Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1768 and Pf6N2E2_1769 overlap by 4 nucleotides Pf6N2E2_1769 and Pf6N2E2_1770 are separated by 44 nucleotides Pf6N2E2_1770 and Pf6N2E2_1771 are separated by 56 nucleotides
Pf6N2E2_1768 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,089,558 to 2,090,982
_1768
Pf6N2E2_1769 - Nucleoside-diphosphate-sugar epimerases, at 2,090,979 to 2,091,320
_1769
Pf6N2E2_1770 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 2,091,365 to 2,092,438
_1770
Pf6N2E2_1771 - Response regulator, at 2,092,495 to 2,093,475
_1771
Position (kb)
2091
2092
2093 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.425 kb on + strand, within Pf6N2E2_1768 at 2090.426 kb on - strand, within Pf6N2E2_1768 at 2090.476 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.696 kb on + strand, within Pf6N2E2_1768 at 2090.843 kb on - strand at 2090.883 kb on - strand at 2090.883 kb on - strand at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.220 kb on + strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.221 kb on - strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.224 kb on + strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.225 kb on - strand, within Pf6N2E2_1769 at 2091.280 kb on + strand, within Pf6N2E2_1769 at 2091.303 kb on + strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.304 kb on - strand at 2091.308 kb on - strand at 2091.388 kb on - strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.434 kb on + strand at 2091.449 kb on + strand at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.507 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.729 kb on - strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.792 kb on + strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.793 kb on - strand, within Pf6N2E2_1770 at 2091.905 kb on + strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.906 kb on - strand, within Pf6N2E2_1770 at 2091.945 kb on - strand, within Pf6N2E2_1770 at 2091.983 kb on + strand, within Pf6N2E2_1770 at 2091.993 kb on - strand, within Pf6N2E2_1770 at 2091.993 kb on - strand, within Pf6N2E2_1770 at 2092.010 kb on + strand, within Pf6N2E2_1770 at 2092.010 kb on + strand, within Pf6N2E2_1770 at 2092.011 kb on - strand, within Pf6N2E2_1770 at 2092.122 kb on - strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.217 kb on + strand, within Pf6N2E2_1770 at 2092.218 kb on - strand, within Pf6N2E2_1770 at 2092.266 kb on - strand, within Pf6N2E2_1770 at 2092.331 kb on + strand at 2092.365 kb on + strand at 2092.423 kb on + strand at 2092.424 kb on - strand at 2092.532 kb on - strand at 2092.639 kb on - strand, within Pf6N2E2_1771 at 2092.641 kb on - strand, within Pf6N2E2_1771 at 2092.641 kb on - strand, within Pf6N2E2_1771 at 2092.657 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.668 kb on - strand, within Pf6N2E2_1771 at 2092.903 kb on + strand, within Pf6N2E2_1771 at 2092.903 kb on + strand, within Pf6N2E2_1771 at 2092.904 kb on - strand, within Pf6N2E2_1771 at 2093.014 kb on - strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.022 kb on + strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.023 kb on - strand, within Pf6N2E2_1771 at 2093.049 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.088 kb on - strand, within Pf6N2E2_1771 at 2093.187 kb on + strand, within Pf6N2E2_1771 at 2093.187 kb on + strand, within Pf6N2E2_1771 at 2093.188 kb on - strand, within Pf6N2E2_1771 at 2093.329 kb on - strand, within Pf6N2E2_1771 at 2093.329 kb on - strand, within Pf6N2E2_1771
