Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1087 and Pf6N2E2_1088 are separated by 159 nucleotides Pf6N2E2_1088 and Pf6N2E2_1089 are separated by 234 nucleotides
Pf6N2E2_1087 - Iron siderophore sensor protein, at 1,270,375 to 1,271,376
_1087
Pf6N2E2_1088 - hypothetical protein, at 1,271,536 to 1,276,368
_1088
Pf6N2E2_1089 - Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1), at 1,276,603 to 1,277,634
_1089
Position (kb)
1271
1272
1273
1274
1275
1276
1277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1270.691 kb on + strand, within Pf6N2E2_1087 at 1270.692 kb on - strand, within Pf6N2E2_1087 at 1270.692 kb on - strand, within Pf6N2E2_1087 at 1270.775 kb on - strand, within Pf6N2E2_1087 at 1270.799 kb on + strand, within Pf6N2E2_1087 at 1270.799 kb on + strand, within Pf6N2E2_1087 at 1270.800 kb on - strand, within Pf6N2E2_1087 at 1270.800 kb on - strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.808 kb on - strand, within Pf6N2E2_1087 at 1270.808 kb on - strand, within Pf6N2E2_1087 at 1270.938 kb on - strand, within Pf6N2E2_1087 at 1271.001 kb on + strand, within Pf6N2E2_1087 at 1271.001 kb on + strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.008 kb on + strand, within Pf6N2E2_1087 at 1271.036 kb on + strand, within Pf6N2E2_1087 at 1271.037 kb on - strand, within Pf6N2E2_1087 at 1271.073 kb on - strand, within Pf6N2E2_1087 at 1271.073 kb on - strand, within Pf6N2E2_1087 at 1271.095 kb on + strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087 at 1271.346 kb on - strand at 1271.489 kb on + strand at 1271.499 kb on + strand at 1271.499 kb on + strand at 1271.500 kb on - strand at 1271.500 kb on - strand at 1271.500 kb on - strand at 1271.626 kb on + strand at 1271.627 kb on - strand at 1271.627 kb on - strand at 1271.736 kb on - strand at 1271.762 kb on + strand at 1271.762 kb on + strand at 1271.762 kb on + strand at 1271.763 kb on - strand at 1271.763 kb on - strand at 1271.763 kb on - strand at 1271.873 kb on + strand at 1271.921 kb on + strand at 1271.921 kb on + strand at 1271.937 kb on + strand at 1271.938 kb on - strand at 1272.026 kb on + strand, within Pf6N2E2_1088 at 1272.026 kb on + strand, within Pf6N2E2_1088 at 1272.065 kb on + strand, within Pf6N2E2_1088 at 1272.065 kb on + strand, within Pf6N2E2_1088 at 1272.065 kb on + strand, within Pf6N2E2_1088 at 1272.065 kb on + strand, within Pf6N2E2_1088 at 1272.066 kb on - strand, within Pf6N2E2_1088 at 1272.094 kb on + strand, within Pf6N2E2_1088 at 1272.215 kb on - strand, within Pf6N2E2_1088 at 1272.225 kb on + strand, within Pf6N2E2_1088 at 1272.226 