Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1744 and Pf6N2E2_1745 are separated by 143 nucleotides Pf6N2E2_1745 and Pf6N2E2_1746 are separated by 12 nucleotides Pf6N2E2_1746 and Pf6N2E2_1747 are separated by 73 nucleotides
Pf6N2E2_1744 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,057,416 to 2,058,468
_1744
Pf6N2E2_1745 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,058,612 to 2,059,793
_1745
Pf6N2E2_1746 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 2,059,806 to 2,060,624
_1746
Pf6N2E2_1747 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,060,698 to 2,061,432
_1747
Position (kb)
2058
2059
2060 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2057.821 kb on + strand, within Pf6N2E2_1744 at 2058.116 kb on - strand, within Pf6N2E2_1744 at 2058.185 kb on - strand, within Pf6N2E2_1744 at 2058.238 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.253 kb on + strand, within Pf6N2E2_1744 at 2058.466 kb on + strand at 2058.467 kb on - strand at 2058.540 kb on - strand at 2058.640 kb on - strand at 2058.640 kb on - strand at 2058.696 kb on - strand at 2058.737 kb on - strand, within Pf6N2E2_1745 at 2058.828 kb on - strand, within Pf6N2E2_1745 at 2059.048 kb on - strand, within Pf6N2E2_1745 at 2059.119 kb on + strand, within Pf6N2E2_1745 at 2059.150 kb on + strand, within Pf6N2E2_1745 at 2059.151 kb on - strand, within Pf6N2E2_1745 at 2059.151 kb on - strand, within Pf6N2E2_1745 at 2059.399 kb on - strand, within Pf6N2E2_1745 at 2059.447 kb on - strand, within Pf6N2E2_1745 at 2059.447 kb on - strand, within Pf6N2E2_1745 at 2059.533 kb on + strand, within Pf6N2E2_1745 at 2059.534 kb on - strand, within Pf6N2E2_1745 at 2059.600 kb on - strand, within Pf6N2E2_1745 at 2059.603 kb on - strand, within Pf6N2E2_1745 at 2059.665 kb on + strand, within Pf6N2E2_1745 at 2059.666 kb on - strand, within Pf6N2E2_1745 at 2059.740 kb on + strand at 2059.740 kb on + strand at 2059.740 kb on + strand at 2059.791 kb on + strand at 2059.873 kb on + strand at 2059.873 kb on + strand at 2059.907 kb on + strand, within Pf6N2E2_1746 at 2059.907 kb on + strand, within Pf6N2E2_1746 at 2059.969 kb on - strand, within Pf6N2E2_1746 at 2060.116 kb on + strand, within Pf6N2E2_1746 at 2060.117 kb on - strand, within Pf6N2E2_1746 at 2060.175 kb on + strand, within Pf6N2E2_1746 at 2060.175 kb on + strand, within Pf6N2E2_1746 at 2060.175 kb on + strand, within Pf6N2E2_1746 at 2060.176 kb on - strand, within Pf6N2E2_1746 at 2060.701 kb on + strand at 2060.701 kb on + strand at 2060.701 kb on + strand at 2060.702 kb on - strand at 2060.778 kb on - strand, within Pf6N2E2_1747
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 2,057,821 + Pf6N2E2_1744 0.38 -1.4 2,057,821 + Pf6N2E2_1744 0.38 +1.0 2,058,116 - Pf6N2E2_1744 0.66 +0.1 2,058,185 - Pf6N2E2_1744 0.73 -1.0 2,058,238 + Pf6N2E2_1744 0.78 +0.6 2,058,253 + Pf6N2E2_1744 0.79 +1.1 2,058,253 + Pf6N2E2_1744 0.79 -1.4 2,058,253 + Pf6N2E2_1744 0.79 +0.9 2,058,466 + +0.7 2,058,467 - +0.9 2,058,540 - +0.0 2,058,640 - -0.7 2,058,640 - +0.3 2,058,696 - +0.1 2,058,737 - Pf6N2E2_1745 0.11 +0.8 2,058,828 - Pf6N2E2_1745 0.18 +0.2 2,059,048 - Pf6N2E2_1745 0.37 -0.4 2,059,119 + Pf6N2E2_1745 0.43 -0.4 2,059,150 + Pf6N2E2_1745 0.46 +0.7 2,059,151 - Pf6N2E2_1745 0.46 +1.3 2,059,151 - Pf6N2E2_1745 0.46 -1.7 2,059,399 - Pf6N2E2_1745 0.67 +1.3 2,059,447 - Pf6N2E2_1745 0.71 +1.3 2,059,447 - Pf6N2E2_1745 0.71 +0.1 2,059,533 + Pf6N2E2_1745 0.78 -0.4 2,059,534 - Pf6N2E2_1745 0.78 -1.0 2,059,600 - Pf6N2E2_1745 0.84 +0.9 2,059,603 - Pf6N2E2_1745 0.84 +0.1 2,059,665 + Pf6N2E2_1745 0.89 +1.3 2,059,666 - Pf6N2E2_1745 0.89 +2.1 2,059,740 + -0.1 2,059,740 + -1.7 2,059,740 + +0.3 2,059,791 + -1.7 2,059,873 + +2.9 2,059,873 + +1.0 2,059,907 + Pf6N2E2_1746 0.12 -1.7 2,059,907 + Pf6N2E2_1746 0.12 -1.9 2,059,969 - Pf6N2E2_1746 0.20 +1.6 2,060,116 + Pf6N2E2_1746 0.38 -0.9 2,060,117 - Pf6N2E2_1746 0.38 -1.0 2,060,175 + Pf6N2E2_1746 0.45 +0.9 2,060,175 + Pf6N2E2_1746 0.45 +1.7 2,060,175 + Pf6N2E2_1746 0.45 +1.3 2,060,176 - Pf6N2E2_1746 0.45 -1.0 2,060,701 + -1.4 2,060,701 + +0.6 2,060,701 + -0.4 2,060,702 - -0.2 2,060,778 - Pf6N2E2_1747 0.11 +0.6
Or see this region's nucleotide sequence