Experiment: KB with Cimicifugoside H1 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2364 and Psyr_2365 overlap by 4 nucleotides Psyr_2365 and Psyr_2366 are separated by 356 nucleotides
Psyr_2364: Psyr_2364 - conserved hypothetical protein, at 2,741,814 to 2,742,653
_2364
Psyr_2365: Psyr_2365 - Radical SAM, at 2,742,650 to 2,743,870
_2365
Psyr_2366: Psyr_2366 - ABC transporter, at 2,744,227 to 2,744,973
_2366
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2741.677 kb on + strand at 2741.677 kb on + strand at 2741.698 kb on - strand at 2741.698 kb on - strand at 2741.716 kb on + strand at 2741.801 kb on + strand at 2741.801 kb on + strand at 2741.801 kb on + strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2741.802 kb on - strand at 2742.010 kb on + strand, within Psyr_2364 at 2742.010 kb on + strand, within Psyr_2364 at 2742.013 kb on + strand, within Psyr_2364 at 2742.014 kb on - strand, within Psyr_2364 at 2742.112 kb on + strand, within Psyr_2364 at 2742.113 kb on - strand, within Psyr_2364 at 2742.115 kb on + strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.116 kb on - strand, within Psyr_2364 at 2742.226 kb on + strand, within Psyr_2364 at 2742.409 kb on + strand, within Psyr_2364 at 2743.015 kb on - strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.041 kb on + strand, within Psyr_2365 at 2743.042 kb on - strand, within Psyr_2365 at 2743.042 kb on - strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.122 kb on + strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.123 kb on - strand, within Psyr_2365 at 2743.125 kb on + strand, within Psyr_2365 at 2743.126 kb on - strand, within Psyr_2365 at 2743.152 kb on + strand, within Psyr_2365 at 2743.152 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.284 kb on + strand, within Psyr_2365 at 2743.452 kb on + strand, within Psyr_2365 at 2743.453 kb on - strand, within Psyr_2365 at 2743.516 kb on - strand, within Psyr_2365 at 2743.575 kb on + strand, within Psyr_2365 at 2743.657 kb on - strand, within Psyr_2365 at 2743.726 kb on + strand, within Psyr_2365 at 2743.726 kb on + strand, within Psyr_2365 at 2743.727 kb on - strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.728 kb on + strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.729 kb on - strand, within Psyr_2365 at 2743.805 kb on - strand at 2743.824 kb on + strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.825 kb on - strand at 2743.861 kb on + strand at 2743.892 kb on + strand at 2743.893 kb on - strand at 2743.981 kb on + strand at 2743.981 kb on + strand at 2743.982 kb on - strand at 2744.076 kb on - strand at 2744.076 kb on - strand at 2744.081 kb on + strand at 2744.188 kb on - strand at 2744.285 kb on + strand at 2744.285 kb on + strand at 2744.286 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cimicifugoside H1 10 mM remove 2,741,677 + +1.1 2,741,677 + -0.6 2,741,698 - -2.3 2,741,698 - -0.6 2,741,716 + +1.0 2,741,801 + +0.8 2,741,801 + -0.2 2,741,801 + +0.1 2,741,802 - -2.1 2,741,802 - +0.1 2,741,802 - -3.1 2,741,802 - -1.7 2,742,010 + Psyr_2364 0.23 -0.0 2,742,010 + Psyr_2364 0.23 +0.0 2,742,013 + Psyr_2364 0.24 +0.3 2,742,014 - Psyr_2364 0.24 -1.0 2,742,112 + Psyr_2364 0.35 -0.0 2,742,113 - Psyr_2364 0.36 +1.7 2,742,115 + Psyr_2364 0.36 +0.3 2,742,116 - Psyr_2364 0.36 -0.1 2,742,116 - Psyr_2364 0.36 -1.4 2,742,116 - Psyr_2364 0.36 -0.0 2,742,226 + Psyr_2364 0.49 +1.0 2,742,409 + Psyr_2364 0.71 +0.2 2,743,015 - Psyr_2365 0.30 +0.4 2,743,041 + Psyr_2365 0.32 +1.3 2,743,041 + Psyr_2365 0.32 +0.8 2,743,041 + Psyr_2365 0.32 -1.9 2,743,041 + Psyr_2365 0.32 +1.5 2,743,042 - Psyr_2365 0.32 -0.5 2,743,042 - Psyr_2365 0.32 +1.4 2,743,122 + Psyr_2365 0.39 +0.5 2,743,122 + Psyr_2365 0.39 +1.5 2,743,122 + Psyr_2365 0.39 +0.9 2,743,122 + Psyr_2365 0.39 +1.2 2,743,123 - Psyr_2365 0.39 -0.0 2,743,123 - Psyr_2365 0.39 -0.2 2,743,123 - Psyr_2365 0.39 -0.5 2,743,123 - Psyr_2365 0.39 +0.8 2,743,125 + Psyr_2365 0.39 -0.1 2,743,126 - Psyr_2365 0.39 +0.8 2,743,152 + Psyr_2365 0.41 +0.3 2,743,152 + Psyr_2365 0.41 +0.7 2,743,284 + Psyr_2365 0.52 +0.7 2,743,284 + Psyr_2365 0.52 +0.7 2,743,284 + Psyr_2365 0.52 +0.2 2,743,452 + Psyr_2365 0.66 +0.7 2,743,453 - Psyr_2365 0.66 -0.3 2,743,516 - Psyr_2365 0.71 +1.2 2,743,575 + Psyr_2365 0.76 -0.1 2,743,657 - Psyr_2365 0.82 +0.9 2,743,726 + Psyr_2365 0.88 +0.5 2,743,726 + Psyr_2365 0.88 -0.9 2,743,727 - Psyr_2365 0.88 -1.2 2,743,728 + Psyr_2365 0.88 -0.7 2,743,728 + Psyr_2365 0.88 -0.7 2,743,728 + Psyr_2365 0.88 -2.2 2,743,728 + Psyr_2365 0.88 +1.2 2,743,728 + Psyr_2365 0.88 +0.8 2,743,728 + Psyr_2365 0.88 +0.2 2,743,729 - Psyr_2365 0.88 -0.6 2,743,729 - Psyr_2365 0.88 +2.4 2,743,729 - Psyr_2365 0.88 -1.1 2,743,729 - Psyr_2365 0.88 -0.8 2,743,729 - Psyr_2365 0.88 -1.7 2,743,729 - Psyr_2365 0.88 -0.3 2,743,805 - +0.1 2,743,824 + -1.7 2,743,825 - -0.7 2,743,825 - -1.2 2,743,825 - -1.7 2,743,825 - -0.7 2,743,861 + +0.4 2,743,892 + -0.1 2,743,893 - -1.0 2,743,981 + +1.1 2,743,981 + +0.2 2,743,982 - +0.5 2,744,076 - +1.5 2,744,076 - -1.0 2,744,081 + +0.1 2,744,188 - +0.0 2,744,285 + -1.1 2,744,285 + -0.9 2,744,286 - +0.6
Or see this region's nucleotide sequence