Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2450 and Pf6N2E2_2451 are separated by 54 nucleotides Pf6N2E2_2451 and Pf6N2E2_2452 are separated by 500 nucleotides Pf6N2E2_2452 and Pf6N2E2_2453 are separated by 139 nucleotides
Pf6N2E2_2450 - DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13), at 2,901,123 to 2,902,796
_2450
Pf6N2E2_2451 - hypothetical protein, at 2,902,851 to 2,903,003
_2451
Pf6N2E2_2452 - Spermidine synthase (EC 2.5.1.16), at 2,903,504 to 2,904,259
_2452
Pf6N2E2_2453 - 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54), at 2,904,399 to 2,905,748
_2453
Position (kb)
2903
2904
2905 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2902.809 kb on - strand at 2903.019 kb on - strand at 2903.062 kb on + strand at 2903.191 kb on - strand at 2903.482 kb on - strand at 2903.679 kb on + strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.680 kb on - strand, within Pf6N2E2_2452 at 2903.827 kb on + strand, within Pf6N2E2_2452 at 2903.830 kb on - strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.901 kb on + strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.902 kb on - strand, within Pf6N2E2_2452 at 2903.931 kb on + strand, within Pf6N2E2_2452 at 2904.022 kb on + strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.023 kb on - strand, within Pf6N2E2_2452 at 2904.097 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.099 kb on + strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.100 kb on - strand, within Pf6N2E2_2452 at 2904.106 kb on - strand, within Pf6N2E2_2452 at 2904.352 kb on + strand at 2904.353 kb on - strand at 2904.363 kb on + strand at 2904.391 kb on + strand at 2904.392 kb on - strand at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.874 kb on - strand, within Pf6N2E2_2453 at 2904.996 kb on + strand, within Pf6N2E2_2453 at 2905.119 kb on + strand, within Pf6N2E2_2453 at 2905.120 kb on - strand, within Pf6N2E2_2453 at 2905.162 kb on - strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.164 kb on + strand, within Pf6N2E2_2453 at 2905.165 kb on - strand, within Pf6N2E2_2453
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,902,809 - +0.5 2,903,019 - -0.1 2,903,062 + +1.4 2,903,191 - +1.1 2,903,482 - -0.5 2,903,679 + Pf6N2E2_2452 0.23 -0.7 2,903,680 - Pf6N2E2_2452 0.23 +0.2 2,903,680 - Pf6N2E2_2452 0.23 -0.7 2,903,827 + Pf6N2E2_2452 0.43 -0.7 2,903,830 - Pf6N2E2_2452 0.43 -1.1 2,903,901 + Pf6N2E2_2452 0.53 -0.3 2,903,901 + Pf6N2E2_2452 0.53 -1.9 2,903,901 + Pf6N2E2_2452 0.53 +0.1 2,903,902 - Pf6N2E2_2452 0.53 -0.5 2,903,902 - Pf6N2E2_2452 0.53 -1.7 2,903,902 - Pf6N2E2_2452 0.53 -0.7 2,903,931 + Pf6N2E2_2452 0.56 -1.1 2,904,022 + Pf6N2E2_2452 0.69 -2.4 2,904,023 - Pf6N2E2_2452 0.69 -1.4 2,904,023 - Pf6N2E2_2452 0.69 -0.0 2,904,023 - Pf6N2E2_2452 0.69 +0.9 2,904,097 + Pf6N2E2_2452 0.78 -1.7 2,904,099 + Pf6N2E2_2452 0.79 +0.0 2,904,099 + Pf6N2E2_2452 0.79 +1.0 2,904,099 + Pf6N2E2_2452 0.79 -0.1 2,904,099 + Pf6N2E2_2452 0.79 -2.9 2,904,099 + Pf6N2E2_2452 0.79 -1.1 2,904,099 + Pf6N2E2_2452 0.79 +0.6 2,904,100 - Pf6N2E2_2452 0.79 -0.1 2,904,100 - Pf6N2E2_2452 0.79 -0.7 2,904,100 - Pf6N2E2_2452 0.79 +0.2 2,904,100 - Pf6N2E2_2452 0.79 -1.9 2,904,100 - Pf6N2E2_2452 0.79 -2.3 2,904,106 - Pf6N2E2_2452 0.80 -0.3 2,904,352 + +0.9 2,904,353 - +0.5 2,904,363 + +0.3 2,904,391 + +1.4 2,904,392 - +0.9 2,904,874 - Pf6N2E2_2453 0.35 -2.9 2,904,874 - Pf6N2E2_2453 0.35 +0.4 2,904,996 + Pf6N2E2_2453 0.44 -0.7 2,905,119 + Pf6N2E2_2453 0.53 -1.4 2,905,120 - Pf6N2E2_2453 0.53 -0.3 2,905,162 - Pf6N2E2_2453 0.57 -0.5 2,905,164 + Pf6N2E2_2453 0.57 +0.9 2,905,164 + Pf6N2E2_2453 0.57 +0.8 2,905,164 + Pf6N2E2_2453 0.57 +1.0 2,905,165 - Pf6N2E2_2453 0.57 -2.0
Or see this region's nucleotide sequence