Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1734 and Pf6N2E2_1735 are separated by 23 nucleotides Pf6N2E2_1735 and Pf6N2E2_1736 are separated by 327 nucleotides Pf6N2E2_1736 and Pf6N2E2_1737 are separated by 331 nucleotides
Pf6N2E2_1734 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 2,046,985 to 2,047,392
_1734
Pf6N2E2_1735 - PhnB protein, at 2,047,416 to 2,047,841
_1735
Pf6N2E2_1736 - blr1018; hypothetical protein, at 2,048,169 to 2,049,419
_1736
Pf6N2E2_1737 - hypothetical protein, at 2,049,751 to 2,050,389
_1737
Position (kb)
2048
2049
2050 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2047.324 kb on + strand, within Pf6N2E2_1734 at 2047.332 kb on + strand, within Pf6N2E2_1734 at 2047.333 kb on - strand, within Pf6N2E2_1734 at 2047.333 kb on - strand, within Pf6N2E2_1734 at 2047.335 kb on - strand, within Pf6N2E2_1734 at 2047.335 kb on - strand, within Pf6N2E2_1734 at 2047.360 kb on - strand at 2047.377 kb on - strand at 2047.395 kb on + strand at 2047.396 kb on - strand at 2047.437 kb on + strand at 2047.753 kb on + strand, within Pf6N2E2_1735 at 2047.754 kb on - strand, within Pf6N2E2_1735 at 2047.754 kb on - strand, within Pf6N2E2_1735 at 2047.898 kb on + strand at 2047.939 kb on + strand at 2047.945 kb on + strand at 2047.946 kb on - strand at 2047.946 kb on - strand at 2048.022 kb on + strand at 2048.022 kb on + strand at 2048.023 kb on - strand at 2048.099 kb on - strand at 2048.139 kb on - strand at 2048.250 kb on + strand at 2048.250 kb on + strand at 2048.251 kb on - strand at 2048.265 kb on + strand at 2048.266 kb on - strand at 2048.266 kb on - strand at 2048.266 kb on - strand at 2048.267 kb on + strand at 2048.268 kb on - strand at 2048.268 kb on - strand at 2048.376 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.464 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.475 kb on + strand, within Pf6N2E2_1736 at 2048.476 kb on - strand, within Pf6N2E2_1736 at 2048.553 kb on + strand, within Pf6N2E2_1736 at 2048.554 kb on - strand, within Pf6N2E2_1736 at 2048.589 kb on + strand, within Pf6N2E2_1736 at 2048.913 kb on + strand, within Pf6N2E2_1736 at 2048.997 kb on + strand, within Pf6N2E2_1736 at 2048.998 kb on - strand, within Pf6N2E2_1736 at 2049.256 kb on - strand, within Pf6N2E2_1736 at 2049.256 kb on - strand, within Pf6N2E2_1736 at 2049.286 kb on + strand, within Pf6N2E2_1736 at 2049.376 kb on - strand at 2049.462 kb on + strand at 2049.463 kb on - strand at 2049.504 kb on - strand at 2049.554 kb on - strand at 2049.554 kb on - strand at 2049.581 kb on - strand at 2049.591 kb on + strand at 2049.592 kb on - strand at 2049.883 kb on + strand, within Pf6N2E2_1737 at 2049.883 kb on + strand, within Pf6N2E2_1737 at 2049.883 kb on + strand, within Pf6N2E2_1737 at 2049.883 kb on + strand, within Pf6N2E2_1737 at 2049.884 