kb on - strand, within Pf6N2E2_1088 at 1272.226 kb on - strand, within Pf6N2E2_1088 at 1272.342 kb on - strand, within Pf6N2E2_1088 at 1272.461 kb on + strand, within Pf6N2E2_1088 at 1272.461 kb on + strand, within Pf6N2E2_1088 at 1272.461 kb on + strand, within Pf6N2E2_1088 at 1272.556 kb on + strand, within Pf6N2E2_1088 at 1272.640 kb on - strand at 1272.641 kb on + strand, within Pf6N2E2_1088 at 1272.641 kb on + strand, within Pf6N2E2_1088 at 1272.641 kb on + strand, within Pf6N2E2_1088 at 1272.641 kb on + strand, within Pf6N2E2_1088 at 1272.641 kb on + strand, within Pf6N2E2_1088 at 1272.642 kb on - strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.668 kb on + strand, within Pf6N2E2_1088 at 1272.835 kb on - strand, within Pf6N2E2_1088 at 1272.954 kb on - strand, within Pf6N2E2_1088 at 1273.258 kb on + strand, within Pf6N2E2_1088 at 1273.258 kb on + strand, within Pf6N2E2_1088 at 1273.340 kb on + strand, within Pf6N2E2_1088 at 1273.340 kb on + strand, within Pf6N2E2_1088 at 1273.385 kb on + strand, within Pf6N2E2_1088 at 1273.565 kb on + strand, within Pf6N2E2_1088 at 1273.677 kb on + strand, within Pf6N2E2_1088 at 1273.678 kb on - strand, within Pf6N2E2_1088 at 1273.678 kb on - strand, within Pf6N2E2_1088 at 1273.919 kb on + strand, within Pf6N2E2_1088 at 1273.919 kb on + strand, within Pf6N2E2_1088 at 1273.920 kb on - strand, within Pf6N2E2_1088 at 1273.920 kb on - strand, within Pf6N2E2_1088 at 1273.949 kb on + strand, within Pf6N2E2_1088 at 1273.949 kb on + strand, within Pf6N2E2_1088 at 1273.950 kb on - strand, within Pf6N2E2_1088 at 1273.977 kb on + strand, within Pf6N2E2_1088 at 1273.978 kb on - strand, within Pf6N2E2_1088 at 1274.189 kb on + strand, within Pf6N2E2_1088 at 1274.190 kb on - strand, within Pf6N2E2_1088 at 1274.190 kb on - strand, within Pf6N2E2_1088 at 1274.264 kb on + strand, within Pf6N2E2_1088 at 1274.264 kb on + strand, within Pf6N2E2_1088 at 1274.265 kb on - strand, within Pf6N2E2_1088 at 1274.265 kb on - strand, within Pf6N2E2_1088 at 1274.265 kb on - strand, within Pf6N2E2_1088 at 1274.265 kb on - strand, within Pf6N2E2_1088 at 1274.384 kb on - strand, within Pf6N2E2_1088 at 1274.470 kb on + strand, within Pf6N2E2_1088 at 1274.474 kb on - strand, within Pf6N2E2_1088 at 1274.540 kb on + strand, within Pf6N2E2_1088 at 1274.540 kb on + strand, within Pf6N2E2_1088 at 1274.656 kb on - strand, within Pf6N2E2_1088 at 1274.720 kb on - strand, within Pf6N2E2_1088 at 1274.808 kb on - strand, within Pf6N2E2_1088 at 1274.808 kb on - strand, within Pf6N2E2_1088 at 1274.923 kb on - strand, within Pf6N2E2_1088 at 1274.938 kb