Experiment: KB with Oxymatrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3980 and Psyr_3981 are separated by 35 nucleotides Psyr_3981 and Psyr_3982 are separated by 126 nucleotides Psyr_3982 and Psyr_RNA61 are separated by 158 nucleotides Psyr_RNA61 and Psyr_3983 are separated by 361 nucleotides
Psyr_3980: Psyr_3980 - hypothetical protein, at 4,733,916 to 4,734,152
_3980
Psyr_3981: Psyr_3981 - ribosomal small subunit pseudouridine synthase A, at 4,734,188 to 4,734,880
_3981
Psyr_3982: Psyr_3982 - 3-hydroxyacyl-CoA-acyl carrier protein transferase, at 4,735,007 to 4,735,888
_3982
Psyr_RNA61: Psyr_RNA61 - tRNA-Gly, at 4,736,047 to 4,736,120
_RNA61
Psyr_3983: Psyr_3983 - Serralysin, at 4,736,482 to 4,738,839
_3983
Position (kb)
4735
4736 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.150 kb on + strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.151 kb on - strand at 4734.273 kb on + strand, within Psyr_3981 at 4734.371 kb on + strand, within Psyr_3981 at 4734.372 kb on - strand, within Psyr_3981 at 4734.581 kb on + strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.582 kb on - strand, within Psyr_3981 at 4734.584 kb on + strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.585 kb on - strand, within Psyr_3981 at 4734.645 kb on - strand, within Psyr_3981 at 4734.691 kb on - strand, within Psyr_3981 at 4734.842 kb on + strand at 4734.843 kb on - strand at 4734.980 kb on + strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4734.981 kb on - strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.006 kb on + strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.007 kb on - strand at 4735.055 kb on + strand at 4735.056 kb on - strand at 4735.181 kb on + strand, within Psyr_3982 at 4735.194 kb on - strand, within Psyr_3982 at 4735.418 kb on + strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.461 kb on - strand, within Psyr_3982 at 4735.508 kb on - strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.578 kb on - strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4736.015 kb on + strand at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.059 kb on + strand, within Psyr_RNA61 at 4736.066 kb on - strand, within Psyr_RNA61 at 4736.088 kb on + strand, within Psyr_RNA61 at 4736.089 kb on - strand, within Psyr_RNA61 at 4736.182 kb on + strand at 4736.182 kb on + strand at 4736.297 kb on - strand at 4736.297 kb on - strand at 4736.304 kb on + strand at 4736.356 kb on + strand at 4736.356 kb on + strand at 4736.357 kb on - strand at 4736.411 kb on + strand at 4736.411 kb on + strand at 4736.664 kb on - strand at 4736.685 kb on + strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.830 kb on - strand, within Psyr_3983 at 4736.830 kb on - strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Oxymatrine 