Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1516 and Pf6N2E2_1517 are separated by 130 nucleotides Pf6N2E2_1517 and Pf6N2E2_1518 are separated by 88 nucleotides Pf6N2E2_1518 and Pf6N2E2_1519 are separated by 331 nucleotides
Pf6N2E2_1516 - transcriptional regulator, Crp/Fnr family, at 1,771,810 to 1,772,562
_1516
Pf6N2E2_1517 - Arginine/ornithine antiporter ArcD, at 1,772,693 to 1,774,186
_1517
Pf6N2E2_1518 - heat shock protein, HSP20 family, at 1,774,275 to 1,774,814
_1518
Pf6N2E2_1519 - Metallo-beta-lactamase family protein, RNA-specific, at 1,775,146 to 1,776,501
_1519
Position (kb) 1772
1773
1774
1775 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1771.778 kb on + strand at 1771.779 kb on - strand at 1771.779 kb on - strand at 1771.779 kb on - strand at 1771.793 kb on + strand at 1771.794 kb on - strand at 1772.009 kb on - strand, within Pf6N2E2_1516 at 1772.009 kb on - strand, within Pf6N2E2_1516 at 1772.052 kb on - strand, within Pf6N2E2_1516 at 1772.211 kb on - strand, within Pf6N2E2_1516 at 1772.211 kb on - strand, within Pf6N2E2_1516 at 1772.325 kb on - strand, within Pf6N2E2_1516 at 1772.347 kb on - strand, within Pf6N2E2_1516 at 1772.360 kb on - strand, within Pf6N2E2_1516 at 1772.588 kb on + strand at 1772.713 kb on + strand at 1772.714 kb on - strand at 1773.077 kb on + strand, within Pf6N2E2_1517 at 1773.078 kb on - strand, within Pf6N2E2_1517 at 1773.108 kb on - strand, within Pf6N2E2_1517 at 1773.108 kb on - strand, within Pf6N2E2_1517 at 1773.133 kb on + strand, within Pf6N2E2_1517 at 1773.133 kb on + strand, within Pf6N2E2_1517 at 1773.136 kb on + strand, within Pf6N2E2_1517 at 1773.137 kb on - strand, within Pf6N2E2_1517 at 1773.414 kb on + strand, within Pf6N2E2_1517 at 1773.415 kb on - strand, within Pf6N2E2_1517 at 1773.415 kb on - strand, within Pf6N2E2_1517 at 1773.415 kb on - strand, within Pf6N2E2_1517 at 1773.415 kb on - strand, within Pf6N2E2_1517 at 1773.821 kb on + strand, within Pf6N2E2_1517 at 1773.866 kb on + strand, within Pf6N2E2_1517 at 1773.866 kb on + strand, within Pf6N2E2_1517 at 1773.884 kb on + strand, within Pf6N2E2_1517 at 1773.884 kb on + strand, within Pf6N2E2_1517 at 1773.884 kb on + strand, within Pf6N2E2_1517 at 1773.918 kb on - strand, within Pf6N2E2_1517 at 1773.996 kb on - strand, within Pf6N2E2_1517 at 1774.014 kb on - strand, within Pf6N2E2_1517 at 1774.038 kb on - strand at 1774.185 kb on - strand at 1774.662 kb on + strand, within Pf6N2E2_1518 at 1774.873 kb on + strand at 1774.874 kb on - strand at 1775.039 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,771,778 + -0.4 1,771,779 - -1.6 1,771,779 - -0.9 1,771,779 - +1.0 1,771,793 + -0.6 1,771,794 - -0.7 1,772,009 - Pf6N2E2_1516 0.26 +0.0 1,772,009 - Pf6N2E2_1516 0.26 -1.6 1,772,052 - Pf6N2E2_1516 0.32 +1.3 1,772,211 - Pf6N2E2_1516 0.53 +1.3 1,772,211 - Pf6N2E2_1516 0.53 -0.4 1,772,325 - Pf6N2E2_1516 0.68 -2.4 1,772,347 - Pf6N2E2_1516 0.71 +0.8 1,772,360 - Pf6N2E2_1516 0.73 -0.2 1,772,588 + +0.6 1,772,713 + +2.5 1,772,714 - +0.1 1,773,077 + Pf6N2E2_1517 0.26 -1.6 1,773,078 - Pf6N2E2_1517 0.26 -0.1 1,773,108 - Pf6N2E2_1517 0.28 +0.0 1,773,108 - Pf6N2E2_1517 0.28 +1.0 1,773,133 + Pf6N2E2_1517 0.29 +2.2 1,773,133 + Pf6N2E2_1517 0.29 -0.6 1,773,136 + Pf6N2E2_1517 0.30 +1.3 1,773,137 - Pf6N2E2_1517 0.30 +1.3 1,773,414 + Pf6N2E2_1517 0.48 -2.4 1,773,415 - Pf6N2E2_1517 0.48 -1.1 1,773,415 - Pf6N2E2_1517 0.48 +0.1 1,773,415 - Pf6N2E2_1517 0.48 -1.4 1,773,415 - Pf6N2E2_1517 0.48 -1.1 1,773,821 + Pf6N2E2_1517 0.76 -1.6 1,773,866 + Pf6N2E2_1517 0.79 -0.6 1,773,866 + Pf6N2E2_1517 0.79 +0.3 1,773,884 + Pf6N2E2_1517 0.80 +0.0 1,773,884 + Pf6N2E2_1517 0.80 -0.4 1,773,884 + Pf6N2E2_1517 0.80 -1.5 1,773,918 - Pf6N2E2_1517 0.82 +0.4 1,773,996 - Pf6N2E2_1517 0.87 -0.6 1,774,014 - Pf6N2E2_1517 0.88 -1.4 1,774,038 - -0.5 1,774,185 - -0.3 1,774,662 + Pf6N2E2_1518 0.72 -0.2 1,774,873 + -1.0 1,774,874 - +0.3 1,775,039 - +0.4
Or see this region's nucleotide sequence