on + strand, within Pf6N2E2_1088 at 1274.938 kb on + strand, within Pf6N2E2_1088 at 1274.938 kb on + strand, within Pf6N2E2_1088 at 1274.939 kb on - strand, within Pf6N2E2_1088 at 1274.939 kb on - strand, within Pf6N2E2_1088 at 1274.939 kb on - strand, within Pf6N2E2_1088 at 1275.005 kb on + strand, within Pf6N2E2_1088 at 1275.006 kb on - strand, within Pf6N2E2_1088 at 1275.015 kb on + strand, within Pf6N2E2_1088 at 1275.143 kb on + strand, within Pf6N2E2_1088 at 1275.143 kb on + strand, within Pf6N2E2_1088 at 1275.214 kb on + strand, within Pf6N2E2_1088 at 1275.214 kb on + strand at 1275.242 kb on + strand, within Pf6N2E2_1088 at 1275.243 kb on - strand, within Pf6N2E2_1088 at 1275.243 kb on - strand, within Pf6N2E2_1088 at 1275.248 kb on + strand, within Pf6N2E2_1088 at 1275.248 kb on + strand, within Pf6N2E2_1088 at 1275.248 kb on + strand, within Pf6N2E2_1088 at 1275.249 kb on - strand, within Pf6N2E2_1088 at 1275.290 kb on - strand, within Pf6N2E2_1088 at 1275.483 kb on + strand, within Pf6N2E2_1088 at 1275.484 kb on - strand, within Pf6N2E2_1088 at 1275.484 kb on - strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.485 kb on + strand, within Pf6N2E2_1088 at 1275.486 kb on - strand, within Pf6N2E2_1088 at 1275.531 kb on + strand, within Pf6N2E2_1088 at 1275.665 kb on + strand, within Pf6N2E2_1088 at 1275.817 kb on + strand, within Pf6N2E2_1088 at 1275.817 kb on + strand, within Pf6N2E2_1088 at 1275.818 kb on - strand, within Pf6N2E2_1088 at 1276.330 kb on + strand at 1276.343 kb on - strand at 1276.454 kb on + strand at 1276.454 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.458 kb on + strand at 1276.459 kb on - strand at 1276.459 kb on - strand at 1276.459 kb on - strand at 1276.459 kb on - strand at 1276.459 kb on - strand at 1276.542 kb on + strand at 1276.543 kb on - strand at 1276.563 kb on + strand at 1276.563 kb on + strand at 1276.574 kb on + strand at 1276.575 kb on - strand at 1276.787 kb on - strand, within Pf6N2E2_1089 at 1276.787 kb on - strand, within Pf6N2E2_1089 at 1277.017 kb on + strand, within Pf6N2E2_1089
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,270,691 + Pf6N2E2_1087 0.32 -2.4 1,270,692 - Pf6N2E2_1087 0.32 -0.1 1,270,692 - Pf6N2E2_1087 0.32 -1.9 1,270,775 - Pf6N2E2_1087 0.40 +0.6 1,270,799 + Pf6N2E2_1087 0.42 -2.1 1,270,799 + Pf6N2E2_1087 0.42 +1.2 1,270,800 - Pf6N2E2_1087 0.42 +0.3 1,270,800 - Pf6N2E2_1087 0.42 -2.1 1,270,807 + Pf6N2E2_1087 0.43 -1.1 1,270,807 + Pf6N2E2_1087 0.43 -1.4 1,270,807 + Pf6N2E2_1087 0.43 -0.6 1,270,807 + Pf6N2E2_1087 0.43 -0.3 1,270,807 + Pf6N2E2_1087 0.43 +0.3 1,270,808 - Pf6N2E2_1087 0.43 -1.0 1,270,808 - Pf6N2E2_1087 0.43 -1.0 1,270,938 - Pf6N2E2_1087 0.56 +0.3 1,271,001 + Pf6N2E2_1087 0.62 -2.5 1,271,001 + Pf6N2E2_1087 0.62 +1.6 1,271,002 - Pf6N2E2_1087 0.63 -1.9 1,271,002 - Pf6N2E2_1087 0.63 -0.5 1,271,002 - Pf6N2E2_1087 0.63 -0.1 1,271,008 + Pf6N2E2_1087 0.63 -0.5 1,271,036 + Pf6N2E2_1087 0.66 -0.5 1,271,037 - Pf6N2E2_1087 0.66 -0.5 1,271,073 - Pf6N2E2_1087 0.70 +1.5 1,271,073 - Pf6N2E2_1087 0.70 -3.6 1,271,095 + Pf6N2E2_1087 0.72 +0.5 1,271,099 - Pf6N2E2_1087 0.72 -1.5 1,271,099 - Pf6N2E2_1087 0.72 +0.2 1,271,099 - Pf6N2E2_1087 0.72 -1.1 1,271,346 - +0.3 1,271,489 + -1.1 1,271,499 + +0.5 1,271,499 + +0.3 1,271,500 - -1.1 1,271,500 - -1.2 1,271,500 - -1.2 1,271,626 + -1.1 1,271,627 - +1.8 1,271,627 - +1.2 1,271,736 - +0.7 1,271,762 + -0.8 1,271,762 + +1.5 1,271,762 + +1.7 1,271,763 - -0.5 1,271,763 - +0.3 1,271,763 - -0.8 1,271,873 + -1.5 1,271,921 + +0.5 1,271,921 + -0.3 1,271,937 + +0.3 1,271,938 - +0.2 1,272,026 + Pf6N2E2_1088 0.10 +0.5 1,272,026 + Pf6N2E2_1088 0.10 +1.8 1,272,065 + Pf6N2E2_1088 0.11 +1.0 1,272,065 + Pf6N2E2_1088 0.11 -1.9 1,272,065 + Pf6N2E2_1088 0.11 +1.1 1,272,065 + Pf6N2E2_1088 0.11 +0.9 1,272,066 - Pf6N2E2_1088 0.11 -0.4 1,272,094 + Pf6N2E2_1088 0.12 +0.5 1,272,215 - Pf6N2E2_1088 0.14 -0.8 1,272,225 + Pf6N2E2_1088 0.14 -0.2 1,272,226 - Pf6N2E2_1088 0.14 +1.0 1,272,226 - Pf6N2E2_1088 0.14 -0.5 1,272,342 - Pf6N2E2_1088 0.17 -0.5 1,272,461 + Pf6N2E2_1088 0.19 -2.5 1,272,461 + Pf6N2E2_1088 0.19 -1.4 1,272,461 + Pf6N2E2_1088 0.19 -0.5 1,272,556 + Pf6N2E2_1088 0.21 +0.3 1,272,640 - -1.1 1,272,641 + Pf6N2E2_1088 0.23 -1.4 1,272,641 + Pf6N2E2_1088 0.23 -0.5 1,272,641 + Pf6N2E2_1088 0.23 -0.3 1,272,641 + Pf6N2E2_1088 0.23 -3.1 1,272,641 + Pf6N2E2_1088 0.23 -0.1 1,272,642 - Pf6N2E2_1088 0.23 -0.5 1,272,668 + Pf6N2E2_1088 0.23 +2.6 1,272,668 + Pf6N2E2_1088 0.23 +0.5 1,272,668 + Pf6N2E2_1088 0.23 +0.5 1,272,668 + Pf6N2E2_1088 0.23 -1.4 1,272,668 + Pf6N2E2_1088 0.23 -0.9 1,272,668 + Pf6N2E2_1088 0.23 +0.5 1,272,835 - Pf6N2E2_1088 0.27 +0.5 1,272,954 - Pf6N2E2_1088 0.29 +0.5 1,273,258 + Pf6N2E2_1088 0.36 -1.1 1,273,258 + Pf6N2E2_1088 0.36 +0.3 1,273,340 + Pf6N2E2_1088 0.37 -1.1 1,273,340 + Pf6N2E2_1088 0.37 -0.8 1,273,385 + Pf6N2E2_1088 0.38 -1.0 1,273,565 + Pf6N2E2_1088 0.42 +1.3 1,273,677 + Pf6N2E2_1088 0.44 +0.5 1,273,678 - Pf6N2E2_1088 0.44 -2.1 1,273,678 - Pf6N2E2_1088 0.44 -2.1 1,273,919 + Pf6N2E2_1088 0.49 -0.1 1,273,919 + Pf6N2E2_1088 0.49 +1.5 1,273,920 - Pf6N2E2_1088 0.49 +1.5 1,273,920 - Pf6N2E2_1088 0.49 -2.1 1,273,949 + Pf6N2E2_1088 0.50 -2.5 1,273,949 + Pf6N2E2_1088 0.50 +1.8 1,273,950 - Pf6N2E2_1088 0.50 +0.9 1,273,977 + Pf6N2E2_1088 0.51 +1.0 1,273,978 - Pf6N2E2_1088 0.51 -0.2 1,274,189 + Pf6N2E2_1088 0.55 -1.4 1,274,190 - Pf6N2E2_1088 0.55 -0.5 1,274,190 - Pf6N2E2_1088 0.55 -1.2 1,274,264 + Pf6N2E2_1088 0.56 -1.5 1,274,264 + Pf6N2E2_1088 0.56 -0.3 1,274,265 - Pf6N2E2_1088 0.56 -2.4 1,274,265 - Pf6N2E2_1088 0.56 -2.1 1,274,265 - Pf6N2E2_1088 0.56 -1.5 1,274,265 - Pf6N2E2_1088 0.56 -0.5 1,274,384 - Pf6N2E2_1088 0.59 +0.3 1,274,470 + Pf6N2E2_1088 0.61 -1.5 1,274,474 - Pf6N2E2_1088 0.61 +0.5 1,274,540 + Pf6N2E2_1088 0.62 +0.5 1,274,540 + Pf6N2E2_1088 0.62 -2.5 1,274,656 - Pf6N2E2_1088 0.65 +0.5 1,274,720 - Pf6N2E2_1088 0.66 -0.5 1,274,808 - Pf6N2E2_1088 0.68 +0.9 1,274,808 - Pf6N2E2_1088 0.68 -1.5 1,274,923 - Pf6N2E2_1088 0.70 -2.0 1,274,938 + Pf6N2E2_1088 0.70 -3.0 1,274,938 + Pf6N2E2_1088 0.70 +0.5 1,274,938 + Pf6N2E2_1088 0.70 +2.0 1,274,939 - Pf6N2E2_1088 0.70 -0.2 1,274,939 - Pf6N2E2_1088 0.70 -0.9 1,274,939 - Pf6N2E2_1088 0.70 -3.5 1,275,005 + Pf6N2E2_1088 0.72 -2.1 1,275,006 - Pf6N2E2_1088 0.72 -0.9 1,275,015 + Pf6N2E2_1088 0.72 -1.5 1,275,143 + Pf6N2E2_1088 0.75 +0.5 1,275,143 + Pf6N2E2_1088 0.75 +1.5 1,275,214 + Pf6N2E2_1088 0.76 -1.1 1,275,214 + -0.5 1,275,242 + Pf6N2E2_1088 0.77 +0.5 1,275,243 - Pf6N2E2_1088 0.77 +0.8 1,275,243 - Pf6N2E2_1088 0.77 +1.5 1,275,248 + Pf6N2E2_1088 0.77 +1.2 1,275,248 + Pf6N2E2_1088 0.77 -0.2 1,275,248 + Pf6N2E2_1088 0.77 +0.5 1,275,249 - Pf6N2E2_1088 0.77 +1.0 1,275,290 - Pf6N2E2_1088 0.78 +0.5 1,275,483 + Pf6N2E2_1088 0.82 -0.1 1,275,484 - Pf6N2E2_1088 0.82 -0.5 1,275,484 - Pf6N2E2_1088 0.82 +0.5 1,275,485 + Pf6N2E2_1088 0.82 -1.1 1,275,485 + Pf6N2E2_1088 0.82 -0.5 1,275,485 + Pf6N2E2_1088 0.82 -0.7 1,275,485 + Pf6N2E2_1088 0.82 +0.5 1,275,485 + Pf6N2E2_1088 0.82 -0.2 1,275,485 + Pf6N2E2_1088 0.82 -1.1 1,275,485 + Pf6N2E2_1088 0.82 -1.1 1,275,485 + Pf6N2E2_1088 0.82 -0.5 1,275,486 - Pf6N2E2_1088 0.82 -1.5 1,275,531 + Pf6N2E2_1088 0.83 +1.6 1,275,665 + Pf6N2E2_1088 0.85 -0.5 1,275,817 + Pf6N2E2_1088 0.89 +0.9 1,275,817 + Pf6N2E2_1088 0.89 -1.4 1,275,818 - Pf6N2E2_1088 0.89 -0.5 1,276,330 + -0.7 1,276,343 - +0.5 1,276,454 + -0.5 1,276,454 + -2.4 1,276,458 + -0.5 1,276,458 + -0.2 1,276,458 + -1.5 1,276,458 + -1.9 1,276,458 + +0.2 1,276,458 + -0.7 1,276,458 + +1.8 1,276,459 - +0.5 1,276,459 - +0.9 1,276,459 - +0.0 1,276,459 - -1.9 1,276,459 - -0.5 1,276,542 + -1.9 1,276,543 - -1.1 1,276,563 + -1.1 1,276,563 + -0.7 1,276,574 + +0.7 1,276,575 - -1.9 1,276,787 - Pf6N2E2_1089 0.18 +0.6 1,276,787 - Pf6N2E2_1089 0.18 +0.5 1,277,017 + Pf6N2E2_1089 0.40 +0.6
Or see this region's nucleotide sequence