Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2666 and Pf6N2E2_2667 are separated by 62 nucleotides Pf6N2E2_2667 and Pf6N2E2_2668 are separated by 298 nucleotides Pf6N2E2_2668 and Pf6N2E2_2669 are separated by 653 nucleotides
Pf6N2E2_2666 - hypothetical protein, at 3,139,226 to 3,140,092
_2666
Pf6N2E2_2667 - hypothetical protein, at 3,140,155 to 3,140,547
_2667
Pf6N2E2_2668 - FIG00960825: hypothetical protein, at 3,140,846 to 3,141,577
_2668
Pf6N2E2_2669 - hypothetical protein, at 3,142,231 to 3,142,533
_2669
Position (kb)
3140
3141
3142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3140.090 kb on + strand at 3140.090 kb on + strand at 3140.090 kb on + strand at 3140.091 kb on - strand at 3140.350 kb on - strand, within Pf6N2E2_2667 at 3140.351 kb on + strand, within Pf6N2E2_2667 at 3140.352 kb on - strand, within Pf6N2E2_2667 at 3140.352 kb on - strand, within Pf6N2E2_2667 at 3140.352 kb on - strand, within Pf6N2E2_2667 at 3140.352 kb on - strand, within Pf6N2E2_2667 at 3140.378 kb on + strand, within Pf6N2E2_2667 at 3140.378 kb on + strand, within Pf6N2E2_2667 at 3140.451 kb on - strand, within Pf6N2E2_2667 at 3140.451 kb on - strand, within Pf6N2E2_2667 at 3140.451 kb on - strand, within Pf6N2E2_2667 at 3140.462 kb on + strand, within Pf6N2E2_2667 at 3140.462 kb on + strand, within Pf6N2E2_2667 at 3140.476 kb on - strand, within Pf6N2E2_2667 at 3140.481 kb on + strand, within Pf6N2E2_2667 at 3140.481 kb on + strand, within Pf6N2E2_2667 at 3140.482 kb on - strand, within Pf6N2E2_2667 at 3140.535 kb on + strand at 3140.536 kb on - strand at 3140.536 kb on - strand at 3140.536 kb on - strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.537 kb on + strand at 3140.538 kb on - strand at 3140.538 kb on - strand at 3140.538 kb on - strand at 3140.538 kb on - strand at 3140.538 kb on - strand at 3140.538 kb on - strand at 3140.578 kb on + strand at 3140.578 kb on + strand at 3140.579 kb on - strand at 3140.579 kb on - strand at 3140.582 kb on + strand at 3140.582 kb on + strand at 3140.582 kb on + strand at 3140.582 kb on + strand at 3140.583 kb on - strand at 3140.583 kb on - strand at 3140.583 kb on - strand at 3140.583 kb on - strand at 3140.583 kb on - strand at 3140.641 kb on + strand at 3140.641 kb on + strand at 3140.641 kb on + strand at 3140.642 kb on - strand at 3140.642 kb on - strand at 3140.642 kb on - strand at 3140.642 kb on - strand at 3140.679 kb on + strand at 3140.680 kb on - strand at 3140.726 kb on - strand at 3140.726 kb on - strand at 3140.734 kb on - strand at 3140.740 kb on + strand at 3140.744 kb on + strand at 3140.744 kb on + strand at 3140.745 kb on - strand at 3140.745 kb on - strand at 3140.745 kb on - strand at 3140.799 kb on + strand at 3140.800 kb on - strand at 3141.068 kb on + strand, within Pf6N2E2_2668 at 3141.069 kb on - strand, within Pf6N2E2_2668 at 3141.069 kb on - strand, within Pf6N2E2_2668 at 