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,090,425 + Pf6N2E2_1768 0.61 -0.4 2,090,425 + Pf6N2E2_1768 0.61 -1.0 2,090,426 - Pf6N2E2_1768 0.61 +0.4 2,090,476 + Pf6N2E2_1768 0.64 +0.3 2,090,696 + Pf6N2E2_1768 0.80 +0.6 2,090,696 + Pf6N2E2_1768 0.80 +0.0 2,090,843 - +1.4 2,090,883 - +0.0 2,090,883 - +0.5 2,091,220 + Pf6N2E2_1769 0.70 +0.6 2,091,220 + Pf6N2E2_1769 0.70 +0.6 2,091,220 + Pf6N2E2_1769 0.70 -1.6 2,091,220 + Pf6N2E2_1769 0.70 +0.6 2,091,220 + Pf6N2E2_1769 0.70 +2.2 2,091,220 + Pf6N2E2_1769 0.70 -0.4 2,091,221 - Pf6N2E2_1769 0.71 -0.7 2,091,221 - Pf6N2E2_1769 0.71 +0.2 2,091,221 - Pf6N2E2_1769 0.71 +2.1 2,091,221 - Pf6N2E2_1769 0.71 +1.2 2,091,224 + Pf6N2E2_1769 0.72 -1.0 2,091,224 + Pf6N2E2_1769 0.72 -1.0 2,091,225 - Pf6N2E2_1769 0.72 -0.6 2,091,225 - Pf6N2E2_1769 0.72 -1.7 2,091,225 - Pf6N2E2_1769 0.72 +0.3 2,091,225 - Pf6N2E2_1769 0.72 -1.4 2,091,280 + Pf6N2E2_1769 0.88 +1.4 2,091,303 + +0.4 2,091,304 - -2.4 2,091,304 - +0.6 2,091,304 - -0.1 2,091,308 - +1.6 2,091,388 - -0.4 2,091,434 + +0.4 2,091,434 + -0.4 2,091,434 + -2.7 2,091,449 + -1.0 2,091,507 - Pf6N2E2_1770 0.13 +2.3 2,091,507 - Pf6N2E2_1770 0.13 +0.6 2,091,729 - Pf6N2E2_1770 0.34 +0.0 2,091,729 - Pf6N2E2_1770 0.34 -0.4 2,091,792 + Pf6N2E2_1770 0.40 -0.4 2,091,792 + Pf6N2E2_1770 0.40 +0.6 2,091,792 + Pf6N2E2_1770 0.40 -0.5 2,091,793 - Pf6N2E2_1770 0.40 +0.5 2,091,793 - Pf6N2E2_1770 0.40 +0.0 2,091,905 + Pf6N2E2_1770 0.50 -1.4 2,091,906 - Pf6N2E2_1770 0.50 -1.5 2,091,906 - Pf6N2E2_1770 0.50 +1.0 2,091,906 - Pf6N2E2_1770 0.50 +1.0 2,091,945 - Pf6N2E2_1770 0.54 -2.1 2,091,983 + Pf6N2E2_1770 0.58 +1.0 2,091,993 - Pf6N2E2_1770 0.58 -0.4 2,091,993 - Pf6N2E2_1770 0.58 +0.5 2,092,010 + Pf6N2E2_1770 0.60 -2.8 2,092,010 + Pf6N2E2_1770 0.60 +1.2 2,092,011 - Pf6N2E2_1770 0.60 +0.1 2,092,122 - Pf6N2E2_1770 0.70 -2.7 2,092,217 + Pf6N2E2_1770 0.79 -0.5 2,092,217 + Pf6N2E2_1770 0.79 +1.7 2,092,217 + Pf6N2E2_1770 0.79 -1.1 2,092,217 + Pf6N2E2_1770 0.79 -0.5 2,092,217 + Pf6N2E2_1770 0.79 -0.8 2,092,218 - Pf6N2E2_1770 0.79 -1.0 2,092,266 - Pf6N2E2_1770 0.84 -1.7 2,092,331 + -1.6 2,092,365 + -0.4 2,092,423 + +3.4 2,092,424 - +1.3 2,092,532 - +0.6 2,092,639 - Pf6N2E2_1771 0.15 -0.4 2,092,641 - Pf6N2E2_1771 0.15 -2.0 2,092,641 - Pf6N2E2_1771 0.15 +0.7 2,092,657 - Pf6N2E2_1771 0.17 -0.4 2,092,668 - Pf6N2E2_1771 0.18 -0.3 2,092,668 - Pf6N2E2_1771 0.18 +1.6 2,092,668 - Pf6N2E2_1771 0.18 -2.8 2,092,668 - Pf6N2E2_1771 0.18 +2.2 2,092,903 + Pf6N2E2_1771 0.42 -1.5 2,092,903 + Pf6N2E2_1771 0.42 -1.0 2,092,904 - Pf6N2E2_1771 0.42 +0.0 2,093,014 - Pf6N2E2_1771 0.53 +0.1 2,093,022 + Pf6N2E2_1771 0.54 +0.9 2,093,022 + Pf6N2E2_1771 0.54 -1.0 2,093,022 + Pf6N2E2_1771 0.54 +1.1 2,093,022 + Pf6N2E2_1771 0.54 +0.9 2,093,022 + Pf6N2E2_1771 0.54 -1.0 2,093,022 + Pf6N2E2_1771 0.54 -0.1 2,093,023 - Pf6N2E2_1771 0.54 +0.4 2,093,023 - Pf6N2E2_1771 0.54 -2.6 2,093,023 - Pf6N2E2_1771 0.54 +2.0 2,093,023 - Pf6N2E2_1771 0.54 -0.4 2,093,049 - Pf6N2E2_1771 0.56 -0.4 2,093,088 - Pf6N2E2_1771 0.60 -3.1 2,093,088 - Pf6N2E2_1771 0.60 -0.1 2,093,088 - Pf6N2E2_1771 0.60 -0.7 2,093,187 + Pf6N2E2_1771 0.71 +0.6 2,093,187 + Pf6N2E2_1771 0.71 +2.2 2,093,188 - Pf6N2E2_1771 0.71 +0.6 2,093,329 - Pf6N2E2_1771 0.85 -0.7 2,093,329 - Pf6N2E2_1771 0.85 -1.0
Or see this region's nucleotide sequence