Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_395 and Pf6N2E2_396 are separated by 684 nucleotides Pf6N2E2_396 and Pf6N2E2_397 are separated by 162 nucleotides
Pf6N2E2_395 - Glutathione S-transferase (EC 2.5.1.18), at 463,397 to 463,828
_395
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Pf6N2E2_397 - Large exoproteins involved in heme utilization or adhesion, at 465,083 to 467,020
_397
Position (kb)
464
465 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 463.558 kb on + strand, within Pf6N2E2_395 at 463.602 kb on + strand, within Pf6N2E2_395 at 463.603 kb on - strand, within Pf6N2E2_395 at 463.697 kb on + strand, within Pf6N2E2_395 at 463.698 kb on - strand, within Pf6N2E2_395 at 463.731 kb on - strand, within Pf6N2E2_395 at 463.795 kb on - strand at 463.795 kb on - strand at 463.882 kb on - strand at 463.924 kb on - strand at 463.957 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.846 kb on + strand, within Pf6N2E2_396 at 464.857 kb on + strand, within Pf6N2E2_396 at 464.900 kb on + strand at 464.900 kb on + strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.901 kb on - strand at 464.913 kb on + strand at 464.913 kb on + strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.914 kb on - strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.918 kb on + strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.919 kb on - strand at 464.930 kb on + strand at 465.034 kb on + strand at 465.035 kb on - strand at 465.084 kb on + strand at 465.084 kb on + strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.085 kb on - strand at 465.225 kb on + strand at 465.225 kb on + strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.226 kb on - strand at 465.348 kb on + strand, within Pf6N2E2_397 at 465.391 kb on - strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.404 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.446 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.473 kb on + strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.474 kb on - strand, within Pf6N2E2_397 at 465.551 kb on + strand, within Pf6N2E2_397 at 465.552 kb on - strand, within Pf6N2E2_397 at 465.691 kb on - strand, within Pf6N2E2_397 at 465.820 kb on + strand, within Pf6N2E2_397 at 465.843 kb on + strand, within Pf6N2E2_397 at 465.874 kb on + strand, within Pf6N2E2_397 at 465.875 kb on - strand, within Pf6N2E2_397
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 463,558 + Pf6N2E2_395 0.37 -0.6 463,602 + Pf6N2E2_395 0.47 +0.8 463,603 - Pf6N2E2_395 0.48 -1.7 463,697 + Pf6N2E2_395 0.69 +0.9 463,698 - Pf6N2E2_395 0.70 -0.5 463,731 - Pf6N2E2_395 0.77 +3.2 463,795 - +0.3 463,795 - -0.6 463,882 - +0.4 463,924 - -2.4 463,957 - -1.2 464,009 - -0.6 464,009 - +1.7 464,475 + -0.6 464,476 - -1.3 464,508 - +0.4 464,508 - -0.6 464,508 - -0.5 464,508 - -2.2 464,511 + +0.2 464,511 + -2.2 464,511 + +0.4 464,511 + +0.0 464,512 - +2.3 464,512 - +0.4 464,532 + +1.4 464,532 + +1.2 464,533 - -0.4 464,533 - +1.8 464,594 - Pf6N2E2_396 0.20 -1.6 464,594 - Pf6N2E2_396 0.20 -2.9 464,594 - Pf6N2E2_396 0.20 -1.6 464,690 + Pf6N2E2_396 0.43 -1.2 464,719 - Pf6N2E2_396 0.50 +0.6 464,719 - Pf6N2E2_396 0.50 +0.4 464,812 + Pf6N2E2_396 0.73 -0.3 464,812 + Pf6N2E2_396 0.73 -2.2 464,812 + Pf6N2E2_396 0.73 -1.8 464,846 + Pf6N2E2_396 0.82 -1.0 464,857 + Pf6N2E2_396 0.84 -1.2 464,900 + -1.6 464,900 + +1.8 464,901 - +0.4 464,901 - -1.2 464,901 - +0.9 464,913 + -0.3 464,913 + +0.4 464,914 - -0.4 464,914 - -0.3 464,914 - +0.4 464,918 + +0.4 464,918 + -0.6 464,918 + -2.7 464,919 - -0.1 464,919 - -0.5 464,919 - -1.9 464,919 - +1.8 464,919 - +0.9 464,919 - +0.2 464,919 - +1.4 464,930 + +0.4 465,034 + +1.4 465,035 - +1.9 465,084 + -0.9 465,084 + +1.4 465,085 - -0.2 465,085 - +0.8 465,085 - +0.2 465,085 - +0.5 465,085 - -0.9 465,225 + +0.4 465,225 + +0.4 465,226 - -0.8 465,226 - +0.4 465,226 - +0.0 465,226 - -0.6 465,226 - +0.2 465,226 - -1.4 465,226 - -0.2 465,348 + Pf6N2E2_397 0.14 -1.0 465,391 - Pf6N2E2_397 0.16 -0.6 465,404 + Pf6N2E2_397 0.17 +0.8 465,404 + Pf6N2E2_397 0.17 +0.8 465,446 + Pf6N2E2_397 0.19 +0.1 465,446 + Pf6N2E2_397 0.19 -0.8 465,473 + Pf6N2E2_397 0.20 -1.2 465,473 + Pf6N2E2_397 0.20 -0.3 465,473 + Pf6N2E2_397 0.20 -1.0 465,473 + Pf6N2E2_397 0.20 +0.8 465,474 - Pf6N2E2_397 0.20 +0.4 465,474 - Pf6N2E2_397 0.20 -1.6 465,474 - Pf6N2E2_397 0.20 -0.5 465,474 - Pf6N2E2_397 0.20 +0.1 465,474 - Pf6N2E2_397 0.20 +0.4 465,474 - Pf6N2E2_397 0.20 +1.0 465,474 - Pf6N2E2_397 0.20 +0.4 465,551 + Pf6N2E2_397 0.24 +0.4 465,552 - Pf6N2E2_397 0.24 -1.2 465,691 - Pf6N2E2_397 0.31 -0.0 465,820 + Pf6N2E2_397 0.38 +1.4 465,843 + Pf6N2E2_397 0.39 -1.6 465,874 + Pf6N2E2_397 0.41 +0.4 465,875 - Pf6N2E2_397 0.41 +0.7
Or see this region's nucleotide sequence