3141.278 kb on + strand, within Pf6N2E2_2668 at 3141.279 kb on - strand, within Pf6N2E2_2668 at 3141.279 kb on - strand, within Pf6N2E2_2668 at 3141.279 kb on - strand, within Pf6N2E2_2668 at 3141.332 kb on + strand, within Pf6N2E2_2668 at 3141.332 kb on + strand, within Pf6N2E2_2668 at 3141.333 kb on - strand, within Pf6N2E2_2668 at 3141.333 kb on - strand, within Pf6N2E2_2668 at 3141.339 kb on - strand, within Pf6N2E2_2668 at 3141.389 kb on + strand, within Pf6N2E2_2668 at 3141.422 kb on + strand, within Pf6N2E2_2668 at 3141.423 kb on - strand, within Pf6N2E2_2668 at 3141.423 kb on - strand, within Pf6N2E2_2668 at 3141.423 kb on - strand, within Pf6N2E2_2668 at 3141.423 kb on - strand, within Pf6N2E2_2668 at 3141.423 kb on - strand, within Pf6N2E2_2668 at 3141.432 kb on - strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.464 kb on + strand, within Pf6N2E2_2668 at 3141.465 kb on - strand, within Pf6N2E2_2668 at 3141.465 kb on - strand, within Pf6N2E2_2668 at 3141.470 kb on + strand, within Pf6N2E2_2668 at 3141.470 kb on + strand, within Pf6N2E2_2668 at 3141.470 kb on + strand, within Pf6N2E2_2668 at 3141.470 kb on + strand, within Pf6N2E2_2668 at 3141.470 kb on + strand, within Pf6N2E2_2668 at 3141.471 kb on - strand, within Pf6N2E2_2668 at 3141.535 kb on + strand at 3141.535 kb on + strand at 3141.539 kb on + strand at 3141.540 kb on - strand at 3141.540 kb on - strand at 3141.710 kb on + strand at 3141.759 kb on + strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.760 kb on - strand at 3141.761 kb on + strand at 3141.761 kb on + strand at 3141.762 kb on - strand at 3141.762 kb on - strand at 3141.762 kb on - strand at 3141.762 kb on - strand at 3141.841 kb on - strand at 3141.841 kb on - strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.850 kb on + strand at 3141.851 kb on - strand at 3141.851 kb on - strand at 3141.851 kb on - strand at 3141.851 kb on - strand at 3141.877 kb on - strand at 3142.037 kb on + strand at 3142.044 kb on - strand at 3142.152 kb on + strand at 3142.152 kb on + strand at 3142.152 kb on + strand at 3142.152 kb on + strand at 3142.153 kb on - strand at 3142.153 kb on - strand at 3142.164 kb on + strand at 3142.186 kb on + strand at 3142.186 kb on + strand at 3142.186 kb on + strand at 3142.187 kb on - strand at 3142.187 kb on - strand at 3142.187 kb on - strand at 3142.187 kb on - strand at 3142.190 kb on + strand at 3142.190 kb on + strand at 3142.218 kb on + strand at 3142.224 kb on - strand at 3142.302 kb on + strand, within Pf6N2E2_2669 at 3142.303 kb on - strand, within Pf6N2E2_2669 at 3142.303 kb on - strand, within Pf6N2E2_2669 at 3142.303 kb on - strand, within Pf6N2E2_2669 at 3142.303 kb on - strand, within Pf6N2E2_2669 at 3142.329 kb on - strand, within Pf6N2E2_2669 at 3142.329 kb on - strand, within Pf6N2E2_2669 at 3142.337 kb on + strand, within Pf6N2E2_2669 at 3142.337 kb on + strand, within