Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1424 and Pf6N2E2_1425 are separated by 48 nucleotides Pf6N2E2_1425 and Pf6N2E2_1426 are separated by 39 nucleotides
Pf6N2E2_1424 - transcriptional regulator, TetR family, at 1,672,418 to 1,673,029
_1424
Pf6N2E2_1425 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 1,673,078 to 1,674,181
_1425
Pf6N2E2_1426 - FIG00966546: hypothetical protein, at 1,674,221 to 1,675,558
_1426
Position (kb)
1673
1674
1675 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1672.151 kb on + strand at 1672.170 kb on - strand at 1672.170 kb on - strand at 1672.307 kb on + strand at 1672.389 kb on + strand at 1672.390 kb on - strand at 1672.390 kb on - strand at 1672.569 kb on - strand, within Pf6N2E2_1424 at 1672.686 kb on - strand, within Pf6N2E2_1424 at 1672.994 kb on - strand at 1673.147 kb on + strand at 1673.148 kb on - strand at 1673.182 kb on + strand at 1673.182 kb on + strand at 1673.182 kb on + strand at 1673.183 kb on - strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.184 kb on + strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.185 kb on - strand at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.298 kb on + strand, within Pf6N2E2_1425 at 1673.299 kb on - strand, within Pf6N2E2_1425 at 1673.299 kb on - strand, within Pf6N2E2_1425 at 1673.313 kb on + strand, within Pf6N2E2_1425 at 1673.523 kb on + strand, within Pf6N2E2_1425 at 1673.524 kb on - strand, within Pf6N2E2_1425 at 1673.684 kb on - strand, within Pf6N2E2_1425 at 1673.713 kb on + strand, within Pf6N2E2_1425 at 1673.714 kb on - strand, within Pf6N2E2_1425 at 1673.714 kb on - strand, within Pf6N2E2_1425 at 1673.761 kb on + strand, within Pf6N2E2_1425 at 1673.763 kb on + strand, within Pf6N2E2_1425 at 1673.763 kb on + strand, within Pf6N2E2_1425 at 1674.085 kb on + strand at 1674.088 kb on - strand at 1674.222 kb on + strand at 1674.223 kb on - strand at 1674.223 kb on - strand at 1674.223 kb on - strand at 1674.231 kb on + strand at 1674.240 kb on + strand at 1674.314 kb on - strand at 1674.315 kb on + strand at 1674.315 kb on + strand at 1674.316 kb on - strand at 1674.366 kb on - strand, within Pf6N2E2_1426 at 1674.366 kb on - strand, within Pf6N2E2_1426 at 1674.366 kb on - strand, within Pf6N2E2_1426 at 1674.366 kb on - strand, within Pf6N2E2_1426 at 1674.366 kb on - strand, within Pf6N2E2_1426 at 1674.411 kb on + strand, within Pf6N2E2_1426 at 1674.438 kb on + strand, within Pf6N2E2_1426 at 1674.438 kb on + strand, within Pf6N2E2_1426 at 1674.439 kb on - strand, within Pf6N2E2_1426 at 1674.519 kb on - strand, within Pf6N2E2_1426 at 1674.525 kb on + strand, within Pf6N2E2_1426 at 1674.819 kb on + strand, within Pf6N2E2_1426 at 1674.819 kb on + strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.820 kb on - strand, within Pf6N2E2_1426 at 1674.924 kb on + strand, within Pf6N2E2_1426 at 1674.924 kb on + strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1674.925 kb on - strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.125 kb on + strand, within Pf6N2E2_1426 at 1675.126 kb on - strand, within Pf6N2E2_1426 at 1675.126 kb on - strand, within Pf6N2E2_1426 at 1675.176 kb on + strand, within Pf6N2E2_1426 at 1675.176 kb on + strand, within Pf6N2E2_1426 at 1675.177 kb on - strand, within Pf6N2E2_1426
