,241,278 - Pf6N2E2_2748 0.71 +1.1 3,241,439 + +0.7 3,241,439 + -0.3 3,241,439 + +2.1 3,241,439 + +0.7 3,241,439 + +0.6 3,241,440 - +1.1 3,241,440 - +1.0 3,241,440 - -1.9 3,241,440 - -1.4 3,241,440 - +2.9 3,241,554 + Pf6N2E2_2749 0.22 -1.2 3,241,554 + Pf6N2E2_2749 0.22 -0.7 3,241,554 + Pf6N2E2_2749 0.22 -0.4 3,241,554 + Pf6N2E2_2749 0.22 -0.9 3,241,554 + Pf6N2E2_2749 0.22 -1.3 3,241,555 - Pf6N2E2_2749 0.22 -2.1 3,241,555 - Pf6N2E2_2749 0.22 +1.1 3,241,555 - Pf6N2E2_2749 0.22 -1.3 3,241,560 + Pf6N2E2_2749 0.23 -0.9 3,241,561 - Pf6N2E2_2749 0.23 +1.1 3,241,561 - Pf6N2E2_2749 0.23 -2.7 3,241,561 - Pf6N2E2_2749 0.23 +0.8
Or see this region's nucleotide sequence