Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0258

Experiment: KB with Cimicifugoside H1 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0257 and Psyr_0258 are separated by 299 nucleotidesPsyr_0258 and Psyr_0259 are separated by 151 nucleotides Psyr_0257: Psyr_0257 - RNA binding S1, at 271,515 to 273,833 _0257 Psyr_0258: Psyr_0258 - Two-component response regulator OmpR, at 274,133 to 274,873 _0258 Psyr_0259: Psyr_0259 - Osmolarity sensor protein envZ, at 275,025 to 276,347 _0259 Position (kb) 274 275Strain fitness (log2 ratio) -2 -1 0 1 2at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.378 kb on - strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.656 kb on - strandat 273.656 kb on - strandat 273.760 kb on - strandat 273.793 kb on - strandat 273.834 kb on - strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.873 kb on - strandat 273.873 kb on - strandat 273.909 kb on + strandat 273.970 kb on + strandat 273.970 kb on + strandat 273.971 kb on - strandat 273.973 kb on - strandat 273.990 kb on + strandat 274.096 kb on + strandat 274.096 kb on + strandat 274.915 kb on + strandat 274.915 kb on + strandat 274.916 kb on - strandat 274.947 kb on + strandat 274.948 kb on - strandat 275.024 kb on + strandat 275.024 kb on + strandat 275.024 kb on + strandat 275.024 kb on + strandat 275.024 kb on + strandat 275.025 kb on - strandat 275.025 kb on - strandat 275.025 kb on - strandat 275.036 kb on + strandat 275.036 kb on + strandat 275.036 kb on + strandat 275.320 kb on - strand, within Psyr_0259

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Cimicifugoside H1 10 mM
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273,349 + Psyr_0257 0.79 -1.4
273,349 + Psyr_0257 0.79 -0.7
273,349 + Psyr_0257 0.79 -0.2
273,349 + Psyr_0257 0.79 +0.3
273,350 - Psyr_0257 0.79 -0.5
273,350 - Psyr_0257 0.79 -1.0
273,350 - Psyr_0257 0.79 -0.7
273,350 - Psyr_0257 0.79 +0.4
273,350 - Psyr_0257 0.79 +0.7
273,350 - Psyr_0257 0.79 -1.0
273,350 - Psyr_0257 0.79 +1.5
273,375 + Psyr_0257 0.80 +0.5
273,375 + Psyr_0257 0.80 +0.4
273,375 + Psyr_0257 0.80 -0.1
273,375 + Psyr_0257 0.80 -1.2
273,375 + Psyr_0257 0.80 -0.2
273,375 + Psyr_0257 0.80 +0.4
273,375 + Psyr_0257 0.80 -0.1
273,375 + Psyr_0257 0.80 +1.3
273,375 + Psyr_0257 0.80 -0.6
273,376 - Psyr_0257 0.80 +0.3
273,376 - Psyr_0257 0.80 +0.6
273,376 - Psyr_0257 0.80 -0.0
273,376 - Psyr_0257 0.80 +1.7
273,376 - Psyr_0257 0.80 -0.7
273,376 - Psyr_0257 0.80 +0.1
273,376 - Psyr_0257 0.80 -0.3
273,376 - Psyr_0257 0.80 -0.3
273,376 - Psyr_0257 0.80 +0.3
273,378 - Psyr_0257 0.80 -0.7
273,518 + Psyr_0257 0.86 +0.6
273,518 + Psyr_0257 0.86 -1.1
273,518 + Psyr_0257 0.86 -0.7
273,518 + Psyr_0257 0.86 -0.8
273,518 + Psyr_0257 0.86 -1.5
273,518 + Psyr_0257 0.86 +2.5
273,519 - Psyr_0257 0.86 +0.6
273,519 - Psyr_0257 0.86 +0.3
273,519 - Psyr_0257 0.86 +0.0
273,519 - Psyr_0257 0.86 -0.3
273,519 - Psyr_0257 0.86 +0.9
273,519 - Psyr_0257 0.86 +1.2
273,521 - Psyr_0257 0.87 +0.1
273,521 - Psyr_0257 0.87 -0.8
273,521 - Psyr_0257 0.87 -1.8
273,521 - Psyr_0257 0.87 -0.7
273,529 + Psyr_0257 0.87 -1.8
273,529 + Psyr_0257 0.87 -0.6
273,529 + Psyr_0257 0.87 +0.7
273,529 + Psyr_0257 0.87 +0.5
273,529 + Psyr_0257 0.87 +0.0
273,530 - Psyr_0257 0.87 -0.8
273,530 - Psyr_0257 0.87 -0.3
273,530 - Psyr_0257 0.87 -0.3
273,530 - Psyr_0257 0.87 -0.2
273,656 - +0.7
273,656 - +0.1
273,760 - -0.2
273,793 - -0.6
273,834 - +0.2
273,868 + -1.3
273,868 + +0.2
273,868 + -0.4
273,868 + +0.3
273,868 + +0.0
273,868 + -1.6
273,868 + -0.4
273,868 + -0.5
273,868 + +0.0
273,869 - +1.3
273,869 - -1.1
273,869 - -0.3
273,869 - +0.1
273,869 - -0.9
273,872 + +1.3
273,872 + +0.4
273,872 + +0.1
273,872 + -1.3
273,873 - -0.5
273,873 - -0.3
273,909 + +0.4
273,970 + +0.1
273,970 + +0.3
273,971 - +1.3
273,973 - +0.4
273,990 + -0.2
274,096 + -0.8
274,096 + +0.6
274,915 + -0.3
274,915 + +0.1
274,916 - -0.6
274,947 + +1.0
274,948 - +0.1
275,024 + +0.1
275,024 + -0.9
275,024 + -0.5
275,024 + +0.1
275,024 + +0.2
275,025 - -1.8
275,025 - +0.7
275,025 - +0.2
275,036 + -0.1
275,036 + -0.9
275,036 + -0.1
275,320 - Psyr_0259 0.22 -1.7

Or see this region's nucleotide sequence