Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5226 and Pf6N2E2_5227 are separated by 27 nucleotides Pf6N2E2_5227 and Pf6N2E2_5228 are separated by 123 nucleotides Pf6N2E2_5228 and Pf6N2E2_5229 are separated by 12 nucleotides Pf6N2E2_5229 and Pf6N2E2_5230 overlap by 1 nucleotides
Pf6N2E2_5226 - FIG00955589: hypothetical protein, at 6,024,072 to 6,024,377
_5226
Pf6N2E2_5227 - Oxidoreductase, short-chain dehydrogenase/reductase family (EC 1.1.1.-), at 6,024,405 to 6,025,178
_5227
Pf6N2E2_5228 - Deoxyribodipyrimidine photolyase (EC 4.1.99.3), at 6,025,302 to 6,026,753
_5228
Pf6N2E2_5229 - Transcriptional regulator, MerR family, associated with photolyase, at 6,026,766 to 6,027,713
_5229
Pf6N2E2_5230 - COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA, at 6,027,713 to 6,028,675
_5230
Position (kb)
6025
6026
6027 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6024.389 kb on + strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.390 kb on - strand at 6024.638 kb on - strand, within Pf6N2E2_5227 at 6024.648 kb on + strand, within Pf6N2E2_5227 at 6024.751 kb on + strand, within Pf6N2E2_5227 at 6024.752 kb on - strand, within Pf6N2E2_5227 at 6024.771 kb on - strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.860 kb on + strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.861 kb on - strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.901 kb on + strand, within Pf6N2E2_5227 at 6024.902 kb on - strand, within Pf6N2E2_5227 at 6025.035 kb on + strand, within Pf6N2E2_5227 at 6025.036 kb on - strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.084 kb on + strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.085 kb on - strand, within Pf6N2E2_5227 at 6025.205 kb on + strand at 6025.205 kb on + strand at 6025.205 kb on + strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.206 kb on - strand at 6025.227 kb on - strand at 6025.227 kb on - strand at 6025.244 kb on + strand at 6025.245 kb on - strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.250 kb on + strand at 6025.251 kb on - strand at 6025.251 kb on - strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.252 kb on + strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.253 kb on - strand at 6025.264 kb on + strand at 6025.281 kb on - strand at 6025.439 kb on + strand at 6025.701 kb on + strand, within Pf6N2E2_5228 at 6025.887 kb on + strand, within Pf6N2E2_5228 at 6026.029 kb on + strand, within Pf6N2E2_5228 at 6026.084 kb on - strand, within Pf6N2E2_5228 at 6026.285 kb on - strand, within Pf6N2E2_5228 at 6026.285 kb on - strand, within Pf6N2E2_5228 at 6026.285 kb on - strand, within Pf6N2E2_5228 