Experiment: KB with Oxymatrine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0704 and Psyr_0705 are separated by 25 nucleotides Psyr_0705 and Psyr_0706 are separated by 468 nucleotides
Psyr_0704: Psyr_0704 - glutamate 5-kinase, at 806,935 to 808,053
_0704
Psyr_0705: Psyr_0705 - CreA, at 808,079 to 808,549
_0705
Psyr_0706: Psyr_0706 - Acyltransferase 3, at 809,018 to 810,079
_0706
Position (kb) 808
809 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 807.237 kb on + strand, within Psyr_0704 at 807.237 kb on + strand, within Psyr_0704 at 807.238 kb on - strand, within Psyr_0704 at 807.512 kb on - strand, within Psyr_0704 at 807.636 kb on + strand, within Psyr_0704 at 807.636 kb on + strand, within Psyr_0704 at 807.890 kb on + strand, within Psyr_0704 at 807.891 kb on - strand, within Psyr_0704 at 807.933 kb on + strand, within Psyr_0704 at 807.933 kb on + strand, within Psyr_0704 at 807.937 kb on + strand, within Psyr_0704 at 807.937 kb on + strand, within Psyr_0704 at 807.937 kb on + strand, within Psyr_0704 at 807.937 kb on + strand, within Psyr_0704 at 807.937 kb on + strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 807.938 kb on - strand, within Psyr_0704 at 808.003 kb on + strand at 808.003 kb on + strand at 808.003 kb on + strand at 808.003 kb on + strand at 808.003 kb on + strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.004 kb on - strand at 808.005 kb on + strand at 808.006 kb on - strand at 808.006 kb on - strand at 808.099 kb on - strand at 808.241 kb on + strand, within Psyr_0705 at 808.241 kb on + strand, within Psyr_0705 at 808.241 kb on + strand, within Psyr_0705 at 808.241 kb on + strand, within Psyr_0705 at 808.242 kb on - strand, within Psyr_0705 at 808.242 kb on - strand, within Psyr_0705 at 808.242 kb on - strand, within Psyr_0705 at 808.387 kb on + strand, within Psyr_0705 at 808.388 kb on - strand, within Psyr_0705 at 808.454 kb on + strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.455 kb on - strand, within Psyr_0705 at 808.463 kb on + strand, within Psyr_0705 at 808.464 kb on - strand, within Psyr_0705 at 808.486 kb on + strand, within Psyr_0705 at 808.637 kb on + strand at 808.637 kb on + strand at 808.637 kb on + strand at 808.637 kb on + strand at 808.637 kb on + strand at 808.638 kb on - strand at 808.638 kb on - strand at 808.638 kb on - strand at 808.638 kb on - strand at 808.638 kb on - strand at 808.655 kb on + strand at 808.675 kb on + strand at 808.753 kb on + strand at 808.753 kb on + strand at 808.819 kb on + strand at 808.820 kb on - strand at 808.864 kb on + strand at 808.864 kb on + strand at 808.877 kb on - strand at 808.918 kb on + strand at 808.919 kb on - strand at 808.919 kb on - strand at 808.922 kb on - strand at 808.948 kb on - strand at 808.948 kb on - strand at 808.950 kb on + strand at 808.951 kb on - strand at 808.951 kb on - strand at 808.951 kb on - strand at 808.951 kb on - strand at 808.954 kb on + strand at 809.039 kb on + strand at 809.039 kb on + strand at 809.040 kb on - strand at 809.061 kb on + strand at 809.061 kb on + strand at 809.062 kb on - strand at 809.062 kb on - strand at 809.080 kb on + strand at 809.116 kb on + strand at 809.116 kb on + strand at 809.116 kb on + strand at 809.116 kb on + strand at 809.116 kb on + strand at 809.117 kb on - strand at 809.117 kb on - strand at 809.117 kb on - strand at 809.117 kb on - strand at 809.117 kb on - strand at 809.117 kb on - strand at 809.118 kb on + strand at 809.118 kb on + strand at 809.118 kb on + strand at 809.119 kb on - strand at 809.119 kb on - strand at 809.155 kb on - strand, within Psyr_0706 at 809.155 kb on - strand, within Psyr_0706 at 809.184 kb on - strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.233 kb on + strand, within Psyr_0706 at 809.234 kb on - strand, within Psyr_0706 at 809.234 kb on - strand, within Psyr_0706 at 809.236 kb on - strand, within Psyr_0706 at 809.310 kb on + strand, within Psyr_0706 at 809.311 kb on - strand, within Psyr_0706 at 809.311 kb on - strand, within Psyr_0706 at 809.311 kb on - strand, within Psyr_0706 at 809.311 kb on - strand, within Psyr_0706 at 809.321 kb on + strand, within Psyr_0706 at 809.339 kb on + strand, within Psyr_0706 at 809.339 kb on + strand, within Psyr_0706 at 809.340 kb on - strand, within Psyr_0706 at 809.341 kb on + strand, within Psyr_0706 at 809.341 kb on + strand, within Psyr_0706 at 809.342 kb on - strand, within Psyr_0706 at 809.399 kb on + strand, within Psyr_0706 at 809.399 kb on + strand, within Psyr_0706 at 809.399 kb on + strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.400 kb on - strand, within Psyr_0706 at 809.414 kb on + strand, within Psyr_0706 at 809.415 kb on - strand, within Psyr_0706 at 809.415 kb on - strand, within Psyr_0706 at 809.421 kb on + strand, within Psyr_0706 at 809.421 kb on + strand, within Psyr_0706 at 809.421 kb on + strand, within Psyr_0706 at 809.421 kb on + strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.422 kb on - strand, within Psyr_0706 at 809.428 kb on + strand, within Psyr_0706 at 809.428 kb on + strand, within Psyr_0706 at 809.428 kb on + strand, within Psyr_0706 at 809.429 kb on - strand, within Psyr_0706 at 809.462 kb on + strand, within Psyr_0706 at 809.462 kb on + strand, within Psyr_0706 at 809.462 kb on + strand, within Psyr_0706 at 809.463 kb on - strand, within Psyr_0706 at 809.463 kb on - strand, within Psyr_0706 at 809.511 kb on - strand, within Psyr_0706 at 809.511 kb on - strand, within Psyr_0706 at 809.513 kb on + strand, within Psyr_0706 at 809.513 kb on + strand, within Psyr_0706 at 809.513 kb on + strand, within Psyr_0706 at 809.514 kb on - strand, within Psyr_0706 at 809.514 kb on - strand, within Psyr_0706 at 809.514 kb on - strand, within Psyr_0706
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Oxymatrine 10 mM remove 807,237 + Psyr_0704 0.27 -2.2 807,237 + Psyr_0704 0.27 -0.1 807,238 - Psyr_0704 0.27 -0.2 807,512 - Psyr_0704 0.52 +0.5 807,636 + Psyr_0704 0.63 -0.6 807,636 + Psyr_0704 0.63 -0.3 807,890 + Psyr_0704 0.85 -0.4 807,891 - Psyr_0704 0.85 +0.0 807,933 + Psyr_0704 0.89 -1.0 807,933 + Psyr_0704 0.89 +1.0 807,937 + Psyr_0704 0.90 -0.4 807,937 + Psyr_0704 0.90 +1.6 807,937 + Psyr_0704 0.90 -0.1 807,937 + Psyr_0704 0.90 +1.1 807,937 + Psyr_0704 0.90 -0.1 807,938 - Psyr_0704 0.90 -1.1 807,938 - Psyr_0704 0.90 +0.0 807,938 - Psyr_0704 0.90 +0.6 807,938 - Psyr_0704 0.90 -0.5 807,938 - Psyr_0704 0.90 +1.2 807,938 - Psyr_0704 0.90 -1.4 807,938 - Psyr_0704 0.90 +0.1 807,938 - Psyr_0704 0.90 -0.8 807,938 - Psyr_0704 0.90 -0.4 807,938 - Psyr_0704 0.90 +1.5 807,938 - Psyr_0704 0.90 -0.1 807,938 - Psyr_0704 0.90 -0.3 807,938 - Psyr_0704 0.90 +0.0 807,938 - Psyr_0704 0.90 -0.2 807,938 - Psyr_0704 0.90 -1.1 807,938 - Psyr_0704 0.90 -1.0 807,938 - Psyr_0704 0.90 -0.8 808,003 + +0.2 808,003 + +0.3 808,003 + -0.0 808,003 + +0.1 808,003 + +0.1 808,004 - +0.5 808,004 - -0.5 808,004 - +0.4 808,004 - -0.4 808,004 - -0.4 808,004 - +0.0 808,004 - +0.1 808,004 - -0.3 808,004 - +0.6 808,004 - +0.2 