Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4971 and Pf6N2E2_4972 are separated by 195 nucleotides Pf6N2E2_4972 and Pf6N2E2_4973 are separated by 111 nucleotides Pf6N2E2_4973 and Pf6N2E2_4974 are separated by 6 nucleotides
Pf6N2E2_4971 - Transcriptional regulator, at 5,736,619 to 5,737,350
_4971
Pf6N2E2_4972 - Inorganic pyrophosphatase (EC 3.6.1.1), at 5,737,546 to 5,738,073
_4972
Pf6N2E2_4973 - Inner membrane protein, at 5,738,185 to 5,738,997
_4973
Pf6N2E2_4974 - DedA protein, at 5,739,004 to 5,739,651
_4974
Position (kb)
5737
5738
5739 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5736.570 kb on + strand at 5736.570 kb on + strand at 5736.571 kb on - strand at 5736.571 kb on - strand at 5736.620 kb on + strand at 5736.621 kb on - strand at 5736.685 kb on + strand at 5736.686 kb on - strand at 5736.686 kb on - strand at 5736.686 kb on - strand at 5736.788 kb on + strand, within Pf6N2E2_4971 at 5736.788 kb on + strand, within Pf6N2E2_4971 at 5736.788 kb on + strand, within Pf6N2E2_4971 at 5736.789 kb on - strand, within Pf6N2E2_4971 at 5736.789 kb on - strand, within Pf6N2E2_4971 at 5736.824 kb on + strand, within Pf6N2E2_4971 at 5736.824 kb on + strand, within Pf6N2E2_4971 at 5736.943 kb on + strand, within Pf6N2E2_4971 at 5736.943 kb on + strand, within Pf6N2E2_4971 at 5736.944 kb on - strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.046 kb on + strand, within Pf6N2E2_4971 at 5737.047 kb on - strand, within Pf6N2E2_4971 at 5737.047 kb on - strand, within Pf6N2E2_4971 at 5737.085 kb on + strand, within Pf6N2E2_4971 at 5737.086 kb on - strand, within Pf6N2E2_4971 at 5737.086 kb on - strand, within Pf6N2E2_4971 at 5737.112 kb on + strand, within Pf6N2E2_4971 at 5737.112 kb on + strand, within Pf6N2E2_4971 at 5737.112 kb on + strand, within Pf6N2E2_4971 at 5737.112 kb on + strand, within Pf6N2E2_4971 at 5737.113 kb on - strand, within Pf6N2E2_4971 at 5737.113 kb on - strand, within Pf6N2E2_4971 at 5737.246 kb on + strand, within Pf6N2E2_4971 at 5737.247 kb on - strand, within Pf6N2E2_4971 at 5737.247 kb on - strand, within Pf6N2E2_4971 at 5737.268 kb on + strand, within Pf6N2E2_4971 at 5737.268 kb on + strand, within Pf6N2E2_4971 at 5737.269 kb on - strand, within Pf6N2E2_4971 at 5737.310 kb on - strand at 5737.387 kb on + strand at 5737.407 kb on - strand at 5737.408 kb on + strand at 5737.408 kb on + strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.409 kb on - strand at 5737.495 kb on + strand at 5737.502 kb on + strand at 5738.139 kb on - strand at 5738.139 kb on - strand at 5738.139 kb on - strand at 5738.145 kb on - strand at 5738.145 kb on - strand at 5738.178 kb on - strand at 5738.186 kb on + strand at 5738.186 kb on + strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.187 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.190 kb on - strand at 5738.202 kb on - strand at 5738.256 kb on - strand at 5738.276 kb on + strand, within Pf6N2E2_4973 at 5738.276 kb on + strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.277 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.325 kb on - strand, within Pf6N2E2_4973 at 5738.370 kb on - strand, within Pf6N2E2_4973 at 5738.552 kb on + strand, within Pf6N2E2_4973 at 5738.552 kb on + strand, within Pf6N2E2_4973 at 5738.729 kb on + strand, within Pf6N2E2_4973