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,672,151 + +1.1 1,672,170 - -0.4 1,672,170 - +1.8 1,672,307 + -0.1 1,672,389 + -0.5 1,672,390 - +0.9 1,672,390 - -1.2 1,672,569 - Pf6N2E2_1424 0.25 -2.5 1,672,686 - Pf6N2E2_1424 0.44 +0.3 1,672,994 - +3.4 1,673,147 + +0.4 1,673,148 - +0.2 1,673,182 + -3.8 1,673,182 + +0.7 1,673,182 + +0.5 1,673,183 - -0.3 1,673,184 + +1.8 1,673,184 + -2.2 1,673,184 + +1.5 1,673,184 + +0.4 1,673,184 + -0.4 1,673,184 + -1.2 1,673,184 + -2.6 1,673,184 + +0.2 1,673,184 + +0.0 1,673,184 + -3.2 1,673,184 + +0.4 1,673,185 - -0.8 1,673,185 - -0.6 1,673,185 - +0.7 1,673,185 - -0.2 1,673,185 - -1.6 1,673,185 - -0.5 1,673,185 - +0.4 1,673,185 - +1.0 1,673,185 - -1.2 1,673,185 - +0.6 1,673,185 - +0.2 1,673,185 - +0.7 1,673,298 + Pf6N2E2_1425 0.20 +1.2 1,673,298 + Pf6N2E2_1425 0.20 +1.1 1,673,298 + Pf6N2E2_1425 0.20 -1.2 1,673,299 - Pf6N2E2_1425 0.20 +0.6 1,673,299 - Pf6N2E2_1425 0.20 +1.2 1,673,313 + Pf6N2E2_1425 0.21 -1.2 1,673,523 + Pf6N2E2_1425 0.40 +0.7 1,673,524 - Pf6N2E2_1425 0.40 +1.0 1,673,684 - Pf6N2E2_1425 0.55 -2.6 1,673,713 + Pf6N2E2_1425 0.58 -0.4 1,673,714 - Pf6N2E2_1425 0.58 +0.4 1,673,714 - Pf6N2E2_1425 0.58 +1.4 1,673,761 + Pf6N2E2_1425 0.62 +0.4 1,673,763 + Pf6N2E2_1425 0.62 -0.6 1,673,763 + Pf6N2E2_1425 0.62 -1.2 1,674,085 + +0.5 1,674,088 - +0.4 1,674,222 + -0.4 1,674,223 - -0.0 1,674,223 - +1.0 1,674,223 - +1.0 1,674,231 + -1.2 1,674,240 + +0.7 1,674,314 - +0.4 1,674,315 + -1.2 1,674,315 + -1.4 1,674,316 - -0.8 1,674,366 - Pf6N2E2_1426 0.11 -1.2 1,674,366 - Pf6N2E2_1426 0.11 +0.2 1,674,366 - Pf6N2E2_1426 0.11 -0.6 1,674,366 - Pf6N2E2_1426 0.11 -1.6 1,674,366 - Pf6N2E2_1426 0.11 +0.4 1,674,411 + Pf6N2E2_1426 0.14 -0.5 1,674,438 + Pf6N2E2_1426 0.16 -0.8 1,674,438 + Pf6N2E2_1426 0.16 -1.7 1,674,439 - Pf6N2E2_1426 0.16 -0.6 1,674,519 - Pf6N2E2_1426 0.22 +0.4 1,674,525 + Pf6N2E2_1426 0.23 -0.3 1,674,819 + Pf6N2E2_1426 0.45 -2.1 1,674,819 + Pf6N2E2_1426 0.45 +0.4 1,674,820 - Pf6N2E2_1426 0.45 -1.5 1,674,820 - Pf6N2E2_1426 0.45 -1.6 1,674,820 - Pf6N2E2_1426 0.45 +1.3 1,674,924 + Pf6N2E2_1426 0.53 +1.0 1,674,924 + Pf6N2E2_1426 0.53 -2.0 1,674,925 - Pf6N2E2_1426 0.53 +0.0 1,674,925 - Pf6N2E2_1426 0.53 -1.2 1,674,925 - Pf6N2E2_1426 0.53 -1.2 1,675,125 + Pf6N2E2_1426 0.68 -2.0 1,675,125 + Pf6N2E2_1426 0.68 -1.2 1,675,125 + Pf6N2E2_1426 0.68 +0.6 1,675,126 - Pf6N2E2_1426 0.68 -3.3 1,675,126 - Pf6N2E2_1426 0.68 +0.2 1,675,176 + Pf6N2E2_1426 0.71 +0.7 1,675,176 + Pf6N2E2_1426 0.71 -1.3 1,675,177 - Pf6N2E2_1426 0.71 -3.5
Or see this region's nucleotide sequence