Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5739

Experiment: RCH2 defined with Cobalt chloride 0.02 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5738 and Pf6N2E2_5739 overlap by 4 nucleotidesPf6N2E2_5739 and Pf6N2E2_5740 overlap by 19 nucleotidesPf6N2E2_5740 and Pf6N2E2_5741 overlap by 8 nucleotides Pf6N2E2_5738 - Cobyrinic acid A,C-diamide synthase, at 6,554,871 to 6,556,187 _5738 Pf6N2E2_5739 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 6,556,184 to 6,556,834 _5739 Pf6N2E2_5740 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 6,556,816 to 6,557,739 _5740 Pf6N2E2_5741 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 6,557,732 to 6,558,724 _5741 Position (kb) 6556 6557Strain fitness (log2 ratio) -3 -2 -1 0 1at 6555.624 kb on + strand, within Pf6N2E2_5738at 6555.624 kb on + strand, within Pf6N2E2_5738at 6555.625 kb on - strand, within Pf6N2E2_5738at 6555.625 kb on - strand, within Pf6N2E2_5738at 6555.625 kb on - strand, within Pf6N2E2_5738at 6555.721 kb on - strand, within Pf6N2E2_5738at 6555.839 kb on + strand, within Pf6N2E2_5738at 6555.846 kb on + strand, within Pf6N2E2_5738at 6555.846 kb on + strand, within Pf6N2E2_5738at 6555.846 kb on + strand, within Pf6N2E2_5738at 6555.847 kb on - strand, within Pf6N2E2_5738at 6555.847 kb on - strand, within Pf6N2E2_5738at 6555.847 kb on - strand, within Pf6N2E2_5738at 6555.989 kb on + strand, within Pf6N2E2_5738at 6555.989 kb on + strand, within Pf6N2E2_5738at 6555.990 kb on - strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.993 kb on + strand, within Pf6N2E2_5738at 6555.994 kb on - strand, within Pf6N2E2_5738at 6555.994 kb on - strand, within Pf6N2E2_5738at 6555.994 kb on - strand, within Pf6N2E2_5738at 6555.994 kb on - strand, within Pf6N2E2_5738at 6555.994 kb on - strand, within Pf6N2E2_5738at 6556.044 kb on - strand, within Pf6N2E2_5738at 6556.104 kb on + strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.105 kb on - strandat 6556.226 kb on + strandat 6556.713 kb on + strand, within Pf6N2E2_5739at 6556.743 kb on - strand, within Pf6N2E2_5739at 6556.805 kb on + strandat 6556.805 kb on + strandat 6556.805 kb on + strandat 6556.805 kb on + strandat 6557.084 kb on - strand, within Pf6N2E2_5740at 6557.326 kb on + strand, within Pf6N2E2_5740at 6557.327 kb on - strand, within Pf6N2E2_5740at 6557.341 kb on - strand, within Pf6N2E2_5740at 6557.341 kb on - strand, within Pf6N2E2_5740at 6557.425 kb on + strand, within Pf6N2E2_5740at 6557.425 kb on + strand, within Pf6N2E2_5740at 6557.426 kb on - strand, within Pf6N2E2_5740at 6557.426 kb on - strand, within Pf6N2E2_5740at 6557.426 kb on - strand, within Pf6N2E2_5740at 6557.455 kb on + strand, within Pf6N2E2_5740at 6557.690 kb on + strandat 6557.691 kb on - strandat 6557.691 kb on - strandat 6557.691 kb on - strandat 6557.731 kb on + strandat 6557.731 kb on + strandat 6557.731 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM
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6,555,624 + Pf6N2E2_5738 0.57 -0.2
6,555,624 + Pf6N2E2_5738 0.57 +0.8
6,555,625 - Pf6N2E2_5738 0.57 -2.8
6,555,625 - Pf6N2E2_5738 0.57 -0.7
6,555,625 - Pf6N2E2_5738 0.57 +0.8
6,555,721 - Pf6N2E2_5738 0.65 -1.7
6,555,839 + Pf6N2E2_5738 0.74 -0.2
6,555,846 + Pf6N2E2_5738 0.74 -1.5
6,555,846 + Pf6N2E2_5738 0.74 -2.4
6,555,846 + Pf6N2E2_5738 0.74 +0.8
6,555,847 - Pf6N2E2_5738 0.74 -0.1
6,555,847 - Pf6N2E2_5738 0.74 +0.8
6,555,847 - Pf6N2E2_5738 0.74 -1.1
6,555,989 + Pf6N2E2_5738 0.85 -1.2
6,555,989 + Pf6N2E2_5738 0.85 -1.2
6,555,990 - Pf6N2E2_5738 0.85 -0.2
6,555,993 + Pf6N2E2_5738 0.85 +0.5
6,555,993 + Pf6N2E2_5738 0.85 +0.8
6,555,993 + Pf6N2E2_5738 0.85 -0.2
6,555,993 + Pf6N2E2_5738 0.85 -1.8
6,555,993 + Pf6N2E2_5738 0.85 +1.0
6,555,993 + Pf6N2E2_5738 0.85 +0.8
6,555,994 - Pf6N2E2_5738 0.85 +0.8
6,555,994 - Pf6N2E2_5738 0.85 -2.0
6,555,994 - Pf6N2E2_5738 0.85 +1.0
6,555,994 - Pf6N2E2_5738 0.85 -1.0
6,555,994 - Pf6N2E2_5738 0.85 -0.2
6,556,044 - Pf6N2E2_5738 0.89 -2.2
6,556,104 + +0.0
6,556,105 - +0.6
6,556,105 - -0.4
6,556,105 - +1.6
6,556,105 - -1.6
6,556,105 - -0.8
6,556,105 - +0.3
6,556,105 - +0.8
6,556,226 + +0.1
6,556,713 + Pf6N2E2_5739 0.81 -0.8
6,556,743 - Pf6N2E2_5739 0.86 -1.3
6,556,805 + +0.5
6,556,805 + -0.1
6,556,805 + +0.8
6,556,805 + +0.8
6,557,084 - Pf6N2E2_5740 0.29 -1.4
6,557,326 + Pf6N2E2_5740 0.55 +1.1
6,557,327 - Pf6N2E2_5740 0.55 -0.7
6,557,341 - Pf6N2E2_5740 0.57 -1.2
6,557,341 - Pf6N2E2_5740 0.57 +0.8
6,557,425 + Pf6N2E2_5740 0.66 -0.2
6,557,425 + Pf6N2E2_5740 0.66 -0.6
6,557,426 - Pf6N2E2_5740 0.66 +1.2
6,557,426 - Pf6N2E2_5740 0.66 +0.4
6,557,426 - Pf6N2E2_5740 0.66 +1.3
6,557,455 + Pf6N2E2_5740 0.69 -2.7
6,557,690 + -0.2
6,557,691 - -0.2
6,557,691 - -0.8
6,557,691 - -0.2
6,557,731 + -1.9
6,557,731 + -0.4
6,557,731 + -0.5

Or see this region's nucleotide sequence