Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_224 and Pf6N2E2_225 are separated by 88 nucleotides Pf6N2E2_225 and Pf6N2E2_226 are separated by 74 nucleotides Pf6N2E2_226 and Pf6N2E2_227 are separated by 22 nucleotides
Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216
_224
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Pf6N2E2_226 - 2,3-dihydroxybiphenyl 1,2-dioxygenase, at 287,858 to 288,706
_226
Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375
_227
Position (kb) 286
287
288 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.697 kb on - strand, within Pf6N2E2_224 at 285.748 kb on + strand, within Pf6N2E2_224 at 285.952 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.113 kb on + strand, within Pf6N2E2_224 at 286.114 kb on - strand, within Pf6N2E2_224 at 286.205 kb on + strand at 286.227 kb on + strand at 286.228 kb on - strand at 286.231 kb on + strand at 286.232 kb on - strand at 286.232 kb on - strand at 286.234 kb on + strand at 286.235 kb on - strand at 286.376 kb on - strand at 286.376 kb on - strand at 286.461 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.865 kb on - strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.377 kb on + strand, within Pf6N2E2_225 at 287.617 kb on + strand, within Pf6N2E2_225 at 287.618 kb on - strand, within Pf6N2E2_225 at 287.737 kb on + strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.738 kb on - strand at 287.792 kb on - strand at 287.821 kb on - strand at 287.821 kb on - strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.898 kb on + strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 287.899 kb on - strand at 288.109 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.256 kb on - strand, within Pf6N2E2_226 at 288.362 kb on + strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.363 kb on - strand, within Pf6N2E2_226 at 288.666 kb on + strand at 288.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 285,423 + Pf6N2E2_224 0.21 -0.2 285,423 + Pf6N2E2_224 0.21 -0.8 285,423 + Pf6N2E2_224 0.21 +0.8 285,424 - Pf6N2E2_224 0.21 -0.2 285,424 - Pf6N2E2_224 0.21 -1.4 285,697 - Pf6N2E2_224 0.48 -2.2 285,748 + Pf6N2E2_224 0.53 -0.2 285,952 + Pf6N2E2_224 0.73 +0.7 286,109 + Pf6N2E2_224 0.89 +0.6 286,109 + Pf6N2E2_224 0.89 +0.8 286,110 - Pf6N2E2_224 0.89 -0.2 286,110 - Pf6N2E2_224 0.89 -0.3 286,110 - Pf6N2E2_224 0.89 +0.5 286,110 - Pf6N2E2_224 0.89 -1.2 286,110 - Pf6N2E2_224 0.89 +0.9 286,111 + Pf6N2E2_224 0.89 -0.8 286,111 + Pf6N2E2_224 0.89 -0.9 286,112 - Pf6N2E2_224 0.89 -1.3 286,112 - Pf6N2E2_224 0.89 -0.0 286,113 + Pf6N2E2_224 0.90 +1.9 286,114 - Pf6N2E2_224 0.90 -2.5 286,205 + +0.3 286,227 + -2.8 286,228 - -0.6 286,231 + +0.2 286,232 - -1.9 286,232 - +0.6 286,234 + +0.3 286,235 - +0.3 286,376 - -0.8 286,376 - -1.8 286,461 - Pf6N2E2_225 0.11 +1.5 286,651 - Pf6N2E2_225 0.23 +0.6 286,651 - Pf6N2E2_225 0.23 +2.6 286,864 + Pf6N2E2_225 0.38 +0.3 286,864 + Pf6N2E2_225 0.38 +1.6 286,865 - Pf6N2E2_225 0.38 -0.1 287,377 + Pf6N2E2_225 0.72 +1.0 287,377 + Pf6N2E2_225 0.72 +0.7 287,377 + Pf6N2E2_225 0.72 +1.2 287,617 + Pf6N2E2_225 0.89 -1.2 287,618 - Pf6N2E2_225 0.89 -1.0 287,737 + -0.8 287,738 - -0.8 287,738 - +0.3 287,738 - +0.5 287,738 - -1.5 287,738 - +0.8 287,792 - +0.8 287,821 - +1.9 287,821 - -0.2 287,898 + +1.2 287,898 + +2.4 287,898 + -0.5 287,899 - +0.4 287,899 - +0.7 287,899 - +0.1 287,899 - +0.2 287,899 - +0.1 288,109 - Pf6N2E2_226 0.30 -0.9 288,256 - Pf6N2E2_226 0.47 -1.9 288,256 - Pf6N2E2_226 0.47 +1.0 288,362 + Pf6N2E2_226 0.59 -0.2 288,363 - Pf6N2E2_226 0.59 +0.4 288,363 - Pf6N2E2_226 0.59 +1.9 288,666 + +0.6 288,730 + -0.8
Or see this region's nucleotide sequence