Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1684

Experiment: RCH2 defined with Cobalt chloride 0.04 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1683 and Pf6N2E2_1684 are separated by 147 nucleotidesPf6N2E2_1684 and Pf6N2E2_1685 are separated by 64 nucleotides Pf6N2E2_1683 - Fe2+-dicitrate sensor, membrane component, at 1,990,742 to 1,991,719 _1683 Pf6N2E2_1684 - Ferrichrome-iron receptor, at 1,991,867 to 1,994,230 _1684 Pf6N2E2_1685 - Vitamin B12 ABC transporter, B12-binding component BtuF, at 1,994,295 to 1,995,482 _1685 Position (kb) 1991 1992 1993 1994 1995Strain fitness (log2 ratio) -2 -1 0 1 2at 1991.170 kb on - strand, within Pf6N2E2_1683at 1991.349 kb on + strand, within Pf6N2E2_1683at 1991.733 kb on - strandat 1992.090 kb on - strandat 1992.118 kb on - strand, within Pf6N2E2_1684at 1992.165 kb on - strand, within Pf6N2E2_1684at 1992.244 kb on + strand, within Pf6N2E2_1684at 1992.335 kb on + strand, within Pf6N2E2_1684at 1992.335 kb on + strand, within Pf6N2E2_1684at 1992.336 kb on - strand, within Pf6N2E2_1684at 1992.336 kb on - strand, within Pf6N2E2_1684at 1992.507 kb on - strand, within Pf6N2E2_1684at 1992.507 kb on - strand, within Pf6N2E2_1684at 1992.543 kb on - strand, within Pf6N2E2_1684at 1992.588 kb on - strand, within Pf6N2E2_1684at 1992.840 kb on - strand, within Pf6N2E2_1684at 1992.840 kb on - strand, within Pf6N2E2_1684at 1993.158 kb on - strand, within Pf6N2E2_1684at 1993.158 kb on - strand, within Pf6N2E2_1684at 1993.265 kb on - strand, within Pf6N2E2_1684at 1993.314 kb on - strand, within Pf6N2E2_1684at 1993.314 kb on - strand, within Pf6N2E2_1684at 1993.314 kb on - strand, within Pf6N2E2_1684at 1993.478 kb on + strand, within Pf6N2E2_1684at 1993.478 kb on + strand, within Pf6N2E2_1684at 1993.479 kb on - strand, within Pf6N2E2_1684at 1993.479 kb on - strand, within Pf6N2E2_1684at 1993.506 kb on - strand, within Pf6N2E2_1684at 1993.572 kb on - strand, within Pf6N2E2_1684at 1993.574 kb on + strand, within Pf6N2E2_1684at 1993.574 kb on + strand, within Pf6N2E2_1684at 1993.575 kb on - strand, within Pf6N2E2_1684at 1993.575 kb on - strand, within Pf6N2E2_1684at 1993.575 kb on - strand, within Pf6N2E2_1684at 1993.580 kb on + strand, within Pf6N2E2_1684at 1993.580 kb on + strand, within Pf6N2E2_1684at 1993.652 kb on + strand, within Pf6N2E2_1684at 1993.652 kb on + strand, within Pf6N2E2_1684at 1993.652 kb on + strand, within Pf6N2E2_1684at 1993.653 kb on - strand, within Pf6N2E2_1684at 1993.692 kb on - strand, within Pf6N2E2_1684at 1993.692 kb on - strand, within Pf6N2E2_1684at 1993.857 kb on + strand, within Pf6N2E2_1684at 1993.907 kb on - strand, within Pf6N2E2_1684at 1994.168 kb on + strandat 1994.169 kb on - strandat 1994.585 kb on + strand, within Pf6N2E2_1685at 1994.590 kb on - strand, within Pf6N2E2_1685at 1994.745 kb on + strand, within Pf6N2E2_1685at 1994.784 kb on + strand, within Pf6N2E2_1685at 1994.785 kb on - strand, within Pf6N2E2_1685at 1994.785 kb on - strand, within Pf6N2E2_1685at 1994.976 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM
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1,991,170 - Pf6N2E2_1683 0.44 +1.3
1,991,349 + Pf6N2E2_1683 0.62 +0.3
1,991,733 - -1.0
1,992,090 - -0.1
1,992,118 - Pf6N2E2_1684 0.11 -1.0
1,992,165 - Pf6N2E2_1684 0.13 +1.3
1,992,244 + Pf6N2E2_1684 0.16 +2.1
1,992,335 + Pf6N2E2_1684 0.20 +1.2
1,992,335 + Pf6N2E2_1684 0.20 +2.2
1,992,336 - Pf6N2E2_1684 0.20 +1.4
1,992,336 - Pf6N2E2_1684 0.20 -0.8
1,992,507 - Pf6N2E2_1684 0.27 +0.2
1,992,507 - Pf6N2E2_1684 0.27 +0.6
1,992,543 - Pf6N2E2_1684 0.29 -0.4
1,992,588 - Pf6N2E2_1684 0.30 -0.8
1,992,840 - Pf6N2E2_1684 0.41 -1.4
1,992,840 - Pf6N2E2_1684 0.41 +0.8
1,993,158 - Pf6N2E2_1684 0.55 -1.8
1,993,158 - Pf6N2E2_1684 0.55 +0.2
1,993,265 - Pf6N2E2_1684 0.59 +1.1
1,993,314 - Pf6N2E2_1684 0.61 -1.0
1,993,314 - Pf6N2E2_1684 0.61 +0.0
1,993,314 - Pf6N2E2_1684 0.61 -0.4
1,993,478 + Pf6N2E2_1684 0.68 +0.1
1,993,478 + Pf6N2E2_1684 0.68 -0.9
1,993,479 - Pf6N2E2_1684 0.68 -1.8
1,993,479 - Pf6N2E2_1684 0.68 +0.7
1,993,506 - Pf6N2E2_1684 0.69 +0.8
1,993,572 - Pf6N2E2_1684 0.72 +1.1
1,993,574 + Pf6N2E2_1684 0.72 -0.4
1,993,574 + Pf6N2E2_1684 0.72 +1.1
1,993,575 - Pf6N2E2_1684 0.72 +0.4
1,993,575 - Pf6N2E2_1684 0.72 +0.5
1,993,575 - Pf6N2E2_1684 0.72 +0.6
1,993,580 + Pf6N2E2_1684 0.72 +0.6
1,993,580 + Pf6N2E2_1684 0.72 -1.0
1,993,652 + Pf6N2E2_1684 0.76 -0.4
1,993,652 + Pf6N2E2_1684 0.76 +1.3
1,993,652 + Pf6N2E2_1684 0.76 +1.5
1,993,653 - Pf6N2E2_1684 0.76 -0.4
1,993,692 - Pf6N2E2_1684 0.77 +0.2
1,993,692 - Pf6N2E2_1684 0.77 -0.6
1,993,857 + Pf6N2E2_1684 0.84 -0.9
1,993,907 - Pf6N2E2_1684 0.86 +2.6
1,994,168 + +1.7
1,994,169 - -1.7
1,994,585 + Pf6N2E2_1685 0.24 +0.6
1,994,590 - Pf6N2E2_1685 0.25 +2.1
1,994,745 + Pf6N2E2_1685 0.38 -1.4
1,994,784 + Pf6N2E2_1685 0.41 +0.8
1,994,785 - Pf6N2E2_1685 0.41 -0.0
1,994,785 - Pf6N2E2_1685 0.41 -0.0
1,994,976 + +0.6

Or see this region's nucleotide sequence