Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2019 and Pf6N2E2_6108 are separated by 305 nucleotides Pf6N2E2_6108 and Pf6N2E2_2020 are separated by 63 nucleotides Pf6N2E2_2020 and Pf6N2E2_2021 are separated by 32 nucleotides
Pf6N2E2_2019 - hypothetical protein, at 2,404,657 to 2,408,535
_2019
Pf6N2E2_6108 - tRNA-Gly-GCC, at 2,408,841 to 2,408,913
_6108
Pf6N2E2_2020 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 2,408,977 to 2,409,537
_2020
Pf6N2E2_2021 - Excinuclease ABC subunit C, at 2,409,570 to 2,411,327
_2021
Position (kb)
2408
2409
2410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2408.013 kb on + strand, within Pf6N2E2_2019 at 2408.013 kb on + strand, within Pf6N2E2_2019 at 2408.013 kb on + strand, within Pf6N2E2_2019 at 2408.013 kb on + strand, within Pf6N2E2_2019 at 2408.013 kb on + strand, within Pf6N2E2_2019 at 2408.024 kb on + strand, within Pf6N2E2_2019 at 2408.025 kb on - strand, within Pf6N2E2_2019 at 2408.035 kb on + strand, within Pf6N2E2_2019 at 2408.036 kb on - strand, within Pf6N2E2_2019 at 2408.036 kb on - strand, within Pf6N2E2_2019 at 2408.087 kb on + strand, within Pf6N2E2_2019 at 2408.087 kb on + strand, within Pf6N2E2_2019 at 2408.087 kb on + strand, within Pf6N2E2_2019 at 2408.096 kb on + strand, within Pf6N2E2_2019 at 2408.104 kb on + strand, within Pf6N2E2_2019 at 2408.104 kb on + strand, within Pf6N2E2_2019 at 2408.104 kb on + strand, within Pf6N2E2_2019 at 2408.104 kb on + strand, within Pf6N2E2_2019 at 2408.105 kb on - strand at 2408.105 kb on - strand, within Pf6N2E2_2019 at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.168 kb on + strand at 2408.196 kb on + strand at 2408.294 kb on + strand at 2408.294 kb on + strand at 2408.294 kb on + strand at 2408.295 kb on - strand at 2408.335 kb on - strand at 2408.369 kb on + strand at 2408.377 kb on + strand at 2408.378 kb on - strand at 2408.414 kb on + strand at 2408.415 kb on - strand at 2408.415 kb on - strand at 2408.460 kb on - strand at 2408.484 kb on - strand at 2408.513 kb on - strand at 2408.533 kb on + strand at 2408.533 kb on + strand at 2408.534 kb on - strand at 2408.536 kb on + strand at 2408.536 kb on + strand at 2408.536 kb on + strand at 2408.536 kb on + strand at 2408.536 kb on + strand at 2408.537 kb on - strand at 2408.537 kb on - strand at 2408.537 kb on - strand at 2408.543 kb on + strand at 2408.620 kb on + strand at 2408.635 kb on + strand at 2408.635 kb on + strand at 2408.636 kb on - strand at 2408.676 kb on + strand at 2408.680 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.712 kb on + strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.713 kb on - strand at 2408.715 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.776 kb on + strand at 2408.777 kb on - strand at 2408.935 kb on + strand at 2408.935 kb on + strand at 2408.936 kb on - strand at 2408.936 kb on - strand at 2409.579 kb on - strand at 2409.593 kb on - strand at 2409.593 kb on - strand at 2409.614 kb on + strand at 2409.737 kb on + strand at 2409.737 kb on + strand at 2409.738 kb on - strand at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2409.877 