Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1975 and Pf6N2E2_1976 are separated by 11 nucleotides Pf6N2E2_1976 and Pf6N2E2_1977 are separated by 216 nucleotides Pf6N2E2_1977 and Pf6N2E2_1978 are separated by 8 nucleotides Pf6N2E2_1978 and Pf6N2E2_1979 are separated by 461 nucleotides
Pf6N2E2_1975 - Integrase regulator R, at 2,341,340 to 2,341,840
_1975
Pf6N2E2_1976 - Single-stranded DNA-binding protein, at 2,341,852 to 2,342,256
_1976
Pf6N2E2_1977 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,342,473 to 2,344,023
_1977
Pf6N2E2_1978 - DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster, at 2,344,032 to 2,344,559
_1978
Pf6N2E2_1979 - Cytosine-specific DNA methyltransferase (EC 2.1.1.37), at 2,345,021 to 2,346,277
_1979
Position (kb)
2342
2343
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.805 kb on + strand at 2341.806 kb on - strand at 2341.836 kb on + strand at 2341.836 kb on + strand at 2341.837 kb on - strand at 2341.837 kb on - strand at 2341.879 kb on - strand at 2341.879 kb on - strand at 2341.882 kb on - strand at 2342.595 kb on - strand at 2342.695 kb on + strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.696 kb on - strand, within Pf6N2E2_1977 at 2342.808 kb on - strand, within Pf6N2E2_1977 at 2342.999 kb on - strand, within Pf6N2E2_1977 at 2343.090 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.131 kb on + strand, within Pf6N2E2_1977 at 2343.132 kb on - strand, within Pf6N2E2_1977 at 2343.138 kb on - strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.212 kb on + strand, within Pf6N2E2_1977 at 2343.342 kb on - strand, within Pf6N2E2_1977 at 2343.472 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.727 kb on + strand, within Pf6N2E2_1977 at 2343.748 kb on + strand, within Pf6N2E2_1977 at 2343.779 kb on - strand, within Pf6N2E2_1977 at 2343.818 kb on + strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.819 kb on - strand, within Pf6N2E2_1977 at 2343.972 kb on + strand at 2343.973 kb on - strand at 2344.005 kb on + strand at 2344.124 kb on + strand, within Pf6N2E2_1978 at 2344.152 kb on + strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.153 kb on - strand, within Pf6N2E2_1978 at 2344.158 kb on + strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.159 kb on - strand, within Pf6N2E2_1978 at 2344.659 kb on - strand at 2344.659 kb on - strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.749 kb on + strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand at 2344.750 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 2,341,727 - Pf6N2E2_1975 0.77 +0.5 2,341,727 - Pf6N2E2_1975 0.77 +0.6 2,341,805 + +0.5 2,341,806 - +0.4 2,341,836 + +0.5 2,341,836 + +0.5 2,341,837 - -0.5 2,341,837 - +0.4 2,341,879 - +0.8 2,341,879 - +0.3 2,341,882 - +0.1 2,342,595 - +1.0 2,342,695 + Pf6N2E2_1977 0.14 +0.9 2,342,696 - Pf6N2E2_1977 0.14 +0.3 2,342,696 - Pf6N2E2_1977 0.14 -2.3 2,342,808 - Pf6N2E2_1977 0.22 +0.5 2,342,999 - Pf6N2E2_1977 0.34 -0.7 2,343,090 + Pf6N2E2_1977 0.40 -0.5 2,343,131 + Pf6N2E2_1977 0.42 +1.2 2,343,131 + Pf6N2E2_1977 0.42 +0.2 2,343,132 - Pf6N2E2_1977 0.42 +1.0 2,343,138 - Pf6N2E2_1977 0.43 +0.0 2,343,212 + Pf6N2E2_1977 0.48 -0.2 2,343,212 + Pf6N2E2_1977 0.48 -1.3 2,343,342 - Pf6N2E2_1977 0.56 +2.1 2,343,472 + Pf6N2E2_1977 0.64 -0.5 2,343,727 + Pf6N2E2_1977 0.81 +0.3 2,343,727 + Pf6N2E2_1977 0.81 -0.5 2,343,748 + Pf6N2E2_1977 0.82 +1.3 2,343,779 - Pf6N2E2_1977 0.84 +0.5 2,343,818 + Pf6N2E2_1977 0.87 -2.7 2,343,819 - Pf6N2E2_1977 0.87 -1.8 2,343,819 - Pf6N2E2_1977 0.87 -1.1 2,343,972 + +1.8 2,343,973 - +0.0 2,344,005 + -1.2 2,344,124 + Pf6N2E2_1978 0.17 -0.1 2,344,152 + Pf6N2E2_1978 0.23 +0.7 2,344,153 - Pf6N2E2_1978 0.23 +1.6 2,344,153 - Pf6N2E2_1978 0.23 +1.9 2,344,158 + Pf6N2E2_1978 0.24 -0.5 2,344,159 - Pf6N2E2_1978 0.24 +0.4 2,344,159 - Pf6N2E2_1978 0.24 +0.1 2,344,159 - Pf6N2E2_1978 0.24 +0.5 2,344,159 - Pf6N2E2_1978 0.24 -0.5 2,344,159 - Pf6N2E2_1978 0.24 +0.5 2,344,659 - +0.1 2,344,659 - -0.5 2,344,749 + +0.1 2,344,749 + +0.2 2,344,749 + +1.6 2,344,750 - -0.3 2,344,750 - +0.2 2,344,750 - +0.5 2,344,750 - -0.2
Or see this region's nucleotide sequence