Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2544 and Pf6N2E2_2545 are separated by 181 nucleotides Pf6N2E2_2545 and Pf6N2E2_2546 overlap by 12 nucleotides Pf6N2E2_2546 and Pf6N2E2_2547 are separated by 151 nucleotides Pf6N2E2_2547 and Pf6N2E2_2548 are separated by 12 nucleotides
Pf6N2E2_2544 - Deblocking aminopeptidase (EC 3.4.11.-) @ Cyanophycinase 2 (EC 3.4.15.6), at 3,006,167 to 3,007,357
_2544
Pf6N2E2_2545 - FIG00953242: hypothetical protein, at 3,007,539 to 3,007,766
_2545
Pf6N2E2_2546 - Carbon storage regulator, at 3,007,755 to 3,007,955
_2546
Pf6N2E2_2547 - Protein of unknown function UPF0060, at 3,008,107 to 3,008,484
_2547
Pf6N2E2_2548 - Oxidoreductase, short chain dehydrogenase/reductase family, at 3,008,497 to 3,009,312
_2548
Position (kb)
3008
3009 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.202 kb on + strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.203 kb on - strand, within Pf6N2E2_2544 at 3007.517 kb on + strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.518 kb on - strand at 3007.756 kb on + strand at 3007.757 kb on - strand at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.813 kb on + strand, within Pf6N2E2_2546 at 3007.904 kb on + strand, within Pf6N2E2_2546 at 3007.904 kb on + strand, within Pf6N2E2_2546 at 3007.932 kb on + strand, within Pf6N2E2_2546 at 3007.932 kb on + strand, within Pf6N2E2_2546 at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.947 kb on + strand at 3007.948 kb on - strand at 3007.948 kb on - strand at 3007.948 kb on - strand at 3008.044 kb on - strand at 3008.044 kb on - strand at 3008.104 kb on - strand at 3008.104 kb on - strand at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.238 kb on + strand, within Pf6N2E2_2547 at 3008.239 kb on - strand, within Pf6N2E2_2547 at 3008.239 kb on - strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.240 kb on + strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.241 kb on - strand, within Pf6N2E2_2547 at 3008.253 kb on - strand, within Pf6N2E2_2547 at 3008.261 kb on + strand, within Pf6N2E2_2547 at 3008.276 kb on + strand, within Pf6N2E2_2547 at 3008.276 kb on + strand, within Pf6N2E2_2547 at 3008.277 kb on - strand, within Pf6N2E2_2547 at 3008.385 kb on - strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.429 kb on + strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.430 kb on - strand, within Pf6N2E2_2547 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.651 kb on + strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.652 kb on - strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.657 kb on + strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.658 kb on - strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.782 kb on + strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.783 kb on - strand, within Pf6N2E2_2548 at 3008.788 kb on + strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.789 kb on - strand, within Pf6N2E2_2548 at 3008.864 kb on + strand, within Pf6N2E2_2548 at 3008.864 kb on + strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3008.865 kb on - strand, within Pf6N2E2_2548 at 3009.125 kb on + strand, within Pf6N2E2_2548 at 3009.125 kb on + strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.179 kb on - strand, within Pf6N2E2_2548 at 3009.312 kb on + strand at 3009.342 kb on + strand at 3009.342 kb on + strand at 3009.353 kb on + strand at 3009.440 kb on + strand at 3009.462 kb on + strand at 3009.462 kb on + strand at 3009.463 kb on - strand at 3009.463 kb on - strand at 3009.463 kb on - strand at 3009.465 kb on + strand at 3009.465 kb on + strand at 3009.466 kb on - strand at 3009.466 kb on - strand at 3009.480 kb on + strand at 3009.481 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 3,007,202 + Pf6N2E2_2544 0.87 -1.1 3,007,202 + Pf6N2E2_2544 0.87 +0.2 3,007,202 + Pf6N2E2_2544 0.87 +2.3 3,007,203 - Pf6N2E2_2544 