kb on - strand, within Pf6N2E2_1737 at 2049.884 kb on - strand, within Pf6N2E2_1737 at 2049.884 kb on - strand, within Pf6N2E2_1737 at 2049.884 kb on - strand, within Pf6N2E2_1737 at 2049.885 kb on + strand, within Pf6N2E2_1737 at 2049.936 kb on + strand, within Pf6N2E2_1737 at 2050.037 kb on + strand, within Pf6N2E2_1737 at 2050.037 kb on + strand, within Pf6N2E2_1737 at 2050.037 kb on + strand, within Pf6N2E2_1737 at 2050.171 kb on + strand, within Pf6N2E2_1737 at 2050.171 kb on + strand, within Pf6N2E2_1737 at 2050.215 kb on + strand, within Pf6N2E2_1737
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 2,047,324 + Pf6N2E2_1734 0.83 +0.2 2,047,332 + Pf6N2E2_1734 0.85 +0.9 2,047,333 - Pf6N2E2_1734 0.85 -2.5 2,047,333 - Pf6N2E2_1734 0.85 +0.3 2,047,335 - Pf6N2E2_1734 0.86 +0.3 2,047,335 - Pf6N2E2_1734 0.86 +1.1 2,047,360 - +0.3 2,047,377 - -2.7 2,047,395 + +0.3 2,047,396 - -0.5 2,047,437 + +2.1 2,047,753 + Pf6N2E2_1735 0.79 -0.3 2,047,754 - Pf6N2E2_1735 0.79 +0.7 2,047,754 - Pf6N2E2_1735 0.79 +1.1 2,047,898 + +0.1 2,047,939 + -1.3 2,047,945 + -0.8 2,047,946 - +0.1 2,047,946 - +0.7 2,048,022 + -1.3 2,048,022 + +1.1 2,048,023 - +1.3 2,048,099 - -0.7 2,048,139 - +0.6 2,048,250 + -0.1 2,048,250 + +0.7 2,048,251 - -0.5 2,048,265 + +1.1 2,048,266 - -1.3 2,048,266 - -2.3 2,048,266 - -0.1 2,048,267 + +1.4 2,048,268 - +0.7 2,048,268 - -0.7 2,048,376 + Pf6N2E2_1736 0.17 -0.7 2,048,464 + Pf6N2E2_1736 0.24 +1.1 2,048,464 + Pf6N2E2_1736 0.24 +0.0 2,048,464 + Pf6N2E2_1736 0.24 +0.6 2,048,464 + Pf6N2E2_1736 0.24 +1.0 2,048,475 + Pf6N2E2_1736 0.24 +0.5 2,048,475 + Pf6N2E2_1736 0.24 -0.8 2,048,475 + Pf6N2E2_1736 0.24 +0.9 2,048,475 + Pf6N2E2_1736 0.24 -0.9 2,048,475 + Pf6N2E2_1736 0.24 +1.7 2,048,476 - Pf6N2E2_1736 0.25 +3.7 2,048,553 + Pf6N2E2_1736 0.31 +1.3 2,048,554 - Pf6N2E2_1736 0.31 +0.4 2,048,589 + Pf6N2E2_1736 0.34 -2.7 2,048,913 + Pf6N2E2_1736 0.59 +0.8 2,048,997 + Pf6N2E2_1736 0.66 -1.0 2,048,998 - Pf6N2E2_1736 0.66 -1.3 2,049,256 - Pf6N2E2_1736 0.87 +1.3 2,049,256 - Pf6N2E2_1736 0.87 +2.0 2,049,286 + Pf6N2E2_1736 0.89 +1.3 2,049,376 - +0.7 2,049,462 + +0.4 2,049,463 - -0.5 2,049,504 - -2.7 2,049,554 - +1.9 2,049,554 - -1.0 2,049,581 - -0.3 2,049,591 + -0.7 2,049,592 - +0.3 2,049,883 + Pf6N2E2_1737 0.21 -2.0 2,049,883 + Pf6N2E2_1737 0.21 -1.1 2,049,883 + Pf6N2E2_1737 0.21 +0.3 2,049,883 + Pf6N2E2_1737 0.21 -0.4 2,049,884 - Pf6N2E2_1737 0.21 +0.6 2,049,884 - Pf6N2E2_1737 0.21 +0.6 2,049,884 - Pf6N2E2_1737 0.21 -1.7 2,049,884 - Pf6N2E2_1737 0.21 +0.1 2,049,885 + Pf6N2E2_1737 0.21 +1.0 2,049,936 + Pf6N2E2_1737 0.29 +0.9 2,050,037 + Pf6N2E2_1737 0.45 +0.7 2,050,037 + Pf6N2E2_1737 0.45 +0.3 2,050,037 + Pf6N2E2_1737 0.45 +1.5 2,050,171 + Pf6N2E2_1737 0.66 -0.7 2,050,171 + Pf6N2E2_1737 0.66 -0.4 2,050,215 + Pf6N2E2_1737 0.73 +0.6
Or see this region's nucleotide sequence