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.081 kb on + strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.082 kb on - strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.114 kb on + strand, within Pf6N2E2_2021 at 2410.204 kb on + strand, within Pf6N2E2_2021 at 2410.205 kb on - strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.486 kb on + strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.487 kb on - strand, within Pf6N2E2_2021 at 2410.489 kb on + strand, within Pf6N2E2_2021
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,408,013 + Pf6N2E2_2019 0.87 +0.8 2,408,013 + Pf6N2E2_2019 0.87 -0.1 2,408,013 + Pf6N2E2_2019 0.87 -1.2 2,408,013 + Pf6N2E2_2019 0.87 -0.5 2,408,013 + Pf6N2E2_2019 0.87 +0.7 2,408,024 + Pf6N2E2_2019 0.87 -1.6 2,408,025 - Pf6N2E2_2019 0.87 -0.2 2,408,035 + Pf6N2E2_2019 0.87 -0.4 2,408,036 - Pf6N2E2_2019 0.87 +0.8 2,408,036 - Pf6N2E2_2019 0.87 -0.2 2,408,087 + Pf6N2E2_2019 0.88 -0.2 2,408,087 + Pf6N2E2_2019 0.88 -2.0 2,408,087 + Pf6N2E2_2019 0.88 -2.0 2,408,096 + Pf6N2E2_2019 0.89 +1.3 2,408,104 + Pf6N2E2_2019 0.89 +0.0 2,408,104 + Pf6N2E2_2019 0.89 +1.3 2,408,104 + Pf6N2E2_2019 0.89 -0.2 2,408,104 + Pf6N2E2_2019 0.89 +1.2 2,408,105 - +0.8 2,408,105 - Pf6N2E2_2019 0.89 -0.3 2,408,168 + +0.3 2,408,168 + +1.2 2,408,168 + +0.1 2,408,168 + -0.8 2,408,168 + -1.5 2,408,168 + -2.7 2,408,168 + -1.1 2,408,168 + +1.4 2,408,196 + -1.9 2,408,294 + +0.3 2,408,294 + +0.8 2,408,294 + -0.8 2,408,295 - -1.5 2,408,335 - +1.4 2,408,369 + -0.2 2,408,377 + +2.0 2,408,378 - -0.8 2,408,414 + +1.4 2,408,415 - +1.0 2,408,415 - +0.8 2,408,460 - +0.1 2,408,484 - +1.0 2,408,513 - +0.4 2,408,533 + -2.5 2,408,533 + +0.8 2,408,534 - +0.9 2,408,536 + -0.8 2,408,536 + -0.2 2,408,536 + -0.5 2,408,536 + -0.9 2,408,536 + +0.8 2,408,537 - -2.4 2,408,537 - +1.5 2,408,537 - +0.5 2,408,543 + -0.5 2,408,620 + +2.3 2,408,635 + +0.0 2,408,635 + -1.8 2,408,636 - +1.2 2,408,676 + -1.9 2,408,680 + +0.8 2,408,712 + +1.3 2,408,712 + +0.8 2,408,712 + -1.2 2,408,712 + -0.8 2,408,712 + -1.0 2,408,712 + -0.8 2,408,712 + -0.2 2,408,713 - -0.8 2,408,713 - +0.4 2,408,713 - +2.8 2,408,713 - -0.4 2,408,715 + -2.8 2,408,776 + +0.4 2,408,776 + +1.0 2,408,776 + -2.4 2,408,776 + +0.8 2,408,776 + -1.0 2,408,776 + -1.7 2,408,777 - +1.9 2,408,935 + -2.8 2,408,935 + +2.7 2,408,936 - -0.0 2,408,936 - -3.5 2,409,579 - +0.8 2,409,593 - -2.0 2,409,593 - +1.6 2,409,614 + -0.0 2,409,737 + +0.3 2,409,737 + +0.8 2,409,738 - -1.5 2,409,877 + Pf6N2E2_2021 0.17 +0.8 2,409,877 + Pf6N2E2_2021 0.17 -1.4 2,410,081 + Pf6N2E2_2021 0.29 +0.2 2,410,081 + Pf6N2E2_2021 0.29 +0.8 2,410,081 + Pf6N2E2_2021 0.29 -1.2 2,410,082 - Pf6N2E2_2021 0.29 -0.9 2,410,082 - Pf6N2E2_2021 0.29 -1.5 2,410,114 + Pf6N2E2_2021 0.31 -3.3 2,410,114 + Pf6N2E2_2021 0.31 -0.8 2,410,204 + Pf6N2E2_2021 0.36 -1.2 2,410,205 - Pf6N2E2_2021 0.36 +0.8 2,410,486 + Pf6N2E2_2021 0.52 +1.6 2,410,486 + Pf6N2E2_2021 0.52 +0.2 2,410,487 - Pf6N2E2_2021 0.52 +0.3 2,410,487 - Pf6N2E2_2021 0.52 -0.2 2,410,487 - Pf6N2E2_2021 0.52 +0.8 2,410,489 + Pf6N2E2_2021 0.52 -0.8
Or see this region's nucleotide sequence