Pf6N2E2_2669 at 3142.338 kb on - strand, within Pf6N2E2_2669 at 3142.368 kb on + strand, within Pf6N2E2_2669 at 3142.368 kb on + strand, within Pf6N2E2_2669 at 3142.368 kb on + strand, within Pf6N2E2_2669 at 3142.369 kb on - strand, within Pf6N2E2_2669 at 3142.379 kb on + strand, within Pf6N2E2_2669 at 3142.379 kb on + strand, within Pf6N2E2_2669 at 3142.380 kb on - strand, within Pf6N2E2_2669 at 3142.403 kb on - strand, within Pf6N2E2_2669 at 3142.411 kb on + strand, within Pf6N2E2_2669 at 3142.411 kb on + strand, within Pf6N2E2_2669 at 3142.411 kb on + strand, within Pf6N2E2_2669 at 3142.412 kb on - strand, within Pf6N2E2_2669 at 3142.412 kb on - strand, within Pf6N2E2_2669 at 3142.418 kb on - strand, within Pf6N2E2_2669 at 3142.502 kb on + strand, within Pf6N2E2_2669 at 3142.502 kb on + strand, within Pf6N2E2_2669 at 3142.503 kb on - strand at 3142.503 kb on - strand at 3142.503 kb on - strand at 3142.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,140,090 + -2.6 3,140,090 + -0.8 3,140,090 + -1.1 3,140,091 - -0.4 3,140,350 - Pf6N2E2_2667 0.50 -1.8 3,140,351 + Pf6N2E2_2667 0.50 +0.6 3,140,352 - Pf6N2E2_2667 0.50 -0.6 3,140,352 - Pf6N2E2_2667 0.50 -1.5 3,140,352 - Pf6N2E2_2667 0.50 +0.4 3,140,352 - Pf6N2E2_2667 0.50 -1.9 3,140,378 + Pf6N2E2_2667 0.57 +0.6 3,140,378 + Pf6N2E2_2667 0.57 -1.2 3,140,451 - Pf6N2E2_2667 0.75 +1.1 3,140,451 - Pf6N2E2_2667 0.75 +0.7 3,140,451 - Pf6N2E2_2667 0.75 +1.8 3,140,462 + Pf6N2E2_2667 0.78 +0.4 3,140,462 + Pf6N2E2_2667 0.78 +1.1 3,140,476 - Pf6N2E2_2667 0.82 +0.3 3,140,481 + Pf6N2E2_2667 0.83 +0.4 3,140,481 + Pf6N2E2_2667 0.83 +3.8 3,140,482 - Pf6N2E2_2667 0.83 -1.5 3,140,535 + +1.2 3,140,536 - -0.9 3,140,536 - -0.5 3,140,536 - -0.2 3,140,537 + +1.1 3,140,537 + +0.1 3,140,537 + +1.0 3,140,537 + -0.4 3,140,537 + +0.2 3,140,537 + +0.1 3,140,537 + +0.9 3,140,538 - -0.5 3,140,538 - -1.5 3,140,538 - +1.1 3,140,538 - -0.3 3,140,538 - +0.5 3,140,538 - +1.1 3,140,578 + -0.2 3,140,578 + -1.5 3,140,579 - -0.3 3,140,579 - +0.3 3,140,582 + -0.1 3,140,582 + +0.7 3,140,582 + +0.0 3,140,582 + +0.1 3,140,583 - +1.5 3,140,583 - -1.7 3,140,583 - -0.6 3,140,583 - -0.5 3,140,583 - +2.4 3,140,641 + +0.1 3,140,641 + -0.1 3,140,641 + +1.1 3,140,642 - -0.5 3,140,642 - -1.2 3,140,642 - -0.6 3,140,642 - -0.4 3,140,679 + +1.1 3,140,680 - -0.9 3,140,726 - +0.0 3,140,726 - +1.5 3,140,734 - +0.1 3,140,740 + +1.3 3,140,744 + +0.4 3,140,744 + -2.8 3,140,745 - +1.1 3,140,745 - +0.0 3,140,745 - -0.5 3,140,799 + +1.4 3,140,800 - -2.2 3,141,068 + Pf6N2E2_2668 0.30 -0.5 3,141,069 - Pf6N2E2_2668 0.30 -0.6 3,141,069 - Pf6N2E2_2668 0.30 +1.1 3,141,278 + Pf6N2E2_2668 0.59 -1.3 3,141,279 - Pf6N2E2_2668 0.59 -1.9 3,141,279 - Pf6N2E2_2668 0.59 -1.8 3,141,279 - Pf6N2E2_2668 0.59 -3.5 3,141,332 + Pf6N2E2_2668 0.66 +0.6 3,141,332 + Pf6N2E2_2668 0.66 -1.9 3,141,333 - Pf6N2E2_2668 0.67 -0.5 3,141,333 - Pf6N2E2_2668 0.67 -0.2 3,141,339 - Pf6N2E2_2668 0.67 -2.2 3,141,389 + Pf6N2E2_2668 0.74 -0.9 3,141,422 + Pf6N2E2_2668 0.79 -1.0 3,141,423 - Pf6N2E2_2668 0.79 +0.6 3,141,423 - Pf6N2E2_2668 0.79 -0.6 3,141,423 - Pf6N2E2_2668 0.79 -3.6 3,141,423 - Pf6N2E2_2668 0.79 +0.1 3,141,423 - Pf6N2E2_2668 0.79 +1.1 3,141,432 - Pf6N2E2_2668 0.80 -1.4 3,141,464 + Pf6N2E2_2668 0.84 +2.0 3,141,464 + Pf6N2E2_2668 0.84 +0.8 3,141,464 + Pf6N2E2_2668 0.84 +1.8 3,141,464 + Pf6N2E2_2668 0.84 -0.9 3,141,464 + Pf6N2E2_2668 0.84 +0.8 3,141,464 + Pf6N2E2_2668 0.84 +1.6 3,141,464 + Pf6N2E2_2668 0.84 +1.2 3,141,464 + Pf6N2E2_2668 0.84 -0.4 3,141,465 - Pf6N2E2_2668 0.85 -1.9 3,141,465 - Pf6N2E2_2668 0.85 -1.0 3,141,470 + Pf6N2E2_2668 0.85 +0.9 3,141,470 + Pf6N2E2_2668 0.85 -1.9 3,141,470 + Pf6N2E2_2668 0.85 +0.1 3,141,470 + Pf6N2E2_2668 0.85 -1.1 3,141,470 + Pf6N2E2_2668 0.85 -1.8 3,141,471 - Pf6N2E2_2668 0.85 -1.2 3,141,535 + -1.6 3,141,535 + +1.1 3,141,539 + -0.5 3,141,540 - -1.9 3,141,540 - -2.6 3,141,710 + +0.0 3,141,759 + +0.4 3,141,760 - +0.1 3,141,760 - -3.4 3,141,760 - +0.7 3,141,760 - -0.8 3,141,760 - -0.9 3,141,760 - -0.4 3,141,760 - +0.4 3,141,760 - -0.4 3,141,760 - -0.3 3,141,761 + -0.1 3,141,761 + +0.6 3,141,762 - +0.3 3,141,762 - +0.0 3,141,762 - +0.1 3,141,762 - +0.1 3,141,841 - +2.9 3,141,841 - +1.2 3,141,850 + +2.9 3,141,850 + +0.8 3,141,850 + -1.0 3,141,850 + -1.2 3,141,850 + -0.2 3,141,850 + -2.2 3,141,850 + -0.6 3,141,850 + -1.2 3,141,850 + -0.9 3,141,850 + +0.8 3,141,850 + -0.7 3,141,850 + +0.4 3,141,851 - +1.6 3,141,851 - +1.3 3,141,851 - +0.6 3,141,851 - -0.9 3,141,877 - -0.3 3,142,037 + +1.0 3,142,044 - +1.0 3,142,152 + -0.4 3,142,152 + -1.5 3,142,152 + -0.5 3,142,152 + -1.9 3,142,153 - -0.7 3,142,153 - -0.9 3,142,164 + -0.5 3,142,186 + +0.7 3,142,186 + +1.5 3,142,186 + +0.7 3,142,187 - +0.4 3,142,187 - +0.5 3,142,187 - -0.9 3,142,187 - -0.5 3,142,190 + +1.3 3,142,190 + +0.2 3,142,218 + -0.2 3,142,224 - -1.9 3,142,302 + Pf6N2E2_2669 0.23 -1.5 3,142,303 - Pf6N2E2_2669 0.24 +0.1 3,142,303 - Pf6N2E2_2669 0.24 -0.5 3,142,303 - Pf6N2E2_2669 0.24 +1.0 3,142,303 - Pf6N2E2_2669 0.24 +2.2 3,142,329 - Pf6N2E2_2669 0.32 -0.7 3,142,329 - Pf6N2E2_2669 0.32 +1.3 3,142,337 + Pf6N2E2_2669 0.35 -1.2 3,142,337 + Pf6N2E2_2669 0.35 -0.4 3,142,338 - Pf6N2E2_2669 0.35 +1.5 3,142,368 + Pf6N2E2_2669 0.45 -1.5 3,142,368 + Pf6N2E2_2669 0.45 +0.5 3,142,368 + Pf6N2E2_2669 0.45 +0.6 3,142,369 - Pf6N2E2_2669 0.46 +0.4 3,142,379 + Pf6N2E2_2669 0.49 -0.5 3,142,379 + Pf6N2E2_2669 0.49 -0.5 3,142,380 - Pf6N2E2_2669 0.49 -0.7 3,142,403 - Pf6N2E2_2669 0.57 +0.5 3,142,411 + Pf6N2E2_2669 0.59 -1.1 3,142,411 + Pf6N2E2_2669 0.59 -0.9 3,142,411 + Pf6N2E2_2669 0.59 +0.8 3,142,412 - Pf6N2E2_2669 0.60 -1.0 3,142,412 - Pf6N2E2_2669 0.60 +0.2 3,142,418 - Pf6N2E2_2669 0.62 +0.8 3,142,502 + Pf6N2E2_2669 0.89 +0.9 3,142,502 + Pf6N2E2_2669 0.89 +0.1 3,142,503 - +1.5 3,142,503 - +1.1 3,142,503 - +0.9 3,142,503 - -0.4
Or see this region's nucleotide sequence