Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1678 and Pf6N2E2_1679 are separated by 143 nucleotides Pf6N2E2_1679 and Pf6N2E2_1680 are separated by 55 nucleotides Pf6N2E2_1680 and Pf6N2E2_1681 are separated by 245 nucleotides
Pf6N2E2_1678 - Major facilitator family transporter, at 1,984,197 to 1,985,468
_1678
Pf6N2E2_1679 - SUGAR TRANSPORTER, at 1,985,612 to 1,986,391
_1679
Pf6N2E2_1680 - Ferrichrome-iron receptor, at 1,986,447 to 1,988,669
_1680
Pf6N2E2_1681 - Multidrug resistance protein B, at 1,988,915 to 1,990,141
_1681
Position (kb)
1986
1987
1988
1989 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1985.462 kb on + strand at 1985.463 kb on - strand at 1985.463 kb on - strand at 1985.466 kb on + strand at 1985.466 kb on + strand at 1985.466 kb on + strand at 1985.466 kb on + strand at 1985.466 kb on + strand at 1985.467 kb on - strand at 1985.467 kb on - strand at 1985.467 kb on - strand at 1985.477 kb on + strand at 1985.477 kb on + strand at 1985.477 kb on + strand at 1985.477 kb on + strand at 1985.478 kb on - strand at 1985.478 kb on - strand at 1985.478 kb on - strand at 1985.478 kb on - strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.481 kb on + strand at 1985.482 kb on - strand at 1985.482 kb on - strand at 1985.524 kb on - strand at 1985.627 kb on + strand at 1985.627 kb on + strand at 1985.627 kb on + strand at 1985.627 kb on + strand at 1985.628 kb on - strand at 1985.768 kb on + strand, within Pf6N2E2_1679 at 1985.769 kb on - strand, within Pf6N2E2_1679 at 1985.771 kb on + strand, within Pf6N2E2_1679 at 1985.771 kb on + strand, within Pf6N2E2_1679 at 1985.771 kb on + strand, within Pf6N2E2_1679 at 1985.771 kb on + strand, within Pf6N2E2_1679 at 1985.826 kb on - strand, within Pf6N2E2_1679 at 1985.826 kb on - strand, within Pf6N2E2_1679 at 1985.871 kb on - strand, within Pf6N2E2_1679 at 1985.940 kb on + strand, within Pf6N2E2_1679 at 1985.940 kb on + strand, within Pf6N2E2_1679 at 1985.941 kb on - strand, within Pf6N2E2_1679 at 1986.054 kb on + strand, within Pf6N2E2_1679 at 1986.054 kb on + strand, within Pf6N2E2_1679 at 1986.054 kb on + strand, within Pf6N2E2_1679 at 1986.055 kb on - strand, within Pf6N2E2_1679 at 1986.185 kb on + strand, within Pf6N2E2_1679 at 1986.185 kb on + strand, within Pf6N2E2_1679 at 1986.185 kb on + strand, within Pf6N2E2_1679 at 1986.186 kb on - strand, within Pf6N2E2_1679 at 1986.186 kb on - strand, within Pf6N2E2_1679 at 1986.509 kb on - strand at 1986.661 kb on + strand at 1986.662 kb on - strand at 1986.676 kb on + strand, within Pf6N2E2_1680 at 1986.770 kb on + strand, within Pf6N2E2_1680 at 1986.771 kb on - strand, within Pf6N2E2_1680 at 1986.771 kb on - strand, within Pf6N2E2_1680 at 1986.994 kb on + strand, within Pf6N2E2_1680 at 1986.995 kb on - strand, within Pf6N2E2_1680 at 1986.995 kb on - strand, within Pf6N2E2_1680 at 