Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1683 and Pf6N2E2_1684 are separated by 147 nucleotides Pf6N2E2_1684 and Pf6N2E2_1685 are separated by 64 nucleotides
Pf6N2E2_1683 - Fe2+-dicitrate sensor, membrane component, at 1,990,742 to 1,991,719
_1683
Pf6N2E2_1684 - Ferrichrome-iron receptor, at 1,991,867 to 1,994,230
_1684
Pf6N2E2_1685 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 1,994,295 to 1,995,482
_1685
Position (kb)
1991
1992
1993
1994
1995 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1991.170 kb on - strand, within Pf6N2E2_1683 at 1991.349 kb on + strand, within Pf6N2E2_1683 at 1991.733 kb on - strand at 1992.090 kb on - strand at 1992.118 kb on - strand, within Pf6N2E2_1684 at 1992.165 kb on - strand, within Pf6N2E2_1684 at 1992.244 kb on + strand, within Pf6N2E2_1684 at 1992.335 kb on + strand, within Pf6N2E2_1684 at 1992.335 kb on + strand, within Pf6N2E2_1684 at 1992.336 kb on - strand, within Pf6N2E2_1684 at 1992.336 kb on - strand, within Pf6N2E2_1684 at 1992.507 kb on - strand, within Pf6N2E2_1684 at 1992.507 kb on - strand, within Pf6N2E2_1684 at 1992.543 kb on - strand, within Pf6N2E2_1684 at 1992.588 kb on - strand, within Pf6N2E2_1684 at 1992.840 kb on - strand, within Pf6N2E2_1684 at 1992.840 kb on - strand, within Pf6N2E2_1684 at 1993.158 kb on - strand, within Pf6N2E2_1684 at 1993.158 kb on - strand, within Pf6N2E2_1684 at 1993.265 kb on - strand, within Pf6N2E2_1684 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.314 kb on - strand, within Pf6N2E2_1684 at 1993.478 kb on + strand, within Pf6N2E2_1684 at 1993.478 kb on + strand, within Pf6N2E2_1684 at 1993.479 kb on - strand, within Pf6N2E2_1684 at 1993.479 kb on - strand, within Pf6N2E2_1684 at 1993.506 kb on - strand, within Pf6N2E2_1684 at 1993.572 kb on - strand, within Pf6N2E2_1684 at 1993.574 kb on + strand, within Pf6N2E2_1684 at 1993.574 kb on + strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.575 kb on - strand, within Pf6N2E2_1684 at 1993.580 kb on + strand, within Pf6N2E2_1684 at 1993.580 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.652 kb on + strand, within Pf6N2E2_1684 at 1993.653 kb on - strand, within Pf6N2E2_1684 at 1993.692 kb on - strand, within Pf6N2E2_1684 at 1993.692 kb on - strand, within Pf6N2E2_1684 at 1993.857 kb on + strand, within Pf6N2E2_1684 at 1993.907 kb on - strand, within Pf6N2E2_1684 at 1994.168 kb on + strand at 1994.169 kb on - strand at 1994.585 kb on + strand, within Pf6N2E2_1685 at 1994.590 kb on - strand, within Pf6N2E2_1685 at 1994.745 kb on + strand, within Pf6N2E2_1685 at 1994.784 kb on + strand, within Pf6N2E2_1685 at 1994.785 kb on - strand, within Pf6N2E2_1685 at 1994.785 kb on - strand, within Pf6N2E2_1685 at 1994.976 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,991,170 - Pf6N2E2_1683 0.44 +1.3 1,991,349 + Pf6N2E2_1683 0.62 +0.3 1,991,733 - -1.0 1,992,090 - -0.1 1,992,118 - Pf6N2E2_1684 0.11 -1.0 1,992,165 - Pf6N2E2_1684 0.13 +1.3 1,992,244 + Pf6N2E2_1684 0.16 +2.1 1,992,335 + Pf6N2E2_1684 0.20 +1.2 1,992,335 + Pf6N2E2_1684 0.20 +2.2 1,992,336 - Pf6N2E2_1684 0.20 +1.4 1,992,336 - Pf6N2E2_1684 0.20 -0.8 1,992,507 - Pf6N2E2_1684 0.27 +0.2 1,992,507 - Pf6N2E2_1684 0.27 +0.6 1,992,543 - Pf6N2E2_1684 0.29 -0.4 1,992,588 - Pf6N2E2_1684 0.30 -0.8 1,992,840 - Pf6N2E2_1684 0.41 -1.4 1,992,840 - Pf6N2E2_1684 0.41 +0.8 1,993,158 - Pf6N2E2_1684 0.55 -1.8 1,993,158 - Pf6N2E2_1684 0.55 +0.2 1,993,265 - Pf6N2E2_1684 0.59 +1.1 1,993,314 - Pf6N2E2_1684 0.61 -1.0 1,993,314 - Pf6N2E2_1684 0.61 +0.0 1,993,314 - Pf6N2E2_1684 0.61 -0.4 1,993,478 + Pf6N2E2_1684 0.68 +0.1 1,993,478 + Pf6N2E2_1684 0.68 -0.9 1,993,479 - Pf6N2E2_1684 0.68 -1.8 1,993,479 - Pf6N2E2_1684 0.68 +0.7 1,993,506 - Pf6N2E2_1684 0.69 +0.8 1,993,572 - Pf6N2E2_1684 0.72 +1.1 1,993,574 + Pf6N2E2_1684 0.72 -0.4 1,993,574 + Pf6N2E2_1684 0.72 +1.1 1,993,575 - Pf6N2E2_1684 0.72 +0.4 1,993,575 - Pf6N2E2_1684 0.72 +0.5 1,993,575 - Pf6N2E2_1684 0.72 +0.6 1,993,580 + Pf6N2E2_1684 0.72 +0.6 1,993,580 + Pf6N2E2_1684 0.72 -1.0 1,993,652 + Pf6N2E2_1684 0.76 -0.4 1,993,652 + Pf6N2E2_1684 0.76 +1.3 1,993,652 + Pf6N2E2_1684 0.76 +1.5 1,993,653 - Pf6N2E2_1684 0.76 -0.4 1,993,692 - Pf6N2E2_1684 0.77 +0.2 1,993,692 - Pf6N2E2_1684 0.77 -0.6 1,993,857 + Pf6N2E2_1684 0.84 -0.9 1,993,907 - Pf6N2E2_1684 0.86 +2.6 1,994,168 + +1.7 1,994,169 - -1.7 1,994,585 + Pf6N2E2_1685 0.24 +0.6 1,994,590 - Pf6N2E2_1685 0.25 +2.1 1,994,745 + Pf6N2E2_1685 0.38 -1.4 1,994,784 + Pf6N2E2_1685 0.41 +0.8 1,994,785 - Pf6N2E2_1685 0.41 -0.0 1,994,785 - Pf6N2E2_1685 0.41 -0.0 1,994,976 + +0.6
Or see this region's nucleotide sequence