808,005 + -0.4 808,006 - -0.7 808,006 - -0.0 808,099 - -0.5 808,241 + Psyr_0705 0.34 +1.1 808,241 + Psyr_0705 0.34 -0.4 808,241 + Psyr_0705 0.34 +0.9 808,241 + Psyr_0705 0.34 -0.4 808,242 - Psyr_0705 0.35 -2.0 808,242 - Psyr_0705 0.35 +0.1 808,242 - Psyr_0705 0.35 -0.6 808,387 + Psyr_0705 0.65 +1.6 808,388 - Psyr_0705 0.66 -0.9 808,454 + Psyr_0705 0.80 -0.8 808,455 - Psyr_0705 0.80 -2.4 808,455 - Psyr_0705 0.80 -0.4 808,455 - Psyr_0705 0.80 -0.5 808,455 - Psyr_0705 0.80 -0.6 808,455 - Psyr_0705 0.80 -0.4 808,455 - Psyr_0705 0.80 -0.8 808,455 - Psyr_0705 0.80 +0.5 808,463 + Psyr_0705 0.82 -0.2 808,464 - Psyr_0705 0.82 -0.3 808,486 + Psyr_0705 0.86 +0.2 808,637 + +0.9 808,637 + +0.1 808,637 + -0.5 808,637 + -0.8 808,637 + +1.1 808,638 - +0.7 808,638 - +0.5 808,638 - -0.2 808,638 - +0.7 808,638 - +0.7 808,655 + +0.5 808,675 + -1.1 808,753 + -0.4 808,753 + -1.2 808,819 + +0.2 808,820 - +0.4 808,864 + -0.8 808,864 + -0.3 808,877 - +2.5 808,918 + -0.5 808,919 - -0.0 808,919 - +0.2 808,922 - -0.5 808,948 - -0.3 808,948 - +0.6 808,950 + -0.6 808,951 - -1.0 808,951 - -0.2 808,951 - -0.4 808,951 - -0.5 808,954 + -0.7 809,039 + -0.7 809,039 + +0.3 809,040 - -0.5 809,061 + -0.8 809,061 + +1.0 809,062 - +1.2 809,062 - -0.1 809,080 + +0.6 809,116 + -0.9 809,116 + -0.4 809,116 + -0.0 809,116 + +0.6 809,116 + +0.2 809,117 - +0.2 809,117 - -0.4 809,117 - -0.1 809,117 - -0.6 809,117 - +0.4 809,117 - -0.9 809,118 + +0.9 809,118 + +0.2 809,118 + +0.6 809,119 - -1.2 809,119 - -0.1 809,155 - Psyr_0706 0.13 -1.6 809,155 - Psyr_0706 0.13 -1.7 809,184 - Psyr_0706 0.16 -0.4 809,233 + Psyr_0706 0.20 +0.1 809,233 + Psyr_0706 0.20 -0.9 809,233 + Psyr_0706 0.20 -0.8 809,233 + Psyr_0706 0.20 -0.1 809,233 + Psyr_0706 0.20 +0.4 809,233 + Psyr_0706 0.20 +2.0 809,233 + Psyr_0706 0.20 +0.2 809,234 - Psyr_0706 0.20 -0.4 809,234 - Psyr_0706 0.20 -0.3 809,236 - Psyr_0706 0.21 -1.8 809,310 + Psyr_0706 0.27 -0.8 809,311 - Psyr_0706 0.28 +0.9 809,311 - Psyr_0706 0.28 -1.6 809,311 - Psyr_0706 0.28 -1.7 809,311 - Psyr_0706 0.28 +0.3 809,321 + Psyr_0706 0.29 -0.1 809,339 + Psyr_0706 0.30 -1.1 809,339 + Psyr_0706 0.30 -0.1 809,340 - Psyr_0706 0.30 -1.5 809,341 + Psyr_0706 0.30 -0.9 809,341 + Psyr_0706 0.30 -0.3 809,342 - Psyr_0706 0.31 +0.0 809,399 + Psyr_0706 0.36 -0.6 809,399 + Psyr_0706 0.36 -0.1 809,399 + Psyr_0706 0.36 -1.9 809,400 - Psyr_0706 0.36 +0.2 809,400 - Psyr_0706 0.36 +0.1 809,400 - Psyr_0706 0.36 +0.6 809,400 - Psyr_0706 0.36 -0.0 809,400 - Psyr_0706 0.36 -0.7 809,400 - Psyr_0706 0.36 -0.6 809,400 - Psyr_0706 0.36 -0.3 809,400 - Psyr_0706 0.36 -0.5 809,414 + Psyr_0706 0.37 -1.9 809,415 - Psyr_0706 0.37 -0.6 809,415 - Psyr_0706 0.37 -0.9 809,421 + Psyr_0706 0.38 -1.9 809,421 + Psyr_0706 0.38 -0.5 809,421 + Psyr_0706 0.38 +0.9 809,421 + Psyr_0706 0.38 +0.4 809,422 - Psyr_0706 0.38 -0.6 809,422 - Psyr_0706 0.38 +0.4 809,422 - Psyr_0706 0.38 +0.2 809,422 - Psyr_0706 0.38 -0.8 809,422 - Psyr_0706 0.38 -0.3 809,422 - Psyr_0706 0.38 -0.4 809,428 + Psyr_0706 0.39 -1.4 809,428 + Psyr_0706 0.39 -0.8 809,428 + Psyr_0706 0.39 -0.7 809,429 - Psyr_0706 0.39 -1.8 809,462 + Psyr_0706 0.42 +0.6 809,462 + Psyr_0706 0.42 +0.4 809,462 + Psyr_0706 0.42 -0.1 809,463 - Psyr_0706 0.42 -0.0 809,463 - Psyr_0706 0.42 -0.5 809,511 - Psyr_0706 0.46 -1.0 809,511 - Psyr_0706 0.46 +0.6 809,513 + Psyr_0706 0.47 -0.0 809,513 + Psyr_0706 0.47 +0.6 809,513 + Psyr_0706 0.47 +0.1 809,514 - Psyr_0706 0.47 -0.3 809,514 - Psyr_0706 0.47 +3.2 809,514 - Psyr_0706 0.47 -0.6
Or see this region's nucleotide sequence