at 6026.320 kb on + strand, within Pf6N2E2_5228 at 6026.320 kb on + strand, within Pf6N2E2_5228 at 6026.321 kb on - strand, within Pf6N2E2_5228 at 6026.321 kb on - strand, within Pf6N2E2_5228 at 6026.505 kb on + strand, within Pf6N2E2_5228 at 6026.662 kb on + strand at 6026.662 kb on + strand at 6026.663 kb on - strand at 6026.718 kb on + strand at 6026.718 kb on + strand at 6026.718 kb on + strand at 6026.719 kb on - strand at 6026.767 kb on + strand at 6026.767 kb on + strand at 6026.767 kb on + strand at 6026.767 kb on + strand at 6026.767 kb on + strand at 6026.767 kb on + strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.768 kb on - strand at 6026.959 kb on + strand, within Pf6N2E2_5229 at 6027.081 kb on + strand, within Pf6N2E2_5229 at 6027.081 kb on + strand, within Pf6N2E2_5229 at 6027.081 kb on + strand, within Pf6N2E2_5229 at 6027.081 kb on + strand, within Pf6N2E2_5229 at 6027.082 kb on - strand, within Pf6N2E2_5229 at 6027.082 kb on - strand, within Pf6N2E2_5229 at 6027.082 kb on - strand, within Pf6N2E2_5229 at 6027.082 kb on - strand, within Pf6N2E2_5229 at 6027.082 kb on - strand, within Pf6N2E2_5229 at 6027.157 kb on + strand, within Pf6N2E2_5229 at 6027.157 kb on + strand, within Pf6N2E2_5229 at 6027.157 kb on + strand, within Pf6N2E2_5229 at 6027.158 kb on - strand at 6027.158 kb on - strand, within Pf6N2E2_5229 at 6027.158 kb on - strand, within Pf6N2E2_5229 at 6027.193 kb on + strand, within Pf6N2E2_5229 at 6027.194 kb on - strand, within Pf6N2E2_5229 at 6027.299 kb on - strand, within Pf6N2E2_5229 at 6027.630 kb on + strand at 6027.630 kb on + strand at 6027.630 kb on + strand at 6027.630 kb on + strand at 6027.631 kb on - strand at 6027.632 kb on + strand at 6027.633 kb on - strand at 6027.719 kb on - strand at 6027.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 6,024,389 + -1.0 6,024,390 - -0.1 6,024,390 - +1.4 6,024,390 - +0.8 6,024,390 - +0.3 6,024,390 - +0.4 6,024,638 - Pf6N2E2_5227 0.30 +1.7 6,024,648 + Pf6N2E2_5227 0.31 -0.2 6,024,751 + Pf6N2E2_5227 0.45 -1.1 6,024,752 - Pf6N2E2_5227 0.45 +0.3 6,024,771 - Pf6N2E2_5227 0.47 +1.0 6,024,860 + Pf6N2E2_5227 0.59 +0.6 6,024,860 + Pf6N2E2_5227 0.59 +1.7 6,024,860 + Pf6N2E2_5227 0.59 -0.5 6,024,860 + Pf6N2E2_5227 0.59 +0.8 6,024,860 + Pf6N2E2_5227 0.59 +0.4 6,024,860 + Pf6N2E2_5227 0.59 -0.6 6,024,861 - Pf6N2E2_5227 0.59 -1.4 6,024,861 - Pf6N2E2_5227 0.59 +0.9 6,024,861 - Pf6N2E2_5227 0.59 -2.8 6,024,861 - Pf6N2E2_5227 0.59 +0.0 6,024,901 + Pf6N2E2_5227 0.64 +0.5 6,024,901 + Pf6N2E2_5227 0.64 -0.5 6,024,901 + Pf6N2E2_5227 0.64 -0.0 6,024,902 - Pf6N2E2_5227 0.64 +0.6 6,025,035 + Pf6N2E2_5227 0.81 -0.6 