Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_649 and Pf6N2E2_650 are separated by 16 nucleotides Pf6N2E2_650 and Pf6N2E2_651 overlap by 4 nucleotides Pf6N2E2_651 and Pf6N2E2_652 are separated by 172 nucleotides
Pf6N2E2_649 - General secretion pathway protein L, at 737,663 to 738,835
_649
Pf6N2E2_650 - General secretion pathway protein M, at 738,852 to 739,400
_650
Pf6N2E2_651 - General secretion pathway protein D, at 739,397 to 741,760
_651
Pf6N2E2_652 - General secretion pathway protein E, at 741,933 to 743,351
_652
Position (kb) 739
740
741
742 Strain fitness (log2 ratio) -2
-1
0
1
2 at 738.413 kb on + strand, within Pf6N2E2_649 at 738.834 kb on - strand at 738.834 kb on - strand at 739.008 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.152 kb on + strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.153 kb on - strand, within Pf6N2E2_650 at 739.156 kb on + strand, within Pf6N2E2_650 at 739.157 kb on - strand, within Pf6N2E2_650 at 739.597 kb on + strand at 739.946 kb on + strand, within Pf6N2E2_651 at 739.947 kb on - strand, within Pf6N2E2_651 at 740.004 kb on - strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.354 kb on + strand, within Pf6N2E2_651 at 740.402 kb on + strand, within Pf6N2E2_651 at 740.522 kb on - strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.155 kb on + strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.156 kb on - strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.278 kb on + strand, within Pf6N2E2_651 at 741.279 kb on - strand, within Pf6N2E2_651 at 741.530 kb on + strand at 741.530 kb on + strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.531 kb on - strand at 741.535 kb on + strand at 741.565 kb on + strand at 741.622 kb on + strand at 741.823 kb on + strand at 741.824 kb on - strand at 741.917 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.948 kb on + strand at 741.976 kb on + strand at 742.122 kb on + strand, within Pf6N2E2_652 at 742.123 kb on - strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.701 kb on + strand, within Pf6N2E2_652 at 742.702 kb on - strand, within Pf6N2E2_652
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 738,413 + Pf6N2E2_649 0.64 -0.4 738,834 - +0.3 738,834 - +0.9 739,008 + Pf6N2E2_650 0.28 +0.2 739,152 + Pf6N2E2_650 0.55 +0.9 739,152 + Pf6N2E2_650 0.55 -0.1 739,153 - Pf6N2E2_650 0.55 +0.7 739,153 - Pf6N2E2_650 0.55 +0.6 739,153 - Pf6N2E2_650 0.55 +1.0 739,156 + Pf6N2E2_650 0.55 -0.7 739,157 - Pf6N2E2_650 0.56 +0.2 739,597 + -1.2 739,946 + Pf6N2E2_651 0.23 -0.2 739,947 - Pf6N2E2_651 0.23 +0.6 740,004 - Pf6N2E2_651 0.26 +1.1 740,354 + Pf6N2E2_651 0.40 -0.7 740,354 + Pf6N2E2_651 0.40 +1.4 740,354 + Pf6N2E2_651 0.40 +0.3 740,402 + Pf6N2E2_651 0.43 -1.3 740,522 - Pf6N2E2_651 0.48 +0.2 741,155 + Pf6N2E2_651 0.74 -1.3 741,155 + Pf6N2E2_651 0.74 +0.3 741,155 + Pf6N2E2_651 0.74 +0.1 741,156 - Pf6N2E2_651 0.74 +1.0 741,156 - Pf6N2E2_651 0.74 +0.2 741,156 - Pf6N2E2_651 0.74 +1.1 741,156 - Pf6N2E2_651 0.74 +0.7 741,278 + Pf6N2E2_651 0.80 +0.7 741,278 + Pf6N2E2_651 0.80 -0.7 741,278 + Pf6N2E2_651 0.80 +0.6 741,278 + Pf6N2E2_651 0.80 +0.9 741,279 - Pf6N2E2_651 0.80 +0.2 741,530 + +0.6 741,530 + -0.3 741,531 - -1.2 741,531 - +0.0 741,531 - +1.3 741,535 + +1.5 741,565 + +1.3 741,622 + +0.9 741,823 + -1.7 741,824 - +2.6 741,917 + +0.9 741,948 + +1.9 741,948 + +0.9 741,948 + -0.3 741,976 + -1.2 742,122 + Pf6N2E2_652 0.13 -0.4 742,123 - Pf6N2E2_652 0.13 -0.1 742,701 + Pf6N2E2_652 0.54 -0.4 742,701 + Pf6N2E2_652 0.54 -1.5 742,701 + Pf6N2E2_652 0.54 -1.9 742,702 - Pf6N2E2_652 0.54 +0.2
Or see this region's nucleotide sequence