Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2109 and Pf6N2E2_2110 are separated by 29 nucleotides Pf6N2E2_2110 and Pf6N2E2_2111 are separated by 168 nucleotides Pf6N2E2_2111 and Pf6N2E2_2112 are separated by 35 nucleotides
Pf6N2E2_2109 - NAD-dependent protein deacetylase of SIR2 family, at 2,506,171 to 2,507,010
_2109
Pf6N2E2_2110 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 2,507,040 to 2,507,942
_2110
Pf6N2E2_2111 - Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit A (from data) , at 2,508,111 to 2,508,809
_2111
Pf6N2E2_2112 - Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit B (from data) , at 2,508,845 to 2,509,510
_2112
Position (kb)
2507
2508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.855 kb on + strand, within Pf6N2E2_2109 at 2506.856 kb on - strand, within Pf6N2E2_2109 at 2506.856 kb on - strand, within Pf6N2E2_2109 at 2507.008 kb on + strand at 2507.008 kb on + strand at 2507.009 kb on - strand at 2507.009 kb on - strand at 2507.461 kb on + strand, within Pf6N2E2_2110 at 2507.461 kb on + strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.792 kb on + strand, within Pf6N2E2_2110 at 2507.792 kb on + strand, within Pf6N2E2_2110 at 2507.793 kb on - strand, within Pf6N2E2_2110 at 2507.972 kb on + strand at 2507.973 kb on - strand at 2508.097 kb on + strand at 2508.097 kb on + strand at 2508.098 kb on - strand at 2508.098 kb on - strand at 2508.142 kb on - strand at 2508.307 kb on + strand, within Pf6N2E2_2111 at 2508.308 kb on - strand, within Pf6N2E2_2111 at 2508.348 kb on + strand, within Pf6N2E2_2111 at 2508.348 kb on + strand at 2508.348 kb on + strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.744 kb on + strand at 2508.745 kb on - strand at 2508.839 kb on - strand at 2508.839 kb on - strand at 2508.902 kb on + strand at 2508.903 kb on - strand at 2508.903 kb on - strand at 2508.905 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 2,506,046 + -0.3 2,506,046 + +1.5 2,506,046 + +0.9 2,506,046 + +1.4 2,506,046 + +0.3 2,506,046 + +1.8 2,506,046 + +1.3 2,506,047 - -2.1 2,506,047 - -0.3 2,506,047 - +1.6 2,506,047 - +0.8 2,506,047 - +0.5 2,506,047 - -0.3 2,506,047 - -0.0 2,506,047 - -0.5 2,506,047 - -1.8 2,506,047 - -1.1 2,506,047 - -1.5 2,506,047 - -0.2 2,506,047 - +1.3 2,506,047 - +2.1 2,506,047 - -0.7 2,506,216 + -0.7 2,506,216 + -0.5 2,506,216 + -1.5 2,506,216 + -1.1 2,506,216 + +0.3 2,506,216 + +1.9 2,506,216 + +1.1 2,506,217 - -1.8 2,506,217 - +1.0 2,506,217 - -1.2 2,506,217 - +0.7 2,506,217 - -0.4 2,506,217 - +0.1 2,506,330 + Pf6N2E2_2109 0.19 -1.7 2,506,330 + Pf6N2E2_2109 0.19 -0.5 2,506,330 + Pf6N2E2_2109 0.19 +1.2 2,506,331 - Pf6N2E2_2109 0.19 -0.1 2,506,331 - Pf6N2E2_2109 0.19 +1.2 2,506,331 - Pf6N2E2_2109 0.19 +0.3 2,506,855 + Pf6N2E2_2109 0.81 +1.3 2,506,856 - Pf6N2E2_2109 0.82 +0.5 2,506,856 - Pf6N2E2_2109 0.82 -1.5 2,507,008 + +0.8 2,507,008 + +1.5 2,507,009 - +0.9 2,507,009 - +0.7 2,507,461 + Pf6N2E2_2110 0.47 -3.4 2,507,461 + Pf6N2E2_2110 0.47 +0.5 2,507,462 - Pf6N2E2_2110 0.47 -0.7 2,507,462 - Pf6N2E2_2110 0.47 -0.5 2,507,462 - Pf6N2E2_2110 0.47 -2.5 2,507,792 + Pf6N2E2_2110 0.83 -2.8 2,507,792 + Pf6N2E2_2110 0.83 +0.5 2,507,793 - Pf6N2E2_2110 0.83 -1.1 2,507,972 + -1.5 2,507,973 - -0.7 2,508,097 + +0.2 2,508,097 + +0.5 2,508,098 - -0.3 2,508,098 - +0.9 2,508,142 - +0.1 2,508,307 + Pf6N2E2_2111 0.28 -0.1 2,508,308 - Pf6N2E2_2111 0.28 -1.5 2,508,348 + Pf6N2E2_2111 0.34 +1.5 2,508,348 + -1.5 2,508,348 + Pf6N2E2_2111 0.34 -1.1 2,508,349 - Pf6N2E2_2111 0.34 +1.5 2,508,349 - Pf6N2E2_2111 0.34 -1.5 2,508,349 - Pf6N2E2_2111 0.34 -0.4 2,508,744 + -0.4 2,508,745 - +0.1 2,508,839 - +0.4 2,508,839 - -0.5 2,508,902 + +0.9 2,508,903 - -1.4 2,508,903 - +0.8 2,508,905 + -0.5
Or see this region's nucleotide sequence