1987.027 kb on + strand, within Pf6N2E2_1680 at 1987.027 kb on + strand, within Pf6N2E2_1680 at 1987.028 kb on - strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.099 kb on + strand, within Pf6N2E2_1680 at 1987.100 kb on - strand, within Pf6N2E2_1680 at 1987.100 kb on - strand, within Pf6N2E2_1680 at 1987.100 kb on - strand, within Pf6N2E2_1680 at 1987.100 kb on - strand, within Pf6N2E2_1680 at 1987.105 kb on + strand, within Pf6N2E2_1680 at 1987.105 kb on + strand, within Pf6N2E2_1680 at 1987.106 kb on - strand, within Pf6N2E2_1680 at 1987.106 kb on - strand, within Pf6N2E2_1680 at 1987.106 kb on - strand, within Pf6N2E2_1680 at 1987.106 kb on - strand, within Pf6N2E2_1680 at 1987.106 kb on - strand, within Pf6N2E2_1680 at 1987.109 kb on - strand, within Pf6N2E2_1680 at 1987.109 kb on - strand, within Pf6N2E2_1680 at 1987.204 kb on + strand, within Pf6N2E2_1680 at 1987.204 kb on + strand, within Pf6N2E2_1680 at 1987.205 kb on - strand, within Pf6N2E2_1680 at 1987.369 kb on + strand, within Pf6N2E2_1680 at 1987.369 kb on + strand, within Pf6N2E2_1680 at 1987.370 kb on - strand, within Pf6N2E2_1680 at 1987.370 kb on - strand, within Pf6N2E2_1680 at 1987.370 kb on - strand, within Pf6N2E2_1680 at 1987.386 kb on - strand, within Pf6N2E2_1680 at 1987.427 kb on - strand, within Pf6N2E2_1680 at 1987.559 kb on + strand, within Pf6N2E2_1680 at 1987.559 kb on + strand, within Pf6N2E2_1680 at 1987.560 kb on - strand, within Pf6N2E2_1680 at 1987.560 kb on - strand, within Pf6N2E2_1680 at 1987.560 kb on - strand, within Pf6N2E2_1680 at 1987.560 kb on - strand, within Pf6N2E2_1680 at 1987.568 kb on + strand, within Pf6N2E2_1680 at 1987.568 kb on + strand, within Pf6N2E2_1680 at 1987.568 kb on + strand, within Pf6N2E2_1680 at 1987.569 kb on - strand, within Pf6N2E2_1680 at 1987.569 kb on - strand, within Pf6N2E2_1680 at 1987.857 kb on - strand, within Pf6N2E2_1680 at 1987.857 kb on - strand, within Pf6N2E2_1680 at 1988.002 kb on + strand, within Pf6N2E2_1680 at 1988.003 kb on - strand, within Pf6N2E2_1680 at 1988.095 kb on + strand, within Pf6N2E2_1680 at 1988.095 kb on + strand, within Pf6N2E2_1680 at 1988.095 kb on + strand, within Pf6N2E2_1680 at 1988.096 kb on - strand, within Pf6N2E2_1680 at 1988.159 kb on - strand, within Pf6N2E2_1680 at 1988.347 kb on + strand, within Pf6N2E2_1680 at 1988.348 kb on - strand, within Pf6N2E2_1680 at 1988.348 kb on - strand, within Pf6N2E2_1680 at 1988.675 kb on - strand at 1988.675 kb on - strand at 1988.738 kb on + strand at 1988.738 kb on + strand at 1988.739 kb on - strand at 1988.963 kb on + strand at 1988.964 kb on - strand at 1988.964 kb on - strand at 1989.160 kb on + strand, within Pf6N2E2_1681 at 1989.161 kb on - strand, within Pf6N2E2_1681 at 1989.161 kb on - strand, within Pf6N2E2_1681 at 1989.161 kb on - strand, within Pf6N2E2_1681 at 1989.161 kb on - strand, within Pf6N2E2_1681 at 1989.279 kb on - strand, within Pf6N2E2_1681 at 1989.390 kb on + strand, within Pf6N2E2_1681 at 1989.391 kb on - strand, within Pf6N2E2_1681 at 1989.413 kb on + strand, within Pf6N2E2_1681 at 1989.413 kb on + strand, within Pf6N2E2_1681 at 1989.414 kb on - strand, within Pf6N2E2_1681 at 1989.414 kb on - strand, within Pf6N2E2_1681 at 1989.559 kb on + strand, within Pf6N2E2_1681 at 1989.559 kb on + strand, within Pf6N2E2_1681 at 1989.559 kb on + strand, within Pf6N2E2_1681 at 1989.559 kb on + strand, within Pf6N2E2_1681 at 1989.559 kb on + strand, within Pf6N2E2_1681 at 1989.560 kb on - strand, within Pf6N2E2_1681 at 1989.650 kb on + strand, within Pf6N2E2_1681 at 1989.650 kb on + strand, within Pf6N2E2_1681 at 1989.650 kb on + strand, within Pf6N2E2_1681 at 1989.650 kb on + strand, within Pf6N2E2_1681 at 1989.651 kb on - strand, within Pf6N2E2_1681 at 1989.651 kb on - strand, within Pf6N2E2_1681
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,985,462 + +0.6 1,985,463 - -1.0 1,985,463 - -2.2 1,985,466 + +0.9 1,985,466 + +0.4 1,985,466 + -0.4 1,985,466 + +0.4 1,985,466 + -1.0 1,985,467 - +0.9 1,985,467 - +0.0 1,985,467 - -0.6 1,985,477 + +0.9 1,985,477 + +2.9 1,985,477 + -1.4 1,985,477 + -1.2 1,985,478 - -0.6 1,985,478 - -2.0 1,985,478 - +1.2 1,985,478 - +0.2 1,985,481 + +1.1 1,985,481 + +0.0 1,985,481 + -2.0 1,985,481 + +0.6 1,985,481 + +0.8 1,985,481 + +0.6 1,985,481 + +2.3 1,985,481 + +1.4 1,985,481 + +0.6 1,985,481 + +0.1 1,985,481 + +1.0 1,985,481 + -0.8 1,985,481 + +0.2 1,985,481 + -1.4 1,985,482 - -1.0 1,985,482 - +0.3 1,985,524 - -0.4 1,985,627 + +1.5 1,985,627 + +1.8 1,985,627 + +2.0 1,985,627 + +0.4 1,985,628 - -2.0 1,985,768 + Pf6N2E2_1679 0.20 +0.6 1,985,769 - Pf6N2E2_1679 0.20 +0.7 1,985,771 + Pf6N2E2_1679 0.20 +0.6 1,985,771 + Pf6N2E2_1679 0.20 -0.4 1,985,771 + Pf6N2E2_1679 0.20 +0.7 1,985,771 + Pf6N2E2_1679 0.20 +1.9 1,985,826 - Pf6N2E2_1679 0.27 -0.2 1,985,826 - Pf6N2E2_1679 0.27 +0.1 1,985,871 - Pf6N2E2_1679 0.33 +1.3 1,985,940 + Pf6N2E2_1679 0.42 -0.4 1,985,940 + Pf6N2E2_1679 0.42 +0.6 1,985,941 - Pf6N2E2_1679 0.42 +2.9 1,986,054 + Pf6N2E2_1679 0.57 +0.6 1,986,054 + Pf6N2E2_1679 0.57 -0.2 1,986,054 + Pf6N2E2_1679 0.57 +0.6 1,986,055 - Pf6N2E2_1679 0.57 +0.6 1,986,185 + Pf6N2E2_1679 0.73 +1.2 1,986,185 + Pf6N2E2_1679 0.73 -0.4 1,986,185 + Pf6N2E2_1679 0.73 +0.8 1,986,186 - Pf6N2E2_1679 0.74 -1.4 1,986,186 - Pf6N2E2_1679 0.74 -2.8 1,986,509 - +0.9 1,986,661 + +0.6 1,986,662 - -0.9 1,986,676 + Pf6N2E2_1680 0.10 -1.0 1,986,770 + Pf6N2E2_1680 0.15 +1.1 1,986,771 - Pf6N2E2_1680 0.15 -0.7 1,986,771 - Pf6N2E2_1680 0.15 -0.2 1,986,994 + Pf6N2E2_1680 0.25 -1.8 1,986,995 - Pf6N2E2_1680 0.25 +0.1 1,986,995 - Pf6N2E2_1680 0.25 -1.7 1,987,027 + Pf6N2E2_1680 0.26 +0.6 1,987,027 + Pf6N2E2_1680 0.26 -0.2 1,987,028 - Pf6N2E2_1680 0.26 +1.5 1,987,099 + Pf6N2E2_1680 