Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5738 and Pf6N2E2_5739 overlap by 4 nucleotides Pf6N2E2_5739 and Pf6N2E2_5740 overlap by 19 nucleotides Pf6N2E2_5740 and Pf6N2E2_5741 overlap by 8 nucleotides
Pf6N2E2_5738 - Cobyrinic acid A,C-diamide synthase, at 6,554,871 to 6,556,187
_5738
Pf6N2E2_5739 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 6,556,184 to 6,556,834
_5739
Pf6N2E2_5740 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 6,556,816 to 6,557,739
_5740
Pf6N2E2_5741 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 6,557,732 to 6,558,724
_5741
Position (kb)
6556
6557 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6555.624 kb on + strand, within Pf6N2E2_5738 at 6555.624 kb on + strand, within Pf6N2E2_5738 at 6555.625 kb on - strand, within Pf6N2E2_5738 at 6555.625 kb on - strand, within Pf6N2E2_5738 at 6555.625 kb on - strand, within Pf6N2E2_5738 at 6555.721 kb on - strand, within Pf6N2E2_5738 at 6555.839 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.846 kb on + strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.847 kb on - strand, within Pf6N2E2_5738 at 6555.989 kb on + strand, within Pf6N2E2_5738 at 6555.989 kb on + strand, within Pf6N2E2_5738 at 6555.990 kb on - strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.993 kb on + strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6555.994 kb on - strand, within Pf6N2E2_5738 at 6556.044 kb on - strand, within Pf6N2E2_5738 at 6556.104 kb on + strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.105 kb on - strand at 6556.226 kb on + strand at 6556.713 kb on + strand, within Pf6N2E2_5739 at 6556.743 kb on - strand, within Pf6N2E2_5739 at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6556.805 kb on + strand at 6557.084 kb on - strand, within Pf6N2E2_5740 at 6557.326 kb on + strand, within Pf6N2E2_5740 at 6557.327 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.341 kb on - strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.425 kb on + strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.426 kb on - strand, within Pf6N2E2_5740 at 6557.455 kb on + strand, within Pf6N2E2_5740 at 6557.690 kb on + strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.691 kb on - strand at 6557.731 kb on + strand at 6557.731 kb on + strand at 6557.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 6,555,624 + Pf6N2E2_5738 0.57 -0.2 6,555,624 + Pf6N2E2_5738 0.57 +0.8 6,555,625 - Pf6N2E2_5738 0.57 -2.8 6,555,625 - Pf6N2E2_5738 0.57 -0.7 6,555,625 - Pf6N2E2_5738 0.57 +0.8 6,555,721 - Pf6N2E2_5738 0.65 -1.7 6,555,839 + Pf6N2E2_5738 0.74 -0.2 6,555,846 + Pf6N2E2_5738 0.74 -1.5 6,555,846 + Pf6N2E2_5738 0.74 -2.4 6,555,846 + Pf6N2E2_5738 0.74 +0.8 6,555,847 - Pf6N2E2_5738 0.74 -0.1 6,555,847 - Pf6N2E2_5738 0.74 +0.8 6,555,847 - Pf6N2E2_5738 0.74 -1.1 6,555,989 + Pf6N2E2_5738 0.85 -1.2 6,555,989 + Pf6N2E2_5738 0.85 -1.2 6,555,990 - Pf6N2E2_5738 0.85 -0.2 6,555,993 + Pf6N2E2_5738 0.85 +0.5 6,555,993 + Pf6N2E2_5738 0.85 +0.8 6,555,993 + Pf6N2E2_5738 0.85 -0.2 6,555,993 + Pf6N2E2_5738 0.85 -1.8 6,555,993 + Pf6N2E2_5738 0.85 +1.0 6,555,993 + Pf6N2E2_5738 0.85 +0.8 6,555,994 - Pf6N2E2_5738 0.85 +0.8 6,555,994 - Pf6N2E2_5738 0.85 -2.0 6,555,994 - Pf6N2E2_5738 0.85 +1.0 6,555,994 - Pf6N2E2_5738 0.85 -1.0 6,555,994 - Pf6N2E2_5738 0.85 -0.2 6,556,044 - Pf6N2E2_5738 0.89 -2.2 6,556,104 + +0.0 6,556,105 - +0.6 6,556,105 - -0.4 6,556,105 - +1.6 6,556,105 - -1.6 6,556,105 - -0.8 6,556,105 - +0.3 6,556,105 - +0.8 6,556,226 + +0.1 6,556,713 + Pf6N2E2_5739 0.81 -0.8 6,556,743 - Pf6N2E2_5739 0.86 -1.3 6,556,805 + +0.5 6,556,805 + -0.1 6,556,805 + +0.8 6,556,805 + +0.8 6,557,084 - Pf6N2E2_5740 0.29 -1.4 6,557,326 + Pf6N2E2_5740 0.55 +1.1 6,557,327 - Pf6N2E2_5740 0.55 -0.7 6,557,341 - Pf6N2E2_5740 0.57 -1.2 6,557,341 - Pf6N2E2_5740 0.57 +0.8 6,557,425 + Pf6N2E2_5740 0.66 -0.2 6,557,425 + Pf6N2E2_5740 0.66 -0.6 6,557,426 - Pf6N2E2_5740 0.66 +1.2 6,557,426 - Pf6N2E2_5740 0.66 +0.4 6,557,426 - Pf6N2E2_5740 0.66 +1.3 6,557,455 + Pf6N2E2_5740 0.69 -2.7 6,557,690 + -0.2 6,557,691 - -0.2 6,557,691 - -0.8 6,557,691 - -0.2 6,557,731 + -1.9 6,557,731 + -0.4 6,557,731 + -0.5
Or see this region's nucleotide sequence