10 mM remove 4,734,150 + -1.0 4,734,150 + +0.4 4,734,150 + +1.0 4,734,151 - +0.2 4,734,151 - -0.4 4,734,151 - +0.3 4,734,151 - -0.0 4,734,151 - +0.2 4,734,151 - -0.3 4,734,273 + Psyr_3981 0.12 -0.7 4,734,371 + Psyr_3981 0.26 -1.6 4,734,372 - Psyr_3981 0.27 -2.1 4,734,581 + Psyr_3981 0.57 -0.2 4,734,582 - Psyr_3981 0.57 +0.8 4,734,582 - Psyr_3981 0.57 -0.7 4,734,582 - Psyr_3981 0.57 +0.4 4,734,582 - Psyr_3981 0.57 -0.4 4,734,584 + Psyr_3981 0.57 -0.6 4,734,585 - Psyr_3981 0.57 -0.0 4,734,585 - Psyr_3981 0.57 +1.2 4,734,645 - Psyr_3981 0.66 +0.7 4,734,691 - Psyr_3981 0.73 -1.4 4,734,842 + -0.8 4,734,843 - -1.5 4,734,980 + +0.8 4,734,981 - -0.8 4,734,981 - -0.1 4,734,981 - +1.4 4,734,981 - +1.5 4,734,981 - -0.9 4,735,006 + +1.6 4,735,006 + +0.9 4,735,006 + +0.8 4,735,006 + +0.3 4,735,007 - +0.0 4,735,007 - -0.6 4,735,007 - -1.2 4,735,007 - +1.2 4,735,007 - +0.6 4,735,007 - +1.4 4,735,055 + -1.3 4,735,056 - -0.6 4,735,181 + Psyr_3982 0.20 +0.1 4,735,194 - Psyr_3982 0.21 -2.3 4,735,418 + Psyr_3982 0.47 +0.3 4,735,419 - Psyr_3982 0.47 -0.3 4,735,419 - Psyr_3982 0.47 +0.9 4,735,461 - Psyr_3982 0.51 -0.7 4,735,508 - Psyr_3982 0.57 +0.7 4,735,537 + Psyr_3982 0.60 +0.2 4,735,537 + Psyr_3982 0.60 +0.7 4,735,537 + Psyr_3982 0.60 +1.7 4,735,562 + Psyr_3982 0.63 +0.3 4,735,562 + Psyr_3982 0.63 +0.3 4,735,578 - Psyr_3982 0.65 +0.1 4,735,616 + Psyr_3982 0.69 +0.8 4,735,616 + Psyr_3982 0.69 +0.2 4,735,616 + Psyr_3982 0.69 +0.8 4,735,617 - Psyr_3982 0.69 -0.2 4,735,617 - Psyr_3982 0.69 +0.6 4,735,617 - Psyr_3982 0.69 +0.1 4,735,617 - Psyr_3982 0.69 -0.3 4,735,617 - Psyr_3982 0.69 -0.4 4,735,617 - Psyr_3982 0.69 +0.2 4,735,679 + Psyr_3982 0.76 -0.5 4,735,679 + Psyr_3982 0.76 +0.2 4,735,679 + Psyr_3982 0.76 +0.3 4,735,679 + Psyr_3982 0.76 -0.8 4,735,679 + Psyr_3982 0.76 -0.0 4,735,680 - Psyr_3982 0.76 +0.5 4,735,680 - Psyr_3982 0.76 -0.3 4,735,680 - Psyr_3982 0.76 +0.2 4,735,680 - Psyr_3982 0.76 -1.3 4,735,680 - Psyr_3982 0.76 -0.1 4,735,680 - Psyr_3982 0.76 +0.8 4,736,015 + -0.8 4,736,055 - Psyr_RNA61 0.11 -1.4 4,736,055 - Psyr_RNA61 0.11 -3.0 4,736,055 - Psyr_RNA61 0.11 +0.4 4,736,055 - Psyr_RNA61 0.11 -0.1 4,736,059 + Psyr_RNA61 0.16 -0.9 4,736,066 - Psyr_RNA61 0.26 -1.8 4,736,088 + Psyr_RNA61 0.55 -1.3 4,736,089 - Psyr_RNA61 0.57 +0.3 4,736,182 + -0.8 4,736,182 + -0.4 4,736,297 - +0.3 4,736,297 - +0.1 4,736,304 + +1.0 4,736,356 + -1.4 4,736,356 + -1.8 4,736,357 - -0.5 4,736,411 + -0.4 4,736,411 + -0.1 4,736,664 - +1.8 4,736,685 + +0.4 4,736,686 - +0.1 4,736,686 - +0.3 4,736,686 - -0.1 4,736,686 - -0.4 4,736,830 - Psyr_3983 0.15 +1.3 4,736,830 - Psyr_3983 0.15 -0.5 4,736,831 + Psyr_3983 0.15 -0.5 4,736,831 + Psyr_3983 0.15 +0.8 4,736,831 + Psyr_3983 0.15 +0.3 4,736,832 - Psyr_3983 0.15 -0.1 4,736,832 - Psyr_3983 0.15 +1.1
Or see this region's nucleotide sequence