0.29 +1.8 1,987,099 + Pf6N2E2_1680 0.29 -0.6 1,987,099 + Pf6N2E2_1680 0.29 -0.4 1,987,099 + Pf6N2E2_1680 0.29 -0.4 1,987,099 + Pf6N2E2_1680 0.29 -0.4 1,987,099 + Pf6N2E2_1680 0.29 -2.4 1,987,099 + Pf6N2E2_1680 0.29 +1.5 1,987,100 - Pf6N2E2_1680 0.29 +1.0 1,987,100 - Pf6N2E2_1680 0.29 +1.7 1,987,100 - Pf6N2E2_1680 0.29 +0.6 1,987,100 - Pf6N2E2_1680 0.29 -2.8 1,987,105 + Pf6N2E2_1680 0.30 -0.4 1,987,105 + Pf6N2E2_1680 0.30 -0.4 1,987,106 - Pf6N2E2_1680 0.30 +0.6 1,987,106 - Pf6N2E2_1680 0.30 -2.0 1,987,106 - Pf6N2E2_1680 0.30 -0.4 1,987,106 - Pf6N2E2_1680 0.30 +2.1 1,987,106 - Pf6N2E2_1680 0.30 -0.4 1,987,109 - Pf6N2E2_1680 0.30 -1.8 1,987,109 - Pf6N2E2_1680 0.30 +1.5 1,987,204 + Pf6N2E2_1680 0.34 -1.0 1,987,204 + Pf6N2E2_1680 0.34 -0.9 1,987,205 - Pf6N2E2_1680 0.34 +0.2 1,987,369 + Pf6N2E2_1680 0.41 +1.9 1,987,369 + Pf6N2E2_1680 0.41 -1.3 1,987,370 - Pf6N2E2_1680 0.42 +0.6 1,987,370 - Pf6N2E2_1680 0.42 +1.0 1,987,370 - Pf6N2E2_1680 0.42 +1.0 1,987,386 - Pf6N2E2_1680 0.42 -2.4 1,987,427 - Pf6N2E2_1680 0.44 -2.2 1,987,559 + Pf6N2E2_1680 0.50 -1.8 1,987,559 + Pf6N2E2_1680 0.50 +0.2 1,987,560 - Pf6N2E2_1680 0.50 -1.0 1,987,560 - Pf6N2E2_1680 0.50 -0.4 1,987,560 - Pf6N2E2_1680 0.50 -0.1 1,987,560 - Pf6N2E2_1680 0.50 +1.6 1,987,568 + Pf6N2E2_1680 0.50 -0.0 1,987,568 + Pf6N2E2_1680 0.50 -0.4 1,987,568 + Pf6N2E2_1680 0.50 +0.6 1,987,569 - Pf6N2E2_1680 0.50 -1.4 1,987,569 - Pf6N2E2_1680 0.50 +0.8 1,987,857 - Pf6N2E2_1680 0.63 +0.6 1,987,857 - Pf6N2E2_1680 0.63 +0.4 1,988,002 + Pf6N2E2_1680 0.70 +1.1 1,988,003 - Pf6N2E2_1680 0.70 -1.0 1,988,095 + Pf6N2E2_1680 0.74 -0.3 1,988,095 + Pf6N2E2_1680 0.74 -2.4 1,988,095 + Pf6N2E2_1680 0.74 +0.2 1,988,096 - Pf6N2E2_1680 0.74 +0.6 1,988,159 - Pf6N2E2_1680 0.77 -1.0 1,988,347 + Pf6N2E2_1680 0.85 -0.4 1,988,348 - Pf6N2E2_1680 0.86 +0.1 1,988,348 - Pf6N2E2_1680 0.86 +2.6 1,988,675 - +0.6 1,988,675 - +0.3 1,988,738 + +0.6 1,988,738 + -2.1 1,988,739 - -0.5 1,988,963 + -1.8 1,988,964 - +1.2 1,988,964 - +0.3 1,989,160 + Pf6N2E2_1681 0.20 -1.2 1,989,161 - Pf6N2E2_1681 0.20 +0.4 1,989,161 - Pf6N2E2_1681 0.20 -1.9 1,989,161 - Pf6N2E2_1681 0.20 +1.2 1,989,161 - Pf6N2E2_1681 0.20 -1.0 1,989,279 - Pf6N2E2_1681 0.30 -0.1 1,989,390 + Pf6N2E2_1681 0.39 -0.2 1,989,391 - Pf6N2E2_1681 0.39 +0.9 1,989,413 + Pf6N2E2_1681 0.41 -0.4 1,989,413 + Pf6N2E2_1681 0.41 -1.0 1,989,414 - Pf6N2E2_1681 0.41 -2.2 1,989,414 - Pf6N2E2_1681 0.41 -2.2 1,989,559 + Pf6N2E2_1681 0.52 -0.0 1,989,559 + Pf6N2E2_1681 0.52 -0.4 1,989,559 + Pf6N2E2_1681 0.52 -1.1 1,989,559 + Pf6N2E2_1681 0.52 +1.6 1,989,559 + Pf6N2E2_1681 0.52 -0.2 1,989,560 - Pf6N2E2_1681 0.53 -1.0 1,989,650 + Pf6N2E2_1681 0.60 -1.1 1,989,650 + Pf6N2E2_1681 0.60 +0.6 1,989,650 + Pf6N2E2_1681 0.60 +0.4 1,989,650 + Pf6N2E2_1681 0.60 +1.5 1,989,651 - Pf6N2E2_1681 0.60 -2.9 1,989,651 - Pf6N2E2_1681 0.60 -0.8
Or see this region's nucleotide sequence