0.87 -0.6 3,007,203 - Pf6N2E2_2544 0.87 +1.0 3,007,203 - Pf6N2E2_2544 0.87 -2.5 3,007,203 - Pf6N2E2_2544 0.87 +2.3 3,007,517 + +1.0 3,007,518 - +1.6 3,007,518 - -0.0 3,007,518 - -0.6 3,007,518 - +0.6 3,007,756 + +0.7 3,007,757 - -0.4 3,007,813 + Pf6N2E2_2546 0.29 +2.3 3,007,813 + Pf6N2E2_2546 0.29 +0.8 3,007,813 + Pf6N2E2_2546 0.29 -0.3 3,007,904 + Pf6N2E2_2546 0.74 +1.4 3,007,904 + Pf6N2E2_2546 0.74 -0.0 3,007,932 + Pf6N2E2_2546 0.88 -0.5 3,007,932 + Pf6N2E2_2546 0.88 +0.2 3,007,947 + +1.0 3,007,947 + +0.6 3,007,947 + +1.0 3,007,947 + -1.2 3,007,947 + -2.7 3,007,948 - +0.3 3,007,948 - -0.1 3,007,948 - -0.4 3,008,044 - +0.3 3,008,044 - +2.3 3,008,104 - +1.2 3,008,104 - -0.0 3,008,238 + Pf6N2E2_2547 0.35 -3.1 3,008,238 + Pf6N2E2_2547 0.35 -1.5 3,008,238 + Pf6N2E2_2547 0.35 -0.9 3,008,238 + Pf6N2E2_2547 0.35 -0.1 3,008,238 + Pf6N2E2_2547 0.35 +2.1 3,008,238 + Pf6N2E2_2547 0.35 -1.7 3,008,239 - Pf6N2E2_2547 0.35 -0.0 3,008,239 - Pf6N2E2_2547 0.35 -1.6 3,008,240 + Pf6N2E2_2547 0.35 -0.7 3,008,240 + Pf6N2E2_2547 0.35 +0.9 3,008,240 + Pf6N2E2_2547 0.35 -1.0 3,008,240 + Pf6N2E2_2547 0.35 +0.6 3,008,240 + Pf6N2E2_2547 0.35 +0.0 3,008,240 + Pf6N2E2_2547 0.35 -0.6 3,008,240 + Pf6N2E2_2547 0.35 -1.2 3,008,240 + Pf6N2E2_2547 0.35 -1.3 3,008,240 + Pf6N2E2_2547 0.35 -0.0 3,008,240 + Pf6N2E2_2547 0.35 -0.6 3,008,241 - Pf6N2E2_2547 0.35 -0.0 3,008,241 - Pf6N2E2_2547 0.35 -1.7 3,008,241 - Pf6N2E2_2547 0.35 -1.6 3,008,241 - Pf6N2E2_2547 0.35 +0.8 3,008,253 - Pf6N2E2_2547 0.39 -0.2 3,008,261 + Pf6N2E2_2547 0.41 -0.8 3,008,276 + Pf6N2E2_2547 0.45 -0.2 3,008,276 + Pf6N2E2_2547 0.45 +1.0 3,008,277 - Pf6N2E2_2547 0.45 +1.0 3,008,385 - Pf6N2E2_2547 0.74 +0.8 3,008,429 + Pf6N2E2_2547 0.85 +1.7 3,008,429 + Pf6N2E2_2547 0.85 -1.8 3,008,429 + Pf6N2E2_2547 0.85 -1.2 3,008,429 + Pf6N2E2_2547 0.85 +0.1 3,008,430 - Pf6N2E2_2547 0.85 +0.3 3,008,430 - Pf6N2E2_2547 0.85 -1.3 3,008,430 - Pf6N2E2_2547 0.85 -0.2 3,008,430 - Pf6N2E2_2547 0.85 +2.0 3,008,651 + Pf6N2E2_2548 0.19 +2.0 3,008,651 + Pf6N2E2_2548 0.19 +0.9 3,008,651 + -0.0 3,008,651 + Pf6N2E2_2548 0.19 -0.5 3,008,651 + Pf6N2E2_2548 0.19 +1.0 3,008,651 + Pf6N2E2_2548 0.19 -0.0 3,008,651 + Pf6N2E2_2548 0.19 -0.8 3,008,651 + Pf6N2E2_2548 0.19 -0.5 3,008,651 + Pf6N2E2_2548 0.19 +1.8 3,008,652 - Pf6N2E2_2548 0.19 +0.5 3,008,652 - Pf6N2E2_2548 0.19 -2.8 3,008,652 - Pf6N2E2_2548 0.19 -3.5 3,008,652 - Pf6N2E2_2548 0.19 +0.3 3,008,652 - Pf6N2E2_2548 0.19 +0.5 3,008,652 - Pf6N2E2_2548 0.19 -0.6 3,008,652 - Pf6N2E2_2548 0.19 +0.2 3,008,657 + Pf6N2E2_2548 0.20 +0.4 3,008,657 + Pf6N2E2_2548 0.20 -1.3 3,008,657 + Pf6N2E2_2548 0.20 +0.5 3,008,658 - Pf6N2E2_2548 0.20 -0.8 3,008,658 - Pf6N2E2_2548 0.20 +0.3 3,008,658 - Pf6N2E2_2548 0.20 +0.6 3,008,782 + Pf6N2E2_2548 0.35 -0.6 3,008,782 + Pf6N2E2_2548 0.35 -0.3 3,008,782 + Pf6N2E2_2548 0.35 +0.7 3,008,782 + Pf6N2E2_2548 0.35 +0.5 3,008,782 + Pf6N2E2_2548 0.35 +0.3 3,008,783 - Pf6N2E2_2548 0.35 +1.5 3,008,783 - Pf6N2E2_2548 0.35 +1.0 3,008,783 - Pf6N2E2_2548 0.35 -0.4 3,008,783 - Pf6N2E2_2548 0.35 +2.1 3,008,788 + Pf6N2E2_2548 0.36 -3.1 3,008,789 - Pf6N2E2_2548 0.36 +0.7 3,008,789 - Pf6N2E2_2548 0.36 -1.0 3,008,789 - Pf6N2E2_2548 0.36 +2.1 3,008,789 - Pf6N2E2_2548 0.36 +0.8 3,008,864 + Pf6N2E2_2548 0.45 -2.1 3,008,864 + Pf6N2E2_2548 0.45 -1.3 3,008,865 - Pf6N2E2_2548 0.45 +1.6 3,008,865 - Pf6N2E2_2548 0.45 -0.7 3,008,865 - Pf6N2E2_2548 0.45 -0.7 3,009,125 + Pf6N2E2_2548 0.77 -1.7 3,009,125 + Pf6N2E2_2548 0.77 -0.2 3,009,179 - Pf6N2E2_2548 0.84 +0.6 3,009,179 - Pf6N2E2_2548 0.84 -0.6 3,009,179 - Pf6N2E2_2548 0.84 -1.0 3,009,179 - Pf6N2E2_2548 0.84 -0.2 3,009,312 + +1.3 3,009,342 + +0.4 3,009,342 + +0.7 3,009,353 + +0.3 3,009,440 + -0.4 3,009,462 + +0.3 3,009,462 + +0.0 3,009,463 - -2.0 3,009,463 - +0.7 3,009,463 - -0.0 3,009,465 + +1.2 3,009,465 + +0.3 3,009,466 - -1.3 3,009,466 - +0.7 3,009,480 + -0.3 3,009,481 - +0.4
Or see this region's nucleotide sequence