Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1336 and Pf6N2E2_1337 are separated by 237 nucleotides Pf6N2E2_1337 and Pf6N2E2_1338 are separated by 199 nucleotides
Pf6N2E2_1336 - AraC family transcription regulator, at 1,571,947 to 1,572,627
_1336
Pf6N2E2_1337 - Cys regulon transcriptional activator CysB, at 1,572,865 to 1,573,776
_1337
Pf6N2E2_1338 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 1,573,976 to 1,575,016
_1338
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1571.865 kb on - strand at 1571.865 kb on - strand at 1572.053 kb on - strand, within Pf6N2E2_1336 at 1572.111 kb on + strand, within Pf6N2E2_1336 at 1572.112 kb on - strand, within Pf6N2E2_1336 at 1572.237 kb on - strand, within Pf6N2E2_1336 at 1572.400 kb on - strand, within Pf6N2E2_1336 at 1572.658 kb on + strand at 1572.658 kb on + strand at 1572.658 kb on + strand at 1572.829 kb on + strand at 1572.829 kb on + strand at 1572.829 kb on + strand at 1572.829 kb on + strand at 1572.829 kb on + strand at 1572.830 kb on - strand at 1572.830 kb on - strand at 1572.830 kb on - strand at 1572.830 kb on - strand at 1572.830 kb on - strand at 1572.830 kb on - strand at 1572.834 kb on + strand at 1572.874 kb on + strand at 1572.875 kb on - strand at 1572.896 kb on - strand at 1572.896 kb on - strand at 1572.896 kb on - strand at 1572.949 kb on + strand at 1573.335 kb on + strand, within Pf6N2E2_1337 at 1573.335 kb on + strand, within Pf6N2E2_1337 at 1573.484 kb on - strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.624 kb on + strand, within Pf6N2E2_1337 at 1573.625 kb on - strand, within Pf6N2E2_1337 at 1573.713 kb on + strand at 1573.742 kb on - strand at 1573.742 kb on - strand at 1573.815 kb on + strand at 1573.815 kb on + strand at 1573.820 kb on - strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1573.971 kb on + strand at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.469 kb on + strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.470 kb on - strand, within Pf6N2E2_1338 at 1574.520 kb on + strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.521 kb on - strand, within Pf6N2E2_1338 at 1574.619 kb on + strand, within Pf6N2E2_1338 at 1574.620 kb on - strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.643 kb on + strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.644 kb on - strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.682 kb on + strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338 at 1574.683 kb on - strand, within Pf6N2E2_1338
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,571,865 - -2.0 1,571,865 - +2.7 1,572,053 - Pf6N2E2_1336 0.16 +0.8 1,572,111 + Pf6N2E2_1336 0.24 -1.0 1,572,112 - Pf6N2E2_1336 0.24 +0.6 1,572,237 - Pf6N2E2_1336 0.43 -0.6 1,572,400 - Pf6N2E2_1336 0.67 -2.8 1,572,658 + +0.7 1,572,658 + -1.6 1,572,658 + -1.2 1,572,829 + -0.6 1,572,829 + -1.0 1,572,829 + -1.2 1,572,829 + -0.1 1,572,829 + +0.4 1,572,830 - +0.4 1,572,830 - +1.4 1,572,830 - -0.5 1,572,830 - -2.0 1,572,830 - +0.2 1,572,830 - +2.2 1,572,834 + -0.6 1,572,874 + +0.2 1,572,875 - -0.6 1,572,896 - +0.6 1,572,896 - +0.3 1,572,896 - +1.2 1,572,949 + -2.4 1,573,335 + Pf6N2E2_1337 0.52 -1.2 1,573,335 + Pf6N2E2_1337 0.52 +4.2 1,573,484 - Pf6N2E2_1337 0.68 +0.4 1,573,624 + Pf6N2E2_1337 0.83 +1.4 1,573,624 + Pf6N2E2_1337 0.83 +0.9 1,573,624 + Pf6N2E2_1337 0.83 -0.9 1,573,624 + Pf6N2E2_1337 0.83 +1.2 1,573,625 - Pf6N2E2_1337 0.83 +1.7 1,573,713 + +1.4 1,573,742 - -0.1 1,573,742 - -0.8 1,573,815 + +2.0 1,573,815 + -0.0 1,573,820 - -0.3 1,573,971 + -1.4 1,573,971 + -3.2 1,573,971 + +0.5 1,573,971 + +1.2 1,574,469 + Pf6N2E2_1338 0.47 +0.4 1,574,469 + Pf6N2E2_1338 0.47 -1.5 1,574,470 - Pf6N2E2_1338 0.47 +1.8 1,574,470 - Pf6N2E2_1338 0.47 -1.6 1,574,470 - Pf6N2E2_1338 0.47 +0.4 1,574,470 - Pf6N2E2_1338 0.47 +0.6 1,574,520 + Pf6N2E2_1338 0.52 +1.5 1,574,521 - Pf6N2E2_1338 0.52 -0.3 1,574,521 - Pf6N2E2_1338 0.52 -1.4 1,574,619 + Pf6N2E2_1338 0.62 -2.0 1,574,620 - Pf6N2E2_1338 0.62 +1.4 1,574,643 + Pf6N2E2_1338 0.64 +1.4 1,574,643 + Pf6N2E2_1338 0.64 +0.4 1,574,643 + Pf6N2E2_1338 0.64 -0.3 1,574,643 + Pf6N2E2_1338 0.64 -2.0 1,574,644 - Pf6N2E2_1338 0.64 -0.8 1,574,644 - Pf6N2E2_1338 0.64 +1.7 1,574,682 + Pf6N2E2_1338 0.68 +0.3 1,574,682 + Pf6N2E2_1338 0.68 +1.3 1,574,683 - Pf6N2E2_1338 0.68 -0.1 1,574,683 - Pf6N2E2_1338 0.68 -1.3 1,574,683 - Pf6N2E2_1338 0.68 -0.2 1,574,683 - Pf6N2E2_1338 0.68 +2.2 1,574,683 - Pf6N2E2_1338 0.68 +0.4
Or see this region's nucleotide sequence