6,025,036 - Pf6N2E2_5227 0.82 +0.1 6,025,084 + Pf6N2E2_5227 0.88 +0.2 6,025,084 + Pf6N2E2_5227 0.88 -0.0 6,025,084 + Pf6N2E2_5227 0.88 +0.3 6,025,085 - Pf6N2E2_5227 0.88 -1.3 6,025,085 - Pf6N2E2_5227 0.88 -1.0 6,025,085 - Pf6N2E2_5227 0.88 +0.3 6,025,205 + +0.7 6,025,205 + +1.4 6,025,205 + +0.3 6,025,206 - -0.4 6,025,206 - +1.0 6,025,206 - -0.5 6,025,206 - +1.0 6,025,206 - +0.1 6,025,227 - +0.1 6,025,227 - +1.7 6,025,244 + +1.0 6,025,245 - +0.4 6,025,250 + -0.2 6,025,250 + +1.0 6,025,250 + -0.3 6,025,250 + +2.0 6,025,250 + -1.0 6,025,250 + -0.2 6,025,250 + +0.4 6,025,251 - +2.9 6,025,251 - +0.7 6,025,252 + -0.5 6,025,252 + +1.4 6,025,252 + -0.2 6,025,252 + +0.4 6,025,252 + +0.7 6,025,252 + -0.6 6,025,252 + -3.1 6,025,252 + +1.1 6,025,252 + -2.1 6,025,252 + -1.3 6,025,252 + +0.3 6,025,252 + +0.4 6,025,252 + -2.6 6,025,253 - -1.1 6,025,253 - -0.9 6,025,253 - -1.1 6,025,253 - +0.5 6,025,253 - -1.0 6,025,253 - -0.7 6,025,253 - +0.5 6,025,253 - +0.6 6,025,253 - +1.0 6,025,264 + -0.1 6,025,281 - +1.6 6,025,439 + -0.6 6,025,701 + Pf6N2E2_5228 0.27 +1.2 6,025,887 + Pf6N2E2_5228 0.40 +1.0 6,026,029 + Pf6N2E2_5228 0.50 +0.7 6,026,084 - Pf6N2E2_5228 0.54 -1.6 6,026,285 - Pf6N2E2_5228 0.68 +1.8 6,026,285 - Pf6N2E2_5228 0.68 +0.8 6,026,285 - Pf6N2E2_5228 0.68 -0.1 6,026,320 + Pf6N2E2_5228 0.70 +0.5 6,026,320 + Pf6N2E2_5228 0.70 -1.7 6,026,321 - Pf6N2E2_5228 0.70 +0.4 6,026,321 - Pf6N2E2_5228 0.70 +0.9 6,026,505 + Pf6N2E2_5228 0.83 +1.0 6,026,662 + -2.0 6,026,662 + +0.5 6,026,663 - +1.0 6,026,718 + +1.1 6,026,718 + -0.5 6,026,718 + +0.4 6,026,719 - +1.1 6,026,767 + +2.0 6,026,767 + +1.0 6,026,767 + -0.3 6,026,767 + +1.7 6,026,767 + +0.6 6,026,767 + +0.2 6,026,768 - +0.3 6,026,768 - -0.2 6,026,768 - -0.8 6,026,768 - +0.8 6,026,768 - +2.6 6,026,768 - +0.3 6,026,768 - -0.5 6,026,768 - -0.1 6,026,959 + Pf6N2E2_5229 0.20 +0.3 6,027,081 + Pf6N2E2_5229 0.33 +0.5 6,027,081 + Pf6N2E2_5229 0.33 -1.6 6,027,081 + Pf6N2E2_5229 0.33 +1.5 6,027,081 + Pf6N2E2_5229 0.33 +0.8 6,027,082 - Pf6N2E2_5229 0.33 +0.4 6,027,082 - Pf6N2E2_5229 0.33 +0.2 6,027,082 - Pf6N2E2_5229 0.33 +1.4 6,027,082 - Pf6N2E2_5229 0.33 +0.7 6,027,082 - Pf6N2E2_5229 0.33 +0.2 6,027,157 + Pf6N2E2_5229 0.41 +0.8 6,027,157 + Pf6N2E2_5229 0.41 +1.7 6,027,157 + Pf6N2E2_5229 0.41 -1.5 6,027,158 - -0.6 6,027,158 - Pf6N2E2_5229 0.41 -2.2 6,027,158 - Pf6N2E2_5229 0.41 +0.7 6,027,193 + Pf6N2E2_5229 0.45 -0.2 6,027,194 - Pf6N2E2_5229 0.45 +2.0 6,027,299 - Pf6N2E2_5229 0.56 +0.3 6,027,630 + +0.6 6,027,630 + -0.0 6,027,630 + -0.1 6,027,630 + -0.9 6,027,631 - -1.6 6,027,632 + -0.7 6,027,633 - +1.6 6,027,719 - -0.3 6,027,719 - -0.3
Or see this region's nucleotide sequence