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 5,736,570 + +1.4 5,736,570 + +0.0 5,736,571 - -1.5 5,736,571 - -0.2 5,736,620 + -0.2 5,736,621 - +0.4 5,736,685 + -1.2 5,736,686 - +3.0 5,736,686 - +0.1 5,736,686 - -1.8 5,736,788 + Pf6N2E2_4971 0.23 +0.1 5,736,788 + Pf6N2E2_4971 0.23 +0.8 5,736,788 + Pf6N2E2_4971 0.23 +0.4 5,736,789 - Pf6N2E2_4971 0.23 +0.9 5,736,789 - Pf6N2E2_4971 0.23 +0.0 5,736,824 + Pf6N2E2_4971 0.28 -1.2 5,736,824 + Pf6N2E2_4971 0.28 +0.3 5,736,943 + Pf6N2E2_4971 0.44 -1.2 5,736,943 + Pf6N2E2_4971 0.44 -0.2 5,736,944 - Pf6N2E2_4971 0.44 -0.6 5,737,046 + Pf6N2E2_4971 0.58 -0.3 5,737,046 + Pf6N2E2_4971 0.58 +0.0 5,737,046 + Pf6N2E2_4971 0.58 +0.2 5,737,046 + Pf6N2E2_4971 0.58 +0.3 5,737,046 + Pf6N2E2_4971 0.58 -0.9 5,737,046 + Pf6N2E2_4971 0.58 +0.7 5,737,046 + Pf6N2E2_4971 0.58 -0.3 5,737,047 - Pf6N2E2_4971 0.58 -0.7 5,737,047 - Pf6N2E2_4971 0.58 +1.4 5,737,085 + Pf6N2E2_4971 0.64 -1.0 5,737,086 - Pf6N2E2_4971 0.64 -1.2 5,737,086 - Pf6N2E2_4971 0.64 -0.4 5,737,112 + Pf6N2E2_4971 0.67 -0.6 5,737,112 + Pf6N2E2_4971 0.67 +0.0 5,737,112 + Pf6N2E2_4971 0.67 -1.2 5,737,112 + Pf6N2E2_4971 0.67 +0.7 5,737,113 - Pf6N2E2_4971 0.67 -2.2 5,737,113 - Pf6N2E2_4971 0.67 +0.9 5,737,246 + Pf6N2E2_4971 0.86 -0.8 5,737,247 - Pf6N2E2_4971 0.86 +1.0 5,737,247 - Pf6N2E2_4971 0.86 +0.2 5,737,268 + Pf6N2E2_4971 0.89 -0.8 5,737,268 + Pf6N2E2_4971 0.89 +0.7 5,737,269 - Pf6N2E2_4971 0.89 -0.8 5,737,310 - +0.8 5,737,387 + +0.7 5,737,407 - +2.8 5,737,408 + +0.4 5,737,408 + +0.6 5,737,409 - +0.0 5,737,409 - -0.5 5,737,409 - +0.2 5,737,409 - -2.5 5,737,495 + +0.3 5,737,502 + -0.5 5,738,139 - -0.0 5,738,139 - -0.3 5,738,139 - -0.8 5,738,145 - +1.0 5,738,145 - -0.8 5,738,178 - +0.4 5,738,186 + +0.4 5,738,186 + -0.5 5,738,187 - -0.9 5,738,187 - +0.5 5,738,187 - -0.7 5,738,187 - +0.1 5,738,187 - -1.0 5,738,187 - +0.9 5,738,190 - +0.7 5,738,190 - +0.0 5,738,190 - +1.7 5,738,190 - +0.8 5,738,190 - +0.1 5,738,190 - -0.8 5,738,202 - +1.3 5,738,256 - +0.4 5,738,276 + Pf6N2E2_4973 0.11 +0.7 5,738,276 + Pf6N2E2_4973 0.11 -0.5 5,738,277 - Pf6N2E2_4973 0.11 +0.7 5,738,277 - Pf6N2E2_4973 0.11 -1.1 5,738,277 - Pf6N2E2_4973 0.11 -1.7 5,738,277 - Pf6N2E2_4973 0.11 +0.0 5,738,325 - Pf6N2E2_4973 0.17 +1.1 5,738,325 - Pf6N2E2_4973 0.17 +0.1 5,738,325 - Pf6N2E2_4973 0.17 -3.3 5,738,325 - Pf6N2E2_4973 0.17 +0.2 5,738,370 - Pf6N2E2_4973 0.23 +0.4 5,738,552 + Pf6N2E2_4973 0.45 +0.5 5,738,552 + Pf6N2E2_4973 0.45 +1.7 5,738,729 + Pf6N2E2_4973 0.67 -2.0
Or see this region's nucleotide sequence