Experiment: RCH2 defined with Cobalt chloride 0.02 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1225 and Pf6N2E2_1226 overlap by 4 nucleotides Pf6N2E2_1226 and Pf6N2E2_1227 are separated by 220 nucleotides Pf6N2E2_1227 and Pf6N2E2_1228 are separated by 128 nucleotides
Pf6N2E2_1225 - DinG family ATP-dependent helicase CPE1197, at 1,432,159 to 1,434,471
_1225
Pf6N2E2_1226 - DNA damage response nuclease (from data) , at 1,434,468 to 1,436,138
_1226
Pf6N2E2_1227 - FIG00955097: hypothetical protein, at 1,436,359 to 1,436,583
_1227
Pf6N2E2_1228 - Lipase (EC 3.1.1.3), at 1,436,712 to 1,438,565
_1228
Position (kb)
1434
1435
1436
1437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.579 kb on + strand, within Pf6N2E2_1225 at 1433.580 kb on - strand, within Pf6N2E2_1225 at 1433.701 kb on + strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.764 kb on - strand, within Pf6N2E2_1225 at 1433.998 kb on + strand, within Pf6N2E2_1225 at 1433.999 kb on - strand, within Pf6N2E2_1225 at 1434.054 kb on + strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.515 kb on - strand at 1434.515 kb on - strand at 1434.774 kb on + strand, within Pf6N2E2_1226 at 1434.852 kb on + strand, within Pf6N2E2_1226 at 1434.853 kb on - strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.325 kb on - strand, within Pf6N2E2_1226 at 1435.570 kb on + strand, within Pf6N2E2_1226 at 1435.679 kb on - strand, within Pf6N2E2_1226 at 1435.679 kb on - strand, within Pf6N2E2_1226 at 1436.104 kb on + strand at 1436.104 kb on + strand at 1436.107 kb on + strand at 1436.107 kb on + strand at 1436.108 kb on - strand at 1436.108 kb on - strand at 1436.191 kb on + strand at 1436.229 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.235 kb on + strand at 1436.235 kb on + strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.352 kb on + strand at 1436.461 kb on + strand, within Pf6N2E2_1227 at 1436.652 kb on + strand at 1436.652 kb on + strand at 1436.653 kb on - strand at 1436.658 kb on + strand at 1436.659 kb on - strand at 1436.665 kb on + strand at 1436.665 kb on + strand at 1436.666 kb on - strand at 1436.821 kb on + strand at 1436.821 kb on + strand at 1436.822 kb on - strand at 1436.830 kb on + strand at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Cobalt chloride 0.02 mM remove 1,433,477 + Pf6N2E2_1225 0.57 -0.9 1,433,477 + Pf6N2E2_1225 0.57 +0.2 1,433,477 + Pf6N2E2_1225 0.57 +0.6 1,433,481 - Pf6N2E2_1225 0.57 +0.2 1,433,481 - Pf6N2E2_1225 0.57 +0.2 1,433,579 + Pf6N2E2_1225 0.61 -0.3 1,433,580 - Pf6N2E2_1225 0.61 +0.8 1,433,701 + Pf6N2E2_1225 0.67 +0.3 1,433,702 - Pf6N2E2_1225 0.67 +1.2 1,433,702 - Pf6N2E2_1225 0.67 +0.6 1,433,764 - Pf6N2E2_1225 0.69 +1.2 1,433,998 + Pf6N2E2_1225 0.80 -0.2 1,433,999 - Pf6N2E2_1225 0.80 +0.9 1,434,054 + Pf6N2E2_1225 0.82 +1.2 1,434,122 - Pf6N2E2_1225 0.85 +0.5 1,434,122 - Pf6N2E2_1225 0.85 +0.2 1,434,514 + +3.1 1,434,514 + -0.8 1,434,514 + +0.5 1,434,515 - -0.5 1,434,515 - +0.5 1,434,774 + Pf6N2E2_1226 0.18 +1.8 1,434,852 + Pf6N2E2_1226 0.23 +2.2 1,434,853 - Pf6N2E2_1226 0.23 +1.4 1,435,018 + Pf6N2E2_1226 0.33 +0.3 1,435,018 + Pf6N2E2_1226 0.33 -0.3 1,435,018 + Pf6N2E2_1226 0.33 -1.2 1,435,018 + Pf6N2E2_1226 0.33 +0.7 1,435,018 + Pf6N2E2_1226 0.33 -1.4 1,435,019 - Pf6N2E2_1226 0.33 +1.2 1,435,019 - Pf6N2E2_1226 0.33 -0.8 1,435,324 + Pf6N2E2_1226 0.51 -0.6 1,435,324 + Pf6N2E2_1226 0.51 +0.8 1,435,325 - Pf6N2E2_1226 0.51 +2.3 1,435,570 + Pf6N2E2_1226 0.66 +1.8 1,435,679 - Pf6N2E2_1226 0.72 -1.4 1,435,679 - Pf6N2E2_1226 0.72 -0.8 1,436,104 + -0.4 1,436,104 + +1.1 1,436,107 + -0.0 1,436,107 + -2.2 1,436,108 - +0.9 1,436,108 - -0.0 1,436,191 + +0.2 1,436,229 + +0.8 1,436,231 + +1.4 1,436,231 + +0.2 1,436,231 + -0.8 1,436,232 - +1.6 1,436,232 - +0.2 1,436,232 - +0.2 1,436,235 + -1.3 1,436,235 + +1.2 1,436,236 - +0.2 1,436,236 - -1.8 1,436,236 - -2.4 1,436,352 + +0.8 1,436,461 + Pf6N2E2_1227 0.45 -0.8 1,436,652 + -0.8 1,436,652 + -0.8 1,436,653 - -2.8 1,436,658 + +0.2 1,436,659 - -0.8 1,436,665 + -0.8 1,436,665 + +0.6 1,436,666 - +2.5 1,436,821 + -1.2 1,436,821 + -0.8 1,436,822 - +0.1 1,436,830 + -0.2 1,437,106 + Pf6N2E2_1228 0.21 +1.2 1,437,106 + Pf6N2E2_1228 0.21 -2.4 1,437,106 + Pf6N2E2_1228 0.21 +1.4 1,437,107 - Pf6N2E2_1228 0.21 -0.7 1,437,107 - Pf6N2E2_1228 0.21 +1.4 1,437,107 - Pf6N2E2_1228 0.21 -0.2
Or see this region's nucleotide sequence