Experiment: RCH2 defined with Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1030 and Pf6N2E2_1031 are separated by 320 nucleotides Pf6N2E2_1031 and Pf6N2E2_1032 are separated by 1 nucleotides Pf6N2E2_1032 and Pf6N2E2_1033 are separated by 25 nucleotides
Pf6N2E2_1030 - Alkanesulfonate monooxygenase (EC 1.14.14.5), at 1,203,869 to 1,204,957
_1030
Pf6N2E2_1031 - Alkanesulfonates transport system permease protein, at 1,205,278 to 1,206,222
_1031
Pf6N2E2_1032 - Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB, at 1,206,224 to 1,207,036
_1032
Pf6N2E2_1033 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,207,062 to 1,208,087
_1033
Position (kb)
1205
1206
1207 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1204.359 kb on + strand, within Pf6N2E2_1030 at 1204.380 kb on + strand, within Pf6N2E2_1030 at 1204.381 kb on - strand, within Pf6N2E2_1030 at 1204.438 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.448 kb on - strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.500 kb on + strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.501 kb on - strand, within Pf6N2E2_1030 at 1204.512 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.599 kb on + strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.600 kb on - strand, within Pf6N2E2_1030 at 1204.872 kb on + strand at 1204.873 kb on - strand at 1205.071 kb on + strand at 1205.077 kb on - strand at 1205.320 kb on + strand at 1205.321 kb on - strand at 1205.655 kb on - strand, within Pf6N2E2_1031 at 1205.655 kb on - strand, within Pf6N2E2_1031 at 1205.860 kb on + strand, within Pf6N2E2_1031 at 1205.860 kb on + strand, within Pf6N2E2_1031 at 1205.887 kb on - strand, within Pf6N2E2_1031 at 1206.032 kb on + strand, within Pf6N2E2_1031 at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.133 kb on + strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.134 kb on - strand at 1206.224 kb on - strand at 1206.757 kb on - strand, within Pf6N2E2_1032 at 1207.000 kb on + strand at 1207.006 kb on + strand at 1207.006 kb on + strand at 1207.007 kb on - strand at 1207.007 kb on - strand at 1207.035 kb on - strand at 1207.035 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.04 mM remove 1,204,359 + Pf6N2E2_1030 0.45 +1.3 1,204,380 + Pf6N2E2_1030 0.47 -1.1 1,204,381 - Pf6N2E2_1030 0.47 +1.0 1,204,438 - Pf6N2E2_1030 0.52 -1.5 1,204,448 - Pf6N2E2_1030 0.53 +2.4 1,204,448 - Pf6N2E2_1030 0.53 +0.5 1,204,448 - Pf6N2E2_1030 0.53 -0.3 1,204,500 + Pf6N2E2_1030 0.58 -3.3 1,204,500 + Pf6N2E2_1030 0.58 +0.5 1,204,500 + Pf6N2E2_1030 0.58 +1.6 1,204,501 - Pf6N2E2_1030 0.58 -1.5 1,204,501 - Pf6N2E2_1030 0.58 +0.4 1,204,501 - Pf6N2E2_1030 0.58 -3.2 1,204,501 - Pf6N2E2_1030 0.58 +1.3 1,204,512 + Pf6N2E2_1030 0.59 -1.3 1,204,599 + Pf6N2E2_1030 0.67 +0.0 1,204,599 + Pf6N2E2_1030 0.67 +1.5 1,204,599 + Pf6N2E2_1030 0.67 -0.5 1,204,599 + Pf6N2E2_1030 0.67 -0.2 1,204,599 + Pf6N2E2_1030 0.67 +2.1 1,204,599 + Pf6N2E2_1030 0.67 +0.2 1,204,600 - Pf6N2E2_1030 0.67 -0.0 1,204,600 - Pf6N2E2_1030 0.67 +2.1 1,204,600 - Pf6N2E2_1030 0.67 -0.5 1,204,600 - Pf6N2E2_1030 0.67 +1.5 1,204,872 + -1.5 1,204,873 - +1.1 1,205,071 + -2.8 1,205,077 - -3.8 1,205,320 + +1.5 1,205,321 - +0.5 1,205,655 - Pf6N2E2_1031 0.40 -2.5 1,205,655 - Pf6N2E2_1031 0.40 -2.6 1,205,860 + Pf6N2E2_1031 0.62 -0.0 1,205,860 + Pf6N2E2_1031 0.62 -0.3 1,205,887 - Pf6N2E2_1031 0.64 -2.1 1,206,032 + Pf6N2E2_1031 0.80 -0.5 1,206,133 + -0.0 1,206,133 + -1.5 1,206,133 + +0.8 1,206,133 + -0.5 1,206,134 - -1.8 1,206,134 - -0.5 1,206,134 - +0.5 1,206,134 - +0.9 1,206,134 - +4.0 1,206,224 - -0.5 1,206,757 - Pf6N2E2_1032 0.66 +1.3 1,207,000 + -2.7 1,207,006 + -0.6 1,207,006 + -1.5 1,207,007 - -2.4 1,207,007 - +0.1 1,207,035 - +0.3 1,207,035 - -0.2
Or see this region's nucleotide sequence